Experiment: Recovery broth with Isovaleric 75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12760 and BBR_RS12770 are separated by 70 nucleotides BBR_RS12770 and BBR_RS12775 are separated by 469 nucleotides BBR_RS12775 and BBR_RS12780 are separated by 0 nucleotides
BBR_RS12760: BBR_RS12760 - hypothetical protein, at 615,670 to 616,906
_RS12760
BBR_RS12770: BBR_RS12770 - hypothetical protein, at 616,977 to 617,183
_RS12770
BBR_RS12775: BBR_RS12775 - hypothetical protein, at 617,653 to 618,066
_RS12775
BBR_RS12780: BBR_RS12780 - hypothetical protein, at 618,067 to 618,603
_RS12780
Position (kb)
616
617
618 Strain fitness (log2 ratio)
-1
0
1
2 at 615.988 kb on - strand, within BBR_RS12760 at 616.086 kb on - strand, within BBR_RS12760 at 616.087 kb on - strand, within BBR_RS12760 at 616.103 kb on - strand, within BBR_RS12760 at 616.140 kb on + strand, within BBR_RS12760 at 616.140 kb on + strand, within BBR_RS12760 at 616.146 kb on + strand, within BBR_RS12760 at 616.148 kb on - strand, within BBR_RS12760 at 616.148 kb on - strand, within BBR_RS12760 at 616.153 kb on + strand, within BBR_RS12760 at 616.154 kb on - strand, within BBR_RS12760 at 616.154 kb on - strand, within BBR_RS12760 at 616.169 kb on - strand, within BBR_RS12760 at 616.178 kb on + strand, within BBR_RS12760 at 616.187 kb on - strand, within BBR_RS12760 at 616.201 kb on + strand, within BBR_RS12760 at 616.201 kb on + strand, within BBR_RS12760 at 616.202 kb on - strand, within BBR_RS12760 at 616.209 kb on - strand, within BBR_RS12760 at 616.273 kb on + strand, within BBR_RS12760 at 616.304 kb on - strand, within BBR_RS12760 at 616.305 kb on + strand, within BBR_RS12760 at 616.307 kb on + strand, within BBR_RS12760 at 616.338 kb on + strand, within BBR_RS12760 at 616.338 kb on + strand, within BBR_RS12760 at 616.345 kb on - strand, within BBR_RS12760 at 616.379 kb on + strand, within BBR_RS12760 at 616.379 kb on + strand, within BBR_RS12760 at 616.450 kb on + strand, within BBR_RS12760 at 616.536 kb on - strand, within BBR_RS12760 at 616.689 kb on + strand, within BBR_RS12760 at 616.706 kb on - strand, within BBR_RS12760 at 616.721 kb on - strand, within BBR_RS12760 at 616.779 kb on - strand, within BBR_RS12760 at 616.799 kb on + strand at 616.799 kb on + strand at 616.799 kb on + strand at 616.807 kb on - strand at 616.807 kb on - strand at 616.814 kb on - strand at 616.814 kb on - strand at 616.825 kb on - strand at 616.845 kb on - strand at 616.871 kb on + strand at 616.879 kb on - strand at 616.887 kb on + strand at 616.891 kb on - strand at 616.899 kb on - strand at 617.039 kb on + strand, within BBR_RS12770 at 617.053 kb on + strand, within BBR_RS12770 at 617.067 kb on + strand, within BBR_RS12770 at 617.085 kb on + strand, within BBR_RS12770 at 617.093 kb on - strand, within BBR_RS12770 at 617.103 kb on - strand, within BBR_RS12770 at 617.143 kb on - strand, within BBR_RS12770 at 617.177 kb on + strand at 617.215 kb on - strand at 617.215 kb on - strand at 617.221 kb on - strand at 617.261 kb on + strand at 617.263 kb on + strand at 617.294 kb on - strand at 617.343 kb on + strand at 617.345 kb on + strand at 617.345 kb on + strand at 617.345 kb on + strand at 617.351 kb on - strand at 617.353 kb on + strand at 617.353 kb on + strand at 617.415 kb on + strand at 617.439 kb on + strand at 617.454 kb on - strand at 617.462 kb on - strand at 617.529 kb on + strand at 617.555 kb on - strand at 617.560 kb on + strand at 617.601 kb on - strand at 617.627 kb on + strand at 617.627 kb on + strand at 617.627 kb on + strand at 617.627 kb on + strand at 617.627 kb on + strand at 617.672 kb on + strand at 617.680 kb on - strand at 617.735 kb on + strand, within BBR_RS12775 at 617.803 kb on - strand, within BBR_RS12775 at 617.863 kb on + strand, within BBR_RS12775 at 617.865 kb on - strand, within BBR_RS12775 at 617.928 kb on + strand, within BBR_RS12775 at 617.928 kb on + strand, within BBR_RS12775 at 617.928 kb on + strand, within BBR_RS12775 at 617.928 kb on + strand, within BBR_RS12775 at 617.928 kb on + strand, within BBR_RS12775 at 617.936 kb on + strand, within BBR_RS12775 at 617.936 kb on - strand, within BBR_RS12775 at 617.936 kb on - strand, within BBR_RS12775 at 617.936 kb on - strand, within BBR_RS12775 at 618.019 kb on + strand, within BBR_RS12775 at 618.021 kb on + strand, within BBR_RS12775 at 618.038 kb on + strand at 618.055 kb on + strand at 618.110 kb on - strand at 618.115 kb on - strand at 618.157 kb on - strand, within BBR_RS12780 at 618.169 kb on - strand, within BBR_RS12780
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Isovaleric 75 mM remove 615,988 - BBR_RS12760 0.26 -0.2 616,086 - BBR_RS12760 0.34 +0.8 616,087 - BBR_RS12760 0.34 -0.6 616,103 - BBR_RS12760 0.35 -0.7 616,140 + BBR_RS12760 0.38 +0.9 616,140 + BBR_RS12760 0.38 -0.1 616,146 + BBR_RS12760 0.38 +0.1 616,148 - BBR_RS12760 0.39 +0.2 616,148 - BBR_RS12760 0.39 +0.3 616,153 + BBR_RS12760 0.39 +0.2 616,154 - BBR_RS12760 0.39 +1.4 616,154 - BBR_RS12760 0.39 -0.4 616,169 - BBR_RS12760 0.40 -0.3 616,178 + BBR_RS12760 0.41 +0.6 616,187 - BBR_RS12760 0.42 -1.0 616,201 + BBR_RS12760 0.43 -0.5 616,201 + BBR_RS12760 0.43 +0.2 616,202 - BBR_RS12760 0.43 +0.2 616,209 - BBR_RS12760 0.44 -0.1 616,273 + BBR_RS12760 0.49 +0.8 616,304 - BBR_RS12760 0.51 -0.5 616,305 + BBR_RS12760 0.51 +1.0 616,307 + BBR_RS12760 0.51 +0.2 616,338 + BBR_RS12760 0.54 +0.3 616,338 + BBR_RS12760 0.54 -0.3 616,345 - BBR_RS12760 0.55 +0.6 616,379 + BBR_RS12760 0.57 -0.8 616,379 + BBR_RS12760 0.57 +0.5 616,450 + BBR_RS12760 0.63 +0.8 616,536 - BBR_RS12760 0.70 -0.1 616,689 + BBR_RS12760 0.82 +1.5 616,706 - BBR_RS12760 0.84 +0.7 616,721 - BBR_RS12760 0.85 -0.3 616,779 - BBR_RS12760 0.90 +0.0 616,799 + +1.9 616,799 + +2.4 616,799 + -0.6 616,807 - +0.0 616,807 - +0.5 616,814 - +0.7 616,814 - +0.5 616,825 - +0.2 616,845 - +0.5 616,871 + -0.4 616,879 - +0.7 616,887 + +1.3 616,891 - -0.7 616,899 - -0.1 617,039 + BBR_RS12770 0.30 -0.5 617,053 + BBR_RS12770 0.37 +0.6 617,067 + BBR_RS12770 0.43 +0.7 617,085 + BBR_RS12770 0.52 -1.3 617,093 - BBR_RS12770 0.56 +0.2 617,103 - BBR_RS12770 0.61 +0.2 617,143 - BBR_RS12770 0.80 +0.5 617,177 + -0.2 617,215 - -0.5 617,215 - +0.1 617,221 - +0.3 617,261 + -0.3 617,263 + +0.0 617,294 - +0.8 617,343 + +0.3 617,345 + +0.0 617,345 + +0.7 617,345 + -0.7 617,351 - -0.3 617,353 + +1.3 617,353 + +0.1 617,415 + +0.6 617,439 + -0.0 617,454 - -0.4 617,462 - +1.1 617,529 + +0.0 617,555 - +0.3 617,560 + -0.7 617,601 - +0.3 617,627 + -0.5 617,627 + +0.2 617,627 + -1.7 617,627 + -0.3 617,627 + +0.2 617,672 + +0.6 617,680 - -0.9 617,735 + BBR_RS12775 0.20 +0.3 617,803 - BBR_RS12775 0.36 +0.1 617,863 + BBR_RS12775 0.51 +0.4 617,865 - BBR_RS12775 0.51 -0.0 617,928 + BBR_RS12775 0.66 -0.3 617,928 + BBR_RS12775 0.66 +0.5 617,928 + BBR_RS12775 0.66 +0.2 617,928 + BBR_RS12775 0.66 +0.3 617,928 + BBR_RS12775 0.66 -1.0 617,936 + BBR_RS12775 0.68 -0.1 617,936 - BBR_RS12775 0.68 +2.4 617,936 - BBR_RS12775 0.68 -0.3 617,936 - BBR_RS12775 0.68 -1.0 618,019 + BBR_RS12775 0.88 +0.5 618,021 + BBR_RS12775 0.89 -0.1 618,038 + +0.0 618,055 + +0.1 618,110 - -0.3 618,115 - +0.3 618,157 - BBR_RS12780 0.17 +0.6 618,169 - BBR_RS12780 0.19 -1.0
Or see this region's nucleotide sequence