Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16265 and BBR_RS16270 overlap by 4 nucleotides BBR_RS16270 and BBR_RS16275 are separated by 328 nucleotides
BBR_RS16265: BBR_RS16265 - hypothetical protein, at 1,394,692 to 1,396,398
_RS16265
BBR_RS16270: BBR_RS16270 - competence protein ComEA, at 1,396,395 to 1,396,919
_RS16270
BBR_RS16275: BBR_RS16275 - leucine--tRNA ligase, at 1,397,248 to 1,400,211
_RS16275
Position (kb)
1396
1397 Strain fitness (log2 ratio)
-1
0
1
2 at 1395.395 kb on - strand, within BBR_RS16265 at 1395.395 kb on - strand, within BBR_RS16265 at 1395.442 kb on - strand, within BBR_RS16265 at 1395.442 kb on - strand, within BBR_RS16265 at 1395.461 kb on - strand, within BBR_RS16265 at 1395.552 kb on - strand, within BBR_RS16265 at 1395.643 kb on - strand, within BBR_RS16265 at 1395.656 kb on - strand, within BBR_RS16265 at 1395.656 kb on - strand, within BBR_RS16265 at 1395.668 kb on + strand, within BBR_RS16265 at 1395.680 kb on + strand, within BBR_RS16265 at 1395.693 kb on - strand, within BBR_RS16265 at 1395.833 kb on - strand, within BBR_RS16265 at 1395.871 kb on + strand, within BBR_RS16265 at 1395.986 kb on - strand, within BBR_RS16265 at 1396.011 kb on - strand, within BBR_RS16265 at 1396.056 kb on - strand, within BBR_RS16265 at 1396.083 kb on + strand, within BBR_RS16265 at 1396.083 kb on + strand, within BBR_RS16265 at 1396.086 kb on + strand, within BBR_RS16265 at 1396.086 kb on + strand, within BBR_RS16265 at 1396.087 kb on + strand, within BBR_RS16265 at 1396.207 kb on - strand, within BBR_RS16265 at 1396.211 kb on - strand, within BBR_RS16265 at 1396.213 kb on + strand, within BBR_RS16265 at 1396.216 kb on - strand, within BBR_RS16265 at 1396.236 kb on + strand at 1396.271 kb on + strand at 1396.421 kb on - strand at 1396.570 kb on + strand, within BBR_RS16270 at 1396.606 kb on + strand, within BBR_RS16270 at 1396.611 kb on + strand, within BBR_RS16270 at 1396.619 kb on - strand, within BBR_RS16270 at 1396.619 kb on - strand, within BBR_RS16270 at 1396.619 kb on - strand, within BBR_RS16270 at 1396.619 kb on - strand, within BBR_RS16270 at 1396.619 kb on - strand, within BBR_RS16270 at 1396.645 kb on + strand, within BBR_RS16270 at 1396.711 kb on - strand, within BBR_RS16270 at 1396.789 kb on + strand, within BBR_RS16270 at 1396.797 kb on - strand, within BBR_RS16270 at 1396.802 kb on - strand, within BBR_RS16270 at 1396.815 kb on - strand, within BBR_RS16270 at 1396.830 kb on + strand, within BBR_RS16270 at 1396.852 kb on + strand, within BBR_RS16270 at 1396.877 kb on + strand at 1396.877 kb on + strand at 1396.887 kb on + strand at 1396.898 kb on + strand at 1396.912 kb on - strand at 1396.965 kb on + strand at 1396.973 kb on - strand at 1397.060 kb on + strand at 1397.083 kb on + strand at 1397.091 kb on - strand at 1397.096 kb on - strand at 1397.101 kb on + strand at 1397.137 kb on + strand at 1397.144 kb on - strand at 1397.165 kb on - strand at 1397.208 kb on + strand at 1397.223 kb on + strand at 1397.223 kb on + strand at 1397.253 kb on - strand at 1397.267 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,395,395 - BBR_RS16265 0.41 +0.1 1,395,395 - BBR_RS16265 0.41 -0.0 1,395,442 - BBR_RS16265 0.44 +0.1 1,395,442 - BBR_RS16265 0.44 -0.6 1,395,461 - BBR_RS16265 0.45 -1.6 1,395,552 - BBR_RS16265 0.50 -0.3 1,395,643 - BBR_RS16265 0.56 +1.1 1,395,656 - BBR_RS16265 0.56 +0.8 1,395,656 - BBR_RS16265 0.56 +0.6 1,395,668 + BBR_RS16265 0.57 +1.3 1,395,680 + BBR_RS16265 0.58 -0.5 1,395,693 - BBR_RS16265 0.59 -0.1 1,395,833 - BBR_RS16265 0.67 +0.6 1,395,871 + BBR_RS16265 0.69 -0.8 1,395,986 - BBR_RS16265 0.76 -0.9 1,396,011 - BBR_RS16265 0.77 +1.0 1,396,056 - BBR_RS16265 0.80 +0.4 1,396,083 + BBR_RS16265 0.81 +1.1 1,396,083 + BBR_RS16265 0.81 +0.1 1,396,086 + BBR_RS16265 0.82 +0.4 1,396,086 + BBR_RS16265 0.82 +0.0 1,396,087 + BBR_RS16265 0.82 -0.2 1,396,207 - BBR_RS16265 0.89 +0.6 1,396,211 - BBR_RS16265 0.89 -0.1 1,396,213 + BBR_RS16265 0.89 -1.5 1,396,216 - BBR_RS16265 0.89 -0.1 1,396,236 + +0.2 1,396,271 + -0.4 1,396,421 - -0.4 1,396,570 + BBR_RS16270 0.33 +0.2 1,396,606 + BBR_RS16270 0.40 -0.2 1,396,611 + BBR_RS16270 0.41 -0.0 1,396,619 - BBR_RS16270 0.43 -0.1 1,396,619 - BBR_RS16270 0.43 -0.3 1,396,619 - BBR_RS16270 0.43 -0.2 1,396,619 - BBR_RS16270 0.43 +0.6 1,396,619 - BBR_RS16270 0.43 -0.1 1,396,645 + BBR_RS16270 0.48 +1.5 1,396,711 - BBR_RS16270 0.60 +1.4 1,396,789 + BBR_RS16270 0.75 -0.4 1,396,797 - BBR_RS16270 0.77 -0.0 1,396,802 - BBR_RS16270 0.78 +0.1 1,396,815 - BBR_RS16270 0.80 +0.4 1,396,830 + BBR_RS16270 0.83 +0.6 1,396,852 + BBR_RS16270 0.87 +0.6 1,396,877 + +0.4 1,396,877 + -0.1 1,396,887 + -0.1 1,396,898 + -0.2 1,396,912 - -0.4 1,396,965 + +1.0 1,396,973 - +0.4 1,397,060 + +2.2 1,397,083 + -1.1 1,397,091 - -1.1 1,397,096 - -0.0 1,397,101 + -0.2 1,397,137 + -0.1 1,397,144 - -0.5 1,397,165 - +0.3 1,397,208 + +0.4 1,397,223 + +0.1 1,397,223 + -0.3 1,397,253 - -1.4 1,397,267 - +0.2
Or see this region's nucleotide sequence