Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15315 and BBR_RS15320 are separated by 105 nucleotides BBR_RS15320 and BBR_RS15325 are separated by 216 nucleotides BBR_RS15325 and BBR_RS15330 are separated by 10 nucleotides BBR_RS15330 and BBR_RS15335 are separated by 24 nucleotides
BBR_RS15315: BBR_RS15315 - ATPase AAA, at 1,210,462 to 1,211,247
_RS15315
BBR_RS15320: BBR_RS15320 - hypothetical protein, at 1,211,353 to 1,211,622
_RS15320
BBR_RS15325: BBR_RS15325 - hypothetical protein, at 1,211,839 to 1,212,024
_RS15325
BBR_RS15330: BBR_RS15330 - hypothetical protein, at 1,212,035 to 1,212,556
_RS15330
BBR_RS15335: BBR_RS15335 - hypothetical protein, at 1,212,581 to 1,213,315
_RS15335
Position (kb) 1211
1212
1213 Strain fitness (log2 ratio) -1
0
1
2 at 1211.380 kb on - strand, within BBR_RS15320 at 1211.504 kb on - strand, within BBR_RS15320 at 1211.504 kb on - strand, within BBR_RS15320 at 1211.510 kb on - strand, within BBR_RS15320 at 1211.544 kb on + strand, within BBR_RS15320 at 1211.552 kb on - strand, within BBR_RS15320 at 1211.613 kb on + strand at 1211.632 kb on + strand at 1211.667 kb on + strand at 1211.777 kb on - strand at 1211.845 kb on - strand at 1211.845 kb on - strand at 1211.890 kb on + strand, within BBR_RS15325 at 1211.912 kb on - strand, within BBR_RS15325 at 1211.943 kb on + strand, within BBR_RS15325 at 1211.943 kb on + strand, within BBR_RS15325 at 1211.965 kb on - strand, within BBR_RS15325 at 1211.983 kb on - strand, within BBR_RS15325 at 1211.983 kb on - strand, within BBR_RS15325 at 1211.996 kb on - strand, within BBR_RS15325 at 1212.041 kb on + strand at 1212.079 kb on + strand at 1212.079 kb on + strand at 1212.079 kb on + strand at 1212.180 kb on - strand, within BBR_RS15330 at 1212.180 kb on - strand, within BBR_RS15330 at 1212.226 kb on - strand, within BBR_RS15330 at 1212.311 kb on + strand, within BBR_RS15330 at 1212.338 kb on - strand, within BBR_RS15330 at 1212.346 kb on - strand, within BBR_RS15330 at 1212.553 kb on + strand at 1212.554 kb on + strand at 1212.562 kb on - strand at 1212.697 kb on + strand, within BBR_RS15335 at 1212.706 kb on + strand, within BBR_RS15335 at 1212.793 kb on - strand, within BBR_RS15335 at 1212.836 kb on + strand, within BBR_RS15335 at 1212.844 kb on - strand, within BBR_RS15335
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,211,380 - BBR_RS15320 0.10 +0.6 1,211,504 - BBR_RS15320 0.56 +0.8 1,211,504 - BBR_RS15320 0.56 +0.4 1,211,510 - BBR_RS15320 0.58 -0.2 1,211,544 + BBR_RS15320 0.71 -0.1 1,211,552 - BBR_RS15320 0.74 +0.1 1,211,613 + +1.1 1,211,632 + +1.0 1,211,667 + -0.4 1,211,777 - +0.5 1,211,845 - -0.0 1,211,845 - -1.4 1,211,890 + BBR_RS15325 0.27 +0.6 1,211,912 - BBR_RS15325 0.39 +0.6 1,211,943 + BBR_RS15325 0.56 -1.7 1,211,943 + BBR_RS15325 0.56 +0.2 1,211,965 - BBR_RS15325 0.68 -0.1 1,211,983 - BBR_RS15325 0.77 -0.9 1,211,983 - BBR_RS15325 0.77 +2.2 1,211,996 - BBR_RS15325 0.84 +0.7 1,212,041 + -0.6 1,212,079 + +0.6 1,212,079 + +0.5 1,212,079 + -0.6 1,212,180 - BBR_RS15330 0.28 +0.4 1,212,180 - BBR_RS15330 0.28 +0.4 1,212,226 - BBR_RS15330 0.37 +0.2 1,212,311 + BBR_RS15330 0.53 -0.2 1,212,338 - BBR_RS15330 0.58 +0.6 1,212,346 - BBR_RS15330 0.60 +0.5 1,212,553 + +0.8 1,212,554 + -0.8 1,212,562 - -0.4 1,212,697 + BBR_RS15335 0.16 +0.4 1,212,706 + BBR_RS15335 0.17 +0.4 1,212,793 - BBR_RS15335 0.29 +0.2 1,212,836 + BBR_RS15335 0.35 -0.3 1,212,844 - BBR_RS15335 0.36 -0.2
Or see this region's nucleotide sequence