Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16075 and BBR_RS16080 overlap by 17 nucleotides BBR_RS16080 and BBR_RS16085 are separated by 362 nucleotides BBR_RS16085 and BBR_RS16090 are separated by 205 nucleotides BBR_RS16090 and BBR_RS16095 are separated by 82 nucleotides
BBR_RS16075: BBR_RS16075 - hypothetical protein, at 1,357,018 to 1,357,914
_RS16075
BBR_RS16080: BBR_RS16080 - DUF1287 domain-containing protein, at 1,357,898 to 1,358,260
_RS16080
BBR_RS16085: BBR_RS16085 - anthranilate synthase component I, at 1,358,623 to 1,360,179
_RS16085
BBR_RS16090: BBR_RS16090 - phosphoribosyl-AMP cyclohydrolase, at 1,360,385 to 1,360,780
_RS16090
BBR_RS16095: BBR_RS16095 - imidazole glycerol phosphate synthase subunit HisF, at 1,360,863 to 1,361,633
_RS16095
Position (kb)
1358
1359
1360
1361 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1357.707 kb on - strand, within BBR_RS16075 at 1357.860 kb on - strand at 1357.908 kb on + strand at 1358.076 kb on + strand, within BBR_RS16080 at 1358.134 kb on - strand, within BBR_RS16080 at 1358.163 kb on + strand, within BBR_RS16080 at 1358.186 kb on - strand, within BBR_RS16080 at 1358.294 kb on + strand at 1358.315 kb on - strand at 1358.352 kb on + strand at 1358.369 kb on + strand at 1358.417 kb on + strand at 1358.552 kb on - strand at 1358.597 kb on + strand at 1358.601 kb on + strand at 1358.628 kb on - strand at 1358.628 kb on - strand at 1358.628 kb on - strand at 1358.717 kb on - strand at 1358.717 kb on - strand at 1358.732 kb on + strand at 1358.764 kb on + strand at 1358.767 kb on - strand at 1358.767 kb on - strand at 1358.913 kb on - strand, within BBR_RS16085 at 1358.935 kb on - strand, within BBR_RS16085 at 1358.945 kb on - strand, within BBR_RS16085 at 1358.965 kb on - strand, within BBR_RS16085 at 1359.017 kb on + strand, within BBR_RS16085 at 1359.047 kb on + strand, within BBR_RS16085 at 1359.161 kb on - strand, within BBR_RS16085 at 1359.161 kb on - strand, within BBR_RS16085 at 1359.260 kb on + strand, within BBR_RS16085 at 1359.285 kb on - strand, within BBR_RS16085 at 1359.293 kb on - strand, within BBR_RS16085 at 1359.303 kb on + strand, within BBR_RS16085 at 1359.324 kb on + strand, within BBR_RS16085 at 1359.332 kb on - strand, within BBR_RS16085 at 1359.332 kb on - strand, within BBR_RS16085 at 1359.388 kb on + strand, within BBR_RS16085 at 1359.425 kb on - strand, within BBR_RS16085 at 1359.580 kb on + strand, within BBR_RS16085 at 1359.639 kb on - strand, within BBR_RS16085 at 1359.655 kb on + strand, within BBR_RS16085 at 1359.677 kb on + strand, within BBR_RS16085 at 1359.778 kb on - strand, within BBR_RS16085 at 1359.790 kb on - strand, within BBR_RS16085 at 1359.837 kb on - strand, within BBR_RS16085 at 1359.843 kb on + strand, within BBR_RS16085 at 1359.871 kb on - strand, within BBR_RS16085 at 1359.888 kb on - strand, within BBR_RS16085 at 1359.939 kb on + strand, within BBR_RS16085 at 1360.081 kb on + strand at 1360.097 kb on - strand at 1360.122 kb on + strand at 1360.140 kb on - strand at 1360.242 kb on + strand at 1360.358 kb on - strand at 1360.390 kb on - strand at 1360.415 kb on - strand at 1360.478 kb on - strand, within BBR_RS16090 at 1360.478 kb on - strand, within BBR_RS16090 at 1360.493 kb on - strand, within BBR_RS16090 at 1360.522 kb on - strand, within BBR_RS16090 at 1360.523 kb on - strand, within BBR_RS16090 at 1360.525 kb on + strand, within BBR_RS16090 at 1360.536 kb on - strand, within BBR_RS16090 at 1360.546 kb on + strand, within BBR_RS16090 at 1360.546 kb on + strand, within BBR_RS16090 at 1360.600 kb on - strand, within BBR_RS16090 at 1360.625 kb on + strand, within BBR_RS16090 at 1360.646 kb on - strand, within BBR_RS16090 at 1360.682 kb on + strand, within BBR_RS16090 at 1360.697 kb on + strand, within BBR_RS16090 at 1360.697 kb on + strand, within BBR_RS16090 at 1360.697 kb on + strand, within BBR_RS16090 at 1360.716 kb on + strand, within BBR_RS16090 at 1360.749 kb on - strand at 1360.750 kb on - strand at 1360.768 kb on - strand at 1360.768 kb on - strand at 1360.806 kb on - strand at 1360.873 kb on + strand at 1360.881 kb on - strand at 1360.922 kb on - strand at 1360.922 kb on - strand at 1361.026 kb on + strand, within BBR_RS16095 at 1361.043 kb on - strand, within BBR_RS16095 at 1361.127 kb on + strand, within BBR_RS16095
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,357,707 - BBR_RS16075 0.77 -1.1 1,357,860 - -0.0 1,357,908 + +0.7 1,358,076 + BBR_RS16080 0.49 +0.3 1,358,134 - BBR_RS16080 0.65 -1.8 1,358,163 + BBR_RS16080 0.73 -0.1 1,358,186 - BBR_RS16080 0.79 -0.2 1,358,294 + +0.1 1,358,315 - +0.2 1,358,352 + +1.3 1,358,369 + +0.3 1,358,417 + -0.5 1,358,552 - +0.6 1,358,597 + -0.0 1,358,601 + +0.0 1,358,628 - +0.2 1,358,628 - -0.7 1,358,628 - +0.5 1,358,717 - -0.8 1,358,717 - +0.0 1,358,732 + +0.0 1,358,764 + -0.4 1,358,767 - -0.2 1,358,767 - -0.4 1,358,913 - BBR_RS16085 0.19 +0.0 1,358,935 - BBR_RS16085 0.20 +0.2 1,358,945 - BBR_RS16085 0.21 +0.3 1,358,965 - BBR_RS16085 0.22 -0.5 1,359,017 + BBR_RS16085 0.25 +0.6 1,359,047 + BBR_RS16085 0.27 +1.3 1,359,161 - BBR_RS16085 0.35 -0.2 1,359,161 - BBR_RS16085 0.35 +0.0 1,359,260 + BBR_RS16085 0.41 -0.8 1,359,285 - BBR_RS16085 0.43 -0.4 1,359,293 - BBR_RS16085 0.43 -0.2 1,359,303 + BBR_RS16085 0.44 +0.1 1,359,324 + BBR_RS16085 0.45 +0.3 1,359,332 - BBR_RS16085 0.46 +0.9 1,359,332 - BBR_RS16085 0.46 -0.4 1,359,388 + BBR_RS16085 0.49 +0.4 1,359,425 - BBR_RS16085 0.52 -0.0 1,359,580 + BBR_RS16085 0.61 +1.0 1,359,639 - BBR_RS16085 0.65 -0.2 1,359,655 + BBR_RS16085 0.66 +0.5 1,359,677 + BBR_RS16085 0.68 +0.1 1,359,778 - BBR_RS16085 0.74 +0.1 1,359,790 - BBR_RS16085 0.75 +0.9 1,359,837 - BBR_RS16085 0.78 +0.4 1,359,843 + BBR_RS16085 0.78 +1.5 1,359,871 - BBR_RS16085 0.80 +0.4 1,359,888 - BBR_RS16085 0.81 -1.2 1,359,939 + BBR_RS16085 0.85 +0.6 1,360,081 + -1.1 1,360,097 - -0.2 1,360,122 + -0.3 1,360,140 - +2.5 1,360,242 + +0.2 1,360,358 - -0.4 1,360,390 - +0.3 1,360,415 - +0.6 1,360,478 - BBR_RS16090 0.23 +0.9 1,360,478 - BBR_RS16090 0.23 -1.4 1,360,493 - BBR_RS16090 0.27 +0.0 1,360,522 - BBR_RS16090 0.35 +0.0 1,360,523 - BBR_RS16090 0.35 +0.1 1,360,525 + BBR_RS16090 0.35 -0.0 1,360,536 - BBR_RS16090 0.38 -0.9 1,360,546 + BBR_RS16090 0.41 +0.2 1,360,546 + BBR_RS16090 0.41 -0.4 1,360,600 - BBR_RS16090 0.54 -1.7 1,360,625 + BBR_RS16090 0.61 +0.7 1,360,646 - BBR_RS16090 0.66 +1.0 1,360,682 + BBR_RS16090 0.75 -0.5 1,360,697 + BBR_RS16090 0.79 +0.1 1,360,697 + BBR_RS16090 0.79 -0.1 1,360,697 + BBR_RS16090 0.79 -0.3 1,360,716 + BBR_RS16090 0.84 -0.9 1,360,749 - -0.1 1,360,750 - +0.3 1,360,768 - -0.5 1,360,768 - -0.1 1,360,806 - -0.5 1,360,873 + -0.1 1,360,881 - +0.1 1,360,922 - -1.6 1,360,922 - -1.1 1,361,026 + BBR_RS16095 0.21 -1.0 1,361,043 - BBR_RS16095 0.23 +0.1 1,361,127 + BBR_RS16095 0.34 +0.2
Or see this region's nucleotide sequence