Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14945

Experiment: Recovery broth with Isovaleric 75 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14940 and BBR_RS14945 are separated by 193 nucleotidesBBR_RS14945 and BBR_RS14950 are separated by 91 nucleotidesBBR_RS14950 and BBR_RS14955 are separated by 109 nucleotides BBR_RS14940: BBR_RS14940 - hypothetical protein, at 1,126,278 to 1,127,498 _RS14940 BBR_RS14945: BBR_RS14945 - peptidase E, at 1,127,692 to 1,128,315 _RS14945 BBR_RS14950: BBR_RS14950 - Pyrazinamidase/Nicotinamidase, at 1,128,407 to 1,129,021 _RS14950 BBR_RS14955: BBR_RS14955 - hypothetical protein, at 1,129,131 to 1,130,063 _RS14955 Position (kb) 1127 1128 1129Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1126.799 kb on - strand, within BBR_RS14940at 1126.811 kb on + strand, within BBR_RS14940at 1126.895 kb on + strand, within BBR_RS14940at 1126.957 kb on - strand, within BBR_RS14940at 1127.040 kb on - strand, within BBR_RS14940at 1127.040 kb on - strand, within BBR_RS14940at 1127.128 kb on + strand, within BBR_RS14940at 1127.263 kb on + strand, within BBR_RS14940at 1127.271 kb on - strand, within BBR_RS14940at 1127.271 kb on - strand, within BBR_RS14940at 1127.276 kb on + strand, within BBR_RS14940at 1127.325 kb on - strand, within BBR_RS14940at 1127.325 kb on - strand, within BBR_RS14940at 1127.343 kb on + strand, within BBR_RS14940at 1127.348 kb on - strand, within BBR_RS14940at 1127.403 kb on - strandat 1127.426 kb on - strandat 1127.440 kb on - strandat 1127.442 kb on - strandat 1127.442 kb on - strandat 1127.465 kb on + strandat 1127.465 kb on + strandat 1127.473 kb on - strandat 1127.473 kb on - strandat 1127.473 kb on - strandat 1127.473 kb on - strandat 1127.473 kb on - strandat 1127.473 kb on - strandat 1127.476 kb on + strandat 1127.487 kb on + strandat 1127.497 kb on + strandat 1127.500 kb on - strandat 1127.558 kb on - strandat 1127.563 kb on + strandat 1127.566 kb on + strandat 1127.566 kb on - strandat 1127.573 kb on + strandat 1127.576 kb on - strandat 1127.592 kb on + strandat 1127.595 kb on - strandat 1127.632 kb on + strandat 1127.650 kb on + strandat 1127.658 kb on - strandat 1127.693 kb on - strandat 1127.771 kb on - strand, within BBR_RS14945at 1127.776 kb on - strand, within BBR_RS14945at 1127.801 kb on - strand, within BBR_RS14945at 1127.803 kb on + strand, within BBR_RS14945at 1127.805 kb on - strand, within BBR_RS14945at 1127.811 kb on - strand, within BBR_RS14945at 1127.851 kb on + strand, within BBR_RS14945at 1127.859 kb on - strand, within BBR_RS14945at 1127.859 kb on - strand, within BBR_RS14945at 1127.874 kb on + strand, within BBR_RS14945at 1127.881 kb on + strand, within BBR_RS14945at 1127.904 kb on + strand, within BBR_RS14945at 1127.920 kb on + strand, within BBR_RS14945at 1127.954 kb on + strand, within BBR_RS14945at 1127.956 kb on + strand, within BBR_RS14945at 1128.006 kb on - strand, within BBR_RS14945at 1128.072 kb on + strand, within BBR_RS14945at 1128.151 kb on - strand, within BBR_RS14945at 1128.164 kb on + strand, within BBR_RS14945at 1128.206 kb on - strand, within BBR_RS14945at 1128.214 kb on - strand, within BBR_RS14945at 1128.286 kb on - strandat 1128.288 kb on + strandat 1128.320 kb on - strandat 1128.324 kb on + strandat 1128.348 kb on + strandat 1128.362 kb on - strandat 1128.390 kb on + strandat 1128.390 kb on + strandat 1128.422 kb on - strandat 1128.432 kb on - strandat 1128.472 kb on - strand, within BBR_RS14950at 1128.511 kb on + strand, within BBR_RS14950at 1128.519 kb on - strand, within BBR_RS14950at 1128.545 kb on + strand, within BBR_RS14950at 1128.575 kb on + strand, within BBR_RS14950at 1128.583 kb on - strand, within BBR_RS14950at 1128.612 kb on + strand, within BBR_RS14950at 1128.612 kb on + strand, within BBR_RS14950at 1128.613 kb on - strand, within BBR_RS14950at 1128.618 kb on - strand, within BBR_RS14950at 1128.625 kb on - strand, within BBR_RS14950at 1128.678 kb on + strand, within BBR_RS14950at 1128.694 kb on + strand, within BBR_RS14950at 1128.694 kb on + strand, within BBR_RS14950at 1128.757 kb on - strand, within BBR_RS14950at 1128.837 kb on + strand, within BBR_RS14950at 1128.850 kb on + strand, within BBR_RS14950at 1128.864 kb on + strand, within BBR_RS14950at 1128.872 kb on - strand, within BBR_RS14950at 1128.964 kb on + strandat 1129.009 kb on + strandat 1129.012 kb on - strandat 1129.045 kb on + strandat 1129.053 kb on - strandat 1129.082 kb on + strandat 1129.090 kb on - strandat 1129.129 kb on + strandat 1129.129 kb on + strandat 1129.155 kb on - strandat 1129.244 kb on - strand, within BBR_RS14955at 1129.263 kb on + strand, within BBR_RS14955at 1129.271 kb on - strand, within BBR_RS14955at 1129.271 kb on - strand, within BBR_RS14955at 1129.271 kb on - strand, within BBR_RS14955at 1129.271 kb on - strand, within BBR_RS14955

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM
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1,126,799 - BBR_RS14940 0.43 +1.2
1,126,811 + BBR_RS14940 0.44 -0.2
1,126,895 + BBR_RS14940 0.51 -0.4
1,126,957 - BBR_RS14940 0.56 -0.3
1,127,040 - BBR_RS14940 0.62 +0.0
1,127,040 - BBR_RS14940 0.62 +0.2
1,127,128 + BBR_RS14940 0.70 +0.3
1,127,263 + BBR_RS14940 0.81 +0.1
1,127,271 - BBR_RS14940 0.81 +0.4
1,127,271 - BBR_RS14940 0.81 +0.9
1,127,276 + BBR_RS14940 0.82 -0.4
1,127,325 - BBR_RS14940 0.86 -0.5
1,127,325 - BBR_RS14940 0.86 +2.2
1,127,343 + BBR_RS14940 0.87 +0.0
1,127,348 - BBR_RS14940 0.88 -0.4
1,127,403 - -0.6
1,127,426 - +0.2
1,127,440 - -0.6
1,127,442 - -0.6
1,127,442 - -0.5
1,127,465 + +0.5
1,127,465 + +0.7
1,127,473 - -1.1
1,127,473 - +0.5
1,127,473 - +0.6
1,127,473 - +1.2
1,127,473 - -0.2
1,127,473 - -0.5
1,127,476 + +1.0
1,127,487 + -0.7
1,127,497 + +0.3
1,127,500 - +0.2
1,127,558 - +0.8
1,127,563 + +1.0
1,127,566 + +0.8
1,127,566 - +0.3
1,127,573 + +0.5
1,127,576 - +0.2
1,127,592 + -0.1
1,127,595 - -0.2
1,127,632 + -0.3
1,127,650 + +0.5
1,127,658 - +0.4
1,127,693 - -0.1
1,127,771 - BBR_RS14945 0.13 +0.8
1,127,776 - BBR_RS14945 0.13 +0.2
1,127,801 - BBR_RS14945 0.17 +0.2
1,127,803 + BBR_RS14945 0.18 +1.2
1,127,805 - BBR_RS14945 0.18 +0.7
1,127,811 - BBR_RS14945 0.19 +0.1
1,127,851 + BBR_RS14945 0.25 +0.7
1,127,859 - BBR_RS14945 0.27 +0.6
1,127,859 - BBR_RS14945 0.27 +1.5
1,127,874 + BBR_RS14945 0.29 -0.4
1,127,881 + BBR_RS14945 0.30 +0.5
1,127,904 + BBR_RS14945 0.34 +1.3
1,127,920 + BBR_RS14945 0.37 -1.0
1,127,954 + BBR_RS14945 0.42 +0.2
1,127,956 + BBR_RS14945 0.42 +0.4
1,128,006 - BBR_RS14945 0.50 +0.5
1,128,072 + BBR_RS14945 0.61 +0.8
1,128,151 - BBR_RS14945 0.74 -0.5
1,128,164 + BBR_RS14945 0.76 +0.1
1,128,206 - BBR_RS14945 0.82 +0.8
1,128,214 - BBR_RS14945 0.84 +0.8
1,128,286 - +0.3
1,128,288 + +0.1
1,128,320 - +0.9
1,128,324 + +3.2
1,128,348 + +0.2
1,128,362 - +0.9
1,128,390 + +0.0
1,128,390 + +0.2
1,128,422 - +0.2
1,128,432 - +0.1
1,128,472 - BBR_RS14950 0.11 +0.6
1,128,511 + BBR_RS14950 0.17 -0.1
1,128,519 - BBR_RS14950 0.18 +0.1
1,128,545 + BBR_RS14950 0.22 +0.6
1,128,575 + BBR_RS14950 0.27 +0.8
1,128,583 - BBR_RS14950 0.29 +1.2
1,128,612 + BBR_RS14950 0.33 -0.0
1,128,612 + BBR_RS14950 0.33 +0.2
1,128,613 - BBR_RS14950 0.33 -0.0
1,128,618 - BBR_RS14950 0.34 +1.4
1,128,625 - BBR_RS14950 0.35 -0.0
1,128,678 + BBR_RS14950 0.44 +0.4
1,128,694 + BBR_RS14950 0.47 +0.9
1,128,694 + BBR_RS14950 0.47 -0.3
1,128,757 - BBR_RS14950 0.57 +1.1
1,128,837 + BBR_RS14950 0.70 +0.3
1,128,850 + BBR_RS14950 0.72 -0.2
1,128,864 + BBR_RS14950 0.74 -2.6
1,128,872 - BBR_RS14950 0.76 -0.2
1,128,964 + -0.5
1,129,009 + +0.0
1,129,012 - +0.3
1,129,045 + +0.8
1,129,053 - +1.2
1,129,082 + -0.2
1,129,090 - +0.3
1,129,129 + -0.2
1,129,129 + -0.2
1,129,155 - -0.2
1,129,244 - BBR_RS14955 0.12 -0.6
1,129,263 + BBR_RS14955 0.14 +0.0
1,129,271 - BBR_RS14955 0.15 -0.1
1,129,271 - BBR_RS14955 0.15 +0.1
1,129,271 - BBR_RS14955 0.15 +1.1
1,129,271 - BBR_RS14955 0.15 +0.2

Or see this region's nucleotide sequence