Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14940 and BBR_RS14945 are separated by 193 nucleotides BBR_RS14945 and BBR_RS14950 are separated by 91 nucleotides BBR_RS14950 and BBR_RS14955 are separated by 109 nucleotides
BBR_RS14940: BBR_RS14940 - hypothetical protein, at 1,126,278 to 1,127,498
_RS14940
BBR_RS14945: BBR_RS14945 - peptidase E, at 1,127,692 to 1,128,315
_RS14945
BBR_RS14950: BBR_RS14950 - Pyrazinamidase/Nicotinamidase, at 1,128,407 to 1,129,021
_RS14950
BBR_RS14955: BBR_RS14955 - hypothetical protein, at 1,129,131 to 1,130,063
_RS14955
Position (kb)
1128
1129
1130 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1127.426 kb on - strand at 1127.440 kb on - strand at 1127.442 kb on - strand at 1127.442 kb on - strand at 1127.465 kb on + strand at 1127.465 kb on + strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.473 kb on - strand at 1127.476 kb on + strand at 1127.487 kb on + strand at 1127.497 kb on + strand at 1127.500 kb on - strand at 1127.558 kb on - strand at 1127.563 kb on + strand at 1127.566 kb on + strand at 1127.566 kb on - strand at 1127.573 kb on + strand at 1127.576 kb on - strand at 1127.592 kb on + strand at 1127.595 kb on - strand at 1127.632 kb on + strand at 1127.650 kb on + strand at 1127.658 kb on - strand at 1127.693 kb on - strand at 1127.771 kb on - strand, within BBR_RS14945 at 1127.776 kb on - strand, within BBR_RS14945 at 1127.801 kb on - strand, within BBR_RS14945 at 1127.803 kb on + strand, within BBR_RS14945 at 1127.805 kb on - strand, within BBR_RS14945 at 1127.811 kb on - strand, within BBR_RS14945 at 1127.851 kb on + strand, within BBR_RS14945 at 1127.859 kb on - strand, within BBR_RS14945 at 1127.859 kb on - strand, within BBR_RS14945 at 1127.874 kb on + strand, within BBR_RS14945 at 1127.881 kb on + strand, within BBR_RS14945 at 1127.904 kb on + strand, within BBR_RS14945 at 1127.920 kb on + strand, within BBR_RS14945 at 1127.954 kb on + strand, within BBR_RS14945 at 1127.956 kb on + strand, within BBR_RS14945 at 1128.006 kb on - strand, within BBR_RS14945 at 1128.072 kb on + strand, within BBR_RS14945 at 1128.151 kb on - strand, within BBR_RS14945 at 1128.164 kb on + strand, within BBR_RS14945 at 1128.206 kb on - strand, within BBR_RS14945 at 1128.214 kb on - strand, within BBR_RS14945 at 1128.286 kb on - strand at 1128.288 kb on + strand at 1128.320 kb on - strand at 1128.324 kb on + strand at 1128.348 kb on + strand at 1128.362 kb on - strand at 1128.390 kb on + strand at 1128.390 kb on + strand at 1128.422 kb on - strand at 1128.432 kb on - strand at 1128.472 kb on - strand, within BBR_RS14950 at 1128.511 kb on + strand, within BBR_RS14950 at 1128.519 kb on - strand, within BBR_RS14950 at 1128.545 kb on + strand, within BBR_RS14950 at 1128.575 kb on + strand, within BBR_RS14950 at 1128.583 kb on - strand, within BBR_RS14950 at 1128.612 kb on + strand, within BBR_RS14950 at 1128.612 kb on + strand, within BBR_RS14950 at 1128.613 kb on - strand, within BBR_RS14950 at 1128.618 kb on - strand, within BBR_RS14950 at 1128.625 kb on - strand, within BBR_RS14950 at 1128.678 kb on + strand, within BBR_RS14950 at 1128.694 kb on + strand, within BBR_RS14950 at 1128.694 kb on + strand, within BBR_RS14950 at 1128.757 kb on - strand, within BBR_RS14950 at 1128.837 kb on + strand, within BBR_RS14950 at 1128.850 kb on + strand, within BBR_RS14950 at 1128.864 kb on + strand, within BBR_RS14950 at 1128.872 kb on - strand, within BBR_RS14950 at 1128.964 kb on + strand at 1129.009 kb on + strand at 1129.012 kb on - strand at 1129.045 kb on + strand at 1129.053 kb on - strand at 1129.082 kb on + strand at 1129.090 kb on - strand at 1129.129 kb on + strand at 1129.129 kb on + strand at 1129.155 kb on - strand at 1129.244 kb on - strand, within BBR_RS14955 at 1129.263 kb on + strand, within BBR_RS14955 at 1129.271 kb on - strand, within BBR_RS14955 at 1129.271 kb on - strand, within BBR_RS14955 at 1129.271 kb on - strand, within BBR_RS14955 at 1129.271 kb on - strand, within BBR_RS14955 at 1129.319 kb on + strand, within BBR_RS14955 at 1129.319 kb on + strand, within BBR_RS14955 at 1129.327 kb on - strand, within BBR_RS14955 at 1129.341 kb on - strand, within BBR_RS14955 at 1129.341 kb on - strand, within BBR_RS14955 at 1129.364 kb on + strand, within BBR_RS14955 at 1129.423 kb on + strand, within BBR_RS14955 at 1129.423 kb on + strand, within BBR_RS14955 at 1129.507 kb on + strand, within BBR_RS14955 at 1129.507 kb on + strand, within BBR_RS14955 at 1129.507 kb on + strand, within BBR_RS14955 at 1129.507 kb on + strand, within BBR_RS14955 at 1129.515 kb on - strand, within BBR_RS14955 at 1129.515 kb on - strand, within BBR_RS14955 at 1129.515 kb on - strand, within BBR_RS14955 at 1129.552 kb on - strand, within BBR_RS14955 at 1129.552 kb on - strand, within BBR_RS14955 at 1129.552 kb on - strand, within BBR_RS14955 at 1129.638 kb on + strand, within BBR_RS14955 at 1129.677 kb on + strand, within BBR_RS14955 at 1129.709 kb on + strand, within BBR_RS14955 at 1129.732 kb on + strand, within BBR_RS14955 at 1129.765 kb on + strand, within BBR_RS14955 at 1129.802 kb on - strand, within BBR_RS14955 at 1129.833 kb on - strand, within BBR_RS14955 at 1129.833 kb on - strand, within BBR_RS14955 at 1129.840 kb on + strand, within BBR_RS14955 at 1129.861 kb on + strand, within BBR_RS14955
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,127,426 - +0.2 1,127,440 - -0.6 1,127,442 - -0.6 1,127,442 - -0.5 1,127,465 + +0.5 1,127,465 + +0.7 1,127,473 - -1.1 1,127,473 - +0.5 1,127,473 - +0.6 1,127,473 - +1.2 1,127,473 - -0.2 1,127,473 - -0.5 1,127,476 + +1.0 1,127,487 + -0.7 1,127,497 + +0.3 1,127,500 - +0.2 1,127,558 - +0.8 1,127,563 + +1.0 1,127,566 + +0.8 1,127,566 - +0.3 1,127,573 + +0.5 1,127,576 - +0.2 1,127,592 + -0.1 1,127,595 - -0.2 1,127,632 + -0.3 1,127,650 + +0.5 1,127,658 - +0.4 1,127,693 - -0.1 1,127,771 - BBR_RS14945 0.13 +0.8 1,127,776 - BBR_RS14945 0.13 +0.2 1,127,801 - BBR_RS14945 0.17 +0.2 1,127,803 + BBR_RS14945 0.18 +1.2 1,127,805 - BBR_RS14945 0.18 +0.7 1,127,811 - BBR_RS14945 0.19 +0.1 1,127,851 + BBR_RS14945 0.25 +0.7 1,127,859 - BBR_RS14945 0.27 +0.6 1,127,859 - BBR_RS14945 0.27 +1.5 1,127,874 + BBR_RS14945 0.29 -0.4 1,127,881 + BBR_RS14945 0.30 +0.5 1,127,904 + BBR_RS14945 0.34 +1.3 1,127,920 + BBR_RS14945 0.37 -1.0 1,127,954 + BBR_RS14945 0.42 +0.2 1,127,956 + BBR_RS14945 0.42 +0.4 1,128,006 - BBR_RS14945 0.50 +0.5 1,128,072 + BBR_RS14945 0.61 +0.8 1,128,151 - BBR_RS14945 0.74 -0.5 1,128,164 + BBR_RS14945 0.76 +0.1 1,128,206 - BBR_RS14945 0.82 +0.8 1,128,214 - BBR_RS14945 0.84 +0.8 1,128,286 - +0.3 1,128,288 + +0.1 1,128,320 - +0.9 1,128,324 + +3.2 1,128,348 + +0.2 1,128,362 - +0.9 1,128,390 + +0.0 1,128,390 + +0.2 1,128,422 - +0.2 1,128,432 - +0.1 1,128,472 - BBR_RS14950 0.11 +0.6 1,128,511 + BBR_RS14950 0.17 -0.1 1,128,519 - BBR_RS14950 0.18 +0.1 1,128,545 + BBR_RS14950 0.22 +0.6 1,128,575 + BBR_RS14950 0.27 +0.8 1,128,583 - BBR_RS14950 0.29 +1.2 1,128,612 + BBR_RS14950 0.33 -0.0 1,128,612 + BBR_RS14950 0.33 +0.2 1,128,613 - BBR_RS14950 0.33 -0.0 1,128,618 - BBR_RS14950 0.34 +1.4 1,128,625 - BBR_RS14950 0.35 -0.0 1,128,678 + BBR_RS14950 0.44 +0.4 1,128,694 + BBR_RS14950 0.47 +0.9 1,128,694 + BBR_RS14950 0.47 -0.3 1,128,757 - BBR_RS14950 0.57 +1.1 1,128,837 + BBR_RS14950 0.70 +0.3 1,128,850 + BBR_RS14950 0.72 -0.2 1,128,864 + BBR_RS14950 0.74 -2.6 1,128,872 - BBR_RS14950 0.76 -0.2 1,128,964 + -0.5 1,129,009 + +0.0 1,129,012 - +0.3 1,129,045 + +0.8 1,129,053 - +1.2 1,129,082 + -0.2 1,129,090 - +0.3 1,129,129 + -0.2 1,129,129 + -0.2 1,129,155 - -0.2 1,129,244 - BBR_RS14955 0.12 -0.6 1,129,263 + BBR_RS14955 0.14 +0.0 1,129,271 - BBR_RS14955 0.15 -0.1 1,129,271 - BBR_RS14955 0.15 +0.1 1,129,271 - BBR_RS14955 0.15 +1.1 1,129,271 - BBR_RS14955 0.15 +0.2 1,129,319 + BBR_RS14955 0.20 -1.9 1,129,319 + BBR_RS14955 0.20 -0.2 1,129,327 - BBR_RS14955 0.21 +0.0 1,129,341 - BBR_RS14955 0.23 +0.1 1,129,341 - BBR_RS14955 0.23 +0.3 1,129,364 + BBR_RS14955 0.25 -0.4 1,129,423 + BBR_RS14955 0.31 -0.1 1,129,423 + BBR_RS14955 0.31 +0.2 1,129,507 + BBR_RS14955 0.40 +0.6 1,129,507 + BBR_RS14955 0.40 -0.4 1,129,507 + BBR_RS14955 0.40 +0.5 1,129,507 + BBR_RS14955 0.40 -0.4 1,129,515 - BBR_RS14955 0.41 +0.3 1,129,515 - BBR_RS14955 0.41 -0.5 1,129,515 - BBR_RS14955 0.41 +0.5 1,129,552 - BBR_RS14955 0.45 +0.3 1,129,552 - BBR_RS14955 0.45 -1.0 1,129,552 - BBR_RS14955 0.45 +0.6 1,129,638 + BBR_RS14955 0.54 -0.3 1,129,677 + BBR_RS14955 0.59 -1.1 1,129,709 + BBR_RS14955 0.62 +0.2 1,129,732 + BBR_RS14955 0.64 +0.2 1,129,765 + BBR_RS14955 0.68 -0.2 1,129,802 - BBR_RS14955 0.72 -0.7 1,129,833 - BBR_RS14955 0.75 +0.1 1,129,833 - BBR_RS14955 0.75 -0.8 1,129,840 + BBR_RS14955 0.76 -1.0 1,129,861 + BBR_RS14955 0.78 -0.2
Or see this region's nucleotide sequence