Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15275 and BBR_RS15280 are separated by 107 nucleotides BBR_RS15280 and BBR_RS15285 are separated by 128 nucleotides
BBR_RS15275: BBR_RS15275 - hypothetical protein, at 1,200,003 to 1,201,898
_RS15275
BBR_RS15280: BBR_RS15280 - hypothetical protein, at 1,202,006 to 1,202,470
_RS15280
BBR_RS15285: BBR_RS15285 - glycogen synthase, at 1,202,599 to 1,203,849
_RS15285
Position (kb)
1202
1203 Strain fitness (log2 ratio)
-2
-1
0
1 at 1201.109 kb on + strand, within BBR_RS15275 at 1201.109 kb on + strand, within BBR_RS15275 at 1201.126 kb on + strand, within BBR_RS15275 at 1201.126 kb on + strand, within BBR_RS15275 at 1201.174 kb on + strand, within BBR_RS15275 at 1201.178 kb on + strand, within BBR_RS15275 at 1201.205 kb on - strand, within BBR_RS15275 at 1201.285 kb on + strand, within BBR_RS15275 at 1201.317 kb on - strand, within BBR_RS15275 at 1201.317 kb on - strand, within BBR_RS15275 at 1201.333 kb on + strand, within BBR_RS15275 at 1201.371 kb on - strand, within BBR_RS15275 at 1201.414 kb on - strand, within BBR_RS15275 at 1201.441 kb on + strand, within BBR_RS15275 at 1201.446 kb on + strand, within BBR_RS15275 at 1201.461 kb on - strand, within BBR_RS15275 at 1201.461 kb on - strand, within BBR_RS15275 at 1201.517 kb on + strand, within BBR_RS15275 at 1201.519 kb on + strand, within BBR_RS15275 at 1201.519 kb on + strand, within BBR_RS15275 at 1201.535 kb on + strand, within BBR_RS15275 at 1201.541 kb on - strand, within BBR_RS15275 at 1201.543 kb on - strand, within BBR_RS15275 at 1201.602 kb on + strand, within BBR_RS15275 at 1201.611 kb on + strand, within BBR_RS15275 at 1201.623 kb on - strand, within BBR_RS15275 at 1201.756 kb on - strand at 1201.821 kb on + strand at 1201.844 kb on + strand at 1201.875 kb on - strand at 1202.016 kb on + strand at 1202.035 kb on + strand at 1202.045 kb on + strand at 1202.047 kb on + strand at 1202.047 kb on + strand at 1202.052 kb on + strand at 1202.052 kb on + strand at 1202.060 kb on - strand, within BBR_RS15280 at 1202.080 kb on - strand, within BBR_RS15280 at 1202.080 kb on - strand, within BBR_RS15280 at 1202.084 kb on + strand, within BBR_RS15280 at 1202.084 kb on + strand, within BBR_RS15280 at 1202.141 kb on + strand, within BBR_RS15280 at 1202.154 kb on - strand, within BBR_RS15280 at 1202.197 kb on + strand, within BBR_RS15280 at 1202.205 kb on - strand, within BBR_RS15280 at 1202.205 kb on - strand, within BBR_RS15280 at 1202.214 kb on + strand, within BBR_RS15280 at 1202.242 kb on - strand, within BBR_RS15280 at 1202.245 kb on + strand, within BBR_RS15280 at 1202.253 kb on - strand, within BBR_RS15280 at 1202.297 kb on - strand, within BBR_RS15280 at 1202.320 kb on - strand, within BBR_RS15280 at 1202.383 kb on + strand, within BBR_RS15280 at 1202.386 kb on + strand, within BBR_RS15280 at 1202.410 kb on + strand, within BBR_RS15280 at 1202.424 kb on + strand at 1202.550 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,201,109 + BBR_RS15275 0.58 -0.2 1,201,109 + BBR_RS15275 0.58 +0.8 1,201,126 + BBR_RS15275 0.59 +0.3 1,201,126 + BBR_RS15275 0.59 +1.3 1,201,174 + BBR_RS15275 0.62 -1.2 1,201,178 + BBR_RS15275 0.62 -1.8 1,201,205 - BBR_RS15275 0.63 +0.1 1,201,285 + BBR_RS15275 0.68 +0.2 1,201,317 - BBR_RS15275 0.69 -0.1 1,201,317 - BBR_RS15275 0.69 -0.4 1,201,333 + BBR_RS15275 0.70 -0.6 1,201,371 - BBR_RS15275 0.72 -0.0 1,201,414 - BBR_RS15275 0.74 +0.3 1,201,441 + BBR_RS15275 0.76 +1.5 1,201,446 + BBR_RS15275 0.76 -0.4 1,201,461 - BBR_RS15275 0.77 +0.3 1,201,461 - BBR_RS15275 0.77 +0.4 1,201,517 + BBR_RS15275 0.80 +0.2 1,201,519 + BBR_RS15275 0.80 -0.7 1,201,519 + BBR_RS15275 0.80 -0.4 1,201,535 + BBR_RS15275 0.81 +0.2 1,201,541 - BBR_RS15275 0.81 -0.4 1,201,543 - BBR_RS15275 0.81 +0.0 1,201,602 + BBR_RS15275 0.84 +0.2 1,201,611 + BBR_RS15275 0.85 -0.3 1,201,623 - BBR_RS15275 0.85 -0.3 1,201,756 - +0.8 1,201,821 + -1.9 1,201,844 + +0.8 1,201,875 - +0.5 1,202,016 + -0.2 1,202,035 + -2.1 1,202,045 + +0.5 1,202,047 + +0.3 1,202,047 + +0.4 1,202,052 + +0.2 1,202,052 + -0.1 1,202,060 - BBR_RS15280 0.12 -0.3 1,202,080 - BBR_RS15280 0.16 -0.6 1,202,080 - BBR_RS15280 0.16 +0.1 1,202,084 + BBR_RS15280 0.17 +0.2 1,202,084 + BBR_RS15280 0.17 +0.9 1,202,141 + BBR_RS15280 0.29 +0.9 1,202,154 - BBR_RS15280 0.32 +0.2 1,202,197 + BBR_RS15280 0.41 +0.2 1,202,205 - BBR_RS15280 0.43 -0.5 1,202,205 - BBR_RS15280 0.43 +0.1 1,202,214 + BBR_RS15280 0.45 +1.2 1,202,242 - BBR_RS15280 0.51 +0.0 1,202,245 + BBR_RS15280 0.51 -0.1 1,202,253 - BBR_RS15280 0.53 +0.1 1,202,297 - BBR_RS15280 0.63 +1.1 1,202,320 - BBR_RS15280 0.68 +1.1 1,202,383 + BBR_RS15280 0.81 +0.3 1,202,386 + BBR_RS15280 0.82 -0.1 1,202,410 + BBR_RS15280 0.87 -0.4 1,202,424 + -0.4 1,202,550 + +0.3
Or see this region's nucleotide sequence