Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17680 and BBR_RS17685 are separated by 156 nucleotides BBR_RS17685 and BBR_RS17690 are separated by 459 nucleotides BBR_RS17690 and BBR_RS17695 are separated by 56 nucleotides
BBR_RS17680: BBR_RS17680 - hypothetical protein, at 1,747,720 to 1,747,947
_RS17680
BBR_RS17685: BBR_RS17685 - hypothetical protein, at 1,748,104 to 1,748,337
_RS17685
BBR_RS17690: BBR_RS17690 - CRISPR-associated endonuclease Cas2, at 1,748,797 to 1,749,087
_RS17690
BBR_RS17695: BBR_RS17695 - type I-C CRISPR-associated endonuclease Cas1, at 1,749,144 to 1,750,175
_RS17695
Position (kb)
1748
1749 Strain fitness (log2 ratio)
-1
0
1 at 1747.159 kb on + strand at 1747.162 kb on + strand at 1747.167 kb on - strand at 1747.244 kb on - strand at 1747.259 kb on + strand at 1747.274 kb on - strand at 1747.301 kb on + strand at 1747.352 kb on + strand at 1747.381 kb on + strand at 1747.398 kb on + strand at 1747.406 kb on - strand at 1747.408 kb on + strand at 1747.469 kb on - strand at 1747.469 kb on - strand at 1747.469 kb on - strand at 1747.469 kb on - strand at 1747.497 kb on + strand at 1747.525 kb on - strand at 1747.558 kb on - strand at 1747.564 kb on + strand at 1747.564 kb on + strand at 1747.564 kb on + strand at 1747.564 kb on + strand at 1747.572 kb on - strand at 1747.572 kb on - strand at 1747.598 kb on - strand at 1747.615 kb on + strand at 1747.630 kb on - strand at 1747.630 kb on - strand at 1747.670 kb on - strand at 1747.670 kb on - strand at 1747.706 kb on - strand at 1747.722 kb on + strand at 1747.730 kb on - strand at 1747.739 kb on - strand at 1747.739 kb on - strand at 1747.746 kb on - strand, within BBR_RS17680 at 1747.838 kb on + strand, within BBR_RS17680 at 1747.840 kb on - strand, within BBR_RS17680 at 1747.841 kb on + strand, within BBR_RS17680 at 1747.883 kb on + strand, within BBR_RS17680 at 1747.883 kb on + strand, within BBR_RS17680 at 1747.955 kb on + strand at 1747.955 kb on + strand at 1747.968 kb on - strand at 1747.993 kb on - strand at 1748.014 kb on - strand at 1748.027 kb on + strand at 1748.027 kb on + strand at 1748.056 kb on + strand at 1748.065 kb on + strand at 1748.065 kb on - strand at 1748.072 kb on + strand at 1748.100 kb on - strand at 1748.121 kb on - strand at 1748.121 kb on - strand at 1748.121 kb on - strand at 1748.160 kb on + strand, within BBR_RS17685 at 1748.203 kb on - strand, within BBR_RS17685 at 1748.203 kb on - strand, within BBR_RS17685 at 1748.209 kb on - strand, within BBR_RS17685 at 1748.228 kb on + strand, within BBR_RS17685 at 1748.249 kb on + strand, within BBR_RS17685 at 1748.256 kb on - strand, within BBR_RS17685 at 1748.271 kb on - strand, within BBR_RS17685 at 1748.340 kb on - strand at 1748.347 kb on - strand at 1748.382 kb on + strand at 1748.394 kb on + strand at 1748.402 kb on - strand at 1748.402 kb on - strand at 1748.412 kb on - strand at 1748.422 kb on + strand at 1748.432 kb on + strand at 1748.435 kb on - strand at 1748.435 kb on - strand at 1748.440 kb on - strand at 1748.471 kb on - strand at 1748.471 kb on - strand at 1748.513 kb on - strand at 1748.542 kb on - strand at 1748.616 kb on + strand at 1748.623 kb on - strand at 1748.626 kb on + strand at 1748.634 kb on - strand at 1748.634 kb on - strand at 1748.634 kb on - strand at 1748.676 kb on + strand at 1748.701 kb on - strand at 1748.710 kb on + strand at 1748.710 kb on + strand at 1748.711 kb on - strand at 1748.752 kb on - strand at 1748.813 kb on - strand at 1748.826 kb on + strand, within BBR_RS17690 at 1748.827 kb on + strand, within BBR_RS17690 at 1748.835 kb on + strand, within BBR_RS17690 at 1748.835 kb on - strand, within BBR_RS17690 at 1748.886 kb on + strand, within BBR_RS17690 at 1748.894 kb on + strand, within BBR_RS17690 at 1748.910 kb on - strand, within BBR_RS17690 at 1748.955 kb on + strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1749.018 kb on - strand, within BBR_RS17690 at 1749.018 kb on - strand, within BBR_RS17690 at 1749.042 kb on - strand, within BBR_RS17690 at 1749.043 kb on - strand, within BBR_RS17690 at 1749.064 kb on - strand at 1749.064 kb on - strand at 1749.065 kb on + strand at 1749.087 kb on + strand at 1749.129 kb on - strand at 1749.201 kb on - strand at 1749.217 kb on + strand at 1749.227 kb on - strand at 1749.235 kb on - strand at 1749.313 kb on - strand, within BBR_RS17695 at 1749.330 kb on - strand, within BBR_RS17695
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,747,159 + +0.0 1,747,162 + -0.2 1,747,167 - +0.2 1,747,244 - +0.3 1,747,259 + +0.5 1,747,274 - +0.8 1,747,301 + -0.5 1,747,352 + +0.5 1,747,381 + -0.3 1,747,398 + -0.4 1,747,406 - -1.2 1,747,408 + +1.4 1,747,469 - +0.1 1,747,469 - +0.5 1,747,469 - -0.6 1,747,469 - +0.2 1,747,497 + -0.9 1,747,525 - +0.4 1,747,558 - +0.4 1,747,564 + +0.5 1,747,564 + +0.1 1,747,564 + -1.1 1,747,564 + +0.3 1,747,572 - -0.2 1,747,572 - +0.6 1,747,598 - -0.7 1,747,615 + -0.2 1,747,630 - -0.2 1,747,630 - +0.2 1,747,670 - +0.5 1,747,670 - +0.5 1,747,706 - -1.1 1,747,722 + -1.2 1,747,730 - -0.9 1,747,739 - +0.3 1,747,739 - +0.2 1,747,746 - BBR_RS17680 0.11 -0.2 1,747,838 + BBR_RS17680 0.52 +0.2 1,747,840 - BBR_RS17680 0.53 -0.5 1,747,841 + BBR_RS17680 0.53 -0.0 1,747,883 + BBR_RS17680 0.71 -0.5 1,747,883 + BBR_RS17680 0.71 +0.0 1,747,955 + -0.0 1,747,955 + +0.1 1,747,968 - +0.7 1,747,993 - -0.2 1,748,014 - +0.4 1,748,027 + +0.1 1,748,027 + +0.6 1,748,056 + +0.8 1,748,065 + -0.6 1,748,065 - -0.6 1,748,072 + -0.1 1,748,100 - -0.4 1,748,121 - +1.2 1,748,121 - +0.6 1,748,121 - +0.0 1,748,160 + BBR_RS17685 0.24 -0.0 1,748,203 - BBR_RS17685 0.42 +0.9 1,748,203 - BBR_RS17685 0.42 +0.1 1,748,209 - BBR_RS17685 0.45 +0.1 1,748,228 + BBR_RS17685 0.53 +0.5 1,748,249 + BBR_RS17685 0.62 +1.5 1,748,256 - BBR_RS17685 0.65 +0.7 1,748,271 - BBR_RS17685 0.71 +0.2 1,748,340 - -0.5 1,748,347 - -0.4 1,748,382 + +0.8 1,748,394 + +0.1 1,748,402 - -0.2 1,748,402 - +0.3 1,748,412 - +0.9 1,748,422 + -0.2 1,748,432 + +0.8 1,748,435 - +0.5 1,748,435 - -1.7 1,748,440 - -0.8 1,748,471 - +0.9 1,748,471 - -0.7 1,748,513 - +0.1 1,748,542 - -0.3 1,748,616 + +0.1 1,748,623 - +0.2 1,748,626 + -0.5 1,748,634 - -0.5 1,748,634 - -0.3 1,748,634 - +0.2 1,748,676 + +1.6 1,748,701 - +0.6 1,748,710 + -0.8 1,748,710 + +0.9 1,748,711 - +0.1 1,748,752 - -0.0 1,748,813 - +0.6 1,748,826 + BBR_RS17690 0.10 -0.4 1,748,827 + BBR_RS17690 0.10 -1.0 1,748,835 + BBR_RS17690 0.13 -0.9 1,748,835 - BBR_RS17690 0.13 +0.3 1,748,886 + BBR_RS17690 0.31 +0.9 1,748,894 + BBR_RS17690 0.33 +0.2 1,748,910 - BBR_RS17690 0.39 -0.0 1,748,955 + BBR_RS17690 0.54 +1.3 1,748,963 - BBR_RS17690 0.57 -0.1 1,748,963 - BBR_RS17690 0.57 -0.0 1,748,963 - BBR_RS17690 0.57 -0.2 1,749,018 - BBR_RS17690 0.76 +0.3 1,749,018 - BBR_RS17690 0.76 -0.5 1,749,042 - BBR_RS17690 0.84 +0.7 1,749,043 - BBR_RS17690 0.85 -0.9 1,749,064 - -0.6 1,749,064 - +0.9 1,749,065 + +0.2 1,749,087 + -0.5 1,749,129 - +0.1 1,749,201 - +0.1 1,749,217 + +0.8 1,749,227 - -0.7 1,749,235 - -0.2 1,749,313 - BBR_RS17695 0.16 -0.1 1,749,330 - BBR_RS17695 0.18 -0.1
Or see this region's nucleotide sequence