Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16340 and BBR_RS16345 are separated by 277 nucleotides BBR_RS16345 and BBR_RS16350 are separated by 13 nucleotides BBR_RS16350 and BBR_RS16355 overlap by 1 nucleotides
BBR_RS16340: BBR_RS16340 - DUF5110 domain-containing protein, at 1,415,729 to 1,418,218
_RS16340
BBR_RS16345: BBR_RS16345 - hypothetical protein, at 1,418,496 to 1,419,785
_RS16345
BBR_RS16350: BBR_RS16350 - carbohydrate ABC transporter permease, at 1,419,799 to 1,420,656
_RS16350
BBR_RS16355: BBR_RS16355 - sugar ABC transporter permease, at 1,420,656 to 1,421,594
_RS16355
Position (kb)
1418
1419
1420 Strain fitness (log2 ratio)
-1
0
1
2 at 1417.503 kb on + strand, within BBR_RS16340 at 1417.545 kb on - strand, within BBR_RS16340 at 1417.557 kb on - strand, within BBR_RS16340 at 1417.579 kb on + strand, within BBR_RS16340 at 1417.584 kb on + strand, within BBR_RS16340 at 1417.629 kb on - strand, within BBR_RS16340 at 1417.678 kb on + strand, within BBR_RS16340 at 1417.690 kb on + strand, within BBR_RS16340 at 1417.713 kb on - strand, within BBR_RS16340 at 1417.715 kb on + strand, within BBR_RS16340 at 1417.797 kb on + strand, within BBR_RS16340 at 1417.832 kb on + strand, within BBR_RS16340 at 1417.833 kb on - strand, within BBR_RS16340 at 1417.833 kb on - strand, within BBR_RS16340 at 1417.850 kb on - strand, within BBR_RS16340 at 1417.884 kb on + strand, within BBR_RS16340 at 1417.892 kb on - strand, within BBR_RS16340 at 1417.925 kb on + strand, within BBR_RS16340 at 1417.926 kb on - strand, within BBR_RS16340 at 1417.926 kb on - strand, within BBR_RS16340 at 1417.933 kb on - strand, within BBR_RS16340 at 1417.933 kb on - strand, within BBR_RS16340 at 1417.967 kb on - strand, within BBR_RS16340 at 1418.011 kb on - strand at 1418.013 kb on + strand at 1418.013 kb on + strand at 1418.013 kb on + strand at 1418.021 kb on - strand at 1418.025 kb on + strand at 1418.109 kb on + strand at 1418.203 kb on + strand at 1418.211 kb on - strand at 1418.214 kb on + strand at 1418.222 kb on - strand at 1418.236 kb on - strand at 1418.329 kb on + strand at 1418.337 kb on - strand at 1418.343 kb on + strand at 1418.351 kb on - strand at 1418.366 kb on + strand at 1418.375 kb on + strand at 1418.431 kb on + strand at 1418.462 kb on + strand at 1418.470 kb on - strand at 1418.470 kb on - strand at 1418.694 kb on + strand, within BBR_RS16345 at 1418.712 kb on - strand, within BBR_RS16345 at 1418.712 kb on - strand, within BBR_RS16345 at 1418.724 kb on + strand, within BBR_RS16345 at 1418.732 kb on - strand, within BBR_RS16345 at 1418.765 kb on + strand, within BBR_RS16345 at 1418.813 kb on - strand, within BBR_RS16345 at 1418.820 kb on + strand, within BBR_RS16345 at 1418.846 kb on + strand, within BBR_RS16345 at 1418.846 kb on + strand, within BBR_RS16345 at 1418.846 kb on + strand, within BBR_RS16345 at 1418.846 kb on + strand, within BBR_RS16345 at 1418.854 kb on - strand, within BBR_RS16345 at 1418.854 kb on - strand, within BBR_RS16345 at 1418.854 kb on - strand, within BBR_RS16345 at 1418.892 kb on + strand, within BBR_RS16345 at 1418.900 kb on - strand, within BBR_RS16345 at 1418.902 kb on + strand, within BBR_RS16345 at 1418.902 kb on + strand, within BBR_RS16345 at 1418.902 kb on + strand, within BBR_RS16345 at 1418.905 kb on - strand, within BBR_RS16345 at 1418.905 kb on - strand, within BBR_RS16345 at 1418.910 kb on - strand, within BBR_RS16345 at 1418.925 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.927 kb on + strand, within BBR_RS16345 at 1418.935 kb on - strand, within BBR_RS16345 at 1418.935 kb on - strand, within BBR_RS16345 at 1419.017 kb on + strand, within BBR_RS16345 at 1419.055 kb on + strand, within BBR_RS16345 at 1419.055 kb on + strand, within BBR_RS16345 at 1419.086 kb on + strand, within BBR_RS16345 at 1419.112 kb on + strand, within BBR_RS16345 at 1419.112 kb on + strand, within BBR_RS16345 at 1419.120 kb on - strand, within BBR_RS16345 at 1419.120 kb on - strand, within BBR_RS16345 at 1419.122 kb on + strand, within BBR_RS16345 at 1419.130 kb on + strand, within BBR_RS16345 at 1419.130 kb on - strand, within BBR_RS16345 at 1419.139 kb on + strand, within BBR_RS16345 at 1419.139 kb on + strand, within BBR_RS16345 at 1419.144 kb on + strand, within BBR_RS16345 at 1419.147 kb on - strand, within BBR_RS16345 at 1419.147 kb on - strand, within BBR_RS16345 at 1419.147 kb on - strand, within BBR_RS16345 at 1419.152 kb on - strand, within BBR_RS16345 at 1419.154 kb on + strand, within BBR_RS16345 at 1419.166 kb on + strand, within BBR_RS16345 at 1419.316 kb on + strand, within BBR_RS16345 at 1419.324 kb on - strand, within BBR_RS16345 at 1419.333 kb on + strand, within BBR_RS16345 at 1419.341 kb on - strand, within BBR_RS16345 at 1419.349 kb on + strand, within BBR_RS16345 at 1419.404 kb on - strand, within BBR_RS16345 at 1419.404 kb on - strand, within BBR_RS16345 at 1419.439 kb on + strand, within BBR_RS16345 at 1419.447 kb on - strand, within BBR_RS16345 at 1419.447 kb on - strand, within BBR_RS16345 at 1419.479 kb on - strand, within BBR_RS16345 at 1419.485 kb on - strand, within BBR_RS16345 at 1419.554 kb on - strand, within BBR_RS16345 at 1419.638 kb on + strand, within BBR_RS16345 at 1419.684 kb on - strand at 1419.716 kb on - strand at 1419.739 kb on + strand at 1419.739 kb on + strand at 1419.747 kb on - strand at 1419.747 kb on - strand at 1419.762 kb on - strand at 1419.792 kb on + strand at 1419.882 kb on + strand at 1419.931 kb on - strand, within BBR_RS16350 at 1419.951 kb on + strand, within BBR_RS16350 at 1419.959 kb on - strand, within BBR_RS16350 at 1419.997 kb on + strand, within BBR_RS16350 at 1419.997 kb on + strand, within BBR_RS16350 at 1420.044 kb on - strand, within BBR_RS16350 at 1420.052 kb on + strand, within BBR_RS16350 at 1420.060 kb on - strand, within BBR_RS16350 at 1420.060 kb on - strand, within BBR_RS16350 at 1420.067 kb on + strand, within BBR_RS16350 at 1420.076 kb on + strand, within BBR_RS16350 at 1420.126 kb on - strand, within BBR_RS16350 at 1420.150 kb on - strand, within BBR_RS16350 at 1420.173 kb on + strand, within BBR_RS16350 at 1420.186 kb on - strand, within BBR_RS16350 at 1420.186 kb on - strand, within BBR_RS16350 at 1420.192 kb on + strand, within BBR_RS16350 at 1420.197 kb on + strand, within BBR_RS16350 at 1420.197 kb on + strand, within BBR_RS16350 at 1420.197 kb on + strand, within BBR_RS16350 at 1420.200 kb on - strand, within BBR_RS16350 at 1420.200 kb on - strand, within BBR_RS16350 at 1420.224 kb on + strand, within BBR_RS16350 at 1420.302 kb on - strand, within BBR_RS16350 at 1420.302 kb on - strand, within BBR_RS16350 at 1420.319 kb on - strand, within BBR_RS16350 at 1420.423 kb on - strand, within BBR_RS16350 at 1420.456 kb on + strand, within BBR_RS16350 at 1420.464 kb on - strand, within BBR_RS16350 at 1420.464 kb on - strand, within BBR_RS16350 at 1420.504 kb on - strand, within BBR_RS16350 at 1420.504 kb on - strand, within BBR_RS16350 at 1420.527 kb on + strand, within BBR_RS16350 at 1420.543 kb on + strand, within BBR_RS16350 at 1420.556 kb on + strand, within BBR_RS16350 at 1420.606 kb on - strand at 1420.657 kb on - strand at 1420.712 kb on - strand at 1420.736 kb on - strand at 1420.736 kb on - strand at 1420.758 kb on - strand, within BBR_RS16355 at 1420.785 kb on - strand, within BBR_RS16355
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,417,503 + BBR_RS16340 0.71 +0.2 1,417,545 - BBR_RS16340 0.73 -0.3 1,417,557 - BBR_RS16340 0.73 +0.1 1,417,579 + BBR_RS16340 0.74 -0.2 1,417,584 + BBR_RS16340 0.74 -0.8 1,417,629 - BBR_RS16340 0.76 +0.1 1,417,678 + BBR_RS16340 0.78 +0.8 1,417,690 + BBR_RS16340 0.79 -1.2 1,417,713 - BBR_RS16340 0.80 -0.4 1,417,715 + BBR_RS16340 0.80 +0.1 1,417,797 + BBR_RS16340 0.83 +0.7 1,417,832 + BBR_RS16340 0.84 -0.1 1,417,833 - BBR_RS16340 0.84 -0.6 1,417,833 - BBR_RS16340 0.84 -0.6 1,417,850 - BBR_RS16340 0.85 +0.2 1,417,884 + BBR_RS16340 0.87 -1.4 1,417,892 - BBR_RS16340 0.87 +0.6 1,417,925 + BBR_RS16340 0.88 +0.1 1,417,926 - BBR_RS16340 0.88 +0.3 1,417,926 - BBR_RS16340 0.88 +0.6 1,417,933 - BBR_RS16340 0.89 +0.4 1,417,933 - BBR_RS16340 0.89 +0.9 1,417,967 - BBR_RS16340 0.90 +0.1 1,418,011 - -0.1 1,418,013 + -0.6 1,418,013 + +0.6 1,418,013 + -0.2 1,418,021 - -1.2 1,418,025 + -0.0 1,418,109 + +0.1 1,418,203 + +1.3 1,418,211 - +1.2 1,418,214 + +0.4 1,418,222 - -0.5 1,418,236 - -0.1 1,418,329 + +0.1 1,418,337 - +0.1 1,418,343 + -0.5 1,418,351 - +0.4 1,418,366 + -0.7 1,418,375 + -0.1 1,418,431 + +0.3 1,418,462 + +0.2 1,418,470 - -0.4 1,418,470 - -0.4 1,418,694 + BBR_RS16345 0.15 +0.6 1,418,712 - BBR_RS16345 0.17 +0.0 1,418,712 - BBR_RS16345 0.17 +0.1 1,418,724 + BBR_RS16345 0.18 +1.1 1,418,732 - BBR_RS16345 0.18 +1.1 1,418,765 + BBR_RS16345 0.21 -0.3 1,418,813 - BBR_RS16345 0.25 -0.3 1,418,820 + BBR_RS16345 0.25 -0.8 1,418,846 + BBR_RS16345 0.27 -0.7 1,418,846 + BBR_RS16345 0.27 -0.1 1,418,846 + BBR_RS16345 0.27 -0.1 1,418,846 + BBR_RS16345 0.27 -0.0 1,418,854 - BBR_RS16345 0.28 +1.0 1,418,854 - BBR_RS16345 0.28 -0.3 1,418,854 - BBR_RS16345 0.28 +1.6 1,418,892 + BBR_RS16345 0.31 -1.0 1,418,900 - BBR_RS16345 0.31 +0.3 1,418,902 + BBR_RS16345 0.31 +0.6 1,418,902 + BBR_RS16345 0.31 +0.9 1,418,902 + BBR_RS16345 0.31 +0.9 1,418,905 - BBR_RS16345 0.32 +0.3 1,418,905 - BBR_RS16345 0.32 +0.7 1,418,910 - BBR_RS16345 0.32 +0.1 1,418,925 + BBR_RS16345 0.33 +0.1 1,418,927 + BBR_RS16345 0.33 +0.4 1,418,927 + BBR_RS16345 0.33 -0.2 1,418,927 + BBR_RS16345 0.33 +0.2 1,418,927 + BBR_RS16345 0.33 +1.0 1,418,927 + BBR_RS16345 0.33 -0.0 1,418,927 + BBR_RS16345 0.33 -0.5 1,418,927 + BBR_RS16345 0.33 +0.4 1,418,935 - BBR_RS16345 0.34 +0.2 1,418,935 - BBR_RS16345 0.34 +0.2 1,419,017 + BBR_RS16345 0.40 -0.4 1,419,055 + BBR_RS16345 0.43 +0.4 1,419,055 + BBR_RS16345 0.43 -1.2 1,419,086 + BBR_RS16345 0.46 +0.1 1,419,112 + BBR_RS16345 0.48 +0.8 1,419,112 + BBR_RS16345 0.48 +0.5 1,419,120 - BBR_RS16345 0.48 +0.3 1,419,120 - BBR_RS16345 0.48 -0.1 1,419,122 + BBR_RS16345 0.49 -0.6 1,419,130 + BBR_RS16345 0.49 -0.5 1,419,130 - BBR_RS16345 0.49 +0.7 1,419,139 + BBR_RS16345 0.50 +1.2 1,419,139 + BBR_RS16345 0.50 -0.5 1,419,144 + BBR_RS16345 0.50 -0.5 1,419,147 - BBR_RS16345 0.50 +0.6 1,419,147 - BBR_RS16345 0.50 -0.2 1,419,147 - BBR_RS16345 0.50 +0.2 1,419,152 - BBR_RS16345 0.51 +0.4 1,419,154 + BBR_RS16345 0.51 +0.6 1,419,166 + BBR_RS16345 0.52 +1.1 1,419,316 + BBR_RS16345 0.64 -0.4 1,419,324 - BBR_RS16345 0.64 +0.1 1,419,333 + BBR_RS16345 0.65 +1.2 1,419,341 - BBR_RS16345 0.66 +0.4 1,419,349 + BBR_RS16345 0.66 +0.1 1,419,404 - BBR_RS16345 0.70 -0.3 1,419,404 - BBR_RS16345 0.70 +0.8 1,419,439 + BBR_RS16345 0.73 +1.2 1,419,447 - BBR_RS16345 0.74 +0.4 1,419,447 - BBR_RS16345 0.74 +0.5 1,419,479 - BBR_RS16345 0.76 -0.0 1,419,485 - BBR_RS16345 0.77 +1.0 1,419,554 - BBR_RS16345 0.82 -0.4 1,419,638 + BBR_RS16345 0.89 +1.0 1,419,684 - +0.9 1,419,716 - -0.1 1,419,739 + -0.2 1,419,739 + +2.6 1,419,747 - -0.4 1,419,747 - +0.0 1,419,762 - +0.1 1,419,792 + -0.7 1,419,882 + -0.9 1,419,931 - BBR_RS16350 0.15 -0.8 1,419,951 + BBR_RS16350 0.18 -1.1 1,419,959 - BBR_RS16350 0.19 +0.4 1,419,997 + BBR_RS16350 0.23 -0.3 1,419,997 + BBR_RS16350 0.23 +0.7 1,420,044 - BBR_RS16350 0.29 +0.3 1,420,052 + BBR_RS16350 0.29 +2.2 1,420,060 - BBR_RS16350 0.30 +2.0 1,420,060 - BBR_RS16350 0.30 -0.4 1,420,067 + BBR_RS16350 0.31 +0.8 1,420,076 + BBR_RS16350 0.32 +0.2 1,420,126 - BBR_RS16350 0.38 +0.4 1,420,150 - BBR_RS16350 0.41 +0.4 1,420,173 + BBR_RS16350 0.44 +0.3 1,420,186 - BBR_RS16350 0.45 -0.6 1,420,186 - BBR_RS16350 0.45 +0.0 1,420,192 + BBR_RS16350 0.46 -0.3 1,420,197 + BBR_RS16350 0.46 -0.5 1,420,197 + BBR_RS16350 0.46 +0.9 1,420,197 + BBR_RS16350 0.46 +0.5 1,420,200 - BBR_RS16350 0.47 +0.7 1,420,200 - BBR_RS16350 0.47 -0.9 1,420,224 + BBR_RS16350 0.50 +0.1 1,420,302 - BBR_RS16350 0.59 +0.2 1,420,302 - BBR_RS16350 0.59 +0.1 1,420,319 - BBR_RS16350 0.61 +0.8 1,420,423 - BBR_RS16350 0.73 +0.9 1,420,456 + BBR_RS16350 0.77 -1.2 1,420,464 - BBR_RS16350 0.78 +0.1 1,420,464 - BBR_RS16350 0.78 -0.3 1,420,504 - BBR_RS16350 0.82 -0.0 1,420,504 - BBR_RS16350 0.82 -0.2 1,420,527 + BBR_RS16350 0.85 +0.3 1,420,543 + BBR_RS16350 0.87 +1.0 1,420,556 + BBR_RS16350 0.88 -0.6 1,420,606 - +1.0 1,420,657 - +0.6 1,420,712 - +0.6 1,420,736 - +1.6 1,420,736 - +0.7 1,420,758 - BBR_RS16355 0.11 +0.2 1,420,785 - BBR_RS16355 0.14 +0.6
Or see this region's nucleotide sequence