Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15095 and BBR_RS15100 are separated by 160 nucleotides BBR_RS15100 and BBR_RS15105 are separated by 178 nucleotides
BBR_RS15095: BBR_RS15095 - CTP synthase, at 1,154,882 to 1,156,546
_RS15095
BBR_RS15100: BBR_RS15100 - murein L,D-transpeptidase, at 1,156,707 to 1,158,335
_RS15100
BBR_RS15105: BBR_RS15105 - Fe-S cluster assembly protein SufB, at 1,158,514 to 1,160,013
_RS15105
Position (kb)
1156
1157
1158
1159 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1156.507 kb on + strand at 1156.507 kb on + strand at 1156.515 kb on - strand at 1156.515 kb on - strand at 1156.542 kb on + strand at 1156.550 kb on - strand at 1156.576 kb on + strand at 1156.676 kb on - strand at 1156.701 kb on - strand at 1156.709 kb on - strand at 1156.797 kb on - strand at 1156.812 kb on - strand at 1156.816 kb on - strand at 1156.822 kb on - strand at 1156.890 kb on + strand, within BBR_RS15100 at 1156.902 kb on + strand, within BBR_RS15100 at 1156.969 kb on - strand, within BBR_RS15100 at 1156.974 kb on + strand, within BBR_RS15100 at 1156.974 kb on + strand, within BBR_RS15100 at 1156.986 kb on + strand, within BBR_RS15100 at 1156.987 kb on - strand, within BBR_RS15100 at 1157.050 kb on - strand, within BBR_RS15100 at 1157.097 kb on + strand, within BBR_RS15100 at 1157.097 kb on + strand, within BBR_RS15100 at 1157.199 kb on + strand, within BBR_RS15100 at 1157.216 kb on + strand, within BBR_RS15100 at 1157.224 kb on - strand, within BBR_RS15100 at 1157.237 kb on + strand, within BBR_RS15100 at 1157.237 kb on - strand, within BBR_RS15100 at 1157.251 kb on + strand, within BBR_RS15100 at 1157.310 kb on + strand, within BBR_RS15100 at 1157.323 kb on - strand, within BBR_RS15100 at 1157.341 kb on + strand, within BBR_RS15100 at 1157.345 kb on + strand, within BBR_RS15100 at 1157.353 kb on - strand, within BBR_RS15100 at 1157.397 kb on - strand, within BBR_RS15100 at 1157.409 kb on - strand, within BBR_RS15100 at 1157.425 kb on - strand, within BBR_RS15100 at 1157.439 kb on - strand, within BBR_RS15100 at 1157.508 kb on + strand, within BBR_RS15100 at 1157.518 kb on - strand, within BBR_RS15100 at 1157.571 kb on - strand, within BBR_RS15100 at 1157.638 kb on + strand, within BBR_RS15100 at 1157.638 kb on + strand, within BBR_RS15100 at 1157.672 kb on + strand, within BBR_RS15100 at 1157.672 kb on + strand, within BBR_RS15100 at 1157.710 kb on + strand, within BBR_RS15100 at 1157.712 kb on + strand, within BBR_RS15100 at 1157.772 kb on + strand, within BBR_RS15100 at 1157.772 kb on + strand, within BBR_RS15100 at 1157.782 kb on + strand, within BBR_RS15100 at 1157.782 kb on + strand, within BBR_RS15100 at 1157.782 kb on + strand, within BBR_RS15100 at 1157.788 kb on + strand, within BBR_RS15100 at 1157.840 kb on + strand, within BBR_RS15100 at 1157.871 kb on - strand, within BBR_RS15100 at 1157.874 kb on + strand, within BBR_RS15100 at 1157.878 kb on - strand, within BBR_RS15100 at 1157.881 kb on + strand, within BBR_RS15100 at 1157.881 kb on + strand, within BBR_RS15100 at 1157.997 kb on + strand, within BBR_RS15100 at 1158.016 kb on + strand, within BBR_RS15100 at 1158.016 kb on + strand, within BBR_RS15100 at 1158.020 kb on + strand, within BBR_RS15100 at 1158.024 kb on - strand, within BBR_RS15100 at 1158.028 kb on - strand, within BBR_RS15100 at 1158.028 kb on - strand, within BBR_RS15100 at 1158.028 kb on - strand, within BBR_RS15100 at 1158.072 kb on - strand, within BBR_RS15100 at 1158.073 kb on + strand, within BBR_RS15100 at 1158.073 kb on + strand, within BBR_RS15100 at 1158.073 kb on + strand, within BBR_RS15100 at 1158.091 kb on - strand, within BBR_RS15100 at 1158.097 kb on + strand, within BBR_RS15100 at 1158.097 kb on + strand, within BBR_RS15100 at 1158.097 kb on + strand, within BBR_RS15100 at 1158.124 kb on + strand, within BBR_RS15100 at 1158.134 kb on + strand, within BBR_RS15100 at 1158.204 kb on + strand at 1158.204 kb on + strand at 1158.269 kb on - strand at 1158.276 kb on + strand at 1158.296 kb on - strand at 1158.308 kb on + strand at 1158.310 kb on + strand at 1158.315 kb on + strand at 1158.316 kb on - strand at 1158.318 kb on - strand at 1158.318 kb on - strand at 1158.369 kb on + strand at 1158.387 kb on + strand at 1158.395 kb on - strand at 1158.405 kb on - strand at 1158.436 kb on - strand at 1158.449 kb on - strand at 1158.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,156,507 + -0.8 1,156,507 + -0.1 1,156,515 - +0.9 1,156,515 - -0.1 1,156,542 + -0.1 1,156,550 - +1.0 1,156,576 + +0.8 1,156,676 - +1.1 1,156,701 - +0.6 1,156,709 - +0.4 1,156,797 - +0.1 1,156,812 - +1.5 1,156,816 - +0.3 1,156,822 - +1.6 1,156,890 + BBR_RS15100 0.11 -0.4 1,156,902 + BBR_RS15100 0.12 +1.9 1,156,969 - BBR_RS15100 0.16 +0.3 1,156,974 + BBR_RS15100 0.16 +0.3 1,156,974 + BBR_RS15100 0.16 +0.1 1,156,986 + BBR_RS15100 0.17 -0.4 1,156,987 - BBR_RS15100 0.17 -0.2 1,157,050 - BBR_RS15100 0.21 -0.4 1,157,097 + BBR_RS15100 0.24 +0.4 1,157,097 + BBR_RS15100 0.24 +0.6 1,157,199 + BBR_RS15100 0.30 +0.6 1,157,216 + BBR_RS15100 0.31 -0.0 1,157,224 - BBR_RS15100 0.32 -0.7 1,157,237 + BBR_RS15100 0.33 -0.6 1,157,237 - BBR_RS15100 0.33 +0.4 1,157,251 + BBR_RS15100 0.33 +0.5 1,157,310 + BBR_RS15100 0.37 -0.9 1,157,323 - BBR_RS15100 0.38 +0.8 1,157,341 + BBR_RS15100 0.39 -0.3 1,157,345 + BBR_RS15100 0.39 +1.1 1,157,353 - BBR_RS15100 0.40 +0.3 1,157,397 - BBR_RS15100 0.42 +0.3 1,157,409 - BBR_RS15100 0.43 +1.0 1,157,425 - BBR_RS15100 0.44 -0.3 1,157,439 - BBR_RS15100 0.45 +0.2 1,157,508 + BBR_RS15100 0.49 -0.5 1,157,518 - BBR_RS15100 0.50 -1.0 1,157,571 - BBR_RS15100 0.53 -0.1 1,157,638 + BBR_RS15100 0.57 +0.1 1,157,638 + BBR_RS15100 0.57 +1.0 1,157,672 + BBR_RS15100 0.59 +0.0 1,157,672 + BBR_RS15100 0.59 +0.3 1,157,710 + BBR_RS15100 0.62 +0.1 1,157,712 + BBR_RS15100 0.62 -0.3 1,157,772 + BBR_RS15100 0.65 +0.3 1,157,772 + BBR_RS15100 0.65 +0.4 1,157,782 + BBR_RS15100 0.66 +0.1 1,157,782 + BBR_RS15100 0.66 +1.0 1,157,782 + BBR_RS15100 0.66 +0.0 1,157,788 + BBR_RS15100 0.66 +0.4 1,157,840 + BBR_RS15100 0.70 +0.2 1,157,871 - BBR_RS15100 0.71 +0.8 1,157,874 + BBR_RS15100 0.72 -0.1 1,157,878 - BBR_RS15100 0.72 +0.8 1,157,881 + BBR_RS15100 0.72 +0.2 1,157,881 + BBR_RS15100 0.72 +0.3 1,157,997 + BBR_RS15100 0.79 +1.6 1,158,016 + BBR_RS15100 0.80 -0.2 1,158,016 + BBR_RS15100 0.80 +0.3 1,158,020 + BBR_RS15100 0.81 +0.9 1,158,024 - BBR_RS15100 0.81 -0.1 1,158,028 - BBR_RS15100 0.81 +0.5 1,158,028 - BBR_RS15100 0.81 +0.5 1,158,028 - BBR_RS15100 0.81 +0.3 1,158,072 - BBR_RS15100 0.84 +1.6 1,158,073 + BBR_RS15100 0.84 +0.1 1,158,073 + BBR_RS15100 0.84 -0.2 1,158,073 + BBR_RS15100 0.84 +0.3 1,158,091 - BBR_RS15100 0.85 +0.2 1,158,097 + BBR_RS15100 0.85 +0.3 1,158,097 + BBR_RS15100 0.85 +0.4 1,158,097 + BBR_RS15100 0.85 +0.4 1,158,124 + BBR_RS15100 0.87 -0.4 1,158,134 + BBR_RS15100 0.88 +0.1 1,158,204 + +0.2 1,158,204 + -0.5 1,158,269 - -0.4 1,158,276 + +0.2 1,158,296 - +3.2 1,158,308 + +0.4 1,158,310 + +0.4 1,158,315 + +0.1 1,158,316 - +1.1 1,158,318 - +0.5 1,158,318 - +0.6 1,158,369 + -0.4 1,158,387 + -0.5 1,158,395 - -0.2 1,158,405 - -0.8 1,158,436 - -0.6 1,158,449 - +0.3 1,158,597 + -1.3
Or see this region's nucleotide sequence