Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16540 and ABZR87_RS16545 are separated by 17 nucleotides ABZR87_RS16545 and ABZR87_RS16550 are separated by 1 nucleotides ABZR87_RS16550 and ABZR87_RS16555 are separated by 37 nucleotides
ABZR87_RS16540: ABZR87_RS16540 - MarR family transcriptional regulator, at 61,344 to 61,928
_RS16540
ABZR87_RS16545: ABZR87_RS16545 - enoyl-CoA hydratase family protein, at 61,946 to 62,806
_RS16545
ABZR87_RS16550: ABZR87_RS16550 - acyl-CoA dehydrogenase family protein, at 62,808 to 63,983
_RS16550
ABZR87_RS16555: ABZR87_RS16555 - AMP-binding protein, at 64,021 to 65,637
_RS16555
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 61.851 kb on + strand, within ABZR87_RS16540 at 61.852 kb on - strand, within ABZR87_RS16540 at 62.108 kb on + strand, within ABZR87_RS16545 at 62.510 kb on + strand, within ABZR87_RS16545 at 62.511 kb on - strand, within ABZR87_RS16545 at 62.759 kb on + strand at 62.759 kb on + strand at 62.759 kb on + strand at 62.759 kb on + strand at 62.760 kb on - strand at 62.760 kb on - strand at 62.824 kb on - strand at 62.824 kb on - strand at 63.157 kb on - strand, within ABZR87_RS16550 at 63.157 kb on - strand, within ABZR87_RS16550 at 63.157 kb on - strand, within ABZR87_RS16550 at 63.270 kb on + strand, within ABZR87_RS16550 at 63.271 kb on - strand, within ABZR87_RS16550 at 63.271 kb on - strand, within ABZR87_RS16550 at 63.271 kb on - strand, within ABZR87_RS16550 at 63.271 kb on - strand, within ABZR87_RS16550 at 63.324 kb on + strand, within ABZR87_RS16550 at 63.324 kb on + strand, within ABZR87_RS16550 at 63.325 kb on - strand, within ABZR87_RS16550 at 64.036 kb on - strand at 64.087 kb on + strand at 64.216 kb on + strand, within ABZR87_RS16555 at 64.217 kb on - strand, within ABZR87_RS16555 at 64.217 kb on - strand, within ABZR87_RS16555 at 64.217 kb on - strand, within ABZR87_RS16555 at 64.217 kb on - strand, within ABZR87_RS16555 at 64.575 kb on + strand, within ABZR87_RS16555 at 64.575 kb on + strand, within ABZR87_RS16555 at 64.575 kb on + strand, within ABZR87_RS16555 at 64.576 kb on - strand, within ABZR87_RS16555 at 64.587 kb on + strand, within ABZR87_RS16555 at 64.758 kb on + strand, within ABZR87_RS16555 at 64.759 kb on - strand, within ABZR87_RS16555 at 64.759 kb on - strand, within ABZR87_RS16555 at 64.901 kb on - strand, within ABZR87_RS16555
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 61,851 + ABZR87_RS16540 0.87 +0.6 61,852 - ABZR87_RS16540 0.87 -2.9 62,108 + ABZR87_RS16545 0.19 -0.1 62,510 + ABZR87_RS16545 0.66 -0.7 62,511 - ABZR87_RS16545 0.66 +0.6 62,759 + -0.0 62,759 + -0.1 62,759 + -3.2 62,759 + +0.2 62,760 - -1.0 62,760 - -1.2 62,824 - -1.7 62,824 - +1.3 63,157 - ABZR87_RS16550 0.30 +0.2 63,157 - ABZR87_RS16550 0.30 -0.6 63,157 - ABZR87_RS16550 0.30 -0.8 63,270 + ABZR87_RS16550 0.39 -0.0 63,271 - ABZR87_RS16550 0.39 -0.2 63,271 - ABZR87_RS16550 0.39 -3.0 63,271 - ABZR87_RS16550 0.39 -1.5 63,271 - ABZR87_RS16550 0.39 -0.4 63,324 + ABZR87_RS16550 0.44 -0.3 63,324 + ABZR87_RS16550 0.44 -1.6 63,325 - ABZR87_RS16550 0.44 +1.5 64,036 - -0.3 64,087 + -1.0 64,216 + ABZR87_RS16555 0.12 +1.3 64,217 - ABZR87_RS16555 0.12 -0.3 64,217 - ABZR87_RS16555 0.12 +0.7 64,217 - ABZR87_RS16555 0.12 +0.0 64,217 - ABZR87_RS16555 0.12 +0.7 64,575 + ABZR87_RS16555 0.34 -1.6 64,575 + ABZR87_RS16555 0.34 +0.3 64,575 + ABZR87_RS16555 0.34 -0.6 64,576 - ABZR87_RS16555 0.34 +0.0 64,587 + ABZR87_RS16555 0.35 +0.2 64,758 + ABZR87_RS16555 0.46 +0.7 64,759 - ABZR87_RS16555 0.46 -2.6 64,759 - ABZR87_RS16555 0.46 +0.3 64,901 - ABZR87_RS16555 0.54 +1.7
Or see this region's nucleotide sequence