Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13010 and BBR_RS13015 are separated by 143 nucleotides BBR_RS13015 and BBR_RS13020 overlap by 4 nucleotides
BBR_RS13010: BBR_RS13010 - phosphoglucosamine mutase, at 691,532 to 692,917
_RS13010
BBR_RS13015: BBR_RS13015 - peptide deformylase, at 693,061 to 693,714
_RS13015
BBR_RS13020: BBR_RS13020 - DNA polymerase III subunit epsilon, at 693,711 to 695,165
_RS13020
Position (kb)
693
694 Strain fitness (log2 ratio)
-2
-1
0
1 at 692.920 kb on - strand at 692.920 kb on - strand at 692.935 kb on - strand at 692.935 kb on - strand at 692.941 kb on + strand at 692.948 kb on + strand at 692.949 kb on - strand at 692.961 kb on + strand at 692.969 kb on + strand at 692.971 kb on + strand at 692.983 kb on + strand at 692.984 kb on + strand at 692.998 kb on + strand at 693.028 kb on + strand at 693.144 kb on - strand, within BBR_RS13015 at 693.144 kb on - strand, within BBR_RS13015 at 693.252 kb on - strand, within BBR_RS13015 at 693.257 kb on + strand, within BBR_RS13015 at 693.280 kb on - strand, within BBR_RS13015 at 693.284 kb on - strand, within BBR_RS13015 at 693.322 kb on + strand, within BBR_RS13015 at 693.330 kb on - strand, within BBR_RS13015 at 693.330 kb on - strand, within BBR_RS13015 at 693.351 kb on - strand, within BBR_RS13015 at 693.395 kb on + strand, within BBR_RS13015 at 693.402 kb on + strand, within BBR_RS13015 at 693.452 kb on + strand, within BBR_RS13015 at 693.462 kb on + strand, within BBR_RS13015 at 693.469 kb on - strand, within BBR_RS13015 at 693.470 kb on - strand, within BBR_RS13015 at 693.470 kb on - strand, within BBR_RS13015 at 693.511 kb on + strand, within BBR_RS13015 at 693.534 kb on - strand, within BBR_RS13015 at 693.551 kb on + strand, within BBR_RS13015 at 693.556 kb on + strand, within BBR_RS13015 at 693.570 kb on + strand, within BBR_RS13015 at 693.570 kb on + strand, within BBR_RS13015 at 693.578 kb on + strand, within BBR_RS13015 at 693.578 kb on + strand, within BBR_RS13015 at 693.583 kb on - strand, within BBR_RS13015 at 693.662 kb on - strand at 693.662 kb on - strand at 693.727 kb on + strand at 693.739 kb on + strand at 693.744 kb on + strand at 693.775 kb on + strand at 693.793 kb on + strand at 693.819 kb on - strand at 693.819 kb on - strand at 693.820 kb on + strand at 693.820 kb on + strand at 693.828 kb on - strand at 693.828 kb on - strand at 693.846 kb on - strand at 693.852 kb on + strand at 693.899 kb on + strand, within BBR_RS13020 at 693.907 kb on - strand, within BBR_RS13020 at 693.927 kb on - strand, within BBR_RS13020 at 693.949 kb on - strand, within BBR_RS13020 at 694.007 kb on + strand, within BBR_RS13020 at 694.007 kb on + strand, within BBR_RS13020 at 694.015 kb on - strand, within BBR_RS13020 at 694.015 kb on - strand, within BBR_RS13020 at 694.029 kb on + strand, within BBR_RS13020 at 694.037 kb on + strand, within BBR_RS13020 at 694.037 kb on + strand, within BBR_RS13020 at 694.087 kb on + strand, within BBR_RS13020 at 694.095 kb on - strand, within BBR_RS13020 at 694.096 kb on + strand, within BBR_RS13020 at 694.129 kb on - strand, within BBR_RS13020 at 694.155 kb on + strand, within BBR_RS13020 at 694.167 kb on + strand, within BBR_RS13020 at 694.167 kb on + strand, within BBR_RS13020 at 694.186 kb on - strand, within BBR_RS13020 at 694.186 kb on - strand, within BBR_RS13020 at 694.207 kb on + strand, within BBR_RS13020 at 694.207 kb on + strand, within BBR_RS13020 at 694.209 kb on + strand, within BBR_RS13020 at 694.230 kb on + strand, within BBR_RS13020 at 694.230 kb on + strand, within BBR_RS13020 at 694.230 kb on + strand, within BBR_RS13020 at 694.230 kb on + strand, within BBR_RS13020 at 694.230 kb on + strand, within BBR_RS13020 at 694.235 kb on + strand, within BBR_RS13020 at 694.238 kb on - strand, within BBR_RS13020 at 694.238 kb on - strand, within BBR_RS13020 at 694.238 kb on - strand, within BBR_RS13020 at 694.274 kb on + strand, within BBR_RS13020 at 694.274 kb on + strand, within BBR_RS13020 at 694.274 kb on + strand, within BBR_RS13020 at 694.285 kb on + strand, within BBR_RS13020 at 694.288 kb on - strand, within BBR_RS13020 at 694.305 kb on + strand, within BBR_RS13020 at 694.309 kb on + strand, within BBR_RS13020 at 694.324 kb on + strand, within BBR_RS13020 at 694.339 kb on - strand, within BBR_RS13020 at 694.339 kb on - strand, within BBR_RS13020 at 694.350 kb on + strand, within BBR_RS13020 at 694.352 kb on + strand, within BBR_RS13020 at 694.375 kb on + strand, within BBR_RS13020 at 694.390 kb on + strand, within BBR_RS13020 at 694.455 kb on - strand, within BBR_RS13020 at 694.470 kb on - strand, within BBR_RS13020 at 694.577 kb on - strand, within BBR_RS13020 at 694.600 kb on + strand, within BBR_RS13020 at 694.603 kb on - strand, within BBR_RS13020 at 694.618 kb on + strand, within BBR_RS13020 at 694.626 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020 at 694.653 kb on - strand, within BBR_RS13020
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 692,920 - +0.4 692,920 - +0.3 692,935 - +0.4 692,935 - +0.3 692,941 + -0.8 692,948 + +0.1 692,949 - +0.6 692,961 + -2.2 692,969 + +0.6 692,971 + +0.4 692,983 + +0.3 692,984 + -1.1 692,998 + +0.6 693,028 + +0.2 693,144 - BBR_RS13015 0.13 +0.6 693,144 - BBR_RS13015 0.13 -0.7 693,252 - BBR_RS13015 0.29 -0.1 693,257 + BBR_RS13015 0.30 +0.4 693,280 - BBR_RS13015 0.33 +0.7 693,284 - BBR_RS13015 0.34 +0.2 693,322 + BBR_RS13015 0.40 +0.3 693,330 - BBR_RS13015 0.41 +0.5 693,330 - BBR_RS13015 0.41 +0.9 693,351 - BBR_RS13015 0.44 +1.3 693,395 + BBR_RS13015 0.51 +0.1 693,402 + BBR_RS13015 0.52 +0.0 693,452 + BBR_RS13015 0.60 -1.1 693,462 + BBR_RS13015 0.61 -0.6 693,469 - BBR_RS13015 0.62 +0.1 693,470 - BBR_RS13015 0.63 +0.4 693,470 - BBR_RS13015 0.63 +0.2 693,511 + BBR_RS13015 0.69 -0.3 693,534 - BBR_RS13015 0.72 +0.6 693,551 + BBR_RS13015 0.75 +0.1 693,556 + BBR_RS13015 0.76 -1.0 693,570 + BBR_RS13015 0.78 -0.1 693,570 + BBR_RS13015 0.78 +0.9 693,578 + BBR_RS13015 0.79 +0.9 693,578 + BBR_RS13015 0.79 -0.5 693,583 - BBR_RS13015 0.80 -0.1 693,662 - -0.4 693,662 - +0.5 693,727 + +1.0 693,739 + -0.5 693,744 + -0.2 693,775 + -0.1 693,793 + +0.4 693,819 - +0.8 693,819 - +0.0 693,820 + -0.1 693,820 + +0.6 693,828 - +0.4 693,828 - +0.5 693,846 - +0.7 693,852 + +0.2 693,899 + BBR_RS13020 0.13 -0.7 693,907 - BBR_RS13020 0.13 -0.7 693,927 - BBR_RS13020 0.15 -0.3 693,949 - BBR_RS13020 0.16 -0.7 694,007 + BBR_RS13020 0.20 +0.2 694,007 + BBR_RS13020 0.20 +0.5 694,015 - BBR_RS13020 0.21 -0.1 694,015 - BBR_RS13020 0.21 -0.7 694,029 + BBR_RS13020 0.22 +0.5 694,037 + BBR_RS13020 0.22 -0.0 694,037 + BBR_RS13020 0.22 +0.1 694,087 + BBR_RS13020 0.26 -1.2 694,095 - BBR_RS13020 0.26 -0.3 694,096 + BBR_RS13020 0.26 +0.9 694,129 - BBR_RS13020 0.29 -0.1 694,155 + BBR_RS13020 0.31 +0.3 694,167 + BBR_RS13020 0.31 +0.2 694,167 + BBR_RS13020 0.31 +0.1 694,186 - BBR_RS13020 0.33 +0.3 694,186 - BBR_RS13020 0.33 +0.4 694,207 + BBR_RS13020 0.34 -0.2 694,207 + BBR_RS13020 0.34 -0.0 694,209 + BBR_RS13020 0.34 +0.6 694,230 + BBR_RS13020 0.36 +0.3 694,230 + BBR_RS13020 0.36 -0.1 694,230 + BBR_RS13020 0.36 -0.4 694,230 + BBR_RS13020 0.36 +0.6 694,230 + BBR_RS13020 0.36 -0.2 694,235 + BBR_RS13020 0.36 +0.1 694,238 - BBR_RS13020 0.36 -1.3 694,238 - BBR_RS13020 0.36 -0.7 694,238 - BBR_RS13020 0.36 -0.4 694,274 + BBR_RS13020 0.39 -0.2 694,274 + BBR_RS13020 0.39 +0.9 694,274 + BBR_RS13020 0.39 -0.2 694,285 + BBR_RS13020 0.39 -0.4 694,288 - BBR_RS13020 0.40 -0.2 694,305 + BBR_RS13020 0.41 +0.5 694,309 + BBR_RS13020 0.41 +0.6 694,324 + BBR_RS13020 0.42 -0.2 694,339 - BBR_RS13020 0.43 +0.9 694,339 - BBR_RS13020 0.43 +0.4 694,350 + BBR_RS13020 0.44 -0.2 694,352 + BBR_RS13020 0.44 +0.2 694,375 + BBR_RS13020 0.46 +0.6 694,390 + BBR_RS13020 0.47 +0.6 694,455 - BBR_RS13020 0.51 +0.8 694,470 - BBR_RS13020 0.52 +0.2 694,577 - BBR_RS13020 0.60 +0.4 694,600 + BBR_RS13020 0.61 +0.2 694,603 - BBR_RS13020 0.61 -0.0 694,618 + BBR_RS13020 0.62 +0.5 694,626 - BBR_RS13020 0.63 +0.2 694,653 - BBR_RS13020 0.65 -0.3 694,653 - BBR_RS13020 0.65 +0.8 694,653 - BBR_RS13020 0.65 -0.3
Or see this region's nucleotide sequence