Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16995 and BBR_RS17000 are separated by 184 nucleotides BBR_RS17000 and BBR_RS17005 are separated by 123 nucleotides BBR_RS17005 and BBR_RS17010 overlap by 11 nucleotides
BBR_RS16995: BBR_RS16995 - L-lactate dehydrogenase, at 1,584,876 to 1,585,838
_RS16995
BBR_RS17000: BBR_RS17000 - GTPase HflX, at 1,586,023 to 1,587,528
_RS17000
BBR_RS17005: BBR_RS17005 - methyltransferase domain-containing protein, at 1,587,652 to 1,588,308
_RS17005
BBR_RS17010: BBR_RS17010 - DUF3418 domain-containing protein, at 1,588,298 to 1,592,434
_RS17010
Position (kb)
1586
1587
1588 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1585.970 kb on - strand at 1586.152 kb on - strand at 1586.159 kb on + strand at 1586.167 kb on - strand at 1586.179 kb on + strand, within BBR_RS17000 at 1586.179 kb on + strand, within BBR_RS17000 at 1586.181 kb on - strand, within BBR_RS17000 at 1586.187 kb on - strand, within BBR_RS17000 at 1586.200 kb on + strand, within BBR_RS17000 at 1586.216 kb on - strand, within BBR_RS17000 at 1586.229 kb on - strand, within BBR_RS17000 at 1586.237 kb on - strand, within BBR_RS17000 at 1586.321 kb on + strand, within BBR_RS17000 at 1586.329 kb on - strand, within BBR_RS17000 at 1586.346 kb on + strand, within BBR_RS17000 at 1586.354 kb on - strand, within BBR_RS17000 at 1586.359 kb on + strand, within BBR_RS17000 at 1586.381 kb on + strand, within BBR_RS17000 at 1586.432 kb on - strand, within BBR_RS17000 at 1586.459 kb on + strand, within BBR_RS17000 at 1586.491 kb on - strand, within BBR_RS17000 at 1586.569 kb on + strand, within BBR_RS17000 at 1586.584 kb on - strand, within BBR_RS17000 at 1586.621 kb on - strand, within BBR_RS17000 at 1586.624 kb on - strand, within BBR_RS17000 at 1586.706 kb on - strand, within BBR_RS17000 at 1586.764 kb on + strand, within BBR_RS17000 at 1586.789 kb on - strand, within BBR_RS17000 at 1586.809 kb on - strand, within BBR_RS17000 at 1586.811 kb on + strand, within BBR_RS17000 at 1586.811 kb on + strand, within BBR_RS17000 at 1586.819 kb on - strand, within BBR_RS17000 at 1586.819 kb on - strand, within BBR_RS17000 at 1586.819 kb on - strand, within BBR_RS17000 at 1586.863 kb on + strand, within BBR_RS17000 at 1586.872 kb on + strand, within BBR_RS17000 at 1586.880 kb on - strand, within BBR_RS17000 at 1586.911 kb on - strand, within BBR_RS17000 at 1586.922 kb on + strand, within BBR_RS17000 at 1586.922 kb on + strand, within BBR_RS17000 at 1586.930 kb on - strand, within BBR_RS17000 at 1586.933 kb on + strand, within BBR_RS17000 at 1586.933 kb on + strand, within BBR_RS17000 at 1587.013 kb on - strand, within BBR_RS17000 at 1587.038 kb on + strand, within BBR_RS17000 at 1587.088 kb on - strand, within BBR_RS17000 at 1587.138 kb on - strand, within BBR_RS17000 at 1587.149 kb on + strand, within BBR_RS17000 at 1587.150 kb on + strand, within BBR_RS17000 at 1587.166 kb on + strand, within BBR_RS17000 at 1587.181 kb on + strand, within BBR_RS17000 at 1587.183 kb on + strand, within BBR_RS17000 at 1587.229 kb on + strand, within BBR_RS17000 at 1587.236 kb on + strand, within BBR_RS17000 at 1587.244 kb on - strand, within BBR_RS17000 at 1587.244 kb on - strand, within BBR_RS17000 at 1587.244 kb on - strand, within BBR_RS17000 at 1587.272 kb on - strand, within BBR_RS17000 at 1587.273 kb on - strand, within BBR_RS17000 at 1587.276 kb on - strand, within BBR_RS17000 at 1587.282 kb on - strand, within BBR_RS17000 at 1587.320 kb on + strand, within BBR_RS17000 at 1587.439 kb on + strand at 1587.452 kb on - strand at 1587.475 kb on + strand at 1587.524 kb on + strand at 1587.524 kb on + strand at 1587.524 kb on + strand at 1587.532 kb on - strand at 1587.648 kb on + strand at 1587.684 kb on + strand at 1587.704 kb on + strand at 1587.712 kb on - strand at 1587.718 kb on - strand, within BBR_RS17005 at 1587.718 kb on - strand, within BBR_RS17005 at 1587.724 kb on + strand, within BBR_RS17005 at 1587.735 kb on - strand, within BBR_RS17005 at 1587.788 kb on + strand, within BBR_RS17005 at 1587.788 kb on + strand, within BBR_RS17005 at 1587.835 kb on + strand, within BBR_RS17005 at 1587.854 kb on - strand, within BBR_RS17005 at 1587.859 kb on + strand, within BBR_RS17005 at 1587.892 kb on + strand, within BBR_RS17005 at 1587.903 kb on + strand, within BBR_RS17005 at 1587.911 kb on - strand, within BBR_RS17005 at 1587.918 kb on - strand, within BBR_RS17005 at 1587.932 kb on + strand, within BBR_RS17005 at 1587.954 kb on + strand, within BBR_RS17005 at 1587.954 kb on + strand, within BBR_RS17005 at 1587.987 kb on - strand, within BBR_RS17005 at 1587.994 kb on - strand, within BBR_RS17005 at 1588.122 kb on - strand, within BBR_RS17005 at 1588.126 kb on - strand, within BBR_RS17005 at 1588.128 kb on + strand, within BBR_RS17005 at 1588.128 kb on + strand, within BBR_RS17005 at 1588.133 kb on + strand, within BBR_RS17005 at 1588.136 kb on - strand, within BBR_RS17005 at 1588.136 kb on - strand, within BBR_RS17005 at 1588.149 kb on + strand, within BBR_RS17005 at 1588.220 kb on - strand, within BBR_RS17005 at 1588.274 kb on - strand at 1588.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,585,970 - -0.2 1,586,152 - -1.0 1,586,159 + -0.3 1,586,167 - -0.8 1,586,179 + BBR_RS17000 0.10 +0.1 1,586,179 + BBR_RS17000 0.10 +0.1 1,586,181 - BBR_RS17000 0.10 -0.2 1,586,187 - BBR_RS17000 0.11 +0.6 1,586,200 + BBR_RS17000 0.12 +1.1 1,586,216 - BBR_RS17000 0.13 +0.5 1,586,229 - BBR_RS17000 0.14 +0.2 1,586,237 - BBR_RS17000 0.14 +1.0 1,586,321 + BBR_RS17000 0.20 +0.9 1,586,329 - BBR_RS17000 0.20 +0.4 1,586,346 + BBR_RS17000 0.21 +0.9 1,586,354 - BBR_RS17000 0.22 +0.6 1,586,359 + BBR_RS17000 0.22 +0.0 1,586,381 + BBR_RS17000 0.24 +0.1 1,586,432 - BBR_RS17000 0.27 +0.8 1,586,459 + BBR_RS17000 0.29 -0.6 1,586,491 - BBR_RS17000 0.31 +0.4 1,586,569 + BBR_RS17000 0.36 +1.7 1,586,584 - BBR_RS17000 0.37 +0.8 1,586,621 - BBR_RS17000 0.40 -1.2 1,586,624 - BBR_RS17000 0.40 +0.2 1,586,706 - BBR_RS17000 0.45 -0.3 1,586,764 + BBR_RS17000 0.49 -0.0 1,586,789 - BBR_RS17000 0.51 +0.3 1,586,809 - BBR_RS17000 0.52 +0.8 1,586,811 + BBR_RS17000 0.52 -0.2 1,586,811 + BBR_RS17000 0.52 -0.7 1,586,819 - BBR_RS17000 0.53 -0.0 1,586,819 - BBR_RS17000 0.53 +0.4 1,586,819 - BBR_RS17000 0.53 -0.1 1,586,863 + BBR_RS17000 0.56 -1.1 1,586,872 + BBR_RS17000 0.56 +0.2 1,586,880 - BBR_RS17000 0.57 +0.5 1,586,911 - BBR_RS17000 0.59 +0.8 1,586,922 + BBR_RS17000 0.60 -0.1 1,586,922 + BBR_RS17000 0.60 -0.2 1,586,930 - BBR_RS17000 0.60 -0.6 1,586,933 + BBR_RS17000 0.60 +0.4 1,586,933 + BBR_RS17000 0.60 -0.1 1,587,013 - BBR_RS17000 0.66 +0.9 1,587,038 + BBR_RS17000 0.67 +0.9 1,587,088 - BBR_RS17000 0.71 -0.2 1,587,138 - BBR_RS17000 0.74 +1.1 1,587,149 + BBR_RS17000 0.75 -0.1 1,587,150 + BBR_RS17000 0.75 +1.9 1,587,166 + BBR_RS17000 0.76 -0.6 1,587,181 + BBR_RS17000 0.77 -0.2 1,587,183 + BBR_RS17000 0.77 +0.7 1,587,229 + BBR_RS17000 0.80 +0.2 1,587,236 + BBR_RS17000 0.81 +0.7 1,587,244 - BBR_RS17000 0.81 -0.2 1,587,244 - BBR_RS17000 0.81 +0.7 1,587,244 - BBR_RS17000 0.81 -0.2 1,587,272 - BBR_RS17000 0.83 +0.3 1,587,273 - BBR_RS17000 0.83 +0.7 1,587,276 - BBR_RS17000 0.83 +0.2 1,587,282 - BBR_RS17000 0.84 +0.3 1,587,320 + BBR_RS17000 0.86 +0.5 1,587,439 + +0.8 1,587,452 - +0.1 1,587,475 + -0.5 1,587,524 + -0.2 1,587,524 + -1.1 1,587,524 + -1.0 1,587,532 - -1.5 1,587,648 + +0.3 1,587,684 + +0.2 1,587,704 + +0.2 1,587,712 - +0.4 1,587,718 - BBR_RS17005 0.10 -0.4 1,587,718 - BBR_RS17005 0.10 +0.9 1,587,724 + BBR_RS17005 0.11 +0.5 1,587,735 - BBR_RS17005 0.13 +0.4 1,587,788 + BBR_RS17005 0.21 -1.0 1,587,788 + BBR_RS17005 0.21 -0.3 1,587,835 + BBR_RS17005 0.28 +0.3 1,587,854 - BBR_RS17005 0.31 +1.7 1,587,859 + BBR_RS17005 0.32 +0.6 1,587,892 + BBR_RS17005 0.37 +0.4 1,587,903 + BBR_RS17005 0.38 -0.2 1,587,911 - BBR_RS17005 0.39 -0.4 1,587,918 - BBR_RS17005 0.40 -0.1 1,587,932 + BBR_RS17005 0.43 +0.2 1,587,954 + BBR_RS17005 0.46 -0.0 1,587,954 + BBR_RS17005 0.46 -0.4 1,587,987 - BBR_RS17005 0.51 -0.2 1,587,994 - BBR_RS17005 0.52 +0.3 1,588,122 - BBR_RS17005 0.72 +0.8 1,588,126 - BBR_RS17005 0.72 -0.1 1,588,128 + BBR_RS17005 0.72 +0.7 1,588,128 + BBR_RS17005 0.72 +0.7 1,588,133 + BBR_RS17005 0.73 +0.8 1,588,136 - BBR_RS17005 0.74 +0.5 1,588,136 - BBR_RS17005 0.74 -0.1 1,588,149 + BBR_RS17005 0.76 +0.4 1,588,220 - BBR_RS17005 0.86 -0.4 1,588,274 - -2.0 1,588,391 + -0.2
Or see this region's nucleotide sequence