Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14465 and BBR_RS14470 are separated by 19 nucleotides BBR_RS14470 and BBR_RS14475 are separated by 274 nucleotides
BBR_RS14465: BBR_RS14465 - NAD+ synthase, at 1,018,768 to 1,020,465
_RS14465
BBR_RS14470: BBR_RS14470 - hypothetical protein, at 1,020,485 to 1,020,715
_RS14470
BBR_RS14475: BBR_RS14475 - formate C-acetyltransferase, at 1,020,990 to 1,023,365
_RS14475
Position (kb)
1020
1021 Strain fitness (log2 ratio)
-1
0
1 at 1020.511 kb on + strand, within BBR_RS14470 at 1020.534 kb on + strand, within BBR_RS14470 at 1020.542 kb on - strand, within BBR_RS14470 at 1020.570 kb on + strand, within BBR_RS14470 at 1020.576 kb on - strand, within BBR_RS14470 at 1020.578 kb on - strand, within BBR_RS14470 at 1020.586 kb on + strand, within BBR_RS14470 at 1020.613 kb on + strand, within BBR_RS14470 at 1020.618 kb on - strand, within BBR_RS14470 at 1020.618 kb on - strand, within BBR_RS14470 at 1020.620 kb on + strand, within BBR_RS14470 at 1020.623 kb on - strand, within BBR_RS14470 at 1020.634 kb on + strand, within BBR_RS14470 at 1020.763 kb on + strand at 1020.767 kb on + strand at 1020.775 kb on - strand at 1020.911 kb on + strand at 1020.911 kb on + strand at 1021.051 kb on + strand at 1021.084 kb on - strand at 1021.118 kb on + strand at 1021.125 kb on + strand at 1021.125 kb on + strand at 1021.198 kb on + strand at 1021.245 kb on - strand, within BBR_RS14475 at 1021.294 kb on - strand, within BBR_RS14475 at 1021.470 kb on + strand, within BBR_RS14475 at 1021.484 kb on + strand, within BBR_RS14475 at 1021.509 kb on + strand, within BBR_RS14475 at 1021.567 kb on + strand, within BBR_RS14475 at 1021.567 kb on + strand, within BBR_RS14475 at 1021.572 kb on + strand, within BBR_RS14475 at 1021.608 kb on - strand, within BBR_RS14475 at 1021.612 kb on + strand, within BBR_RS14475 at 1021.621 kb on + strand, within BBR_RS14475 at 1021.700 kb on + strand, within BBR_RS14475
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 1,020,511 + BBR_RS14470 0.11 -1.2 1,020,534 + BBR_RS14470 0.21 +0.9 1,020,542 - BBR_RS14470 0.25 +1.1 1,020,570 + BBR_RS14470 0.37 +0.6 1,020,576 - BBR_RS14470 0.39 +0.5 1,020,578 - BBR_RS14470 0.40 +1.2 1,020,586 + BBR_RS14470 0.44 +0.6 1,020,613 + BBR_RS14470 0.55 +0.4 1,020,618 - BBR_RS14470 0.58 -0.6 1,020,618 - BBR_RS14470 0.58 +0.2 1,020,620 + BBR_RS14470 0.58 +0.1 1,020,623 - BBR_RS14470 0.60 -0.5 1,020,634 + BBR_RS14470 0.65 -0.6 1,020,763 + +1.2 1,020,767 + +0.6 1,020,775 - +0.6 1,020,911 + -0.1 1,020,911 + -0.8 1,021,051 + +1.1 1,021,084 - +0.6 1,021,118 + -0.1 1,021,125 + -0.3 1,021,125 + +0.6 1,021,198 + -0.1 1,021,245 - BBR_RS14475 0.11 +0.2 1,021,294 - BBR_RS14475 0.13 +0.5 1,021,470 + BBR_RS14475 0.20 -0.5 1,021,484 + BBR_RS14475 0.21 +0.4 1,021,509 + BBR_RS14475 0.22 +0.0 1,021,567 + BBR_RS14475 0.24 +0.2 1,021,567 + BBR_RS14475 0.24 -0.5 1,021,572 + BBR_RS14475 0.24 -0.2 1,021,608 - BBR_RS14475 0.26 +0.3 1,021,612 + BBR_RS14475 0.26 +0.7 1,021,621 + BBR_RS14475 0.27 +0.4 1,021,700 + BBR_RS14475 0.30 -0.0
Or see this region's nucleotide sequence