Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13520 and BBR_RS13525 are separated by 80 nucleotides BBR_RS13525 and BBR_RS13530 overlap by 1 nucleotides BBR_RS13530 and BBR_RS13535 are separated by 207 nucleotides BBR_RS13535 and BBR_RS13540 are separated by 40 nucleotides
BBR_RS13520: BBR_RS13520 - polysaccharide synthesis protein GtrA, at 806,548 to 807,035
_RS13520
BBR_RS13525: BBR_RS13525 - CrcB family protein, at 807,116 to 807,481
_RS13525
BBR_RS13530: BBR_RS13530 - CrcB family protein, at 807,481 to 808,017
_RS13530
BBR_RS13535: BBR_RS13535 - MerR family DNA-binding transcriptional regulator, at 808,225 to 808,689
_RS13535
BBR_RS13540: BBR_RS13540 - alcohol dehydrogenase, at 808,730 to 809,773
_RS13540
Position (kb) 807
808
809 Strain fitness (log2 ratio) -1
0
1
2 at 806.495 kb on - strand at 806.497 kb on + strand at 806.530 kb on + strand at 806.538 kb on - strand at 806.539 kb on - strand at 806.564 kb on + strand at 806.606 kb on + strand, within BBR_RS13520 at 806.748 kb on + strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.767 kb on - strand, within BBR_RS13520 at 806.797 kb on + strand, within BBR_RS13520 at 806.821 kb on + strand, within BBR_RS13520 at 806.858 kb on + strand, within BBR_RS13520 at 806.866 kb on + strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.874 kb on - strand, within BBR_RS13520 at 806.875 kb on + strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.876 kb on - strand, within BBR_RS13520 at 806.878 kb on - strand, within BBR_RS13520 at 806.892 kb on + strand, within BBR_RS13520 at 806.902 kb on - strand, within BBR_RS13520 at 807.004 kb on - strand at 807.006 kb on - strand at 807.032 kb on - strand at 807.045 kb on - strand at 807.114 kb on + strand at 807.164 kb on - strand, within BBR_RS13525 at 807.164 kb on - strand, within BBR_RS13525 at 807.169 kb on + strand, within BBR_RS13525 at 807.186 kb on - strand, within BBR_RS13525 at 807.189 kb on + strand, within BBR_RS13525 at 807.201 kb on + strand, within BBR_RS13525 at 807.234 kb on + strand, within BBR_RS13525 at 807.253 kb on - strand, within BBR_RS13525 at 807.269 kb on + strand, within BBR_RS13525 at 807.409 kb on + strand, within BBR_RS13525 at 807.502 kb on - strand at 807.596 kb on + strand, within BBR_RS13530 at 807.603 kb on - strand, within BBR_RS13530 at 807.604 kb on - strand, within BBR_RS13530 at 807.638 kb on + strand, within BBR_RS13530 at 807.663 kb on + strand, within BBR_RS13530 at 807.691 kb on - strand, within BBR_RS13530 at 807.718 kb on - strand, within BBR_RS13530 at 807.734 kb on - strand, within BBR_RS13530 at 807.787 kb on + strand, within BBR_RS13530 at 807.792 kb on + strand, within BBR_RS13530 at 807.797 kb on + strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.800 kb on - strand, within BBR_RS13530 at 807.933 kb on + strand, within BBR_RS13530 at 807.955 kb on - strand, within BBR_RS13530 at 807.957 kb on + strand, within BBR_RS13530 at 807.998 kb on + strand at 808.000 kb on - strand at 808.277 kb on + strand, within BBR_RS13535 at 808.301 kb on + strand, within BBR_RS13535 at 808.304 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.309 kb on - strand, within BBR_RS13535 at 808.354 kb on - strand, within BBR_RS13535 at 808.386 kb on + strand, within BBR_RS13535 at 808.392 kb on - strand, within BBR_RS13535 at 808.394 kb on - strand, within BBR_RS13535 at 808.394 kb on - strand, within BBR_RS13535 at 808.439 kb on + strand, within BBR_RS13535 at 808.447 kb on - strand, within BBR_RS13535 at 808.465 kb on + strand, within BBR_RS13535 at 808.495 kb on - strand, within BBR_RS13535 at 808.495 kb on - strand, within BBR_RS13535 at 808.509 kb on + strand, within BBR_RS13535 at 808.525 kb on + strand, within BBR_RS13535 at 808.554 kb on + strand, within BBR_RS13535 at 808.574 kb on - strand, within BBR_RS13535 at 808.574 kb on - strand, within BBR_RS13535 at 808.595 kb on - strand, within BBR_RS13535 at 808.656 kb on + strand at 808.664 kb on - strand at 808.664 kb on - strand at 808.664 kb on - strand at 808.803 kb on + strand at 808.811 kb on - strand at 808.821 kb on - strand at 808.822 kb on - strand at 808.823 kb on + strand at 808.831 kb on - strand at 808.838 kb on + strand, within BBR_RS13540 at 808.838 kb on + strand, within BBR_RS13540 at 808.846 kb on - strand, within BBR_RS13540 at 808.875 kb on + strand, within BBR_RS13540 at 808.883 kb on - strand, within BBR_RS13540 at 808.895 kb on + strand, within BBR_RS13540 at 808.895 kb on + strand, within BBR_RS13540 at 808.921 kb on + strand, within BBR_RS13540 at 808.924 kb on + strand, within BBR_RS13540 at 808.937 kb on - strand, within BBR_RS13540
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 806,495 - -0.3 806,497 + +0.6 806,530 + -0.1 806,538 - +0.6 806,539 - +0.3 806,564 + -0.7 806,606 + BBR_RS13520 0.12 +0.1 806,748 + BBR_RS13520 0.41 -1.5 806,767 - BBR_RS13520 0.45 -0.2 806,767 - BBR_RS13520 0.45 -0.3 806,797 + BBR_RS13520 0.51 +0.7 806,821 + BBR_RS13520 0.56 +0.2 806,858 + BBR_RS13520 0.64 +0.2 806,866 + BBR_RS13520 0.65 +0.1 806,874 - BBR_RS13520 0.67 -0.4 806,874 - BBR_RS13520 0.67 +0.8 806,875 + BBR_RS13520 0.67 -0.5 806,876 - BBR_RS13520 0.67 +0.4 806,876 - BBR_RS13520 0.67 +0.7 806,878 - BBR_RS13520 0.68 +0.0 806,892 + BBR_RS13520 0.70 +0.2 806,902 - BBR_RS13520 0.73 -1.3 807,004 - +0.6 807,006 - -0.7 807,032 - -0.3 807,045 - +0.7 807,114 + -0.3 807,164 - BBR_RS13525 0.13 -0.7 807,164 - BBR_RS13525 0.13 +0.5 807,169 + BBR_RS13525 0.14 +0.3 807,186 - BBR_RS13525 0.19 -0.0 807,189 + BBR_RS13525 0.20 -0.4 807,201 + BBR_RS13525 0.23 +0.2 807,234 + BBR_RS13525 0.32 -0.1 807,253 - BBR_RS13525 0.37 +0.0 807,269 + BBR_RS13525 0.42 +0.8 807,409 + BBR_RS13525 0.80 -0.2 807,502 - +0.2 807,596 + BBR_RS13530 0.21 -0.2 807,603 - BBR_RS13530 0.23 -0.1 807,604 - BBR_RS13530 0.23 +0.5 807,638 + BBR_RS13530 0.29 -0.2 807,663 + BBR_RS13530 0.34 +1.3 807,691 - BBR_RS13530 0.39 +1.0 807,718 - BBR_RS13530 0.44 +0.7 807,734 - BBR_RS13530 0.47 +0.1 807,787 + BBR_RS13530 0.57 +0.0 807,792 + BBR_RS13530 0.58 -0.1 807,797 + BBR_RS13530 0.59 -0.2 807,800 - BBR_RS13530 0.59 +0.3 807,800 - BBR_RS13530 0.59 +0.4 807,800 - BBR_RS13530 0.59 -0.0 807,800 - BBR_RS13530 0.59 +0.1 807,800 - BBR_RS13530 0.59 +0.3 807,800 - BBR_RS13530 0.59 -0.3 807,933 + BBR_RS13530 0.84 +0.5 807,955 - BBR_RS13530 0.88 -0.9 807,957 + BBR_RS13530 0.89 +2.1 807,998 + +0.1 808,000 - +0.9 808,277 + BBR_RS13535 0.11 +0.7 808,301 + BBR_RS13535 0.16 +0.1 808,304 - BBR_RS13535 0.17 -0.3 808,309 - BBR_RS13535 0.18 -0.5 808,309 - BBR_RS13535 0.18 -0.4 808,309 - BBR_RS13535 0.18 +0.0 808,354 - BBR_RS13535 0.28 -0.3 808,386 + BBR_RS13535 0.35 +0.2 808,392 - BBR_RS13535 0.36 -0.0 808,394 - BBR_RS13535 0.36 +0.1 808,394 - BBR_RS13535 0.36 +0.1 808,439 + BBR_RS13535 0.46 -0.4 808,447 - BBR_RS13535 0.48 +0.6 808,465 + BBR_RS13535 0.52 -0.0 808,495 - BBR_RS13535 0.58 +0.0 808,495 - BBR_RS13535 0.58 -0.4 808,509 + BBR_RS13535 0.61 -0.5 808,525 + BBR_RS13535 0.65 -0.5 808,554 + BBR_RS13535 0.71 -0.2 808,574 - BBR_RS13535 0.75 +0.1 808,574 - BBR_RS13535 0.75 -0.3 808,595 - BBR_RS13535 0.80 +0.6 808,656 + +0.1 808,664 - +0.4 808,664 - +0.5 808,664 - +0.1 808,803 + +0.8 808,811 - +1.2 808,821 - +0.1 808,822 - +0.0 808,823 + +0.9 808,831 - +1.2 808,838 + BBR_RS13540 0.10 -0.6 808,838 + BBR_RS13540 0.10 -0.3 808,846 - BBR_RS13540 0.11 -0.7 808,875 + BBR_RS13540 0.14 +0.3 808,883 - BBR_RS13540 0.15 +0.5 808,895 + BBR_RS13540 0.16 +0.5 808,895 + BBR_RS13540 0.16 -0.3 808,921 + BBR_RS13540 0.18 +0.5 808,924 + BBR_RS13540 0.19 +2.3 808,937 - BBR_RS13540 0.20 +0.6
Or see this region's nucleotide sequence