Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS23025 and ABZR87_RS23030 are separated by 39 nucleotides ABZR87_RS23030 and ABZR87_RS23035 overlap by 17 nucleotides ABZR87_RS23035 and ABZR87_RS23040 overlap by 4 nucleotides
ABZR87_RS23025: ABZR87_RS23025 - UDP-glucuronic acid decarboxylase family protein, at 1,525,732 to 1,526,754
_RS23025
ABZR87_RS23030: ABZR87_RS23030 - glycosyltransferase, at 1,526,794 to 1,527,717
_RS23030
ABZR87_RS23035: ABZR87_RS23035 - PIG-L family deacetylase, at 1,527,701 to 1,528,423
_RS23035
ABZR87_RS23040: ABZR87_RS23040 - zinc-dependent alcohol dehydrogenase, at 1,528,420 to 1,529,691
_RS23040
Position (kb)
1527
1528
1529 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1526.708 kb on - strand at 1526.708 kb on - strand at 1526.868 kb on + strand at 1526.869 kb on - strand at 1526.883 kb on + strand at 1527.113 kb on + strand, within ABZR87_RS23030 at 1527.113 kb on + strand, within ABZR87_RS23030 at 1527.114 kb on - strand, within ABZR87_RS23030 at 1527.191 kb on + strand, within ABZR87_RS23030 at 1527.208 kb on - strand, within ABZR87_RS23030 at 1527.391 kb on + strand, within ABZR87_RS23030 at 1527.392 kb on - strand, within ABZR87_RS23030 at 1527.392 kb on - strand, within ABZR87_RS23030 at 1527.449 kb on + strand, within ABZR87_RS23030 at 1527.449 kb on + strand, within ABZR87_RS23030 at 1527.450 kb on - strand, within ABZR87_RS23030 at 1527.498 kb on - strand, within ABZR87_RS23030 at 1527.656 kb on + strand at 1527.656 kb on + strand at 1527.656 kb on + strand at 1527.656 kb on + strand at 1527.656 kb on + strand at 1527.657 kb on - strand at 1527.817 kb on - strand, within ABZR87_RS23035 at 1527.943 kb on - strand, within ABZR87_RS23035 at 1528.090 kb on - strand, within ABZR87_RS23035 at 1528.614 kb on - strand, within ABZR87_RS23040 at 1528.702 kb on + strand, within ABZR87_RS23040 at 1528.702 kb on + strand, within ABZR87_RS23040 at 1528.703 kb on - strand, within ABZR87_RS23040 at 1528.703 kb on - strand, within ABZR87_RS23040 at 1528.703 kb on - strand, within ABZR87_RS23040 at 1529.189 kb on + strand, within ABZR87_RS23040 at 1529.189 kb on + strand, within ABZR87_RS23040 at 1529.394 kb on - strand, within ABZR87_RS23040 at 1529.408 kb on + strand, within ABZR87_RS23040 at 1529.408 kb on + strand, within ABZR87_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 1,526,708 - +0.8 1,526,708 - -0.8 1,526,868 + -1.0 1,526,869 - +2.5 1,526,883 + -0.9 1,527,113 + ABZR87_RS23030 0.35 +0.3 1,527,113 + ABZR87_RS23030 0.35 +1.4 1,527,114 - ABZR87_RS23030 0.35 +0.6 1,527,191 + ABZR87_RS23030 0.43 +0.3 1,527,208 - ABZR87_RS23030 0.45 -0.5 1,527,391 + ABZR87_RS23030 0.65 +0.3 1,527,392 - ABZR87_RS23030 0.65 -0.6 1,527,392 - ABZR87_RS23030 0.65 +0.7 1,527,449 + ABZR87_RS23030 0.71 +0.7 1,527,449 + ABZR87_RS23030 0.71 +2.3 1,527,450 - ABZR87_RS23030 0.71 -0.1 1,527,498 - ABZR87_RS23030 0.76 +0.1 1,527,656 + -1.1 1,527,656 + +0.6 1,527,656 + -1.7 1,527,656 + +1.2 1,527,656 + +0.5 1,527,657 - -0.3 1,527,817 - ABZR87_RS23035 0.16 +0.5 1,527,943 - ABZR87_RS23035 0.33 -0.4 1,528,090 - ABZR87_RS23035 0.54 +0.1 1,528,614 - ABZR87_RS23040 0.15 -1.5 1,528,702 + ABZR87_RS23040 0.22 -0.7 1,528,702 + ABZR87_RS23040 0.22 +0.6 1,528,703 - ABZR87_RS23040 0.22 -0.1 1,528,703 - ABZR87_RS23040 0.22 +1.0 1,528,703 - ABZR87_RS23040 0.22 +0.8 1,529,189 + ABZR87_RS23040 0.60 -2.3 1,529,189 + ABZR87_RS23040 0.60 -0.4 1,529,394 - ABZR87_RS23040 0.77 -1.3 1,529,408 + ABZR87_RS23040 0.78 +0.1 1,529,408 + ABZR87_RS23040 0.78 -2.3
Or see this region's nucleotide sequence