Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18225 and ABZR87_RS18230 overlap by 4 nucleotides ABZR87_RS18230 and ABZR87_RS18235 are separated by 46 nucleotides ABZR87_RS18235 and ABZR87_RS18240 overlap by 4 nucleotides
ABZR87_RS18225: ABZR87_RS18225 - D-xylose ABC transporter ATP-binding protein, at 459,247 to 460,830
_RS18225
ABZR87_RS18230: ABZR87_RS18230 - sugar ABC transporter permease, at 460,827 to 462,029
_RS18230
ABZR87_RS18235: ABZR87_RS18235 - SDR family oxidoreductase, at 462,076 to 462,843
_RS18235
ABZR87_RS18240: ABZR87_RS18240 - SMP-30/gluconolactonase/LRE family protein, at 462,840 to 463,745
_RS18240
Position (kb) 460
461
462
463 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 459.844 kb on + strand, within ABZR87_RS18225 at 459.844 kb on + strand, within ABZR87_RS18225 at 459.844 kb on + strand, within ABZR87_RS18225 at 459.844 kb on + strand, within ABZR87_RS18225 at 459.844 kb on + strand, within ABZR87_RS18225 at 460.055 kb on - strand, within ABZR87_RS18225 at 460.186 kb on + strand, within ABZR87_RS18225 at 460.577 kb on - strand, within ABZR87_RS18225 at 460.585 kb on + strand, within ABZR87_RS18225 at 460.586 kb on - strand, within ABZR87_RS18225 at 460.586 kb on - strand, within ABZR87_RS18225 at 460.586 kb on - strand, within ABZR87_RS18225 at 460.608 kb on + strand, within ABZR87_RS18225 at 460.609 kb on - strand, within ABZR87_RS18225 at 460.881 kb on - strand at 460.923 kb on + strand at 461.152 kb on + strand, within ABZR87_RS18230 at 461.371 kb on - strand, within ABZR87_RS18230 at 461.371 kb on - strand, within ABZR87_RS18230 at 461.554 kb on + strand, within ABZR87_RS18230 at 461.556 kb on + strand, within ABZR87_RS18230 at 461.557 kb on - strand, within ABZR87_RS18230 at 461.557 kb on - strand, within ABZR87_RS18230 at 461.557 kb on - strand, within ABZR87_RS18230 at 461.645 kb on + strand, within ABZR87_RS18230 at 461.646 kb on - strand, within ABZR87_RS18230 at 461.649 kb on + strand, within ABZR87_RS18230 at 461.649 kb on + strand, within ABZR87_RS18230 at 461.649 kb on + strand, within ABZR87_RS18230 at 461.877 kb on + strand, within ABZR87_RS18230 at 461.949 kb on + strand at 461.949 kb on + strand at 462.038 kb on + strand at 462.038 kb on + strand at 462.038 kb on + strand at 462.039 kb on - strand at 462.075 kb on + strand at 462.076 kb on - strand at 462.076 kb on - strand at 462.076 kb on - strand at 462.083 kb on - strand at 462.083 kb on - strand at 462.289 kb on - strand, within ABZR87_RS18235 at 462.553 kb on + strand, within ABZR87_RS18235 at 462.553 kb on + strand, within ABZR87_RS18235 at 462.553 kb on + strand, within ABZR87_RS18235 at 462.554 kb on - strand, within ABZR87_RS18235 at 462.554 kb on - strand, within ABZR87_RS18235 at 462.554 kb on - strand, within ABZR87_RS18235 at 462.812 kb on - strand at 462.833 kb on - strand at 462.961 kb on - strand, within ABZR87_RS18240 at 462.966 kb on + strand, within ABZR87_RS18240 at 462.967 kb on - strand, within ABZR87_RS18240
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 459,844 + ABZR87_RS18225 0.38 +1.4 459,844 + ABZR87_RS18225 0.38 -1.3 459,844 + ABZR87_RS18225 0.38 -2.9 459,844 + ABZR87_RS18225 0.38 -0.9 459,844 + ABZR87_RS18225 0.38 +2.3 460,055 - ABZR87_RS18225 0.51 -0.5 460,186 + ABZR87_RS18225 0.59 -1.2 460,577 - ABZR87_RS18225 0.84 +0.7 460,585 + ABZR87_RS18225 0.84 -1.8 460,586 - ABZR87_RS18225 0.85 +0.4 460,586 - ABZR87_RS18225 0.85 +1.1 460,586 - ABZR87_RS18225 0.85 -1.8 460,608 + ABZR87_RS18225 0.86 -0.0 460,609 - ABZR87_RS18225 0.86 -0.4 460,881 - +1.4 460,923 + +1.0 461,152 + ABZR87_RS18230 0.27 +0.6 461,371 - ABZR87_RS18230 0.45 -3.0 461,371 - ABZR87_RS18230 0.45 -1.0 461,554 + ABZR87_RS18230 0.60 +0.3 461,556 + ABZR87_RS18230 0.61 -0.8 461,557 - ABZR87_RS18230 0.61 -1.6 461,557 - ABZR87_RS18230 0.61 -2.9 461,557 - ABZR87_RS18230 0.61 -1.8 461,645 + ABZR87_RS18230 0.68 +2.2 461,646 - ABZR87_RS18230 0.68 -0.8 461,649 + ABZR87_RS18230 0.68 -0.5 461,649 + ABZR87_RS18230 0.68 -0.5 461,649 + ABZR87_RS18230 0.68 +1.6 461,877 + ABZR87_RS18230 0.87 -1.5 461,949 + -0.7 461,949 + -0.7 462,038 + +1.2 462,038 + +1.3 462,038 + +1.3 462,039 - -0.3 462,075 + +1.3 462,076 - +0.3 462,076 - -0.1 462,076 - +1.2 462,083 - +1.4 462,083 - -0.8 462,289 - ABZR87_RS18235 0.28 -0.1 462,553 + ABZR87_RS18235 0.62 +0.0 462,553 + ABZR87_RS18235 0.62 -0.5 462,553 + ABZR87_RS18235 0.62 -0.1 462,554 - ABZR87_RS18235 0.62 +1.4 462,554 - ABZR87_RS18235 0.62 +0.3 462,554 - ABZR87_RS18235 0.62 -0.4 462,812 - -0.1 462,833 - +0.5 462,961 - ABZR87_RS18240 0.13 +0.6 462,966 + ABZR87_RS18240 0.14 -0.7 462,967 - ABZR87_RS18240 0.14 +0.1
Or see this region's nucleotide sequence