Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13530 and BBR_RS13535 are separated by 207 nucleotides BBR_RS13535 and BBR_RS13540 are separated by 40 nucleotides BBR_RS13540 and BBR_RS13545 are separated by 228 nucleotides
BBR_RS13530: BBR_RS13530 - CrcB family protein, at 807,481 to 808,017
_RS13530
BBR_RS13535: BBR_RS13535 - MerR family DNA-binding transcriptional regulator, at 808,225 to 808,689
_RS13535
BBR_RS13540: BBR_RS13540 - alcohol dehydrogenase, at 808,730 to 809,773
_RS13540
BBR_RS13545: BBR_RS13545 - DUF4143 domain-containing protein, at 810,002 to 811,363
_RS13545
Position (kb) 808
809
810 Strain fitness (log2 ratio) -1
0
1
2 at 807.734 kb on - strand, within BBR_RS13530 at 807.787 kb on + strand, within BBR_RS13530 at 807.792 kb on + strand, within BBR_RS13530 at 807.797 kb on + strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.933 kb on + strand, within BBR_RS13530 at 807.955 kb on - strand, within BBR_RS13530 at 807.957 kb on + strand, within BBR_RS13530 at 807.998 kb on + strand at 808.000 kb on - strand at 808.277 kb on + strand, within BBR_RS13535 at 808.301 kb on + strand, within BBR_RS13535 at 808.304 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.354 kb on - strand, within BBR_RS13535 at 808.386 kb on + strand, within BBR_RS13535 at 808.392 kb on - strand, within BBR_RS13535 at 808.394 kb on - strand, within BBR_RS13535 at 808.394 kb on - strand, within BBR_RS13535 at 808.439 kb on + strand, within BBR_RS13535 at 808.447 kb on - strand, within BBR_RS13535 at 808.465 kb on + strand, within BBR_RS13535 at 808.495 kb on - strand, within BBR_RS13535 at 808.495 kb on - strand, within BBR_RS13535 at 808.509 kb on + strand, within BBR_RS13535 at 808.525 kb on + strand, within BBR_RS13535 at 808.554 kb on + strand, within BBR_RS13535 at 808.574 kb on - strand, within BBR_RS13535 at 808.574 kb on - strand, within BBR_RS13535 at 808.595 kb on - strand, within BBR_RS13535 at 808.656 kb on + strand at 808.664 kb on - strand at 808.664 kb on - strand at 808.664 kb on - strand at 808.803 kb on + strand at 808.811 kb on - strand at 808.821 kb on - strand at 808.822 kb on - strand at 808.823 kb on + strand at 808.831 kb on - strand at 808.838 kb on + strand, within BBR_RS13540 at 808.838 kb on + strand, within BBR_RS13540 at 808.846 kb on - strand, within BBR_RS13540 at 808.875 kb on + strand, within BBR_RS13540 at 808.883 kb on - strand, within BBR_RS13540 at 808.895 kb on + strand, within BBR_RS13540 at 808.895 kb on + strand, within BBR_RS13540 at 808.921 kb on + strand, within BBR_RS13540 at 808.924 kb on + strand, within BBR_RS13540 at 808.937 kb on - strand, within BBR_RS13540 at 809.088 kb on + strand, within BBR_RS13540 at 809.108 kb on + strand, within BBR_RS13540 at 809.120 kb on - strand, within BBR_RS13540 at 809.156 kb on - strand, within BBR_RS13540 at 809.184 kb on - strand, within BBR_RS13540 at 809.210 kb on - strand, within BBR_RS13540 at 809.227 kb on + strand, within BBR_RS13540 at 809.278 kb on + strand, within BBR_RS13540 at 809.291 kb on - strand, within BBR_RS13540 at 809.324 kb on + strand, within BBR_RS13540 at 809.324 kb on + strand, within BBR_RS13540 at 809.339 kb on - strand, within BBR_RS13540 at 809.359 kb on - strand, within BBR_RS13540 at 809.417 kb on + strand, within BBR_RS13540 at 809.417 kb on + strand, within BBR_RS13540 at 809.453 kb on + strand, within BBR_RS13540 at 809.453 kb on + strand, within BBR_RS13540 at 809.453 kb on + strand, within BBR_RS13540 at 809.453 kb on + strand, within BBR_RS13540 at 809.469 kb on + strand, within BBR_RS13540 at 809.537 kb on - strand, within BBR_RS13540 at 809.549 kb on + strand, within BBR_RS13540 at 809.555 kb on - strand, within BBR_RS13540 at 809.625 kb on + strand, within BBR_RS13540 at 809.636 kb on + strand, within BBR_RS13540 at 809.661 kb on - strand, within BBR_RS13540 at 809.661 kb on - strand, within BBR_RS13540 at 809.661 kb on - strand, within BBR_RS13540 at 809.665 kb on + strand, within BBR_RS13540 at 809.673 kb on - strand at 809.695 kb on + strand at 809.719 kb on - strand at 809.729 kb on - strand at 809.729 kb on - strand at 809.739 kb on + strand at 809.740 kb on + strand at 809.748 kb on - strand at 809.782 kb on - strand at 809.792 kb on - strand at 809.802 kb on - strand at 809.867 kb on + strand at 809.875 kb on - strand at 809.976 kb on - strand at 809.976 kb on - strand at 810.240 kb on - strand, within BBR_RS13545 at 810.458 kb on - strand, within BBR_RS13545 at 810.458 kb on - strand, within BBR_RS13545 at 810.552 kb on - strand, within BBR_RS13545 at 810.583 kb on + strand, within BBR_RS13545 at 810.586 kb on - strand, within BBR_RS13545 at 810.663 kb on + strand, within BBR_RS13545 at 810.681 kb on - strand, within BBR_RS13545 at 810.681 kb on - strand, within BBR_RS13545 at 810.747 kb on - strand, within BBR_RS13545
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 807,734 - BBR_RS13530 0.47 +0.1 807,787 + BBR_RS13530 0.57 +0.0 807,792 + BBR_RS13530 0.58 -0.1 807,797 + BBR_RS13530 0.59 -0.2 807,800 - BBR_RS13530 0.59 +0.3 807,800 - BBR_RS13530 0.59 +0.4 807,800 - BBR_RS13530 0.59 -0.0 807,800 - BBR_RS13530 0.59 +0.1 807,800 - BBR_RS13530 0.59 +0.3 807,800 - BBR_RS13530 0.59 -0.3 807,933 + BBR_RS13530 0.84 +0.5 807,955 - BBR_RS13530 0.88 -0.9 807,957 + BBR_RS13530 0.89 +2.1 807,998 + +0.1 808,000 - +0.9 808,277 + BBR_RS13535 0.11 +0.7 808,301 + BBR_RS13535 0.16 +0.1 808,304 - BBR_RS13535 0.17 -0.3 808,309 - BBR_RS13535 0.18 -0.5 808,309 - BBR_RS13535 0.18 -0.4 808,309 - BBR_RS13535 0.18 +0.0 808,354 - BBR_RS13535 0.28 -0.3 808,386 + BBR_RS13535 0.35 +0.2 808,392 - BBR_RS13535 0.36 -0.0 808,394 - BBR_RS13535 0.36 +0.1 808,394 - BBR_RS13535 0.36 +0.1 808,439 + BBR_RS13535 0.46 -0.4 808,447 - BBR_RS13535 0.48 +0.6 808,465 + BBR_RS13535 0.52 -0.0 808,495 - BBR_RS13535 0.58 +0.0 808,495 - BBR_RS13535 0.58 -0.4 808,509 + BBR_RS13535 0.61 -0.5 808,525 + BBR_RS13535 0.65 -0.5 808,554 + BBR_RS13535 0.71 -0.2 808,574 - BBR_RS13535 0.75 +0.1 808,574 - BBR_RS13535 0.75 -0.3 808,595 - BBR_RS13535 0.80 +0.6 808,656 + +0.1 808,664 - +0.4 808,664 - +0.5 808,664 - +0.1 808,803 + +0.8 808,811 - +1.2 808,821 - +0.1 808,822 - +0.0 808,823 + +0.9 808,831 - +1.2 808,838 + BBR_RS13540 0.10 -0.6 808,838 + BBR_RS13540 0.10 -0.3 808,846 - BBR_RS13540 0.11 -0.7 808,875 + BBR_RS13540 0.14 +0.3 808,883 - BBR_RS13540 0.15 +0.5 808,895 + BBR_RS13540 0.16 +0.5 808,895 + BBR_RS13540 0.16 -0.3 808,921 + BBR_RS13540 0.18 +0.5 808,924 + BBR_RS13540 0.19 +2.3 808,937 - BBR_RS13540 0.20 +0.6 809,088 + BBR_RS13540 0.34 +0.1 809,108 + BBR_RS13540 0.36 +0.3 809,120 - BBR_RS13540 0.37 +0.6 809,156 - BBR_RS13540 0.41 +0.5 809,184 - BBR_RS13540 0.43 +1.0 809,210 - BBR_RS13540 0.46 +0.7 809,227 + BBR_RS13540 0.48 +0.2 809,278 + BBR_RS13540 0.52 +0.7 809,291 - BBR_RS13540 0.54 -1.3 809,324 + BBR_RS13540 0.57 +0.4 809,324 + BBR_RS13540 0.57 +1.4 809,339 - BBR_RS13540 0.58 -0.0 809,359 - BBR_RS13540 0.60 +0.2 809,417 + BBR_RS13540 0.66 +0.1 809,417 + BBR_RS13540 0.66 +0.9 809,453 + BBR_RS13540 0.69 +0.4 809,453 + BBR_RS13540 0.69 +0.2 809,453 + BBR_RS13540 0.69 +1.7 809,453 + BBR_RS13540 0.69 -1.5 809,469 + BBR_RS13540 0.71 +0.2 809,537 - BBR_RS13540 0.77 +0.7 809,549 + BBR_RS13540 0.78 +0.3 809,555 - BBR_RS13540 0.79 +0.6 809,625 + BBR_RS13540 0.86 +1.1 809,636 + BBR_RS13540 0.87 +1.2 809,661 - BBR_RS13540 0.89 -1.1 809,661 - BBR_RS13540 0.89 -0.1 809,661 - BBR_RS13540 0.89 +0.3 809,665 + BBR_RS13540 0.90 +1.3 809,673 - +0.2 809,695 + -0.1 809,719 - -0.9 809,729 - -0.3 809,729 - -1.1 809,739 + +1.3 809,740 + -0.3 809,748 - +0.8 809,782 - -0.3 809,792 - +0.7 809,802 - -0.5 809,867 + +0.8 809,875 - +0.1 809,976 - -0.1 809,976 - +0.2 810,240 - BBR_RS13545 0.17 +0.3 810,458 - BBR_RS13545 0.33 +0.5 810,458 - BBR_RS13545 0.33 -0.5 810,552 - BBR_RS13545 0.40 -0.1 810,583 + BBR_RS13545 0.43 +0.4 810,586 - BBR_RS13545 0.43 -0.5 810,663 + BBR_RS13545 0.49 -0.6 810,681 - BBR_RS13545 0.50 +1.3 810,681 - BBR_RS13545 0.50 +0.0 810,747 - BBR_RS13545 0.55 -0.5
Or see this region's nucleotide sequence