Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13850 and BBR_RS13855 overlap by 1 nucleotides BBR_RS13855 and BBR_RS13860 are separated by 56 nucleotides BBR_RS13860 and BBR_RS13865 are separated by 36 nucleotides
BBR_RS13850: BBR_RS13850 - ABC transporter permease, at 886,465 to 887,064
_RS13850
BBR_RS13855: BBR_RS13855 - ABC transporter ATP-binding protein, at 887,064 to 888,536
_RS13855
BBR_RS13860: BBR_RS13860 - energy-coupling factor transporter transmembrane protein EcfT, at 888,593 to 889,471
_RS13860
BBR_RS13865: BBR_RS13865 - RNA methyltransferase, at 889,508 to 890,386
_RS13865
Position (kb)
887
888
889 Strain fitness (log2 ratio)
-1
0
1
2 at 886.090 kb on + strand at 886.123 kb on + strand at 886.275 kb on - strand at 886.275 kb on - strand at 886.283 kb on - strand at 886.290 kb on - strand at 886.298 kb on - strand at 886.332 kb on - strand at 886.342 kb on + strand at 886.359 kb on - strand at 886.359 kb on - strand at 886.406 kb on + strand at 886.427 kb on + strand at 886.427 kb on + strand at 886.461 kb on + strand at 886.561 kb on + strand, within BBR_RS13850 at 886.572 kb on + strand, within BBR_RS13850 at 886.573 kb on + strand, within BBR_RS13850 at 886.590 kb on + strand, within BBR_RS13850 at 886.688 kb on + strand, within BBR_RS13850 at 886.691 kb on - strand, within BBR_RS13850 at 886.722 kb on + strand, within BBR_RS13850 at 886.751 kb on - strand, within BBR_RS13850 at 886.798 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.844 kb on + strand, within BBR_RS13850 at 886.885 kb on + strand, within BBR_RS13850 at 886.890 kb on + strand, within BBR_RS13850 at 886.954 kb on - strand, within BBR_RS13850 at 887.066 kb on - strand at 887.069 kb on + strand at 887.166 kb on + strand at 887.173 kb on + strand at 887.278 kb on + strand, within BBR_RS13855 at 887.312 kb on - strand, within BBR_RS13855 at 887.312 kb on - strand, within BBR_RS13855 at 887.336 kb on - strand, within BBR_RS13855 at 887.379 kb on + strand, within BBR_RS13855 at 887.474 kb on + strand, within BBR_RS13855 at 887.545 kb on + strand, within BBR_RS13855 at 887.646 kb on - strand, within BBR_RS13855 at 887.690 kb on - strand, within BBR_RS13855 at 887.767 kb on - strand, within BBR_RS13855 at 887.856 kb on + strand, within BBR_RS13855 at 887.899 kb on + strand, within BBR_RS13855 at 887.907 kb on - strand, within BBR_RS13855 at 887.911 kb on + strand, within BBR_RS13855 at 887.972 kb on - strand, within BBR_RS13855 at 887.973 kb on + strand, within BBR_RS13855 at 887.975 kb on + strand, within BBR_RS13855 at 887.975 kb on + strand, within BBR_RS13855 at 888.008 kb on + strand, within BBR_RS13855 at 888.023 kb on + strand, within BBR_RS13855 at 888.069 kb on - strand, within BBR_RS13855 at 888.090 kb on - strand, within BBR_RS13855 at 888.116 kb on + strand, within BBR_RS13855 at 888.137 kb on - strand, within BBR_RS13855 at 888.163 kb on + strand, within BBR_RS13855 at 888.226 kb on + strand, within BBR_RS13855 at 888.267 kb on + strand, within BBR_RS13855 at 888.310 kb on - strand, within BBR_RS13855 at 888.320 kb on + strand, within BBR_RS13855 at 888.328 kb on - strand, within BBR_RS13855 at 888.339 kb on + strand, within BBR_RS13855 at 888.342 kb on - strand, within BBR_RS13855 at 888.342 kb on - strand, within BBR_RS13855 at 888.354 kb on + strand, within BBR_RS13855 at 888.354 kb on + strand, within BBR_RS13855 at 888.360 kb on + strand, within BBR_RS13855 at 888.385 kb on - strand, within BBR_RS13855 at 888.446 kb on + strand at 888.454 kb on - strand at 888.456 kb on - strand at 888.473 kb on + strand at 888.485 kb on + strand at 888.493 kb on - strand at 888.538 kb on - strand at 888.560 kb on + strand at 888.568 kb on - strand at 888.666 kb on + strand at 888.691 kb on + strand, within BBR_RS13860 at 888.691 kb on + strand, within BBR_RS13860 at 888.691 kb on + strand, within BBR_RS13860 at 888.699 kb on - strand, within BBR_RS13860 at 888.758 kb on + strand, within BBR_RS13860 at 888.766 kb on - strand, within BBR_RS13860 at 888.786 kb on + strand, within BBR_RS13860 at 888.786 kb on + strand, within BBR_RS13860 at 888.843 kb on - strand, within BBR_RS13860 at 888.856 kb on + strand, within BBR_RS13860 at 888.856 kb on + strand, within BBR_RS13860 at 888.856 kb on + strand, within BBR_RS13860 at 888.864 kb on - strand, within BBR_RS13860 at 888.864 kb on - strand, within BBR_RS13860 at 888.864 kb on - strand, within BBR_RS13860 at 888.887 kb on + strand, within BBR_RS13860 at 888.933 kb on - strand, within BBR_RS13860 at 888.984 kb on - strand, within BBR_RS13860 at 888.995 kb on - strand, within BBR_RS13860 at 889.056 kb on + strand, within BBR_RS13860 at 889.101 kb on - strand, within BBR_RS13860 at 889.119 kb on + strand, within BBR_RS13860 at 889.153 kb on + strand, within BBR_RS13860 at 889.182 kb on + strand, within BBR_RS13860 at 889.190 kb on - strand, within BBR_RS13860 at 889.190 kb on - strand, within BBR_RS13860 at 889.192 kb on + strand, within BBR_RS13860 at 889.217 kb on - strand, within BBR_RS13860 at 889.219 kb on + strand, within BBR_RS13860 at 889.265 kb on + strand, within BBR_RS13860 at 889.311 kb on + strand, within BBR_RS13860 at 889.381 kb on + strand, within BBR_RS13860 at 889.381 kb on - strand, within BBR_RS13860 at 889.389 kb on + strand at 889.389 kb on - strand at 889.389 kb on - strand at 889.406 kb on - strand at 889.416 kb on + strand at 889.416 kb on + strand at 889.416 kb on + strand at 889.424 kb on - strand at 889.497 kb on + strand at 889.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 886,090 + +0.3 886,123 + -0.3 886,275 - +0.0 886,275 - +0.1 886,283 - +0.0 886,290 - -1.0 886,298 - +0.2 886,332 - -1.5 886,342 + -0.6 886,359 - -0.2 886,359 - -0.1 886,406 + +0.1 886,427 + +0.2 886,427 + +0.4 886,461 + -0.4 886,561 + BBR_RS13850 0.16 -0.3 886,572 + BBR_RS13850 0.18 -1.0 886,573 + BBR_RS13850 0.18 +0.2 886,590 + BBR_RS13850 0.21 -0.5 886,688 + BBR_RS13850 0.37 -0.4 886,691 - BBR_RS13850 0.38 +0.1 886,722 + BBR_RS13850 0.43 +0.4 886,751 - BBR_RS13850 0.48 -0.1 886,798 + BBR_RS13850 0.56 +0.5 886,844 + BBR_RS13850 0.63 +0.4 886,844 + BBR_RS13850 0.63 -0.5 886,844 + BBR_RS13850 0.63 +0.4 886,885 + BBR_RS13850 0.70 +1.8 886,890 + BBR_RS13850 0.71 +0.3 886,954 - BBR_RS13850 0.81 +1.1 887,066 - -0.3 887,069 + -0.6 887,166 + -0.4 887,173 + +0.3 887,278 + BBR_RS13855 0.15 +0.6 887,312 - BBR_RS13855 0.17 +1.2 887,312 - BBR_RS13855 0.17 -0.4 887,336 - BBR_RS13855 0.18 -0.8 887,379 + BBR_RS13855 0.21 +0.3 887,474 + BBR_RS13855 0.28 +1.0 887,545 + BBR_RS13855 0.33 -0.2 887,646 - BBR_RS13855 0.40 +0.5 887,690 - BBR_RS13855 0.42 +1.7 887,767 - BBR_RS13855 0.48 +0.0 887,856 + BBR_RS13855 0.54 -0.0 887,899 + BBR_RS13855 0.57 +1.3 887,907 - BBR_RS13855 0.57 -0.1 887,911 + BBR_RS13855 0.58 +0.1 887,972 - BBR_RS13855 0.62 -0.4 887,973 + BBR_RS13855 0.62 +1.3 887,975 + BBR_RS13855 0.62 +0.4 887,975 + BBR_RS13855 0.62 -0.5 888,008 + BBR_RS13855 0.64 +0.1 888,023 + BBR_RS13855 0.65 -0.3 888,069 - BBR_RS13855 0.68 +0.8 888,090 - BBR_RS13855 0.70 +1.0 888,116 + BBR_RS13855 0.71 -0.5 888,137 - BBR_RS13855 0.73 +0.3 888,163 + BBR_RS13855 0.75 -1.1 888,226 + BBR_RS13855 0.79 +0.5 888,267 + BBR_RS13855 0.82 -0.7 888,310 - BBR_RS13855 0.85 +0.3 888,320 + BBR_RS13855 0.85 +0.4 888,328 - BBR_RS13855 0.86 -0.1 888,339 + BBR_RS13855 0.87 +0.8 888,342 - BBR_RS13855 0.87 -0.5 888,342 - BBR_RS13855 0.87 +0.3 888,354 + BBR_RS13855 0.88 +0.1 888,354 + BBR_RS13855 0.88 -0.8 888,360 + BBR_RS13855 0.88 +0.4 888,385 - BBR_RS13855 0.90 -0.3 888,446 + -0.0 888,454 - +0.6 888,456 - +0.1 888,473 + -0.2 888,485 + +1.5 888,493 - -0.9 888,538 - +0.5 888,560 + +0.8 888,568 - +0.4 888,666 + +1.0 888,691 + BBR_RS13860 0.11 +0.4 888,691 + BBR_RS13860 0.11 -0.3 888,691 + BBR_RS13860 0.11 -0.1 888,699 - BBR_RS13860 0.12 +0.0 888,758 + BBR_RS13860 0.19 +0.3 888,766 - BBR_RS13860 0.20 +0.5 888,786 + BBR_RS13860 0.22 +0.6 888,786 + BBR_RS13860 0.22 +0.3 888,843 - BBR_RS13860 0.28 -0.0 888,856 + BBR_RS13860 0.30 +0.7 888,856 + BBR_RS13860 0.30 +0.5 888,856 + BBR_RS13860 0.30 -0.1 888,864 - BBR_RS13860 0.31 +0.3 888,864 - BBR_RS13860 0.31 +0.4 888,864 - BBR_RS13860 0.31 +1.1 888,887 + BBR_RS13860 0.33 -0.2 888,933 - BBR_RS13860 0.39 +0.1 888,984 - BBR_RS13860 0.44 -1.6 888,995 - BBR_RS13860 0.46 +0.8 889,056 + BBR_RS13860 0.53 -0.1 889,101 - BBR_RS13860 0.58 +0.5 889,119 + BBR_RS13860 0.60 +0.5 889,153 + BBR_RS13860 0.64 +0.7 889,182 + BBR_RS13860 0.67 -0.2 889,190 - BBR_RS13860 0.68 -1.1 889,190 - BBR_RS13860 0.68 +0.5 889,192 + BBR_RS13860 0.68 +0.2 889,217 - BBR_RS13860 0.71 -0.0 889,219 + BBR_RS13860 0.71 +0.7 889,265 + BBR_RS13860 0.76 -0.2 889,311 + BBR_RS13860 0.82 -0.3 889,381 + BBR_RS13860 0.90 -0.1 889,381 - BBR_RS13860 0.90 +0.8 889,389 + +0.1 889,389 - +0.0 889,389 - +0.3 889,406 - +1.0 889,416 + -0.2 889,416 + +0.1 889,416 + +0.2 889,424 - +0.5 889,497 + +0.4 889,510 + -0.2
Or see this region's nucleotide sequence