Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17030 and BBR_RS17035 are separated by 23 nucleotides BBR_RS17035 and BBR_RS17040 are separated by 30 nucleotides BBR_RS17040 and BBR_RS17045 overlap by 1 nucleotides BBR_RS17045 and BBR_RS17050 are separated by 77 nucleotides
BBR_RS17030: BBR_RS17030 - bifunctional 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase/phosphoribosylanthranilate isomerase PriA, at 1,596,902 to 1,597,627
_RS17030
BBR_RS17035: BBR_RS17035 - imidazole glycerol phosphate synthase subunit HisH, at 1,597,651 to 1,598,298
_RS17035
BBR_RS17040: BBR_RS17040 - hypothetical protein, at 1,598,329 to 1,599,075
_RS17040
BBR_RS17045: BBR_RS17045 - imidazoleglycerol-phosphate dehydratase, at 1,599,075 to 1,599,674
_RS17045
BBR_RS17050: BBR_RS17050 - histidinol-phosphate transaminase, at 1,599,752 to 1,600,912
_RS17050
Position (kb)
1598
1599
1600 Strain fitness (log2 ratio)
-2
-1
0
1 at 1597.413 kb on - strand, within BBR_RS17030 at 1597.430 kb on - strand, within BBR_RS17030 at 1597.442 kb on + strand, within BBR_RS17030 at 1597.471 kb on - strand, within BBR_RS17030 at 1597.484 kb on + strand, within BBR_RS17030 at 1597.500 kb on + strand, within BBR_RS17030 at 1597.543 kb on - strand, within BBR_RS17030 at 1597.597 kb on + strand at 1597.602 kb on + strand at 1597.605 kb on - strand at 1597.656 kb on - strand at 1597.709 kb on + strand at 1597.767 kb on + strand, within BBR_RS17035 at 1597.768 kb on + strand, within BBR_RS17035 at 1597.768 kb on + strand, within BBR_RS17035 at 1597.804 kb on - strand, within BBR_RS17035 at 1597.871 kb on + strand, within BBR_RS17035 at 1597.879 kb on - strand, within BBR_RS17035 at 1597.903 kb on - strand, within BBR_RS17035 at 1597.905 kb on + strand, within BBR_RS17035 at 1597.905 kb on + strand, within BBR_RS17035 at 1598.000 kb on - strand, within BBR_RS17035 at 1598.051 kb on + strand, within BBR_RS17035 at 1598.059 kb on - strand, within BBR_RS17035 at 1598.071 kb on - strand, within BBR_RS17035 at 1598.100 kb on - strand, within BBR_RS17035 at 1598.138 kb on - strand, within BBR_RS17035 at 1598.166 kb on + strand, within BBR_RS17035 at 1598.219 kb on - strand, within BBR_RS17035 at 1598.252 kb on + strand at 1598.271 kb on - strand at 1598.293 kb on + strand at 1598.294 kb on + strand at 1598.302 kb on - strand at 1598.302 kb on - strand at 1598.302 kb on - strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.304 kb on + strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.312 kb on - strand at 1598.314 kb on + strand at 1598.314 kb on + strand at 1598.319 kb on + strand at 1598.322 kb on - strand at 1598.322 kb on - strand at 1598.357 kb on + strand at 1598.363 kb on + strand at 1598.371 kb on - strand at 1598.442 kb on - strand, within BBR_RS17040 at 1598.509 kb on - strand, within BBR_RS17040 at 1598.509 kb on - strand, within BBR_RS17040 at 1598.532 kb on + strand, within BBR_RS17040 at 1598.532 kb on + strand, within BBR_RS17040 at 1598.552 kb on + strand, within BBR_RS17040 at 1598.560 kb on - strand, within BBR_RS17040 at 1598.560 kb on - strand, within BBR_RS17040 at 1598.601 kb on - strand, within BBR_RS17040 at 1598.601 kb on - strand, within BBR_RS17040 at 1598.628 kb on - strand, within BBR_RS17040 at 1598.646 kb on - strand, within BBR_RS17040 at 1598.648 kb on - strand, within BBR_RS17040 at 1598.711 kb on - strand, within BBR_RS17040 at 1598.827 kb on + strand, within BBR_RS17040 at 1598.856 kb on - strand, within BBR_RS17040 at 1598.887 kb on - strand, within BBR_RS17040 at 1598.887 kb on - strand, within BBR_RS17040 at 1599.007 kb on - strand at 1599.007 kb on - strand at 1599.017 kb on - strand at 1599.083 kb on - strand at 1599.108 kb on - strand at 1599.108 kb on - strand at 1599.143 kb on - strand, within BBR_RS17045 at 1599.143 kb on - strand, within BBR_RS17045 at 1599.147 kb on + strand, within BBR_RS17045 at 1599.155 kb on - strand, within BBR_RS17045 at 1599.155 kb on - strand, within BBR_RS17045 at 1599.155 kb on - strand, within BBR_RS17045 at 1599.162 kb on - strand, within BBR_RS17045 at 1599.212 kb on - strand, within BBR_RS17045 at 1599.302 kb on - strand, within BBR_RS17045 at 1599.328 kb on + strand, within BBR_RS17045 at 1599.336 kb on - strand, within BBR_RS17045 at 1599.363 kb on + strand, within BBR_RS17045 at 1599.378 kb on - strand, within BBR_RS17045 at 1599.432 kb on - strand, within BBR_RS17045 at 1599.465 kb on + strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.473 kb on - strand, within BBR_RS17045 at 1599.475 kb on + strand, within BBR_RS17045 at 1599.475 kb on - strand, within BBR_RS17045 at 1599.477 kb on + strand, within BBR_RS17045 at 1599.483 kb on - strand, within BBR_RS17045 at 1599.483 kb on - strand, within BBR_RS17045 at 1599.485 kb on - strand, within BBR_RS17045 at 1599.485 kb on - strand, within BBR_RS17045 at 1599.537 kb on - strand, within BBR_RS17045 at 1599.572 kb on + strand, within BBR_RS17045 at 1599.585 kb on - strand, within BBR_RS17045 at 1599.613 kb on - strand, within BBR_RS17045 at 1599.729 kb on + strand at 1599.732 kb on - strand at 1599.749 kb on + strand at 1599.757 kb on - strand at 1599.767 kb on - strand at 1599.768 kb on + strand at 1599.768 kb on + strand at 1599.776 kb on - strand at 1599.822 kb on - strand at 1599.865 kb on + strand at 1599.955 kb on - strand, within BBR_RS17050 at 1599.958 kb on + strand, within BBR_RS17050 at 1599.958 kb on + strand, within BBR_RS17050 at 1599.958 kb on + strand, within BBR_RS17050 at 1599.973 kb on + strand, within BBR_RS17050 at 1599.980 kb on + strand, within BBR_RS17050 at 1599.988 kb on - strand, within BBR_RS17050 at 1599.988 kb on - strand, within BBR_RS17050 at 1599.988 kb on - strand, within BBR_RS17050 at 1600.008 kb on + strand, within BBR_RS17050 at 1600.020 kb on + strand, within BBR_RS17050 at 1600.035 kb on - strand, within BBR_RS17050 at 1600.048 kb on - strand, within BBR_RS17050
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,597,413 - BBR_RS17030 0.70 -1.4 1,597,430 - BBR_RS17030 0.73 -0.1 1,597,442 + BBR_RS17030 0.74 -0.5 1,597,471 - BBR_RS17030 0.78 -0.4 1,597,484 + BBR_RS17030 0.80 +0.0 1,597,500 + BBR_RS17030 0.82 -0.2 1,597,543 - BBR_RS17030 0.88 +0.3 1,597,597 + +0.1 1,597,602 + +1.0 1,597,605 - -0.7 1,597,656 - +0.4 1,597,709 + -0.1 1,597,767 + BBR_RS17035 0.18 -0.8 1,597,768 + BBR_RS17035 0.18 -0.3 1,597,768 + BBR_RS17035 0.18 -0.0 1,597,804 - BBR_RS17035 0.24 -0.0 1,597,871 + BBR_RS17035 0.34 -1.6 1,597,879 - BBR_RS17035 0.35 -0.7 1,597,903 - BBR_RS17035 0.39 -0.7 1,597,905 + BBR_RS17035 0.39 +0.1 1,597,905 + BBR_RS17035 0.39 +0.3 1,598,000 - BBR_RS17035 0.54 -1.0 1,598,051 + BBR_RS17035 0.62 +0.6 1,598,059 - BBR_RS17035 0.63 -1.3 1,598,071 - BBR_RS17035 0.65 +0.4 1,598,100 - BBR_RS17035 0.69 +0.3 1,598,138 - BBR_RS17035 0.75 +0.6 1,598,166 + BBR_RS17035 0.79 -0.5 1,598,219 - BBR_RS17035 0.88 -1.3 1,598,252 + -0.2 1,598,271 - -0.5 1,598,293 + -0.3 1,598,294 + +0.2 1,598,302 - +0.2 1,598,302 - -0.1 1,598,302 - +1.1 1,598,304 + -0.9 1,598,304 + +0.7 1,598,304 + +0.1 1,598,304 + -1.1 1,598,304 + -0.1 1,598,304 + -0.2 1,598,304 + -1.1 1,598,304 + +0.6 1,598,312 - -0.0 1,598,312 - +0.1 1,598,312 - +0.5 1,598,312 - -0.2 1,598,312 - +0.3 1,598,312 - -0.1 1,598,312 - -0.5 1,598,312 - +0.3 1,598,314 + +0.5 1,598,314 + +0.8 1,598,319 + -0.1 1,598,322 - -0.7 1,598,322 - +0.2 1,598,357 + +0.2 1,598,363 + -1.1 1,598,371 - +1.1 1,598,442 - BBR_RS17040 0.15 +0.6 1,598,509 - BBR_RS17040 0.24 -0.1 1,598,509 - BBR_RS17040 0.24 +1.7 1,598,532 + BBR_RS17040 0.27 +0.3 1,598,532 + BBR_RS17040 0.27 +0.7 1,598,552 + BBR_RS17040 0.30 +0.1 1,598,560 - BBR_RS17040 0.31 -0.1 1,598,560 - BBR_RS17040 0.31 -0.4 1,598,601 - BBR_RS17040 0.36 +1.0 1,598,601 - BBR_RS17040 0.36 -2.5 1,598,628 - BBR_RS17040 0.40 +0.6 1,598,646 - BBR_RS17040 0.42 +0.3 1,598,648 - BBR_RS17040 0.43 +0.8 1,598,711 - BBR_RS17040 0.51 +0.1 1,598,827 + BBR_RS17040 0.67 -0.8 1,598,856 - BBR_RS17040 0.71 +0.3 1,598,887 - BBR_RS17040 0.75 +0.3 1,598,887 - BBR_RS17040 0.75 +0.1 1,599,007 - -0.5 1,599,007 - +0.3 1,599,017 - +0.7 1,599,083 - -0.3 1,599,108 - -0.2 1,599,108 - +0.4 1,599,143 - BBR_RS17045 0.11 -0.8 1,599,143 - BBR_RS17045 0.11 -0.0 1,599,147 + BBR_RS17045 0.12 +0.4 1,599,155 - BBR_RS17045 0.13 -0.4 1,599,155 - BBR_RS17045 0.13 +0.9 1,599,155 - BBR_RS17045 0.13 +0.2 1,599,162 - BBR_RS17045 0.14 +0.3 1,599,212 - BBR_RS17045 0.23 -0.4 1,599,302 - BBR_RS17045 0.38 -0.8 1,599,328 + BBR_RS17045 0.42 -0.3 1,599,336 - BBR_RS17045 0.43 +0.6 1,599,363 + BBR_RS17045 0.48 +0.2 1,599,378 - BBR_RS17045 0.51 +0.6 1,599,432 - BBR_RS17045 0.59 -0.0 1,599,465 + BBR_RS17045 0.65 -0.3 1,599,473 - BBR_RS17045 0.66 +1.3 1,599,473 - BBR_RS17045 0.66 +0.2 1,599,473 - BBR_RS17045 0.66 -0.2 1,599,473 - BBR_RS17045 0.66 +0.4 1,599,473 - BBR_RS17045 0.66 -0.5 1,599,473 - BBR_RS17045 0.66 +0.3 1,599,473 - BBR_RS17045 0.66 +0.1 1,599,473 - BBR_RS17045 0.66 -1.3 1,599,475 + BBR_RS17045 0.67 +0.5 1,599,475 - BBR_RS17045 0.67 +0.7 1,599,477 + BBR_RS17045 0.67 +0.7 1,599,483 - BBR_RS17045 0.68 +0.5 1,599,483 - BBR_RS17045 0.68 +0.1 1,599,485 - BBR_RS17045 0.68 -0.0 1,599,485 - BBR_RS17045 0.68 -0.0 1,599,537 - BBR_RS17045 0.77 +0.3 1,599,572 + BBR_RS17045 0.83 -1.6 1,599,585 - BBR_RS17045 0.85 +0.7 1,599,613 - BBR_RS17045 0.90 -2.0 1,599,729 + +0.3 1,599,732 - -0.2 1,599,749 + -0.4 1,599,757 - +1.5 1,599,767 - -1.3 1,599,768 + +0.2 1,599,768 + +1.0 1,599,776 - -0.1 1,599,822 - +0.0 1,599,865 + -0.4 1,599,955 - BBR_RS17050 0.17 +0.2 1,599,958 + BBR_RS17050 0.18 -0.1 1,599,958 + BBR_RS17050 0.18 -0.1 1,599,958 + BBR_RS17050 0.18 +0.4 1,599,973 + BBR_RS17050 0.19 +0.4 1,599,980 + BBR_RS17050 0.20 +1.0 1,599,988 - BBR_RS17050 0.20 -0.1 1,599,988 - BBR_RS17050 0.20 +0.3 1,599,988 - BBR_RS17050 0.20 +0.0 1,600,008 + BBR_RS17050 0.22 +0.2 1,600,020 + BBR_RS17050 0.23 -0.2 1,600,035 - BBR_RS17050 0.24 +0.1 1,600,048 - BBR_RS17050 0.25 +0.2
Or see this region's nucleotide sequence