Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16005 and BBR_RS16010 are separated by 194 nucleotides BBR_RS16010 and BBR_RS16015 are separated by 331 nucleotides BBR_RS16015 and BBR_RS16020 are separated by 2 nucleotides
BBR_RS16005: BBR_RS16005 - ABC transporter, at 1,347,124 to 1,348,026
_RS16005
BBR_RS16010: BBR_RS16010 - hypothetical protein, at 1,348,221 to 1,348,754
_RS16010
BBR_RS16015: BBR_RS16015 - DUF2089 domain-containing protein, at 1,349,086 to 1,349,388
_RS16015
BBR_RS16020: BBR_RS16020 - hypothetical protein, at 1,349,391 to 1,349,735
_RS16020
Position (kb)
1348
1349 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1347.260 kb on + strand, within BBR_RS16005 at 1347.260 kb on + strand, within BBR_RS16005 at 1347.270 kb on + strand, within BBR_RS16005 at 1347.283 kb on - strand, within BBR_RS16005 at 1347.286 kb on - strand, within BBR_RS16005 at 1347.397 kb on + strand, within BBR_RS16005 at 1347.494 kb on + strand, within BBR_RS16005 at 1347.512 kb on + strand, within BBR_RS16005 at 1347.512 kb on + strand, within BBR_RS16005 at 1347.514 kb on + strand, within BBR_RS16005 at 1347.515 kb on - strand, within BBR_RS16005 at 1347.520 kb on - strand, within BBR_RS16005 at 1347.520 kb on - strand, within BBR_RS16005 at 1347.522 kb on + strand, within BBR_RS16005 at 1347.529 kb on + strand, within BBR_RS16005 at 1347.532 kb on - strand, within BBR_RS16005 at 1347.536 kb on + strand, within BBR_RS16005 at 1347.537 kb on - strand, within BBR_RS16005 at 1347.542 kb on - strand, within BBR_RS16005 at 1347.559 kb on + strand, within BBR_RS16005 at 1347.562 kb on - strand, within BBR_RS16005 at 1347.587 kb on - strand, within BBR_RS16005 at 1347.591 kb on + strand, within BBR_RS16005 at 1347.601 kb on + strand, within BBR_RS16005 at 1347.638 kb on + strand, within BBR_RS16005 at 1347.675 kb on - strand, within BBR_RS16005 at 1347.684 kb on - strand, within BBR_RS16005 at 1347.701 kb on + strand, within BBR_RS16005 at 1347.768 kb on - strand, within BBR_RS16005 at 1347.833 kb on + strand, within BBR_RS16005 at 1347.833 kb on + strand, within BBR_RS16005 at 1347.833 kb on + strand, within BBR_RS16005 at 1347.847 kb on + strand, within BBR_RS16005 at 1347.855 kb on - strand, within BBR_RS16005 at 1347.855 kb on - strand, within BBR_RS16005 at 1348.043 kb on - strand at 1348.043 kb on - strand at 1348.043 kb on - strand at 1348.089 kb on - strand at 1348.116 kb on + strand at 1348.117 kb on + strand at 1348.138 kb on - strand at 1348.335 kb on - strand, within BBR_RS16010 at 1348.389 kb on - strand, within BBR_RS16010 at 1348.427 kb on - strand, within BBR_RS16010 at 1348.429 kb on - strand, within BBR_RS16010 at 1348.485 kb on + strand, within BBR_RS16010 at 1348.485 kb on + strand, within BBR_RS16010 at 1348.491 kb on - strand, within BBR_RS16010 at 1348.516 kb on - strand, within BBR_RS16010 at 1348.537 kb on + strand, within BBR_RS16010 at 1348.692 kb on + strand, within BBR_RS16010 at 1348.718 kb on + strand at 1348.756 kb on - strand at 1348.783 kb on - strand at 1348.877 kb on + strand at 1348.885 kb on - strand at 1348.937 kb on + strand at 1348.991 kb on - strand at 1349.174 kb on + strand, within BBR_RS16015 at 1349.174 kb on + strand, within BBR_RS16015 at 1349.174 kb on + strand, within BBR_RS16015 at 1349.182 kb on - strand, within BBR_RS16015 at 1349.245 kb on - strand, within BBR_RS16015 at 1349.286 kb on - strand, within BBR_RS16015 at 1349.295 kb on + strand, within BBR_RS16015 at 1349.312 kb on - strand, within BBR_RS16015 at 1349.344 kb on + strand, within BBR_RS16015 at 1349.375 kb on + strand at 1349.381 kb on - strand at 1349.387 kb on + strand at 1349.387 kb on + strand at 1349.391 kb on - strand at 1349.476 kb on - strand, within BBR_RS16020 at 1349.491 kb on - strand, within BBR_RS16020 at 1349.564 kb on - strand, within BBR_RS16020 at 1349.599 kb on + strand, within BBR_RS16020
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,347,260 + BBR_RS16005 0.15 -1.3 1,347,260 + BBR_RS16005 0.15 +0.2 1,347,270 + BBR_RS16005 0.16 -1.1 1,347,283 - BBR_RS16005 0.18 -0.4 1,347,286 - BBR_RS16005 0.18 +0.4 1,347,397 + BBR_RS16005 0.30 +0.4 1,347,494 + BBR_RS16005 0.41 -2.5 1,347,512 + BBR_RS16005 0.43 -0.3 1,347,512 + BBR_RS16005 0.43 -0.9 1,347,514 + BBR_RS16005 0.43 -0.2 1,347,515 - BBR_RS16005 0.43 -0.7 1,347,520 - BBR_RS16005 0.44 +0.0 1,347,520 - BBR_RS16005 0.44 +0.2 1,347,522 + BBR_RS16005 0.44 -0.6 1,347,529 + BBR_RS16005 0.45 +0.4 1,347,532 - BBR_RS16005 0.45 +0.5 1,347,536 + BBR_RS16005 0.46 -0.2 1,347,537 - BBR_RS16005 0.46 -0.0 1,347,542 - BBR_RS16005 0.46 -0.0 1,347,559 + BBR_RS16005 0.48 +0.3 1,347,562 - BBR_RS16005 0.49 +0.9 1,347,587 - BBR_RS16005 0.51 -0.1 1,347,591 + BBR_RS16005 0.52 +0.2 1,347,601 + BBR_RS16005 0.53 -0.8 1,347,638 + BBR_RS16005 0.57 -1.1 1,347,675 - BBR_RS16005 0.61 +0.8 1,347,684 - BBR_RS16005 0.62 +0.1 1,347,701 + BBR_RS16005 0.64 -0.8 1,347,768 - BBR_RS16005 0.71 -1.3 1,347,833 + BBR_RS16005 0.79 +0.6 1,347,833 + BBR_RS16005 0.79 -1.2 1,347,833 + BBR_RS16005 0.79 -0.4 1,347,847 + BBR_RS16005 0.80 -0.9 1,347,855 - BBR_RS16005 0.81 -0.4 1,347,855 - BBR_RS16005 0.81 +0.2 1,348,043 - +0.8 1,348,043 - +0.1 1,348,043 - +0.6 1,348,089 - -0.7 1,348,116 + -0.3 1,348,117 + -0.3 1,348,138 - +0.3 1,348,335 - BBR_RS16010 0.21 +1.0 1,348,389 - BBR_RS16010 0.31 -0.5 1,348,427 - BBR_RS16010 0.39 -0.6 1,348,429 - BBR_RS16010 0.39 +0.7 1,348,485 + BBR_RS16010 0.49 -0.3 1,348,485 + BBR_RS16010 0.49 +0.3 1,348,491 - BBR_RS16010 0.51 -0.3 1,348,516 - BBR_RS16010 0.55 +0.6 1,348,537 + BBR_RS16010 0.59 +1.2 1,348,692 + BBR_RS16010 0.88 +0.3 1,348,718 + +1.0 1,348,756 - -0.1 1,348,783 - +0.5 1,348,877 + +0.2 1,348,885 - +0.9 1,348,937 + +0.0 1,348,991 - -1.1 1,349,174 + BBR_RS16015 0.29 -0.3 1,349,174 + BBR_RS16015 0.29 +0.2 1,349,174 + BBR_RS16015 0.29 +0.7 1,349,182 - BBR_RS16015 0.32 +1.2 1,349,245 - BBR_RS16015 0.52 +2.2 1,349,286 - BBR_RS16015 0.66 +0.2 1,349,295 + BBR_RS16015 0.69 -0.1 1,349,312 - BBR_RS16015 0.75 -0.4 1,349,344 + BBR_RS16015 0.85 +0.5 1,349,375 + +0.1 1,349,381 - -0.3 1,349,387 + -0.7 1,349,387 + -0.5 1,349,391 - -0.2 1,349,476 - BBR_RS16020 0.25 +0.8 1,349,491 - BBR_RS16020 0.29 -0.4 1,349,564 - BBR_RS16020 0.50 +0.5 1,349,599 + BBR_RS16020 0.60 +0.0
Or see this region's nucleotide sequence