Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16720 and ABZR87_RS16725 are separated by 162 nucleotides ABZR87_RS16725 and ABZR87_RS16730 are separated by 82 nucleotides ABZR87_RS16730 and ABZR87_RS16735 overlap by 4 nucleotides
ABZR87_RS16720: ABZR87_RS16720 - collagen-like triple helix repeat-containing protein, at 100,155 to 101,300
_RS16720
ABZR87_RS16725: ABZR87_RS16725 - antibiotic biosynthesis monooxygenase, at 101,463 to 101,771
_RS16725
ABZR87_RS16730: ABZR87_RS16730 - 2-methylaconitate cis-trans isomerase PrpF, at 101,854 to 103,038
_RS16730
ABZR87_RS16735: ABZR87_RS16735 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 103,035 to 105,632
_RS16735
Position (kb) 101
102
103
104 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 100.913 kb on + strand, within ABZR87_RS16720 at 100.914 kb on - strand, within ABZR87_RS16720 at 101.377 kb on + strand at 101.377 kb on + strand at 101.377 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.389 kb on + strand at 101.390 kb on - strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.415 kb on + strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.416 kb on - strand at 101.420 kb on + strand at 101.420 kb on + strand at 101.421 kb on - strand at 101.421 kb on - strand at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.173 kb on + strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.174 kb on - strand, within ABZR87_RS16730 at 102.345 kb on - strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.782 kb on + strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 102.783 kb on - strand, within ABZR87_RS16730 at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.001 kb on + strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.002 kb on - strand at 103.103 kb on - strand at 103.103 kb on - strand at 103.408 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.453 kb on + strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.454 kb on - strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.618 kb on + strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.619 kb on - strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.660 kb on + strand, within ABZR87_RS16735 at 103.792 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.810 kb on + strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.811 kb on - strand, within ABZR87_RS16735 at 103.835 kb on - strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.888 kb on + strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.889 kb on - strand, within ABZR87_RS16735 at 103.890 kb on + strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735 at 103.891 kb on - strand, within ABZR87_RS16735
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 100,913 + ABZR87_RS16720 0.66 -0.7 100,914 - ABZR87_RS16720 0.66 +0.9 101,377 + +0.5 101,377 + +2.6 101,377 + -0.9 101,389 + +0.5 101,389 + +0.2 101,389 + -1.1 101,390 - +1.4 101,415 + +0.8 101,415 + -0.9 101,415 + -1.9 101,415 + +1.3 101,416 - +0.1 101,416 - -0.0 101,416 - -0.3 101,416 - -0.4 101,416 - +0.8 101,416 - -3.0 101,420 + -2.0 101,420 + -0.9 101,421 - -2.4 101,421 - +0.1 102,173 + ABZR87_RS16730 0.27 +2.1 102,173 + ABZR87_RS16730 0.27 -2.2 102,173 + ABZR87_RS16730 0.27 -0.2 102,174 - ABZR87_RS16730 0.27 -2.1 102,174 - ABZR87_RS16730 0.27 -3.4 102,174 - ABZR87_RS16730 0.27 -1.9 102,345 - ABZR87_RS16730 0.41 -0.8 102,782 + ABZR87_RS16730 0.78 -2.6 102,782 + ABZR87_RS16730 0.78 -1.8 102,783 - ABZR87_RS16730 0.78 -0.9 102,783 - ABZR87_RS16730 0.78 +2.3 102,783 - ABZR87_RS16730 0.78 +1.4 102,783 - ABZR87_RS16730 0.78 -1.9 103,001 + -2.3 103,001 + -0.4 103,001 + -2.9 103,001 + -1.4 103,001 + -1.9 103,001 + +0.1 103,001 + +0.1 103,002 - -1.1 103,002 - -2.5 103,002 - -1.3 103,002 - -2.7 103,002 - -1.9 103,103 - -1.2 103,103 - -3.9 103,408 + ABZR87_RS16735 0.14 -1.1 103,453 + ABZR87_RS16735 0.16 -3.4 103,453 + ABZR87_RS16735 0.16 +0.6 103,453 + ABZR87_RS16735 0.16 +0.0 103,453 + ABZR87_RS16735 0.16 +0.8 103,454 - ABZR87_RS16735 0.16 -1.2 103,454 - ABZR87_RS16735 0.16 -2.1 103,454 - ABZR87_RS16735 0.16 -0.9 103,618 + ABZR87_RS16735 0.22 -1.9 103,618 + ABZR87_RS16735 0.22 -3.0 103,619 - ABZR87_RS16735 0.22 -0.1 103,619 - ABZR87_RS16735 0.22 -1.0 103,660 + ABZR87_RS16735 0.24 -0.8 103,660 + ABZR87_RS16735 0.24 -2.6 103,660 + ABZR87_RS16735 0.24 +4.8 103,660 + ABZR87_RS16735 0.24 +0.7 103,792 + ABZR87_RS16735 0.29 -0.9 103,810 + ABZR87_RS16735 0.30 -1.4 103,810 + ABZR87_RS16735 0.30 -3.2 103,810 + ABZR87_RS16735 0.30 +1.3 103,810 + ABZR87_RS16735 0.30 -2.2 103,810 + ABZR87_RS16735 0.30 -2.6 103,810 + ABZR87_RS16735 0.30 -1.4 103,810 + ABZR87_RS16735 0.30 -2.6 103,811 - ABZR87_RS16735 0.30 -1.9 103,811 - ABZR87_RS16735 0.30 -1.4 103,811 - ABZR87_RS16735 0.30 -1.8 103,811 - ABZR87_RS16735 0.30 -0.5 103,811 - ABZR87_RS16735 0.30 +1.2 103,811 - ABZR87_RS16735 0.30 -2.1 103,835 - ABZR87_RS16735 0.31 -2.0 103,888 + ABZR87_RS16735 0.33 -2.6 103,888 + ABZR87_RS16735 0.33 -2.0 103,888 + ABZR87_RS16735 0.33 -1.9 103,888 + ABZR87_RS16735 0.33 -0.9 103,888 + ABZR87_RS16735 0.33 -2.2 103,888 + ABZR87_RS16735 0.33 -1.7 103,888 + ABZR87_RS16735 0.33 -2.9 103,888 + ABZR87_RS16735 0.33 -3.3 103,889 - ABZR87_RS16735 0.33 -1.6 103,889 - ABZR87_RS16735 0.33 -1.5 103,889 - ABZR87_RS16735 0.33 +0.0 103,889 - ABZR87_RS16735 0.33 -2.4 103,889 - ABZR87_RS16735 0.33 -0.1 103,889 - ABZR87_RS16735 0.33 +0.3 103,889 - ABZR87_RS16735 0.33 -1.6 103,890 + ABZR87_RS16735 0.33 -3.3 103,891 - ABZR87_RS16735 0.33 -2.7 103,891 - ABZR87_RS16735 0.33 +1.5
Or see this region's nucleotide sequence