Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22100 and ABZR87_RS22105 overlap by 4 nucleotides ABZR87_RS22105 and ABZR87_RS22110 overlap by 4 nucleotides ABZR87_RS22110 and ABZR87_RS22115 overlap by 16 nucleotides ABZR87_RS22115 and ABZR87_RS22120 are separated by 2 nucleotides
ABZR87_RS22100: ABZR87_RS22100 - CDP-glucose 4,6-dehydratase, at 1,326,674 to 1,327,780
_RS22100
ABZR87_RS22105: ABZR87_RS22105 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,327,777 to 1,328,370
_RS22105
ABZR87_RS22110: ABZR87_RS22110 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,328,367 to 1,329,518
_RS22110
ABZR87_RS22115: ABZR87_RS22115 - phytanoyl-CoA dioxygenase family protein, at 1,329,503 to 1,330,345
_RS22115
ABZR87_RS22120: ABZR87_RS22120 - class I SAM-dependent methyltransferase, at 1,330,348 to 1,331,535
_RS22120
Position (kb)
1328
1329
1330 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1327.397 kb on + strand, within ABZR87_RS22100 at 1327.398 kb on - strand, within ABZR87_RS22100 at 1327.398 kb on - strand, within ABZR87_RS22100 at 1327.673 kb on + strand at 1327.838 kb on + strand, within ABZR87_RS22105 at 1328.179 kb on + strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.180 kb on - strand, within ABZR87_RS22105 at 1328.385 kb on + strand at 1328.422 kb on - strand at 1328.422 kb on - strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.463 kb on + strand at 1328.464 kb on - strand at 1328.469 kb on + strand at 1328.469 kb on + strand at 1328.470 kb on - strand at 1328.470 kb on - strand at 1328.492 kb on - strand, within ABZR87_RS22110 at 1328.517 kb on + strand, within ABZR87_RS22110 at 1328.517 kb on + strand, within ABZR87_RS22110 at 1328.518 kb on - strand, within ABZR87_RS22110 at 1328.518 kb on - strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.643 kb on + strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.644 kb on - strand, within ABZR87_RS22110 at 1328.761 kb on - strand, within ABZR87_RS22110 at 1328.970 kb on + strand, within ABZR87_RS22110 at 1328.970 kb on + strand, within ABZR87_RS22110 at 1328.971 kb on - strand, within ABZR87_RS22110 at 1328.971 kb on - strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.051 kb on + strand, within ABZR87_RS22110 at 1329.144 kb on + strand, within ABZR87_RS22110 at 1329.145 kb on - strand, within ABZR87_RS22110 at 1329.213 kb on + strand, within ABZR87_RS22110 at 1329.213 kb on + strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110 at 1329.214 kb on - strand, within ABZR87_RS22110 at 1329.385 kb on - strand, within ABZR87_RS22110 at 1329.385 kb on - strand, within ABZR87_RS22110 at 1329.385 kb on - strand, within ABZR87_RS22110 at 1329.576 kb on - strand at 1329.614 kb on + strand, within ABZR87_RS22115 at 1329.614 kb on + strand, within ABZR87_RS22115 at 1329.615 kb on - strand, within ABZR87_RS22115 at 1329.615 kb on - strand, within ABZR87_RS22115 at 1329.615 kb on - strand, within ABZR87_RS22115 at 1329.644 kb on + strand, within ABZR87_RS22115 at 1329.644 kb on + strand, within ABZR87_RS22115 at 1329.645 kb on - strand, within ABZR87_RS22115 at 1329.645 kb on - strand, within ABZR87_RS22115 at 1329.699 kb on - strand, within ABZR87_RS22115 at 1329.743 kb on + strand, within ABZR87_RS22115 at 1329.743 kb on + strand, within ABZR87_RS22115 at 1329.743 kb on + strand, within ABZR87_RS22115 at 1329.744 kb on - strand, within ABZR87_RS22115 at 1329.917 kb on + strand, within ABZR87_RS22115 at 1330.079 kb on + strand, within ABZR87_RS22115 at 1330.110 kb on - strand, within ABZR87_RS22115 at 1330.110 kb on - strand, within ABZR87_RS22115 at 1330.290 kb on - strand at 1330.290 kb on - strand at 1330.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 1,327,397 + ABZR87_RS22100 0.65 +1.0 1,327,398 - ABZR87_RS22100 0.65 -1.0 1,327,398 - ABZR87_RS22100 0.65 -0.9 1,327,673 + +0.2 1,327,838 + ABZR87_RS22105 0.10 +1.1 1,328,179 + ABZR87_RS22105 0.68 +0.8 1,328,180 - ABZR87_RS22105 0.68 +1.3 1,328,180 - ABZR87_RS22105 0.68 +2.0 1,328,180 - ABZR87_RS22105 0.68 +0.3 1,328,180 - ABZR87_RS22105 0.68 +0.1 1,328,385 + +0.0 1,328,422 - -2.0 1,328,422 - +1.4 1,328,463 + +2.9 1,328,463 + -0.1 1,328,463 + +0.5 1,328,463 + -0.6 1,328,464 - -0.8 1,328,469 + -0.6 1,328,469 + -0.2 1,328,470 - +0.1 1,328,470 - +0.2 1,328,492 - ABZR87_RS22110 0.11 +1.2 1,328,517 + ABZR87_RS22110 0.13 +0.1 1,328,517 + ABZR87_RS22110 0.13 +0.3 1,328,518 - ABZR87_RS22110 0.13 +0.0 1,328,518 - ABZR87_RS22110 0.13 -0.2 1,328,643 + ABZR87_RS22110 0.24 +0.4 1,328,643 + ABZR87_RS22110 0.24 +0.3 1,328,643 + ABZR87_RS22110 0.24 -0.4 1,328,643 + ABZR87_RS22110 0.24 -0.6 1,328,644 - ABZR87_RS22110 0.24 +1.2 1,328,644 - ABZR87_RS22110 0.24 +1.3 1,328,644 - ABZR87_RS22110 0.24 +1.0 1,328,761 - ABZR87_RS22110 0.34 -1.0 1,328,970 + ABZR87_RS22110 0.52 -0.5 1,328,970 + ABZR87_RS22110 0.52 -0.6 1,328,971 - ABZR87_RS22110 0.52 +0.6 1,328,971 - ABZR87_RS22110 0.52 -1.0 1,329,051 + ABZR87_RS22110 0.59 -1.7 1,329,051 + ABZR87_RS22110 0.59 -1.4 1,329,051 + ABZR87_RS22110 0.59 -0.5 1,329,144 + ABZR87_RS22110 0.67 -0.0 1,329,145 - ABZR87_RS22110 0.68 -1.8 1,329,213 + ABZR87_RS22110 0.73 +0.8 1,329,213 + ABZR87_RS22110 0.73 +0.5 1,329,214 - ABZR87_RS22110 0.74 -1.3 1,329,214 - ABZR87_RS22110 0.74 -0.9 1,329,214 - ABZR87_RS22110 0.74 +1.9 1,329,385 - ABZR87_RS22110 0.88 -0.2 1,329,385 - ABZR87_RS22110 0.88 +2.6 1,329,385 - ABZR87_RS22110 0.88 -0.2 1,329,576 - -1.9 1,329,614 + ABZR87_RS22115 0.13 -1.9 1,329,614 + ABZR87_RS22115 0.13 +1.2 1,329,615 - ABZR87_RS22115 0.13 +2.0 1,329,615 - ABZR87_RS22115 0.13 -1.5 1,329,615 - ABZR87_RS22115 0.13 +0.1 1,329,644 + ABZR87_RS22115 0.17 +0.5 1,329,644 + ABZR87_RS22115 0.17 +0.5 1,329,645 - ABZR87_RS22115 0.17 +0.1 1,329,645 - ABZR87_RS22115 0.17 -0.3 1,329,699 - ABZR87_RS22115 0.23 +0.2 1,329,743 + ABZR87_RS22115 0.28 -0.3 1,329,743 + ABZR87_RS22115 0.28 +0.0 1,329,743 + ABZR87_RS22115 0.28 -0.3 1,329,744 - ABZR87_RS22115 0.29 -0.2 1,329,917 + ABZR87_RS22115 0.49 -0.1 1,330,079 + ABZR87_RS22115 0.68 +0.3 1,330,110 - ABZR87_RS22115 0.72 -0.9 1,330,110 - ABZR87_RS22115 0.72 -0.1 1,330,290 - -0.3 1,330,290 - +0.2 1,330,406 - +0.0
Or see this region's nucleotide sequence