Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS20445 and ABZR87_RS20450 are separated by 45 nucleotides ABZR87_RS20450 and ABZR87_RS20455 are separated by 59 nucleotides
ABZR87_RS20445: ABZR87_RS20445 - LysR substrate-binding domain-containing protein, at 974,725 to 975,660
_RS20445
ABZR87_RS20450: ABZR87_RS20450 - HAD family phosphatase, at 975,706 to 976,389
_RS20450
ABZR87_RS20455: ABZR87_RS20455 - TIGR03364 family FAD-dependent oxidoreductase, at 976,449 to 977,669
_RS20455
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 974.738 kb on + strand at 974.738 kb on + strand at 974.738 kb on + strand at 974.738 kb on + strand at 974.738 kb on + strand at 974.738 kb on + strand at 974.738 kb on + strand at 974.739 kb on - strand at 974.739 kb on - strand at 974.739 kb on - strand at 974.739 kb on - strand at 974.739 kb on - strand at 974.739 kb on - strand at 974.851 kb on + strand, within ABZR87_RS20445 at 974.851 kb on + strand, within ABZR87_RS20445 at 974.851 kb on + strand, within ABZR87_RS20445 at 974.852 kb on - strand, within ABZR87_RS20445 at 974.852 kb on - strand, within ABZR87_RS20445 at 974.852 kb on - strand, within ABZR87_RS20445 at 974.852 kb on - strand, within ABZR87_RS20445 at 974.852 kb on - strand, within ABZR87_RS20445 at 975.025 kb on + strand, within ABZR87_RS20445 at 975.025 kb on + strand, within ABZR87_RS20445 at 975.025 kb on + strand, within ABZR87_RS20445 at 975.026 kb on - strand, within ABZR87_RS20445 at 975.026 kb on - strand, within ABZR87_RS20445 at 975.026 kb on - strand, within ABZR87_RS20445 at 975.026 kb on - strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.028 kb on + strand, within ABZR87_RS20445 at 975.029 kb on - strand, within ABZR87_RS20445 at 975.029 kb on - strand, within ABZR87_RS20445 at 975.259 kb on - strand, within ABZR87_RS20445 at 975.397 kb on + strand, within ABZR87_RS20445 at 975.397 kb on + strand, within ABZR87_RS20445 at 975.511 kb on + strand, within ABZR87_RS20445 at 975.517 kb on + strand, within ABZR87_RS20445 at 975.518 kb on - strand, within ABZR87_RS20445 at 975.518 kb on - strand, within ABZR87_RS20445 at 975.630 kb on + strand at 975.659 kb on - strand at 975.705 kb on + strand at 975.706 kb on - strand at 975.706 kb on - strand at 975.706 kb on - strand at 976.054 kb on + strand, within ABZR87_RS20450 at 976.259 kb on - strand, within ABZR87_RS20450 at 976.526 kb on + strand at 976.527 kb on - strand at 976.527 kb on - strand at 976.527 kb on - strand at 976.912 kb on + strand, within ABZR87_RS20455 at 976.912 kb on + strand, within ABZR87_RS20455 at 976.912 kb on + strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 976.913 kb on - strand, within ABZR87_RS20455 at 977.044 kb on + strand, within ABZR87_RS20455 at 977.204 kb on - strand, within ABZR87_RS20455 at 977.263 kb on + strand, within ABZR87_RS20455 at 977.263 kb on + strand, within ABZR87_RS20455 at 977.263 kb on + strand, within ABZR87_RS20455 at 977.264 kb on - strand, within ABZR87_RS20455
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 974,738 + +1.2 974,738 + -1.0 974,738 + -0.6 974,738 + -0.5 974,738 + -0.3 974,738 + -0.9 974,738 + -1.0 974,739 - -1.2 974,739 - -2.2 974,739 - -0.5 974,739 - +1.4 974,739 - -0.8 974,739 - -1.5 974,851 + ABZR87_RS20445 0.13 +0.1 974,851 + ABZR87_RS20445 0.13 -0.5 974,851 + ABZR87_RS20445 0.13 +2.3 974,852 - ABZR87_RS20445 0.14 +0.7 974,852 - ABZR87_RS20445 0.14 -1.0 974,852 - ABZR87_RS20445 0.14 -2.4 974,852 - ABZR87_RS20445 0.14 +0.5 974,852 - ABZR87_RS20445 0.14 +1.8 975,025 + ABZR87_RS20445 0.32 -0.5 975,025 + ABZR87_RS20445 0.32 +1.3 975,025 + ABZR87_RS20445 0.32 -0.0 975,026 - ABZR87_RS20445 0.32 -1.4 975,026 - ABZR87_RS20445 0.32 -0.1 975,026 - ABZR87_RS20445 0.32 -0.9 975,026 - ABZR87_RS20445 0.32 +1.0 975,028 + ABZR87_RS20445 0.32 +0.3 975,028 + ABZR87_RS20445 0.32 -2.6 975,028 + ABZR87_RS20445 0.32 -1.5 975,028 + ABZR87_RS20445 0.32 +1.4 975,028 + ABZR87_RS20445 0.32 -1.5 975,028 + ABZR87_RS20445 0.32 +1.4 975,028 + ABZR87_RS20445 0.32 +0.4 975,029 - ABZR87_RS20445 0.32 +1.5 975,029 - ABZR87_RS20445 0.32 -1.7 975,259 - ABZR87_RS20445 0.57 +0.0 975,397 + ABZR87_RS20445 0.72 -0.1 975,397 + ABZR87_RS20445 0.72 +0.5 975,511 + ABZR87_RS20445 0.84 +0.1 975,517 + ABZR87_RS20445 0.85 +0.7 975,518 - ABZR87_RS20445 0.85 -0.8 975,518 - ABZR87_RS20445 0.85 +1.9 975,630 + -0.4 975,659 - -1.3 975,705 + -0.2 975,706 - -0.5 975,706 - +0.4 975,706 - +0.2 976,054 + ABZR87_RS20450 0.51 +0.2 976,259 - ABZR87_RS20450 0.81 +0.2 976,526 + +0.2 976,527 - +2.7 976,527 - -1.8 976,527 - -0.6 976,912 + ABZR87_RS20455 0.38 -0.9 976,912 + ABZR87_RS20455 0.38 +0.3 976,912 + ABZR87_RS20455 0.38 +0.2 976,913 - ABZR87_RS20455 0.38 +0.2 976,913 - ABZR87_RS20455 0.38 -2.8 976,913 - ABZR87_RS20455 0.38 +0.4 976,913 - ABZR87_RS20455 0.38 -0.1 976,913 - ABZR87_RS20455 0.38 -0.8 976,913 - ABZR87_RS20455 0.38 -0.4 977,044 + ABZR87_RS20455 0.49 -0.0 977,204 - ABZR87_RS20455 0.62 +0.3 977,263 + ABZR87_RS20455 0.67 -0.7 977,263 + ABZR87_RS20455 0.67 -2.7 977,263 + ABZR87_RS20455 0.67 -0.9 977,264 - ABZR87_RS20455 0.67 -1.0
Or see this region's nucleotide sequence