Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16125 and ABZR87_RS16130 are separated by 2 nucleotides ABZR87_RS16130 and ABZR87_RS16135 are separated by 15 nucleotides
ABZR87_RS16125: ABZR87_RS16125 - heme biosynthesis protein HemY, at 3,408,610 to 3,409,803
_RS16125
ABZR87_RS16130: ABZR87_RS16130 - fused uroporphyrinogen-III synthase HemD/membrane protein HemX, at 3,409,806 to 3,411,920
_RS16130
ABZR87_RS16135: ABZR87_RS16135 - hydroxymethylbilane synthase, at 3,411,936 to 3,412,952
_RS16135
Position (kb)
3409
3410
3411
3412 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3408.818 kb on + strand, within ABZR87_RS16125 at 3408.818 kb on + strand, within ABZR87_RS16125 at 3408.818 kb on + strand, within ABZR87_RS16125 at 3408.911 kb on + strand, within ABZR87_RS16125 at 3409.128 kb on + strand, within ABZR87_RS16125 at 3409.129 kb on - strand, within ABZR87_RS16125 at 3409.129 kb on - strand, within ABZR87_RS16125 at 3409.581 kb on - strand, within ABZR87_RS16125 at 3409.603 kb on - strand, within ABZR87_RS16125 at 3409.744 kb on - strand at 3409.792 kb on + strand at 3409.792 kb on + strand at 3410.170 kb on - strand, within ABZR87_RS16130 at 3410.324 kb on + strand, within ABZR87_RS16130 at 3410.324 kb on + strand, within ABZR87_RS16130 at 3410.324 kb on + strand, within ABZR87_RS16130 at 3410.377 kb on - strand, within ABZR87_RS16130 at 3410.642 kb on + strand, within ABZR87_RS16130 at 3411.002 kb on + strand, within ABZR87_RS16130 at 3411.002 kb on + strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.003 kb on - strand, within ABZR87_RS16130 at 3411.938 kb on - strand at 3411.938 kb on - strand at 3411.942 kb on - strand at 3411.942 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 3,408,818 + ABZR87_RS16125 0.17 -1.6 3,408,818 + ABZR87_RS16125 0.17 -3.7 3,408,818 + ABZR87_RS16125 0.17 -1.1 3,408,911 + ABZR87_RS16125 0.25 -3.3 3,409,128 + ABZR87_RS16125 0.43 -3.1 3,409,129 - ABZR87_RS16125 0.43 -3.6 3,409,129 - ABZR87_RS16125 0.43 -1.0 3,409,581 - ABZR87_RS16125 0.81 +0.5 3,409,603 - ABZR87_RS16125 0.83 -3.3 3,409,744 - -1.7 3,409,792 + -2.0 3,409,792 + -1.0 3,410,170 - ABZR87_RS16130 0.17 -1.8 3,410,324 + ABZR87_RS16130 0.24 -1.8 3,410,324 + ABZR87_RS16130 0.24 -2.3 3,410,324 + ABZR87_RS16130 0.24 -2.1 3,410,377 - ABZR87_RS16130 0.27 -3.2 3,410,642 + ABZR87_RS16130 0.40 -2.1 3,411,002 + ABZR87_RS16130 0.57 -1.1 3,411,002 + ABZR87_RS16130 0.57 -2.1 3,411,003 - ABZR87_RS16130 0.57 -0.3 3,411,003 - ABZR87_RS16130 0.57 -1.3 3,411,003 - ABZR87_RS16130 0.57 -0.1 3,411,003 - ABZR87_RS16130 0.57 -0.6 3,411,003 - ABZR87_RS16130 0.57 -0.6 3,411,003 - ABZR87_RS16130 0.57 -0.1 3,411,938 - -1.8 3,411,938 - +0.4 3,411,942 - -0.0 3,411,942 - -0.9
Or see this region's nucleotide sequence