Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13925 and BBR_RS13930 are separated by 2 nucleotides BBR_RS13930 and BBR_RS13935 are separated by 91 nucleotides BBR_RS13935 and BBR_RS13940 are separated by 158 nucleotides BBR_RS13940 and BBR_RS13945 are separated by 168 nucleotides BBR_RS13945 and BBR_RS13950 are separated by 1 nucleotides
BBR_RS13925: BBR_RS13925 - IS21 family transposase, at 903,898 to 905,379
_RS13925
BBR_RS13930: BBR_RS13930 - ATPase AAA, at 905,382 to 906,167
_RS13930
BBR_RS13935: BBR_RS13935 - hypothetical protein, at 906,259 to 906,630
_RS13935
BBR_RS13940: BBR_RS13940 - integrase, at 906,789 to 907,040
_RS13940
BBR_RS13945: BBR_RS13945 - Txe/YoeB family addiction module toxin, at 907,209 to 907,484
_RS13945
BBR_RS13950: BBR_RS13950 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 907,486 to 907,740
_RS13950
Position (kb)
906
907 Strain fitness (log2 ratio)
-1
0
1 at 906.286 kb on + strand at 906.345 kb on + strand, within BBR_RS13935 at 906.372 kb on + strand, within BBR_RS13935 at 906.377 kb on + strand, within BBR_RS13935 at 906.385 kb on - strand, within BBR_RS13935 at 906.428 kb on - strand, within BBR_RS13935 at 906.453 kb on + strand, within BBR_RS13935 at 906.468 kb on - strand, within BBR_RS13935 at 906.489 kb on + strand, within BBR_RS13935 at 906.494 kb on + strand, within BBR_RS13935 at 906.723 kb on - strand at 906.724 kb on + strand at 906.724 kb on + strand at 906.806 kb on + strand at 906.835 kb on - strand, within BBR_RS13940 at 906.892 kb on + strand, within BBR_RS13940 at 906.895 kb on - strand, within BBR_RS13940 at 906.935 kb on - strand, within BBR_RS13940 at 906.935 kb on - strand, within BBR_RS13940 at 906.989 kb on + strand, within BBR_RS13940 at 907.005 kb on + strand, within BBR_RS13940 at 907.072 kb on - strand at 907.078 kb on - strand at 907.078 kb on - strand at 907.153 kb on - strand at 907.155 kb on - strand at 907.158 kb on - strand at 907.158 kb on - strand at 907.174 kb on - strand at 907.183 kb on + strand at 907.184 kb on - strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.214 kb on + strand at 907.215 kb on - strand at 907.215 kb on - strand at 907.234 kb on - strand at 907.307 kb on + strand, within BBR_RS13945 at 907.350 kb on - strand, within BBR_RS13945 at 907.360 kb on - strand, within BBR_RS13945 at 907.434 kb on + strand, within BBR_RS13945 at 907.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 906,286 + -0.5 906,345 + BBR_RS13935 0.23 +0.6 906,372 + BBR_RS13935 0.30 +0.5 906,377 + BBR_RS13935 0.32 +0.9 906,385 - BBR_RS13935 0.34 +0.4 906,428 - BBR_RS13935 0.45 +0.3 906,453 + BBR_RS13935 0.52 +0.5 906,468 - BBR_RS13935 0.56 +0.7 906,489 + BBR_RS13935 0.62 +0.5 906,494 + BBR_RS13935 0.63 +0.2 906,723 - +0.4 906,724 + -0.1 906,724 + +0.1 906,806 + -0.7 906,835 - BBR_RS13940 0.18 -0.8 906,892 + BBR_RS13940 0.41 -0.0 906,895 - BBR_RS13940 0.42 -0.8 906,935 - BBR_RS13940 0.58 +0.1 906,935 - BBR_RS13940 0.58 -0.1 906,989 + BBR_RS13940 0.79 +0.5 907,005 + BBR_RS13940 0.86 +0.2 907,072 - +0.5 907,078 - -0.2 907,078 - +0.1 907,153 - -0.1 907,155 - -0.2 907,158 - -0.3 907,158 - -0.6 907,174 - +0.1 907,183 + +0.6 907,184 - -0.2 907,207 + +0.0 907,207 + -0.2 907,207 + +0.4 907,207 + -0.3 907,214 + +0.3 907,215 - -0.5 907,215 - +0.8 907,234 - +0.8 907,307 + BBR_RS13945 0.36 -0.9 907,350 - BBR_RS13945 0.51 +0.2 907,360 - BBR_RS13945 0.55 +0.6 907,434 + BBR_RS13945 0.82 -0.9 907,479 + -0.5
Or see this region's nucleotide sequence