Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15490 and BBR_RS15495 are separated by 65 nucleotides BBR_RS15495 and BBR_RS15500 overlap by 1 nucleotides BBR_RS15500 and BBR_RS15505 are separated by 244 nucleotides
BBR_RS15490: BBR_RS15490 - short-chain dehydrogenase, at 1,246,599 to 1,247,306
_RS15490
BBR_RS15495: BBR_RS15495 - ABC transporter ATP-binding protein, at 1,247,372 to 1,248,073
_RS15495
BBR_RS15500: BBR_RS15500 - cobalt ABC transporter permease, at 1,248,073 to 1,248,704
_RS15500
BBR_RS15505: BBR_RS15505 - pyridoxal phosphate-dependent aminotransferase, at 1,248,949 to 1,250,139
_RS15505
Position (kb)
1248
1249 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1247.081 kb on + strand, within BBR_RS15490 at 1247.081 kb on + strand, within BBR_RS15490 at 1247.083 kb on + strand, within BBR_RS15490 at 1247.084 kb on - strand, within BBR_RS15490 at 1247.089 kb on - strand, within BBR_RS15490 at 1247.089 kb on - strand, within BBR_RS15490 at 1247.145 kb on - strand, within BBR_RS15490 at 1247.157 kb on + strand, within BBR_RS15490 at 1247.233 kb on + strand, within BBR_RS15490 at 1247.274 kb on - strand at 1247.330 kb on - strand at 1247.342 kb on + strand at 1247.362 kb on + strand at 1247.387 kb on - strand at 1247.391 kb on + strand at 1247.502 kb on + strand, within BBR_RS15495 at 1247.510 kb on - strand, within BBR_RS15495 at 1247.512 kb on + strand, within BBR_RS15495 at 1247.558 kb on + strand, within BBR_RS15495 at 1247.603 kb on - strand, within BBR_RS15495 at 1247.772 kb on + strand, within BBR_RS15495 at 1247.876 kb on - strand, within BBR_RS15495 at 1247.900 kb on + strand, within BBR_RS15495 at 1247.924 kb on + strand, within BBR_RS15495 at 1247.929 kb on + strand, within BBR_RS15495 at 1247.929 kb on + strand, within BBR_RS15495 at 1247.937 kb on - strand, within BBR_RS15495 at 1247.937 kb on - strand, within BBR_RS15495 at 1247.988 kb on - strand, within BBR_RS15495 at 1247.992 kb on + strand, within BBR_RS15495 at 1248.021 kb on - strand at 1248.079 kb on - strand at 1248.190 kb on - strand, within BBR_RS15500 at 1248.191 kb on + strand, within BBR_RS15500 at 1248.191 kb on + strand, within BBR_RS15500 at 1248.212 kb on + strand, within BBR_RS15500 at 1248.241 kb on + strand, within BBR_RS15500 at 1248.295 kb on + strand, within BBR_RS15500 at 1248.303 kb on - strand, within BBR_RS15500 at 1248.325 kb on + strand, within BBR_RS15500 at 1248.392 kb on - strand, within BBR_RS15500 at 1248.397 kb on + strand, within BBR_RS15500 at 1248.402 kb on - strand, within BBR_RS15500 at 1248.512 kb on - strand, within BBR_RS15500 at 1248.530 kb on - strand, within BBR_RS15500 at 1248.537 kb on + strand, within BBR_RS15500 at 1248.545 kb on - strand, within BBR_RS15500 at 1248.569 kb on + strand, within BBR_RS15500 at 1248.571 kb on + strand, within BBR_RS15500 at 1248.584 kb on - strand, within BBR_RS15500 at 1248.584 kb on - strand, within BBR_RS15500 at 1248.589 kb on - strand, within BBR_RS15500 at 1248.703 kb on + strand at 1248.703 kb on + strand at 1248.801 kb on + strand at 1248.818 kb on + strand at 1248.843 kb on + strand at 1248.963 kb on + strand at 1248.963 kb on - strand at 1248.963 kb on - strand at 1248.998 kb on + strand at 1248.998 kb on + strand at 1248.998 kb on + strand at 1249.006 kb on - strand at 1249.006 kb on - strand at 1249.040 kb on - strand at 1249.147 kb on - strand, within BBR_RS15505 at 1249.168 kb on - strand, within BBR_RS15505 at 1249.170 kb on + strand, within BBR_RS15505 at 1249.204 kb on - strand, within BBR_RS15505 at 1249.204 kb on - strand, within BBR_RS15505 at 1249.221 kb on + strand, within BBR_RS15505 at 1249.245 kb on + strand, within BBR_RS15505 at 1249.246 kb on + strand, within BBR_RS15505 at 1249.294 kb on - strand, within BBR_RS15505 at 1249.296 kb on + strand, within BBR_RS15505 at 1249.299 kb on - strand, within BBR_RS15505 at 1249.299 kb on - strand, within BBR_RS15505 at 1249.333 kb on + strand, within BBR_RS15505 at 1249.353 kb on + strand, within BBR_RS15505 at 1249.414 kb on + strand, within BBR_RS15505 at 1249.414 kb on + strand, within BBR_RS15505 at 1249.423 kb on - strand, within BBR_RS15505 at 1249.456 kb on + strand, within BBR_RS15505 at 1249.464 kb on - strand, within BBR_RS15505 at 1249.464 kb on - strand, within BBR_RS15505 at 1249.464 kb on - strand, within BBR_RS15505 at 1249.521 kb on - strand, within BBR_RS15505 at 1249.534 kb on - strand, within BBR_RS15505 at 1249.577 kb on + strand, within BBR_RS15505 at 1249.610 kb on + strand, within BBR_RS15505 at 1249.657 kb on - strand, within BBR_RS15505
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,247,081 + BBR_RS15490 0.68 +0.5 1,247,081 + BBR_RS15490 0.68 -0.4 1,247,083 + BBR_RS15490 0.68 -0.6 1,247,084 - BBR_RS15490 0.69 +0.1 1,247,089 - BBR_RS15490 0.69 -0.0 1,247,089 - BBR_RS15490 0.69 -0.7 1,247,145 - BBR_RS15490 0.77 -0.1 1,247,157 + BBR_RS15490 0.79 +0.3 1,247,233 + BBR_RS15490 0.90 +0.4 1,247,274 - +1.5 1,247,330 - -0.6 1,247,342 + +0.7 1,247,362 + +0.0 1,247,387 - -0.1 1,247,391 + -0.3 1,247,502 + BBR_RS15495 0.19 +0.2 1,247,510 - BBR_RS15495 0.20 +0.2 1,247,512 + BBR_RS15495 0.20 -1.0 1,247,558 + BBR_RS15495 0.26 -0.1 1,247,603 - BBR_RS15495 0.33 +0.0 1,247,772 + BBR_RS15495 0.57 +0.4 1,247,876 - BBR_RS15495 0.72 -0.7 1,247,900 + BBR_RS15495 0.75 -0.4 1,247,924 + BBR_RS15495 0.79 +0.2 1,247,929 + BBR_RS15495 0.79 -0.3 1,247,929 + BBR_RS15495 0.79 +0.3 1,247,937 - BBR_RS15495 0.80 +0.2 1,247,937 - BBR_RS15495 0.80 -0.5 1,247,988 - BBR_RS15495 0.88 +0.2 1,247,992 + BBR_RS15495 0.88 -0.5 1,248,021 - +0.7 1,248,079 - -0.4 1,248,190 - BBR_RS15500 0.19 +0.4 1,248,191 + BBR_RS15500 0.19 +0.6 1,248,191 + BBR_RS15500 0.19 +0.8 1,248,212 + BBR_RS15500 0.22 +0.4 1,248,241 + BBR_RS15500 0.27 +0.0 1,248,295 + BBR_RS15500 0.35 +0.3 1,248,303 - BBR_RS15500 0.36 +0.4 1,248,325 + BBR_RS15500 0.40 +0.0 1,248,392 - BBR_RS15500 0.50 +0.1 1,248,397 + BBR_RS15500 0.51 +0.4 1,248,402 - BBR_RS15500 0.52 -0.8 1,248,512 - BBR_RS15500 0.69 +0.5 1,248,530 - BBR_RS15500 0.72 +0.4 1,248,537 + BBR_RS15500 0.73 -1.5 1,248,545 - BBR_RS15500 0.75 +0.2 1,248,569 + BBR_RS15500 0.78 -1.8 1,248,571 + BBR_RS15500 0.79 +0.9 1,248,584 - BBR_RS15500 0.81 +0.1 1,248,584 - BBR_RS15500 0.81 +0.1 1,248,589 - BBR_RS15500 0.82 -0.1 1,248,703 + -0.2 1,248,703 + -0.6 1,248,801 + +0.5 1,248,818 + -0.1 1,248,843 + +0.1 1,248,963 + +0.3 1,248,963 - +0.7 1,248,963 - -0.5 1,248,998 + +0.1 1,248,998 + -1.1 1,248,998 + +0.8 1,249,006 - +0.3 1,249,006 - -0.2 1,249,040 - -0.8 1,249,147 - BBR_RS15505 0.17 +2.1 1,249,168 - BBR_RS15505 0.18 -0.5 1,249,170 + BBR_RS15505 0.19 +0.2 1,249,204 - BBR_RS15505 0.21 -0.2 1,249,204 - BBR_RS15505 0.21 +0.3 1,249,221 + BBR_RS15505 0.23 +0.4 1,249,245 + BBR_RS15505 0.25 +0.2 1,249,246 + BBR_RS15505 0.25 -0.4 1,249,294 - BBR_RS15505 0.29 -0.1 1,249,296 + BBR_RS15505 0.29 +0.3 1,249,299 - BBR_RS15505 0.29 +0.2 1,249,299 - BBR_RS15505 0.29 +0.1 1,249,333 + BBR_RS15505 0.32 -0.8 1,249,353 + BBR_RS15505 0.34 +0.7 1,249,414 + BBR_RS15505 0.39 +0.6 1,249,414 + BBR_RS15505 0.39 -0.0 1,249,423 - BBR_RS15505 0.40 +0.8 1,249,456 + BBR_RS15505 0.43 +1.3 1,249,464 - BBR_RS15505 0.43 -0.4 1,249,464 - BBR_RS15505 0.43 +0.3 1,249,464 - BBR_RS15505 0.43 -0.4 1,249,521 - BBR_RS15505 0.48 -0.7 1,249,534 - BBR_RS15505 0.49 -0.4 1,249,577 + BBR_RS15505 0.53 +0.3 1,249,610 + BBR_RS15505 0.55 +0.6 1,249,657 - BBR_RS15505 0.59 +0.2
Or see this region's nucleotide sequence