Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12910 and BBR_RS20735 are separated by 411 nucleotides BBR_RS20735 and BBR_RS12915 are separated by 72 nucleotides BBR_RS12915 and BBR_RS12920 are separated by 141 nucleotides
BBR_RS12910: BBR_RS12910 - galactokinase, at 648,466 to 649,716
_RS12910
BBR_RS20735: BBR_RS20735 - hypothetical protein, at 650,128 to 650,412
_RS20735
BBR_RS12915: BBR_RS12915 - glycine synthesis protein GlyXS <small>(from data)</small>, at 650,485 to 650,757
_RS12915
BBR_RS12920: BBR_RS12920 - glycine synthesis protein GlyXL <small>(from data)</small>, at 650,899 to 652,263
_RS12920
Position (kb) 650
651 Strain fitness (log2 ratio) -1
0
1
2 at 649.496 kb on + strand, within BBR_RS12910 at 649.506 kb on + strand, within BBR_RS12910 at 649.522 kb on - strand, within BBR_RS12910 at 649.522 kb on - strand, within BBR_RS12910 at 649.533 kb on + strand, within BBR_RS12910 at 649.539 kb on + strand, within BBR_RS12910 at 649.559 kb on - strand, within BBR_RS12910 at 649.575 kb on - strand, within BBR_RS12910 at 649.594 kb on - strand at 649.597 kb on + strand at 649.602 kb on + strand at 649.605 kb on - strand at 649.607 kb on + strand at 649.631 kb on + strand at 649.640 kb on + strand at 649.666 kb on + strand at 649.685 kb on + strand at 649.718 kb on - strand at 649.747 kb on - strand at 649.753 kb on + strand at 649.757 kb on - strand at 649.778 kb on + strand at 649.778 kb on + strand at 649.786 kb on - strand at 649.786 kb on - strand at 649.788 kb on - strand at 649.860 kb on + strand at 649.862 kb on + strand at 649.868 kb on - strand at 649.872 kb on + strand at 649.882 kb on + strand at 649.909 kb on + strand at 649.917 kb on - strand at 650.020 kb on - strand at 650.034 kb on + strand at 650.072 kb on + strand at 650.072 kb on + strand at 650.073 kb on - strand at 650.085 kb on - strand at 650.172 kb on + strand, within BBR_RS20735 at 650.199 kb on - strand, within BBR_RS20735 at 650.199 kb on - strand, within BBR_RS20735 at 650.224 kb on + strand, within BBR_RS20735 at 650.227 kb on + strand, within BBR_RS20735 at 650.232 kb on + strand, within BBR_RS20735 at 650.258 kb on + strand, within BBR_RS20735 at 650.259 kb on - strand, within BBR_RS20735 at 650.275 kb on + strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.347 kb on - strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.349 kb on + strand, within BBR_RS20735 at 650.357 kb on - strand, within BBR_RS20735 at 650.357 kb on - strand, within BBR_RS20735 at 650.363 kb on + strand, within BBR_RS20735 at 650.374 kb on + strand, within BBR_RS20735 at 650.388 kb on + strand at 650.389 kb on - strand at 650.433 kb on + strand at 650.435 kb on + strand at 650.443 kb on - strand at 650.443 kb on - strand at 650.451 kb on - strand at 650.451 kb on - strand at 650.476 kb on + strand at 650.478 kb on - strand at 650.480 kb on - strand at 650.493 kb on + strand at 650.494 kb on + strand at 650.512 kb on + strand at 650.541 kb on + strand, within BBR_RS12915 at 650.541 kb on + strand, within BBR_RS12915 at 650.546 kb on + strand, within BBR_RS12915 at 650.546 kb on + strand, within BBR_RS12915 at 650.549 kb on - strand, within BBR_RS12915 at 650.567 kb on + strand, within BBR_RS12915 at 650.567 kb on + strand, within BBR_RS12915 at 650.660 kb on + strand, within BBR_RS12915 at 650.744 kb on - strand at 650.768 kb on + strand at 650.804 kb on - strand at 650.808 kb on + strand at 650.808 kb on + strand at 650.868 kb on - strand at 650.898 kb on - strand at 650.937 kb on - strand at 650.970 kb on + strand at 650.986 kb on + strand at 651.006 kb on - strand at 651.009 kb on + strand at 651.038 kb on - strand, within BBR_RS12920 at 651.054 kb on + strand, within BBR_RS12920 at 651.069 kb on - strand, within BBR_RS12920 at 651.076 kb on + strand, within BBR_RS12920 at 651.076 kb on + strand, within BBR_RS12920 at 651.084 kb on - strand, within BBR_RS12920 at 651.084 kb on - strand, within BBR_RS12920 at 651.102 kb on - strand, within BBR_RS12920 at 651.120 kb on + strand, within BBR_RS12920 at 651.130 kb on - strand, within BBR_RS12920 at 651.204 kb on + strand, within BBR_RS12920 at 651.212 kb on - strand, within BBR_RS12920 at 651.235 kb on - strand, within BBR_RS12920 at 651.268 kb on + strand, within BBR_RS12920 at 651.294 kb on + strand, within BBR_RS12920 at 651.294 kb on + strand, within BBR_RS12920 at 651.294 kb on + strand, within BBR_RS12920 at 651.314 kb on - strand, within BBR_RS12920 at 651.360 kb on + strand, within BBR_RS12920 at 651.360 kb on + strand, within BBR_RS12920 at 651.361 kb on + strand, within BBR_RS12920 at 651.386 kb on - strand, within BBR_RS12920 at 651.409 kb on + strand, within BBR_RS12920 at 651.436 kb on + strand, within BBR_RS12920 at 651.482 kb on + strand, within BBR_RS12920 at 651.484 kb on + strand, within BBR_RS12920 at 651.504 kb on + strand, within BBR_RS12920 at 651.511 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.529 kb on - strand, within BBR_RS12920 at 651.594 kb on + strand, within BBR_RS12920 at 651.594 kb on + strand, within BBR_RS12920 at 651.601 kb on + strand, within BBR_RS12920 at 651.601 kb on + strand, within BBR_RS12920 at 651.634 kb on - strand, within BBR_RS12920 at 651.645 kb on + strand, within BBR_RS12920 at 651.669 kb on + strand, within BBR_RS12920 at 651.669 kb on + strand, within BBR_RS12920 at 651.742 kb on - strand, within BBR_RS12920
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 649,496 + BBR_RS12910 0.82 +0.7 649,506 + BBR_RS12910 0.83 +0.3 649,522 - BBR_RS12910 0.84 +0.6 649,522 - BBR_RS12910 0.84 +0.4 649,533 + BBR_RS12910 0.85 +0.2 649,539 + BBR_RS12910 0.86 -0.3 649,559 - BBR_RS12910 0.87 -0.2 649,575 - BBR_RS12910 0.89 +0.7 649,594 - +0.1 649,597 + +0.4 649,602 + +1.6 649,605 - +0.5 649,607 + +0.5 649,631 + +0.2 649,640 + +0.7 649,666 + +0.1 649,685 + +0.6 649,718 - -0.1 649,747 - +0.6 649,753 + -0.4 649,757 - -0.1 649,778 + -0.0 649,778 + -0.2 649,786 - -0.7 649,786 - -0.0 649,788 - -0.3 649,860 + +0.0 649,862 + -0.2 649,868 - -0.6 649,872 + -0.0 649,882 + +0.9 649,909 + +0.5 649,917 - -0.5 650,020 - -1.4 650,034 + +0.0 650,072 + +0.4 650,072 + -0.9 650,073 - +1.1 650,085 - -0.5 650,172 + BBR_RS20735 0.15 -0.1 650,199 - BBR_RS20735 0.25 -0.3 650,199 - BBR_RS20735 0.25 +0.1 650,224 + BBR_RS20735 0.34 +0.2 650,227 + BBR_RS20735 0.35 +0.1 650,232 + BBR_RS20735 0.36 +0.1 650,258 + BBR_RS20735 0.46 +0.4 650,259 - BBR_RS20735 0.46 +1.4 650,275 + BBR_RS20735 0.52 +0.6 650,347 - BBR_RS20735 0.77 -0.0 650,347 - BBR_RS20735 0.77 +2.4 650,347 - BBR_RS20735 0.77 +0.1 650,347 - BBR_RS20735 0.77 -0.2 650,349 + BBR_RS20735 0.78 -0.4 650,349 + BBR_RS20735 0.78 -1.5 650,349 + BBR_RS20735 0.78 +0.8 650,349 + BBR_RS20735 0.78 +0.2 650,349 + BBR_RS20735 0.78 -0.7 650,357 - BBR_RS20735 0.80 -0.1 650,357 - BBR_RS20735 0.80 -0.8 650,363 + BBR_RS20735 0.82 -0.1 650,374 + BBR_RS20735 0.86 -0.2 650,388 + +0.4 650,389 - -0.3 650,433 + +0.5 650,435 + +0.2 650,443 - +0.1 650,443 - +0.8 650,451 - +0.6 650,451 - +0.5 650,476 + +0.3 650,478 - +0.8 650,480 - -0.0 650,493 + +1.0 650,494 + -1.1 650,512 + -0.0 650,541 + BBR_RS12915 0.21 +0.4 650,541 + BBR_RS12915 0.21 +0.0 650,546 + BBR_RS12915 0.22 +0.2 650,546 + BBR_RS12915 0.22 -0.1 650,549 - BBR_RS12915 0.23 +0.9 650,567 + BBR_RS12915 0.30 -0.5 650,567 + BBR_RS12915 0.30 +1.0 650,660 + BBR_RS12915 0.64 -0.1 650,744 - +0.5 650,768 + -0.2 650,804 - -0.3 650,808 + +0.0 650,808 + -0.1 650,868 - -0.6 650,898 - +0.1 650,937 - -0.6 650,970 + -0.6 650,986 + +2.1 651,006 - +0.2 651,009 + +0.0 651,038 - BBR_RS12920 0.10 +0.2 651,054 + BBR_RS12920 0.11 -0.1 651,069 - BBR_RS12920 0.12 -0.8 651,076 + BBR_RS12920 0.13 +0.1 651,076 + BBR_RS12920 0.13 +0.1 651,084 - BBR_RS12920 0.14 +0.8 651,084 - BBR_RS12920 0.14 -0.4 651,102 - BBR_RS12920 0.15 -0.3 651,120 + BBR_RS12920 0.16 +0.1 651,130 - BBR_RS12920 0.17 +0.2 651,204 + BBR_RS12920 0.22 +0.2 651,212 - BBR_RS12920 0.23 -0.2 651,235 - BBR_RS12920 0.25 -0.2 651,268 + BBR_RS12920 0.27 +0.1 651,294 + BBR_RS12920 0.29 -0.0 651,294 + BBR_RS12920 0.29 -0.3 651,294 + BBR_RS12920 0.29 -0.6 651,314 - BBR_RS12920 0.30 -0.5 651,360 + BBR_RS12920 0.34 +0.7 651,360 + BBR_RS12920 0.34 -0.7 651,361 + BBR_RS12920 0.34 +0.0 651,386 - BBR_RS12920 0.36 -0.5 651,409 + BBR_RS12920 0.37 -0.3 651,436 + BBR_RS12920 0.39 +0.0 651,482 + BBR_RS12920 0.43 -0.1 651,484 + BBR_RS12920 0.43 +0.7 651,504 + BBR_RS12920 0.44 +1.4 651,511 + BBR_RS12920 0.45 -0.1 651,526 + BBR_RS12920 0.46 -0.2 651,526 + BBR_RS12920 0.46 +0.8 651,526 + BBR_RS12920 0.46 +0.8 651,526 + BBR_RS12920 0.46 +0.3 651,529 - BBR_RS12920 0.46 +0.4 651,594 + BBR_RS12920 0.51 +0.3 651,594 + BBR_RS12920 0.51 -0.6 651,601 + BBR_RS12920 0.51 +0.1 651,601 + BBR_RS12920 0.51 +0.5 651,634 - BBR_RS12920 0.54 -0.6 651,645 + BBR_RS12920 0.55 -0.3 651,669 + BBR_RS12920 0.56 +0.0 651,669 + BBR_RS12920 0.56 -0.9 651,742 - BBR_RS12920 0.62 +0.6
Or see this region's nucleotide sequence