Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15285 and BBR_RS15290 are separated by 193 nucleotides BBR_RS15290 and BBR_RS15295 are separated by 95 nucleotides
BBR_RS15285: BBR_RS15285 - glycogen synthase, at 1,202,599 to 1,203,849
_RS15285
BBR_RS15290: BBR_RS15290 - ABC transporter ATP-binding protein, at 1,204,043 to 1,204,891
_RS15290
BBR_RS15295: BBR_RS15295 - Cof-type HAD-IIB family hydrolase, at 1,204,987 to 1,205,832
_RS15295
Position (kb)
1204
1205 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1203.879 kb on + strand at 1203.924 kb on - strand at 1203.950 kb on - strand at 1203.962 kb on + strand at 1204.045 kb on + strand at 1204.045 kb on + strand at 1204.074 kb on - strand at 1204.117 kb on - strand at 1204.145 kb on + strand, within BBR_RS15290 at 1204.145 kb on + strand, within BBR_RS15290 at 1204.145 kb on + strand, within BBR_RS15290 at 1204.169 kb on + strand, within BBR_RS15290 at 1204.212 kb on + strand, within BBR_RS15290 at 1204.263 kb on + strand, within BBR_RS15290 at 1204.334 kb on + strand, within BBR_RS15290 at 1204.363 kb on - strand, within BBR_RS15290 at 1204.395 kb on + strand, within BBR_RS15290 at 1204.411 kb on - strand, within BBR_RS15290 at 1204.477 kb on + strand, within BBR_RS15290 at 1204.485 kb on - strand, within BBR_RS15290 at 1204.519 kb on - strand, within BBR_RS15290 at 1204.545 kb on - strand, within BBR_RS15290 at 1204.563 kb on + strand, within BBR_RS15290 at 1204.563 kb on + strand, within BBR_RS15290 at 1204.563 kb on + strand, within BBR_RS15290 at 1204.571 kb on - strand, within BBR_RS15290 at 1204.571 kb on - strand, within BBR_RS15290 at 1204.571 kb on - strand, within BBR_RS15290 at 1204.589 kb on + strand, within BBR_RS15290 at 1204.599 kb on + strand, within BBR_RS15290 at 1204.642 kb on + strand, within BBR_RS15290 at 1204.730 kb on + strand, within BBR_RS15290 at 1204.736 kb on - strand, within BBR_RS15290 at 1204.777 kb on + strand, within BBR_RS15290 at 1204.782 kb on + strand, within BBR_RS15290 at 1204.810 kb on - strand at 1204.866 kb on + strand at 1204.926 kb on + strand at 1204.926 kb on + strand at 1204.926 kb on + strand at 1204.926 kb on + strand at 1204.926 kb on + strand at 1204.926 kb on + strand at 1204.932 kb on - strand at 1204.934 kb on - strand at 1204.934 kb on - strand at 1204.934 kb on - strand at 1204.935 kb on - strand at 1205.207 kb on - strand, within BBR_RS15295 at 1205.240 kb on + strand, within BBR_RS15295 at 1205.280 kb on + strand, within BBR_RS15295 at 1205.316 kb on - strand, within BBR_RS15295 at 1205.325 kb on - strand, within BBR_RS15295 at 1205.339 kb on + strand, within BBR_RS15295 at 1205.347 kb on + strand, within BBR_RS15295 at 1205.531 kb on + strand, within BBR_RS15295 at 1205.544 kb on + strand, within BBR_RS15295 at 1205.550 kb on - strand, within BBR_RS15295 at 1205.643 kb on + strand, within BBR_RS15295 at 1205.725 kb on + strand, within BBR_RS15295 at 1205.733 kb on - strand, within BBR_RS15295 at 1205.757 kb on - strand at 1205.757 kb on - strand at 1205.766 kb on + strand at 1205.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,203,879 + -0.0 1,203,924 - -0.0 1,203,950 - +0.5 1,203,962 + +0.4 1,204,045 + -0.2 1,204,045 + -0.2 1,204,074 - -0.2 1,204,117 - +0.5 1,204,145 + BBR_RS15290 0.12 +0.4 1,204,145 + BBR_RS15290 0.12 +2.2 1,204,145 + BBR_RS15290 0.12 +1.7 1,204,169 + BBR_RS15290 0.15 -0.2 1,204,212 + BBR_RS15290 0.20 +1.2 1,204,263 + BBR_RS15290 0.26 -0.2 1,204,334 + BBR_RS15290 0.34 -2.3 1,204,363 - BBR_RS15290 0.38 -0.1 1,204,395 + BBR_RS15290 0.41 +0.6 1,204,411 - BBR_RS15290 0.43 -0.2 1,204,477 + BBR_RS15290 0.51 +0.0 1,204,485 - BBR_RS15290 0.52 -1.5 1,204,519 - BBR_RS15290 0.56 +1.4 1,204,545 - BBR_RS15290 0.59 +1.8 1,204,563 + BBR_RS15290 0.61 -0.1 1,204,563 + BBR_RS15290 0.61 +0.8 1,204,563 + BBR_RS15290 0.61 +0.0 1,204,571 - BBR_RS15290 0.62 +0.0 1,204,571 - BBR_RS15290 0.62 +0.2 1,204,571 - BBR_RS15290 0.62 +0.1 1,204,589 + BBR_RS15290 0.64 +0.3 1,204,599 + BBR_RS15290 0.65 +0.6 1,204,642 + BBR_RS15290 0.71 -1.2 1,204,730 + BBR_RS15290 0.81 +0.4 1,204,736 - BBR_RS15290 0.82 -0.5 1,204,777 + BBR_RS15290 0.86 +0.3 1,204,782 + BBR_RS15290 0.87 +0.7 1,204,810 - +1.0 1,204,866 + -0.3 1,204,926 + +0.2 1,204,926 + +0.2 1,204,926 + +0.9 1,204,926 + +1.5 1,204,926 + -0.3 1,204,926 + +1.1 1,204,932 - -0.1 1,204,934 - +0.0 1,204,934 - -1.0 1,204,934 - +1.6 1,204,935 - -0.3 1,205,207 - BBR_RS15295 0.26 +1.0 1,205,240 + BBR_RS15295 0.30 +0.7 1,205,280 + BBR_RS15295 0.35 +0.5 1,205,316 - BBR_RS15295 0.39 +0.8 1,205,325 - BBR_RS15295 0.40 -0.1 1,205,339 + BBR_RS15295 0.42 -0.1 1,205,347 + BBR_RS15295 0.43 -1.7 1,205,531 + BBR_RS15295 0.64 -0.1 1,205,544 + BBR_RS15295 0.66 +0.2 1,205,550 - BBR_RS15295 0.67 +0.3 1,205,643 + BBR_RS15295 0.78 +0.4 1,205,725 + BBR_RS15295 0.87 -0.4 1,205,733 - BBR_RS15295 0.88 +1.2 1,205,757 - -0.0 1,205,757 - -0.5 1,205,766 + -0.1 1,205,766 - -0.6
Or see this region's nucleotide sequence