Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17510 and BBR_RS17515 overlap by 4 nucleotides BBR_RS17515 and BBR_RS17520 are separated by 126 nucleotides BBR_RS17520 and BBR_RS17525 overlap by 4 nucleotides
BBR_RS17510: BBR_RS17510 - VWA domain-containing protein, at 1,706,966 to 1,708,003
_RS17510
BBR_RS17515: BBR_RS17515 - hypothetical protein, at 1,708,000 to 1,708,572
_RS17515
BBR_RS17520: BBR_RS17520 - hypothetical protein, at 1,708,699 to 1,709,007
_RS17520
BBR_RS17525: BBR_RS17525 - hypothetical protein, at 1,709,004 to 1,710,584
_RS17525
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1707.708 kb on + strand, within BBR_RS17510 at 1707.718 kb on + strand, within BBR_RS17510 at 1707.774 kb on + strand, within BBR_RS17510 at 1707.784 kb on - strand, within BBR_RS17510 at 1707.784 kb on - strand, within BBR_RS17510 at 1707.804 kb on + strand, within BBR_RS17510 at 1707.815 kb on + strand, within BBR_RS17510 at 1707.882 kb on - strand, within BBR_RS17510 at 1707.907 kb on + strand at 1707.912 kb on + strand at 1707.914 kb on + strand at 1707.917 kb on - strand at 1707.919 kb on + strand at 1707.919 kb on + strand at 1707.919 kb on + strand at 1707.920 kb on - strand at 1707.927 kb on - strand at 1707.997 kb on + strand at 1708.007 kb on + strand at 1708.007 kb on + strand at 1708.017 kb on - strand at 1708.017 kb on - strand at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.103 kb on + strand, within BBR_RS17515 at 1708.111 kb on - strand, within BBR_RS17515 at 1708.111 kb on - strand, within BBR_RS17515 at 1708.169 kb on - strand, within BBR_RS17515 at 1708.203 kb on - strand, within BBR_RS17515 at 1708.213 kb on - strand, within BBR_RS17515 at 1708.213 kb on - strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.242 kb on + strand, within BBR_RS17515 at 1708.310 kb on + strand, within BBR_RS17515 at 1708.310 kb on + strand, within BBR_RS17515 at 1708.318 kb on - strand, within BBR_RS17515 at 1708.318 kb on - strand, within BBR_RS17515 at 1708.331 kb on + strand, within BBR_RS17515 at 1708.341 kb on + strand, within BBR_RS17515 at 1708.369 kb on + strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.377 kb on - strand, within BBR_RS17515 at 1708.382 kb on - strand, within BBR_RS17515 at 1708.392 kb on - strand, within BBR_RS17515 at 1708.418 kb on - strand, within BBR_RS17515 at 1708.484 kb on - strand, within BBR_RS17515 at 1708.564 kb on - strand at 1708.564 kb on - strand at 1708.577 kb on - strand at 1708.612 kb on + strand at 1708.612 kb on + strand at 1708.645 kb on - strand at 1708.654 kb on - strand at 1708.660 kb on + strand at 1708.683 kb on - strand at 1708.684 kb on - strand at 1708.691 kb on - strand at 1708.708 kb on + strand at 1708.718 kb on + strand at 1708.727 kb on + strand at 1708.800 kb on - strand, within BBR_RS17520 at 1708.810 kb on - strand, within BBR_RS17520 at 1708.810 kb on - strand, within BBR_RS17520 at 1708.827 kb on + strand, within BBR_RS17520 at 1708.827 kb on + strand, within BBR_RS17520 at 1708.836 kb on - strand, within BBR_RS17520 at 1708.883 kb on + strand, within BBR_RS17520 at 1708.893 kb on + strand, within BBR_RS17520 at 1708.962 kb on - strand, within BBR_RS17520 at 1708.963 kb on + strand, within BBR_RS17520 at 1708.975 kb on - strand, within BBR_RS17520 at 1708.998 kb on - strand at 1709.069 kb on + strand at 1709.076 kb on + strand at 1709.145 kb on - strand at 1709.190 kb on + strand, within BBR_RS17525 at 1709.190 kb on + strand, within BBR_RS17525 at 1709.206 kb on - strand, within BBR_RS17525 at 1709.224 kb on - strand, within BBR_RS17525 at 1709.231 kb on + strand, within BBR_RS17525 at 1709.269 kb on - strand, within BBR_RS17525 at 1709.278 kb on + strand, within BBR_RS17525 at 1709.286 kb on - strand, within BBR_RS17525 at 1709.403 kb on + strand, within BBR_RS17525 at 1709.411 kb on + strand, within BBR_RS17525 at 1709.419 kb on - strand, within BBR_RS17525 at 1709.423 kb on + strand, within BBR_RS17525 at 1709.450 kb on - strand, within BBR_RS17525 at 1709.512 kb on - strand, within BBR_RS17525 at 1709.522 kb on - strand, within BBR_RS17525 at 1709.530 kb on - strand, within BBR_RS17525 at 1709.590 kb on + strand, within BBR_RS17525 at 1709.590 kb on + strand, within BBR_RS17525 at 1709.598 kb on - strand, within BBR_RS17525 at 1709.608 kb on - strand, within BBR_RS17525 at 1709.663 kb on + strand, within BBR_RS17525 at 1709.689 kb on + strand, within BBR_RS17525 at 1709.697 kb on + strand, within BBR_RS17525 at 1709.706 kb on + strand, within BBR_RS17525 at 1709.760 kb on - strand, within BBR_RS17525 at 1709.823 kb on - strand, within BBR_RS17525 at 1709.828 kb on - strand, within BBR_RS17525 at 1709.846 kb on - strand, within BBR_RS17525 at 1709.867 kb on - strand, within BBR_RS17525 at 1709.923 kb on + strand, within BBR_RS17525 at 1709.953 kb on + strand, within BBR_RS17525 at 1709.961 kb on - strand, within BBR_RS17525 at 1709.971 kb on + strand, within BBR_RS17525
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,707,708 + BBR_RS17510 0.71 -2.5 1,707,718 + BBR_RS17510 0.72 -2.2 1,707,774 + BBR_RS17510 0.78 -1.5 1,707,784 - BBR_RS17510 0.79 -1.0 1,707,784 - BBR_RS17510 0.79 -0.5 1,707,804 + BBR_RS17510 0.81 -0.9 1,707,815 + BBR_RS17510 0.82 -0.8 1,707,882 - BBR_RS17510 0.88 -1.2 1,707,907 + -1.5 1,707,912 + -1.0 1,707,914 + -2.5 1,707,917 - -1.5 1,707,919 + +0.2 1,707,919 + -2.8 1,707,919 + -2.3 1,707,920 - +0.4 1,707,927 - -1.3 1,707,997 + -1.4 1,708,007 + -0.5 1,708,007 + -1.9 1,708,017 - -0.3 1,708,017 - -2.2 1,708,103 + BBR_RS17515 0.18 -1.7 1,708,103 + BBR_RS17515 0.18 -2.1 1,708,103 + BBR_RS17515 0.18 -0.4 1,708,103 + BBR_RS17515 0.18 -1.3 1,708,111 - BBR_RS17515 0.19 -1.4 1,708,111 - BBR_RS17515 0.19 -0.8 1,708,169 - BBR_RS17515 0.29 +0.4 1,708,203 - BBR_RS17515 0.35 -0.7 1,708,213 - BBR_RS17515 0.37 -0.1 1,708,213 - BBR_RS17515 0.37 -0.9 1,708,242 + BBR_RS17515 0.42 -2.2 1,708,242 + BBR_RS17515 0.42 -3.4 1,708,242 + BBR_RS17515 0.42 -0.2 1,708,310 + BBR_RS17515 0.54 -1.4 1,708,310 + BBR_RS17515 0.54 -0.6 1,708,318 - BBR_RS17515 0.55 -2.3 1,708,318 - BBR_RS17515 0.55 -1.7 1,708,331 + BBR_RS17515 0.58 -1.3 1,708,341 + BBR_RS17515 0.60 -0.7 1,708,369 + BBR_RS17515 0.64 -2.4 1,708,377 - BBR_RS17515 0.66 -0.5 1,708,377 - BBR_RS17515 0.66 -0.4 1,708,377 - BBR_RS17515 0.66 -2.5 1,708,377 - BBR_RS17515 0.66 -1.0 1,708,382 - BBR_RS17515 0.67 -1.5 1,708,392 - BBR_RS17515 0.68 -0.8 1,708,418 - BBR_RS17515 0.73 -1.1 1,708,484 - BBR_RS17515 0.84 -2.5 1,708,564 - -1.0 1,708,564 - -0.6 1,708,577 - +0.4 1,708,612 + +0.2 1,708,612 + -0.4 1,708,645 - -0.4 1,708,654 - -0.5 1,708,660 + +0.1 1,708,683 - -1.2 1,708,684 - +0.9 1,708,691 - -0.0 1,708,708 + -0.2 1,708,718 + -0.1 1,708,727 + +0.0 1,708,800 - BBR_RS17520 0.33 -0.3 1,708,810 - BBR_RS17520 0.36 +0.5 1,708,810 - BBR_RS17520 0.36 +0.3 1,708,827 + BBR_RS17520 0.41 +0.8 1,708,827 + BBR_RS17520 0.41 +0.7 1,708,836 - BBR_RS17520 0.44 +0.5 1,708,883 + BBR_RS17520 0.60 -0.5 1,708,893 + BBR_RS17520 0.63 +0.2 1,708,962 - BBR_RS17520 0.85 +0.1 1,708,963 + BBR_RS17520 0.85 -0.4 1,708,975 - BBR_RS17520 0.89 +0.1 1,708,998 - -0.0 1,709,069 + -0.3 1,709,076 + -1.2 1,709,145 - +1.1 1,709,190 + BBR_RS17525 0.12 -0.1 1,709,190 + BBR_RS17525 0.12 +0.4 1,709,206 - BBR_RS17525 0.13 +0.1 1,709,224 - BBR_RS17525 0.14 -0.7 1,709,231 + BBR_RS17525 0.14 +0.0 1,709,269 - BBR_RS17525 0.17 -0.2 1,709,278 + BBR_RS17525 0.17 -0.3 1,709,286 - BBR_RS17525 0.18 +0.1 1,709,403 + BBR_RS17525 0.25 +0.4 1,709,411 + BBR_RS17525 0.26 -0.4 1,709,419 - BBR_RS17525 0.26 -0.4 1,709,423 + BBR_RS17525 0.27 -0.3 1,709,450 - BBR_RS17525 0.28 -0.8 1,709,512 - BBR_RS17525 0.32 -0.2 1,709,522 - BBR_RS17525 0.33 -1.4 1,709,530 - BBR_RS17525 0.33 -0.2 1,709,590 + BBR_RS17525 0.37 -0.2 1,709,590 + BBR_RS17525 0.37 +0.1 1,709,598 - BBR_RS17525 0.38 +0.4 1,709,608 - BBR_RS17525 0.38 +0.7 1,709,663 + BBR_RS17525 0.42 -0.1 1,709,689 + BBR_RS17525 0.43 +0.0 1,709,697 + BBR_RS17525 0.44 +0.3 1,709,706 + BBR_RS17525 0.44 +0.9 1,709,760 - BBR_RS17525 0.48 -1.1 1,709,823 - BBR_RS17525 0.52 -0.3 1,709,828 - BBR_RS17525 0.52 +0.6 1,709,846 - BBR_RS17525 0.53 +0.4 1,709,867 - BBR_RS17525 0.55 -0.9 1,709,923 + BBR_RS17525 0.58 +0.0 1,709,953 + BBR_RS17525 0.60 +0.5 1,709,961 - BBR_RS17525 0.61 -0.2 1,709,971 + BBR_RS17525 0.61 -0.6
Or see this region's nucleotide sequence