Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14040

Experiment: Recovery broth with Isovaleric 75 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14030 and BBR_RS14035 overlap by 4 nucleotidesBBR_RS14035 and BBR_RS14040 are separated by 123 nucleotidesBBR_RS14040 and BBR_RS14045 are separated by 289 nucleotidesBBR_RS14045 and BBR_RS14050 are separated by 21 nucleotides BBR_RS14030: BBR_RS14030 - DNA repair protein RecN, at 924,030 to 925,880 _RS14030 BBR_RS14035: BBR_RS14035 - HAD family phosphatase, at 925,877 to 926,524 _RS14035 BBR_RS14040: BBR_RS14040 - GNAT family N-acetyltransferase, at 926,648 to 927,123 _RS14040 BBR_RS14045: BBR_RS14045 - GntR family transcriptional regulator, at 927,413 to 927,787 _RS14045 BBR_RS14050: BBR_RS14050 - ABC transporter ATP-binding protein, at 927,809 to 928,729 _RS14050 Position (kb) 926 927 928Strain fitness (log2 ratio) -2 -1 0 1 2at 925.703 kb on + strandat 925.712 kb on + strandat 925.804 kb on + strandat 925.822 kb on - strandat 925.836 kb on + strandat 925.844 kb on - strandat 925.887 kb on - strandat 925.970 kb on + strand, within BBR_RS14035at 926.007 kb on + strand, within BBR_RS14035at 926.015 kb on - strand, within BBR_RS14035at 926.033 kb on + strand, within BBR_RS14035at 926.041 kb on - strand, within BBR_RS14035at 926.043 kb on - strand, within BBR_RS14035at 926.043 kb on - strand, within BBR_RS14035at 926.057 kb on + strand, within BBR_RS14035at 926.075 kb on - strand, within BBR_RS14035at 926.106 kb on + strand, within BBR_RS14035at 926.182 kb on - strand, within BBR_RS14035at 926.190 kb on - strand, within BBR_RS14035at 926.223 kb on - strand, within BBR_RS14035at 926.312 kb on + strand, within BBR_RS14035at 926.315 kb on - strand, within BBR_RS14035at 926.344 kb on + strand, within BBR_RS14035at 926.359 kb on - strand, within BBR_RS14035at 926.366 kb on + strand, within BBR_RS14035at 926.385 kb on - strand, within BBR_RS14035at 926.385 kb on - strand, within BBR_RS14035at 926.412 kb on + strand, within BBR_RS14035at 926.419 kb on + strand, within BBR_RS14035at 926.420 kb on - strand, within BBR_RS14035at 926.420 kb on - strand, within BBR_RS14035at 926.424 kb on + strand, within BBR_RS14035at 926.427 kb on - strand, within BBR_RS14035at 926.455 kb on - strand, within BBR_RS14035at 926.508 kb on + strandat 926.532 kb on + strandat 926.541 kb on + strandat 926.619 kb on + strandat 926.639 kb on + strandat 926.642 kb on - strandat 926.664 kb on - strandat 926.731 kb on + strand, within BBR_RS14040at 926.814 kb on - strand, within BBR_RS14040at 926.814 kb on - strand, within BBR_RS14040at 926.852 kb on + strand, within BBR_RS14040at 926.977 kb on + strand, within BBR_RS14040at 926.987 kb on + strand, within BBR_RS14040at 926.987 kb on + strand, within BBR_RS14040at 926.992 kb on + strand, within BBR_RS14040at 926.995 kb on - strand, within BBR_RS14040at 927.019 kb on + strand, within BBR_RS14040at 927.031 kb on + strand, within BBR_RS14040at 927.058 kb on + strand, within BBR_RS14040at 927.058 kb on + strand, within BBR_RS14040at 927.192 kb on + strandat 927.197 kb on + strandat 927.223 kb on - strandat 927.229 kb on + strandat 927.237 kb on - strandat 927.237 kb on - strandat 927.412 kb on + strandat 927.437 kb on - strandat 927.438 kb on + strandat 927.487 kb on + strand, within BBR_RS14045at 927.555 kb on + strand, within BBR_RS14045at 927.555 kb on + strand, within BBR_RS14045at 927.608 kb on + strand, within BBR_RS14045at 927.673 kb on + strand, within BBR_RS14045at 927.673 kb on + strand, within BBR_RS14045at 927.746 kb on + strand, within BBR_RS14045at 927.746 kb on + strand, within BBR_RS14045at 927.747 kb on + strand, within BBR_RS14045at 927.747 kb on + strand, within BBR_RS14045at 927.747 kb on + strand, within BBR_RS14045at 927.781 kb on + strandat 927.858 kb on + strandat 927.868 kb on + strandat 927.899 kb on + strandat 927.909 kb on + strand, within BBR_RS14050at 927.924 kb on - strand, within BBR_RS14050at 927.957 kb on + strand, within BBR_RS14050at 927.967 kb on + strand, within BBR_RS14050at 927.967 kb on + strand, within BBR_RS14050at 927.984 kb on + strand, within BBR_RS14050at 928.033 kb on - strand, within BBR_RS14050at 928.082 kb on - strand, within BBR_RS14050at 928.102 kb on - strand, within BBR_RS14050at 928.109 kb on + strand, within BBR_RS14050

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM
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925,703 + +1.1
925,712 + -1.0
925,804 + -0.8
925,822 - +0.6
925,836 + +0.1
925,844 - +0.1
925,887 - +0.8
925,970 + BBR_RS14035 0.14 +0.1
926,007 + BBR_RS14035 0.20 -0.4
926,015 - BBR_RS14035 0.21 +0.3
926,033 + BBR_RS14035 0.24 -0.2
926,041 - BBR_RS14035 0.25 -0.1
926,043 - BBR_RS14035 0.26 -0.8
926,043 - BBR_RS14035 0.26 -1.9
926,057 + BBR_RS14035 0.28 +0.6
926,075 - BBR_RS14035 0.31 +0.4
926,106 + BBR_RS14035 0.35 -1.4
926,182 - BBR_RS14035 0.47 +0.1
926,190 - BBR_RS14035 0.48 +0.1
926,223 - BBR_RS14035 0.53 +0.8
926,312 + BBR_RS14035 0.67 -0.1
926,315 - BBR_RS14035 0.68 -0.3
926,344 + BBR_RS14035 0.72 +0.8
926,359 - BBR_RS14035 0.74 +1.4
926,366 + BBR_RS14035 0.75 -0.6
926,385 - BBR_RS14035 0.78 +0.8
926,385 - BBR_RS14035 0.78 +0.3
926,412 + BBR_RS14035 0.83 +0.4
926,419 + BBR_RS14035 0.84 -0.5
926,420 - BBR_RS14035 0.84 -0.3
926,420 - BBR_RS14035 0.84 +1.2
926,424 + BBR_RS14035 0.84 +0.2
926,427 - BBR_RS14035 0.85 -0.5
926,455 - BBR_RS14035 0.89 +0.8
926,508 + +0.2
926,532 + +0.3
926,541 + +1.2
926,619 + +0.2
926,639 + +0.7
926,642 - -0.4
926,664 - +0.1
926,731 + BBR_RS14040 0.17 +0.4
926,814 - BBR_RS14040 0.35 +0.2
926,814 - BBR_RS14040 0.35 +0.2
926,852 + BBR_RS14040 0.43 +0.6
926,977 + BBR_RS14040 0.69 +2.6
926,987 + BBR_RS14040 0.71 +0.9
926,987 + BBR_RS14040 0.71 -0.2
926,992 + BBR_RS14040 0.72 -0.1
926,995 - BBR_RS14040 0.73 +0.0
927,019 + BBR_RS14040 0.78 -0.9
927,031 + BBR_RS14040 0.80 -0.1
927,058 + BBR_RS14040 0.86 -1.0
927,058 + BBR_RS14040 0.86 -1.8
927,192 + -2.3
927,197 + -0.4
927,223 - -1.0
927,229 + -1.8
927,237 - -0.7
927,237 - -0.4
927,412 + -1.2
927,437 - -0.3
927,438 + +0.1
927,487 + BBR_RS14045 0.20 -0.6
927,555 + BBR_RS14045 0.38 -0.1
927,555 + BBR_RS14045 0.38 -0.2
927,608 + BBR_RS14045 0.52 +0.0
927,673 + BBR_RS14045 0.69 -0.4
927,673 + BBR_RS14045 0.69 -0.3
927,746 + BBR_RS14045 0.89 -0.8
927,746 + BBR_RS14045 0.89 -0.3
927,747 + BBR_RS14045 0.89 -0.2
927,747 + BBR_RS14045 0.89 +0.5
927,747 + BBR_RS14045 0.89 -0.0
927,781 + -0.5
927,858 + +0.5
927,868 + -0.9
927,899 + +0.6
927,909 + BBR_RS14050 0.11 +0.3
927,924 - BBR_RS14050 0.12 -0.2
927,957 + BBR_RS14050 0.16 +0.1
927,967 + BBR_RS14050 0.17 -0.9
927,967 + BBR_RS14050 0.17 -0.1
927,984 + BBR_RS14050 0.19 +0.1
928,033 - BBR_RS14050 0.24 -0.4
928,082 - BBR_RS14050 0.30 -0.6
928,102 - BBR_RS14050 0.32 +0.7
928,109 + BBR_RS14050 0.33 -0.6

Or see this region's nucleotide sequence