Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16705 and BBR_RS16710 are separated by 104 nucleotides BBR_RS16710 and BBR_RS16715 are separated by 97 nucleotides
BBR_RS16705: BBR_RS16705 - ABC transporter permease, at 1,518,701 to 1,521,823
_RS16705
BBR_RS16710: BBR_RS16710 - hypothetical protein, at 1,521,928 to 1,522,152
_RS16710
BBR_RS16715: BBR_RS16715 - cysteine desulfurase, at 1,522,250 to 1,523,548
_RS16715
Position (kb)
1521
1522
1523 Strain fitness (log2 ratio)
-1
0
1
2 at 1520.930 kb on + strand, within BBR_RS16705 at 1520.943 kb on - strand, within BBR_RS16705 at 1520.963 kb on - strand, within BBR_RS16705 at 1520.972 kb on - strand, within BBR_RS16705 at 1521.064 kb on + strand, within BBR_RS16705 at 1521.086 kb on + strand, within BBR_RS16705 at 1521.143 kb on + strand, within BBR_RS16705 at 1521.143 kb on + strand, within BBR_RS16705 at 1521.151 kb on - strand, within BBR_RS16705 at 1521.159 kb on - strand, within BBR_RS16705 at 1521.180 kb on + strand, within BBR_RS16705 at 1521.237 kb on + strand, within BBR_RS16705 at 1521.245 kb on - strand, within BBR_RS16705 at 1521.267 kb on + strand, within BBR_RS16705 at 1521.267 kb on + strand, within BBR_RS16705 at 1521.275 kb on - strand, within BBR_RS16705 at 1521.308 kb on - strand, within BBR_RS16705 at 1521.326 kb on + strand, within BBR_RS16705 at 1521.345 kb on + strand, within BBR_RS16705 at 1521.355 kb on + strand, within BBR_RS16705 at 1521.394 kb on + strand, within BBR_RS16705 at 1521.522 kb on - strand at 1521.522 kb on - strand at 1521.526 kb on - strand at 1521.580 kb on - strand at 1521.583 kb on + strand at 1521.587 kb on - strand at 1521.602 kb on + strand at 1521.690 kb on + strand at 1521.697 kb on + strand at 1521.698 kb on - strand at 1521.712 kb on + strand at 1521.712 kb on + strand at 1521.720 kb on - strand at 1521.744 kb on - strand at 1521.752 kb on - strand at 1521.752 kb on - strand at 1521.769 kb on - strand at 1521.778 kb on + strand at 1521.789 kb on - strand at 1521.821 kb on - strand at 1521.825 kb on - strand at 1521.843 kb on - strand at 1521.887 kb on + strand at 1521.892 kb on + strand at 1521.895 kb on - strand at 1521.914 kb on + strand at 1521.940 kb on + strand at 1521.959 kb on + strand, within BBR_RS16710 at 1521.964 kb on + strand, within BBR_RS16710 at 1521.972 kb on - strand, within BBR_RS16710 at 1521.977 kb on - strand, within BBR_RS16710 at 1521.980 kb on - strand, within BBR_RS16710 at 1521.995 kb on - strand, within BBR_RS16710 at 1522.042 kb on + strand, within BBR_RS16710 at 1522.045 kb on - strand, within BBR_RS16710 at 1522.213 kb on - strand at 1522.228 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.269 kb on + strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.277 kb on - strand at 1522.287 kb on - strand at 1522.316 kb on + strand at 1522.316 kb on + strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.324 kb on - strand at 1522.326 kb on + strand at 1522.326 kb on + strand at 1522.326 kb on + strand at 1522.335 kb on + strand at 1522.343 kb on - strand at 1522.406 kb on + strand, within BBR_RS16715 at 1522.421 kb on + strand, within BBR_RS16715 at 1522.421 kb on + strand, within BBR_RS16715 at 1522.438 kb on - strand, within BBR_RS16715 at 1522.580 kb on - strand, within BBR_RS16715 at 1522.660 kb on + strand, within BBR_RS16715 at 1522.660 kb on + strand, within BBR_RS16715 at 1522.668 kb on - strand, within BBR_RS16715 at 1522.671 kb on + strand, within BBR_RS16715 at 1522.671 kb on + strand, within BBR_RS16715 at 1522.683 kb on + strand, within BBR_RS16715 at 1522.686 kb on + strand, within BBR_RS16715 at 1522.704 kb on - strand, within BBR_RS16715 at 1522.704 kb on - strand, within BBR_RS16715 at 1522.711 kb on - strand, within BBR_RS16715 at 1522.728 kb on + strand, within BBR_RS16715 at 1522.728 kb on + strand, within BBR_RS16715 at 1522.736 kb on - strand, within BBR_RS16715 at 1522.751 kb on + strand, within BBR_RS16715 at 1522.757 kb on - strand, within BBR_RS16715 at 1522.757 kb on - strand, within BBR_RS16715 at 1522.757 kb on - strand, within BBR_RS16715 at 1522.761 kb on + strand, within BBR_RS16715 at 1522.761 kb on - strand, within BBR_RS16715 at 1522.777 kb on - strand, within BBR_RS16715 at 1522.777 kb on - strand, within BBR_RS16715 at 1522.815 kb on - strand, within BBR_RS16715 at 1522.873 kb on - strand, within BBR_RS16715 at 1522.888 kb on + strand, within BBR_RS16715 at 1522.889 kb on + strand, within BBR_RS16715 at 1522.912 kb on + strand, within BBR_RS16715 at 1522.956 kb on + strand, within BBR_RS16715 at 1522.973 kb on + strand, within BBR_RS16715 at 1523.103 kb on + strand, within BBR_RS16715 at 1523.104 kb on - strand, within BBR_RS16715 at 1523.124 kb on + strand, within BBR_RS16715
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,520,930 + BBR_RS16705 0.71 -1.2 1,520,943 - BBR_RS16705 0.72 -0.4 1,520,963 - BBR_RS16705 0.72 +0.6 1,520,972 - BBR_RS16705 0.73 +0.2 1,521,064 + BBR_RS16705 0.76 -0.3 1,521,086 + BBR_RS16705 0.76 -0.5 1,521,143 + BBR_RS16705 0.78 -1.0 1,521,143 + BBR_RS16705 0.78 -0.3 1,521,151 - BBR_RS16705 0.78 +0.1 1,521,159 - BBR_RS16705 0.79 -0.6 1,521,180 + BBR_RS16705 0.79 +0.1 1,521,237 + BBR_RS16705 0.81 +0.1 1,521,245 - BBR_RS16705 0.81 +1.0 1,521,267 + BBR_RS16705 0.82 -0.1 1,521,267 + BBR_RS16705 0.82 -0.0 1,521,275 - BBR_RS16705 0.82 -0.2 1,521,308 - BBR_RS16705 0.83 +0.5 1,521,326 + BBR_RS16705 0.84 -0.3 1,521,345 + BBR_RS16705 0.85 +1.5 1,521,355 + BBR_RS16705 0.85 +0.4 1,521,394 + BBR_RS16705 0.86 -0.4 1,521,522 - -0.8 1,521,522 - -1.6 1,521,526 - -1.3 1,521,580 - -0.3 1,521,583 + -0.1 1,521,587 - +0.7 1,521,602 + +0.8 1,521,690 + +0.4 1,521,697 + +0.4 1,521,698 - -0.2 1,521,712 + +0.6 1,521,712 + -0.4 1,521,720 - -1.0 1,521,744 - +1.1 1,521,752 - +0.5 1,521,752 - +0.8 1,521,769 - +0.5 1,521,778 + +0.5 1,521,789 - +0.1 1,521,821 - +0.0 1,521,825 - +0.1 1,521,843 - +1.3 1,521,887 + +0.4 1,521,892 + +0.2 1,521,895 - +0.1 1,521,914 + -0.1 1,521,940 + -0.4 1,521,959 + BBR_RS16710 0.14 +0.6 1,521,964 + BBR_RS16710 0.16 +0.0 1,521,972 - BBR_RS16710 0.20 -0.1 1,521,977 - BBR_RS16710 0.22 +0.1 1,521,980 - BBR_RS16710 0.23 -0.2 1,521,995 - BBR_RS16710 0.30 +0.8 1,522,042 + BBR_RS16710 0.51 +0.6 1,522,045 - BBR_RS16710 0.52 +0.3 1,522,213 - -0.0 1,522,228 + +0.8 1,522,269 + +0.8 1,522,269 + -0.0 1,522,269 + -0.9 1,522,269 + +0.9 1,522,269 + +0.2 1,522,269 + -0.2 1,522,269 + +0.3 1,522,269 + -0.6 1,522,269 + +0.2 1,522,269 + +1.8 1,522,269 + +0.9 1,522,269 + +0.1 1,522,269 + -0.5 1,522,269 + -0.0 1,522,277 - +0.2 1,522,277 - -0.9 1,522,277 - -0.0 1,522,277 - -0.5 1,522,277 - +0.6 1,522,277 - +0.4 1,522,277 - +0.3 1,522,277 - +1.0 1,522,277 - +0.4 1,522,287 - -0.4 1,522,316 + -0.2 1,522,316 + +0.2 1,522,324 - +0.1 1,522,324 - +0.1 1,522,324 - -0.7 1,522,324 - +0.1 1,522,324 - +0.4 1,522,324 - -0.6 1,522,324 - -0.4 1,522,324 - -0.3 1,522,326 + -0.6 1,522,326 + +0.3 1,522,326 + -0.3 1,522,335 + -0.5 1,522,343 - -0.1 1,522,406 + BBR_RS16715 0.12 +0.2 1,522,421 + BBR_RS16715 0.13 +0.7 1,522,421 + BBR_RS16715 0.13 +0.2 1,522,438 - BBR_RS16715 0.14 -0.1 1,522,580 - BBR_RS16715 0.25 +0.2 1,522,660 + BBR_RS16715 0.32 +0.3 1,522,660 + BBR_RS16715 0.32 +0.5 1,522,668 - BBR_RS16715 0.32 -0.2 1,522,671 + BBR_RS16715 0.32 +0.4 1,522,671 + BBR_RS16715 0.32 -0.3 1,522,683 + BBR_RS16715 0.33 -0.7 1,522,686 + BBR_RS16715 0.34 +0.3 1,522,704 - BBR_RS16715 0.35 -0.5 1,522,704 - BBR_RS16715 0.35 -0.4 1,522,711 - BBR_RS16715 0.35 +0.5 1,522,728 + BBR_RS16715 0.37 -1.4 1,522,728 + BBR_RS16715 0.37 -0.7 1,522,736 - BBR_RS16715 0.37 -1.0 1,522,751 + BBR_RS16715 0.39 +1.1 1,522,757 - BBR_RS16715 0.39 -0.2 1,522,757 - BBR_RS16715 0.39 +0.4 1,522,757 - BBR_RS16715 0.39 +0.7 1,522,761 + BBR_RS16715 0.39 +0.9 1,522,761 - BBR_RS16715 0.39 +0.2 1,522,777 - BBR_RS16715 0.41 -0.0 1,522,777 - BBR_RS16715 0.41 -0.2 1,522,815 - BBR_RS16715 0.43 +0.9 1,522,873 - BBR_RS16715 0.48 -0.1 1,522,888 + BBR_RS16715 0.49 +0.0 1,522,889 + BBR_RS16715 0.49 +0.1 1,522,912 + BBR_RS16715 0.51 +1.5 1,522,956 + BBR_RS16715 0.54 +0.3 1,522,973 + BBR_RS16715 0.56 +0.8 1,523,103 + BBR_RS16715 0.66 -0.2 1,523,104 - BBR_RS16715 0.66 -0.3 1,523,124 + BBR_RS16715 0.67 +0.2
Or see this region's nucleotide sequence