Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16850 and BBR_RS16855 are separated by 137 nucleotides BBR_RS16855 and BBR_RS16860 are separated by 97 nucleotides BBR_RS16860 and BBR_RS16865 are separated by 54 nucleotides
BBR_RS16850: BBR_RS16850 - peptidase M24 family protein, at 1,552,234 to 1,553,829
_RS16850
BBR_RS16855: BBR_RS16855 - NUDIX domain-containing protein, at 1,553,967 to 1,554,488
_RS16855
BBR_RS16860: BBR_RS16860 - N-acetylglucosamine-6-phosphate deacetylase, at 1,554,586 to 1,555,863
_RS16860
BBR_RS16865: BBR_RS16865 - glucosamine-6-phosphate deaminase, at 1,555,918 to 1,556,730
_RS16865
Position (kb)
1554
1555
1556 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1553.586 kb on + strand, within BBR_RS16850 at 1553.600 kb on + strand, within BBR_RS16850 at 1553.655 kb on + strand, within BBR_RS16850 at 1553.658 kb on - strand, within BBR_RS16850 at 1553.707 kb on - strand at 1553.735 kb on - strand at 1553.804 kb on - strand at 1553.809 kb on - strand at 1553.809 kb on - strand at 1553.859 kb on - strand at 1553.875 kb on + strand at 1553.881 kb on + strand at 1553.896 kb on + strand at 1553.899 kb on + strand at 1554.010 kb on + strand at 1554.032 kb on - strand, within BBR_RS16855 at 1554.143 kb on + strand, within BBR_RS16855 at 1554.325 kb on - strand, within BBR_RS16855 at 1554.337 kb on + strand, within BBR_RS16855 at 1554.337 kb on + strand, within BBR_RS16855 at 1554.337 kb on + strand, within BBR_RS16855 at 1554.345 kb on - strand, within BBR_RS16855 at 1554.360 kb on - strand, within BBR_RS16855 at 1554.365 kb on - strand, within BBR_RS16855 at 1554.386 kb on + strand, within BBR_RS16855 at 1554.411 kb on - strand, within BBR_RS16855 at 1554.461 kb on + strand at 1554.539 kb on + strand at 1554.566 kb on - strand at 1554.588 kb on + strand at 1554.600 kb on + strand at 1554.608 kb on + strand at 1554.608 kb on + strand at 1554.608 kb on + strand at 1554.608 kb on + strand at 1554.616 kb on - strand at 1554.616 kb on - strand at 1554.623 kb on + strand at 1554.638 kb on + strand at 1554.728 kb on + strand, within BBR_RS16860 at 1554.760 kb on - strand, within BBR_RS16860 at 1554.775 kb on + strand, within BBR_RS16860 at 1554.807 kb on - strand, within BBR_RS16860 at 1554.943 kb on + strand, within BBR_RS16860 at 1555.021 kb on - strand, within BBR_RS16860 at 1555.023 kb on + strand, within BBR_RS16860 at 1555.023 kb on + strand, within BBR_RS16860 at 1555.023 kb on + strand, within BBR_RS16860 at 1555.044 kb on + strand, within BBR_RS16860 at 1555.088 kb on - strand, within BBR_RS16860 at 1555.105 kb on - strand, within BBR_RS16860 at 1555.199 kb on + strand, within BBR_RS16860 at 1555.236 kb on + strand, within BBR_RS16860 at 1555.265 kb on - strand, within BBR_RS16860 at 1555.267 kb on + strand, within BBR_RS16860 at 1555.273 kb on - strand, within BBR_RS16860 at 1555.284 kb on - strand, within BBR_RS16860 at 1555.325 kb on + strand, within BBR_RS16860 at 1555.325 kb on + strand, within BBR_RS16860 at 1555.332 kb on + strand, within BBR_RS16860 at 1555.358 kb on + strand, within BBR_RS16860 at 1555.366 kb on + strand, within BBR_RS16860 at 1555.406 kb on + strand, within BBR_RS16860 at 1555.531 kb on + strand, within BBR_RS16860 at 1555.536 kb on + strand, within BBR_RS16860 at 1555.572 kb on + strand, within BBR_RS16860 at 1555.580 kb on - strand, within BBR_RS16860 at 1555.580 kb on - strand, within BBR_RS16860 at 1555.610 kb on - strand, within BBR_RS16860 at 1555.628 kb on + strand, within BBR_RS16860 at 1555.628 kb on + strand, within BBR_RS16860 at 1555.638 kb on + strand, within BBR_RS16860 at 1555.640 kb on + strand, within BBR_RS16860 at 1555.640 kb on + strand, within BBR_RS16860 at 1555.646 kb on - strand, within BBR_RS16860 at 1555.646 kb on - strand, within BBR_RS16860 at 1555.648 kb on - strand, within BBR_RS16860 at 1555.648 kb on - strand, within BBR_RS16860 at 1555.673 kb on + strand, within BBR_RS16860 at 1555.673 kb on + strand, within BBR_RS16860 at 1555.681 kb on - strand, within BBR_RS16860 at 1555.681 kb on - strand, within BBR_RS16860 at 1555.691 kb on - strand, within BBR_RS16860 at 1555.732 kb on + strand, within BBR_RS16860 at 1555.750 kb on - strand at 1555.750 kb on - strand at 1555.778 kb on - strand at 1555.838 kb on - strand at 1555.875 kb on + strand at 1555.875 kb on + strand at 1555.890 kb on + strand at 1555.898 kb on + strand at 1555.911 kb on + strand at 1555.917 kb on - strand at 1555.960 kb on + strand at 1555.969 kb on - strand at 1555.982 kb on - strand at 1555.991 kb on + strand at 1555.992 kb on - strand at 1556.016 kb on + strand, within BBR_RS16865 at 1556.034 kb on + strand, within BBR_RS16865 at 1556.087 kb on - strand, within BBR_RS16865 at 1556.092 kb on + strand, within BBR_RS16865 at 1556.112 kb on - strand, within BBR_RS16865 at 1556.157 kb on + strand, within BBR_RS16865 at 1556.165 kb on - strand, within BBR_RS16865 at 1556.165 kb on - strand, within BBR_RS16865 at 1556.165 kb on - strand, within BBR_RS16865 at 1556.165 kb on - strand, within BBR_RS16865 at 1556.171 kb on + strand, within BBR_RS16865 at 1556.171 kb on + strand, within BBR_RS16865 at 1556.188 kb on - strand, within BBR_RS16865 at 1556.189 kb on - strand, within BBR_RS16865 at 1556.189 kb on - strand, within BBR_RS16865 at 1556.221 kb on + strand, within BBR_RS16865 at 1556.221 kb on + strand, within BBR_RS16865 at 1556.245 kb on - strand, within BBR_RS16865 at 1556.259 kb on + strand, within BBR_RS16865 at 1556.282 kb on - strand, within BBR_RS16865 at 1556.353 kb on + strand, within BBR_RS16865 at 1556.353 kb on + strand, within BBR_RS16865 at 1556.353 kb on + strand, within BBR_RS16865 at 1556.361 kb on - strand, within BBR_RS16865 at 1556.361 kb on - strand, within BBR_RS16865 at 1556.442 kb on + strand, within BBR_RS16865 at 1556.450 kb on - strand, within BBR_RS16865 at 1556.450 kb on - strand, within BBR_RS16865 at 1556.456 kb on - strand, within BBR_RS16865 at 1556.464 kb on - strand, within BBR_RS16865 at 1556.466 kb on + strand, within BBR_RS16865 at 1556.471 kb on - strand, within BBR_RS16865 at 1556.493 kb on + strand, within BBR_RS16865 at 1556.506 kb on - strand, within BBR_RS16865 at 1556.506 kb on - strand, within BBR_RS16865 at 1556.555 kb on - strand, within BBR_RS16865 at 1556.587 kb on - strand, within BBR_RS16865 at 1556.618 kb on - strand, within BBR_RS16865 at 1556.619 kb on - strand, within BBR_RS16865 at 1556.629 kb on + strand, within BBR_RS16865 at 1556.631 kb on - strand, within BBR_RS16865 at 1556.637 kb on - strand, within BBR_RS16865 at 1556.637 kb on - strand, within BBR_RS16865 at 1556.775 kb on + strand at 1556.803 kb on - strand at 1556.828 kb on + strand at 1556.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,553,586 + BBR_RS16850 0.85 +0.3 1,553,600 + BBR_RS16850 0.86 +0.6 1,553,655 + BBR_RS16850 0.89 -0.3 1,553,658 - BBR_RS16850 0.89 +0.9 1,553,707 - -0.6 1,553,735 - -0.8 1,553,804 - +0.3 1,553,809 - +0.6 1,553,809 - +0.2 1,553,859 - +0.5 1,553,875 + -0.1 1,553,881 + -0.6 1,553,896 + +0.3 1,553,899 + -0.0 1,554,010 + +0.5 1,554,032 - BBR_RS16855 0.12 -0.1 1,554,143 + BBR_RS16855 0.34 -0.9 1,554,325 - BBR_RS16855 0.69 -1.0 1,554,337 + BBR_RS16855 0.71 -0.0 1,554,337 + BBR_RS16855 0.71 -0.3 1,554,337 + BBR_RS16855 0.71 +0.3 1,554,345 - BBR_RS16855 0.72 -0.8 1,554,360 - BBR_RS16855 0.75 +1.1 1,554,365 - BBR_RS16855 0.76 +1.3 1,554,386 + BBR_RS16855 0.80 +0.3 1,554,411 - BBR_RS16855 0.85 +1.2 1,554,461 + +0.3 1,554,539 + +0.3 1,554,566 - -0.4 1,554,588 + -0.3 1,554,600 + -0.4 1,554,608 + -0.6 1,554,608 + +0.7 1,554,608 + +0.4 1,554,608 + +0.1 1,554,616 - +0.8 1,554,616 - +0.4 1,554,623 + -0.2 1,554,638 + -0.9 1,554,728 + BBR_RS16860 0.11 +0.7 1,554,760 - BBR_RS16860 0.14 +0.4 1,554,775 + BBR_RS16860 0.15 +0.3 1,554,807 - BBR_RS16860 0.17 +0.5 1,554,943 + BBR_RS16860 0.28 +0.1 1,555,021 - BBR_RS16860 0.34 +0.5 1,555,023 + BBR_RS16860 0.34 -0.5 1,555,023 + BBR_RS16860 0.34 +0.5 1,555,023 + BBR_RS16860 0.34 +0.6 1,555,044 + BBR_RS16860 0.36 +0.5 1,555,088 - BBR_RS16860 0.39 +0.6 1,555,105 - BBR_RS16860 0.41 +0.6 1,555,199 + BBR_RS16860 0.48 -0.0 1,555,236 + BBR_RS16860 0.51 +0.4 1,555,265 - BBR_RS16860 0.53 +0.8 1,555,267 + BBR_RS16860 0.53 -0.3 1,555,273 - BBR_RS16860 0.54 -0.0 1,555,284 - BBR_RS16860 0.55 -0.9 1,555,325 + BBR_RS16860 0.58 +0.3 1,555,325 + BBR_RS16860 0.58 +0.2 1,555,332 + BBR_RS16860 0.58 +1.0 1,555,358 + BBR_RS16860 0.60 -1.4 1,555,366 + BBR_RS16860 0.61 +0.4 1,555,406 + BBR_RS16860 0.64 +1.0 1,555,531 + BBR_RS16860 0.74 +0.0 1,555,536 + BBR_RS16860 0.74 -0.6 1,555,572 + BBR_RS16860 0.77 +0.4 1,555,580 - BBR_RS16860 0.78 -0.1 1,555,580 - BBR_RS16860 0.78 -0.1 1,555,610 - BBR_RS16860 0.80 -0.3 1,555,628 + BBR_RS16860 0.82 +0.4 1,555,628 + BBR_RS16860 0.82 +0.4 1,555,638 + BBR_RS16860 0.82 +0.1 1,555,640 + BBR_RS16860 0.82 +0.2 1,555,640 + BBR_RS16860 0.82 +0.3 1,555,646 - BBR_RS16860 0.83 -0.2 1,555,646 - BBR_RS16860 0.83 +0.1 1,555,648 - BBR_RS16860 0.83 -0.4 1,555,648 - BBR_RS16860 0.83 -0.1 1,555,673 + BBR_RS16860 0.85 -0.6 1,555,673 + BBR_RS16860 0.85 -0.6 1,555,681 - BBR_RS16860 0.86 +1.1 1,555,681 - BBR_RS16860 0.86 +0.8 1,555,691 - BBR_RS16860 0.86 -0.6 1,555,732 + BBR_RS16860 0.90 -0.3 1,555,750 - +0.2 1,555,750 - +0.1 1,555,778 - -0.2 1,555,838 - +0.5 1,555,875 + -0.4 1,555,875 + +0.2 1,555,890 + -0.1 1,555,898 + +0.4 1,555,911 + -0.0 1,555,917 - -0.1 1,555,960 + +0.5 1,555,969 - -0.4 1,555,982 - +0.5 1,555,991 + +0.6 1,555,992 - +0.3 1,556,016 + BBR_RS16865 0.12 -0.1 1,556,034 + BBR_RS16865 0.14 -1.9 1,556,087 - BBR_RS16865 0.21 -2.9 1,556,092 + BBR_RS16865 0.21 -0.0 1,556,112 - BBR_RS16865 0.24 +0.0 1,556,157 + BBR_RS16865 0.29 +0.4 1,556,165 - BBR_RS16865 0.30 +0.2 1,556,165 - BBR_RS16865 0.30 -0.5 1,556,165 - BBR_RS16865 0.30 +0.4 1,556,165 - BBR_RS16865 0.30 -1.9 1,556,171 + BBR_RS16865 0.31 +1.1 1,556,171 + BBR_RS16865 0.31 -0.2 1,556,188 - BBR_RS16865 0.33 +0.3 1,556,189 - BBR_RS16865 0.33 +0.3 1,556,189 - BBR_RS16865 0.33 +0.0 1,556,221 + BBR_RS16865 0.37 -0.3 1,556,221 + BBR_RS16865 0.37 -0.1 1,556,245 - BBR_RS16865 0.40 -0.1 1,556,259 + BBR_RS16865 0.42 -0.0 1,556,282 - BBR_RS16865 0.45 +0.8 1,556,353 + BBR_RS16865 0.54 +0.2 1,556,353 + BBR_RS16865 0.54 -0.0 1,556,353 + BBR_RS16865 0.54 +0.8 1,556,361 - BBR_RS16865 0.54 -0.6 1,556,361 - BBR_RS16865 0.54 +0.0 1,556,442 + BBR_RS16865 0.64 +0.1 1,556,450 - BBR_RS16865 0.65 -0.3 1,556,450 - BBR_RS16865 0.65 +0.2 1,556,456 - BBR_RS16865 0.66 +0.0 1,556,464 - BBR_RS16865 0.67 +0.3 1,556,466 + BBR_RS16865 0.67 -0.1 1,556,471 - BBR_RS16865 0.68 -0.2 1,556,493 + BBR_RS16865 0.71 -0.4 1,556,506 - BBR_RS16865 0.72 +0.7 1,556,506 - BBR_RS16865 0.72 -0.2 1,556,555 - BBR_RS16865 0.78 +0.9 1,556,587 - BBR_RS16865 0.82 -0.8 1,556,618 - BBR_RS16865 0.86 -0.0 1,556,619 - BBR_RS16865 0.86 -0.5 1,556,629 + BBR_RS16865 0.87 -0.1 1,556,631 - BBR_RS16865 0.88 +0.2 1,556,637 - BBR_RS16865 0.88 -0.1 1,556,637 - BBR_RS16865 0.88 +0.4 1,556,775 + +0.8 1,556,803 - -2.0 1,556,828 + -0.8 1,556,837 + -0.1
Or see this region's nucleotide sequence