Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16290 and BBR_RS16295 are separated by 68 nucleotides BBR_RS16295 and BBR_RS16300 are separated by 83 nucleotides BBR_RS16300 and BBR_RS16305 are separated by 234 nucleotides
BBR_RS16290: BBR_RS16290 - histidine phosphatase family protein, at 1,402,278 to 1,403,123
_RS16290
BBR_RS16295: BBR_RS16295 - DNA methyltransferase, at 1,403,192 to 1,404,463
_RS16295
BBR_RS16300: BBR_RS16300 - pyridoxamine 5'-phosphate oxidase family protein, at 1,404,547 to 1,404,951
_RS16300
BBR_RS16305: BBR_RS16305 - EamA family transporter, at 1,405,186 to 1,406,121
_RS16305
Position (kb)
1403
1404
1405 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1402.197 kb on + strand at 1402.216 kb on - strand at 1402.338 kb on + strand at 1402.338 kb on + strand at 1402.338 kb on - strand at 1402.436 kb on + strand, within BBR_RS16290 at 1402.444 kb on - strand, within BBR_RS16290 at 1402.476 kb on - strand, within BBR_RS16290 at 1402.477 kb on - strand, within BBR_RS16290 at 1402.511 kb on - strand, within BBR_RS16290 at 1402.535 kb on + strand, within BBR_RS16290 at 1402.543 kb on - strand, within BBR_RS16290 at 1402.543 kb on - strand, within BBR_RS16290 at 1402.550 kb on + strand, within BBR_RS16290 at 1402.555 kb on + strand, within BBR_RS16290 at 1402.558 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.649 kb on - strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.690 kb on + strand, within BBR_RS16290 at 1402.698 kb on - strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.711 kb on + strand, within BBR_RS16290 at 1402.719 kb on - strand, within BBR_RS16290 at 1402.719 kb on - strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.728 kb on + strand, within BBR_RS16290 at 1402.736 kb on - strand, within BBR_RS16290 at 1402.785 kb on + strand, within BBR_RS16290 at 1402.791 kb on + strand, within BBR_RS16290 at 1402.817 kb on - strand, within BBR_RS16290 at 1402.853 kb on - strand, within BBR_RS16290 at 1402.853 kb on - strand, within BBR_RS16290 at 1402.900 kb on - strand, within BBR_RS16290 at 1402.921 kb on + strand, within BBR_RS16290 at 1402.929 kb on - strand, within BBR_RS16290 at 1402.990 kb on - strand, within BBR_RS16290 at 1403.019 kb on + strand, within BBR_RS16290 at 1403.032 kb on - strand, within BBR_RS16290 at 1403.163 kb on - strand at 1403.163 kb on - strand at 1403.195 kb on - strand at 1403.251 kb on + strand at 1403.261 kb on - strand at 1403.409 kb on - strand, within BBR_RS16295 at 1403.425 kb on + strand, within BBR_RS16295 at 1403.425 kb on + strand, within BBR_RS16295 at 1403.433 kb on - strand, within BBR_RS16295 at 1403.433 kb on - strand, within BBR_RS16295 at 1403.440 kb on - strand, within BBR_RS16295 at 1403.882 kb on + strand, within BBR_RS16295 at 1403.883 kb on - strand, within BBR_RS16295 at 1404.040 kb on + strand, within BBR_RS16295 at 1404.048 kb on - strand, within BBR_RS16295 at 1404.083 kb on + strand, within BBR_RS16295 at 1404.088 kb on + strand, within BBR_RS16295 at 1404.091 kb on - strand, within BBR_RS16295 at 1404.096 kb on - strand, within BBR_RS16295 at 1404.225 kb on - strand, within BBR_RS16295 at 1404.340 kb on - strand at 1404.340 kb on - strand at 1404.414 kb on - strand at 1404.428 kb on + strand at 1404.517 kb on + strand at 1404.520 kb on - strand at 1404.523 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.525 kb on - strand at 1404.548 kb on - strand at 1404.548 kb on - strand at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.667 kb on - strand, within BBR_RS16300 at 1404.702 kb on - strand, within BBR_RS16300 at 1404.762 kb on + strand, within BBR_RS16300 at 1404.765 kb on - strand, within BBR_RS16300 at 1404.781 kb on - strand, within BBR_RS16300 at 1404.782 kb on + strand, within BBR_RS16300 at 1404.790 kb on + strand, within BBR_RS16300 at 1404.798 kb on - strand, within BBR_RS16300 at 1404.860 kb on - strand, within BBR_RS16300 at 1404.887 kb on - strand, within BBR_RS16300 at 1404.952 kb on + strand at 1404.993 kb on + strand at 1405.052 kb on + strand at 1405.096 kb on - strand at 1405.187 kb on - strand at 1405.188 kb on + strand at 1405.188 kb on + strand at 1405.196 kb on - strand at 1405.255 kb on + strand at 1405.274 kb on + strand at 1405.277 kb on - strand at 1405.279 kb on + strand at 1405.281 kb on + strand, within BBR_RS16305 at 1405.287 kb on - strand, within BBR_RS16305 at 1405.287 kb on - strand, within BBR_RS16305 at 1405.291 kb on + strand, within BBR_RS16305 at 1405.341 kb on + strand, within BBR_RS16305 at 1405.348 kb on - strand, within BBR_RS16305 at 1405.393 kb on - strand, within BBR_RS16305
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,402,197 + +0.1 1,402,216 - -0.5 1,402,338 + +0.1 1,402,338 + -0.2 1,402,338 - -0.6 1,402,436 + BBR_RS16290 0.19 +0.5 1,402,444 - BBR_RS16290 0.20 -0.5 1,402,476 - BBR_RS16290 0.23 +0.1 1,402,477 - BBR_RS16290 0.24 -0.5 1,402,511 - BBR_RS16290 0.28 +1.0 1,402,535 + BBR_RS16290 0.30 +0.4 1,402,543 - BBR_RS16290 0.31 +0.1 1,402,543 - BBR_RS16290 0.31 +0.3 1,402,550 + BBR_RS16290 0.32 +0.1 1,402,555 + BBR_RS16290 0.33 +1.4 1,402,558 - BBR_RS16290 0.33 +0.5 1,402,649 - BBR_RS16290 0.44 +0.4 1,402,649 - BBR_RS16290 0.44 +0.6 1,402,649 - BBR_RS16290 0.44 -0.2 1,402,649 - BBR_RS16290 0.44 +0.8 1,402,649 - BBR_RS16290 0.44 -0.2 1,402,690 + BBR_RS16290 0.49 -1.4 1,402,690 + BBR_RS16290 0.49 +0.1 1,402,690 + BBR_RS16290 0.49 -0.1 1,402,690 + BBR_RS16290 0.49 -1.3 1,402,698 - BBR_RS16290 0.50 +0.5 1,402,711 + BBR_RS16290 0.51 +0.5 1,402,711 + BBR_RS16290 0.51 +0.8 1,402,711 + BBR_RS16290 0.51 +0.9 1,402,719 - BBR_RS16290 0.52 -0.9 1,402,719 - BBR_RS16290 0.52 -0.1 1,402,728 + BBR_RS16290 0.53 -0.8 1,402,728 + BBR_RS16290 0.53 +0.4 1,402,728 + BBR_RS16290 0.53 -0.8 1,402,728 + BBR_RS16290 0.53 +0.1 1,402,736 - BBR_RS16290 0.54 +0.2 1,402,785 + BBR_RS16290 0.60 +0.1 1,402,791 + BBR_RS16290 0.61 +0.2 1,402,817 - BBR_RS16290 0.64 -0.5 1,402,853 - BBR_RS16290 0.68 -0.5 1,402,853 - BBR_RS16290 0.68 -0.2 1,402,900 - BBR_RS16290 0.74 -0.0 1,402,921 + BBR_RS16290 0.76 -0.2 1,402,929 - BBR_RS16290 0.77 -0.4 1,402,990 - BBR_RS16290 0.84 +0.5 1,403,019 + BBR_RS16290 0.88 -0.8 1,403,032 - BBR_RS16290 0.89 -0.5 1,403,163 - -0.1 1,403,163 - -0.3 1,403,195 - -0.3 1,403,251 + -0.4 1,403,261 - +1.0 1,403,409 - BBR_RS16295 0.17 -0.7 1,403,425 + BBR_RS16295 0.18 +1.5 1,403,425 + BBR_RS16295 0.18 +0.5 1,403,433 - BBR_RS16295 0.19 +0.4 1,403,433 - BBR_RS16295 0.19 +1.2 1,403,440 - BBR_RS16295 0.19 -0.6 1,403,882 + BBR_RS16295 0.54 -0.3 1,403,883 - BBR_RS16295 0.54 +1.1 1,404,040 + BBR_RS16295 0.67 +0.2 1,404,048 - BBR_RS16295 0.67 +0.5 1,404,083 + BBR_RS16295 0.70 +0.5 1,404,088 + BBR_RS16295 0.70 -0.1 1,404,091 - BBR_RS16295 0.71 +1.5 1,404,096 - BBR_RS16295 0.71 +0.2 1,404,225 - BBR_RS16295 0.81 +0.6 1,404,340 - -0.0 1,404,340 - +0.1 1,404,414 - +0.3 1,404,428 + -0.5 1,404,517 + +0.5 1,404,520 - -0.1 1,404,523 - +1.5 1,404,525 - -0.4 1,404,525 - -1.3 1,404,525 - +0.4 1,404,525 - +0.4 1,404,548 - -0.0 1,404,548 - +0.0 1,404,667 - BBR_RS16300 0.30 +0.0 1,404,667 - BBR_RS16300 0.30 -0.4 1,404,667 - BBR_RS16300 0.30 +0.5 1,404,667 - BBR_RS16300 0.30 +0.3 1,404,667 - BBR_RS16300 0.30 -0.4 1,404,667 - BBR_RS16300 0.30 +0.6 1,404,667 - BBR_RS16300 0.30 -0.2 1,404,667 - BBR_RS16300 0.30 +0.0 1,404,702 - BBR_RS16300 0.38 +0.6 1,404,762 + BBR_RS16300 0.53 -0.5 1,404,765 - BBR_RS16300 0.54 +0.3 1,404,781 - BBR_RS16300 0.58 +0.3 1,404,782 + BBR_RS16300 0.58 +2.8 1,404,790 + BBR_RS16300 0.60 +3.2 1,404,798 - BBR_RS16300 0.62 -0.4 1,404,860 - BBR_RS16300 0.77 +0.2 1,404,887 - BBR_RS16300 0.84 +0.2 1,404,952 + -0.4 1,404,993 + +0.5 1,405,052 + +0.0 1,405,096 - +0.4 1,405,187 - -0.1 1,405,188 + -0.9 1,405,188 + +0.2 1,405,196 - +0.1 1,405,255 + +0.9 1,405,274 + -0.8 1,405,277 - -1.7 1,405,279 + -0.0 1,405,281 + BBR_RS16305 0.10 -0.1 1,405,287 - BBR_RS16305 0.11 -0.1 1,405,287 - BBR_RS16305 0.11 -0.3 1,405,291 + BBR_RS16305 0.11 -0.8 1,405,341 + BBR_RS16305 0.17 -0.2 1,405,348 - BBR_RS16305 0.17 +0.4 1,405,393 - BBR_RS16305 0.22 -0.2
Or see this region's nucleotide sequence