Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS12820 and ABZR87_RS12825 are separated by 18 nucleotides ABZR87_RS12825 and ABZR87_RS12830 overlap by 11 nucleotides ABZR87_RS12830 and ABZR87_RS12835 overlap by 4 nucleotides
ABZR87_RS12820: ABZR87_RS12820 - IucA/IucC family siderophore biosynthesis protein, at 2,725,820 to 2,727,706
_RS12820
ABZR87_RS12825: ABZR87_RS12825 - GNAT family N-acetyltransferase, at 2,727,725 to 2,728,339
_RS12825
ABZR87_RS12830: ABZR87_RS12830 - MFS transporter, at 2,728,329 to 2,729,567
_RS12830
ABZR87_RS12835: ABZR87_RS12835 - SidA/IucD/PvdA family monooxygenase, at 2,729,564 to 2,731,036
_RS12835
Position (kb)
2728
2729
2730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2727.333 kb on + strand, within ABZR87_RS12820 at 2727.334 kb on - strand, within ABZR87_RS12820 at 2727.334 kb on - strand, within ABZR87_RS12820 at 2727.493 kb on - strand, within ABZR87_RS12820 at 2727.493 kb on - strand, within ABZR87_RS12820 at 2727.525 kb on + strand at 2727.588 kb on + strand at 2727.588 kb on + strand at 2727.608 kb on + strand at 2727.727 kb on - strand at 2727.761 kb on - strand at 2727.834 kb on + strand, within ABZR87_RS12825 at 2727.835 kb on - strand, within ABZR87_RS12825 at 2728.032 kb on + strand, within ABZR87_RS12825 at 2728.033 kb on - strand, within ABZR87_RS12825 at 2728.033 kb on - strand, within ABZR87_RS12825 at 2728.065 kb on + strand, within ABZR87_RS12825 at 2728.065 kb on + strand, within ABZR87_RS12825 at 2728.065 kb on + strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.066 kb on - strand, within ABZR87_RS12825 at 2728.110 kb on + strand, within ABZR87_RS12825 at 2728.146 kb on + strand, within ABZR87_RS12825 at 2728.146 kb on + strand, within ABZR87_RS12825 at 2728.146 kb on + strand, within ABZR87_RS12825 at 2728.147 kb on - strand, within ABZR87_RS12825 at 2728.147 kb on - strand, within ABZR87_RS12825 at 2728.266 kb on + strand, within ABZR87_RS12825 at 2728.561 kb on + strand, within ABZR87_RS12830 at 2728.561 kb on + strand, within ABZR87_RS12830 at 2728.562 kb on - strand, within ABZR87_RS12830 at 2728.562 kb on - strand, within ABZR87_RS12830 at 2728.562 kb on - strand, within ABZR87_RS12830 at 2728.562 kb on - strand, within ABZR87_RS12830 at 2728.574 kb on - strand, within ABZR87_RS12830 at 2728.645 kb on + strand, within ABZR87_RS12830 at 2728.646 kb on - strand, within ABZR87_RS12830 at 2728.867 kb on + strand, within ABZR87_RS12830 at 2728.867 kb on + strand, within ABZR87_RS12830 at 2728.868 kb on - strand, within ABZR87_RS12830 at 2728.868 kb on - strand, within ABZR87_RS12830 at 2728.895 kb on - strand, within ABZR87_RS12830 at 2729.042 kb on - strand, within ABZR87_RS12830 at 2729.042 kb on - strand, within ABZR87_RS12830 at 2729.225 kb on - strand, within ABZR87_RS12830 at 2729.225 kb on - strand, within ABZR87_RS12830 at 2729.225 kb on - strand, within ABZR87_RS12830 at 2729.225 kb on - strand, within ABZR87_RS12830 at 2729.225 kb on - strand, within ABZR87_RS12830 at 2729.230 kb on + strand, within ABZR87_RS12830 at 2729.231 kb on - strand, within ABZR87_RS12830 at 2729.242 kb on + strand, within ABZR87_RS12830 at 2729.242 kb on + strand, within ABZR87_RS12830 at 2729.284 kb on + strand, within ABZR87_RS12830 at 2729.347 kb on + strand, within ABZR87_RS12830 at 2729.437 kb on + strand, within ABZR87_RS12830 at 2729.437 kb on + strand, within ABZR87_RS12830 at 2729.438 kb on - strand, within ABZR87_RS12830 at 2729.438 kb on - strand, within ABZR87_RS12830 at 2729.438 kb on - strand, within ABZR87_RS12830 at 2729.470 kb on + strand at 2729.495 kb on - strand at 2729.495 kb on - strand at 2729.551 kb on + strand at 2729.551 kb on + strand at 2729.552 kb on - strand at 2729.552 kb on - strand at 2729.552 kb on - strand at 2729.552 kb on - strand at 2729.552 kb on - strand at 2729.552 kb on - strand at 2729.713 kb on - strand, within ABZR87_RS12835 at 2729.713 kb on - strand, within ABZR87_RS12835 at 2729.713 kb on - strand, within ABZR87_RS12835 at 2729.757 kb on + strand, within ABZR87_RS12835 at 2729.820 kb on + strand, within ABZR87_RS12835 at 2729.850 kb on + strand, within ABZR87_RS12835 at 2729.850 kb on + strand, within ABZR87_RS12835 at 2729.850 kb on + strand, within ABZR87_RS12835 at 2729.851 kb on - strand, within ABZR87_RS12835 at 2729.938 kb on - strand, within ABZR87_RS12835 at 2729.938 kb on - strand, within ABZR87_RS12835 at 2729.938 kb on - strand, within ABZR87_RS12835 at 2729.976 kb on + strand, within ABZR87_RS12835 at 2729.976 kb on + strand, within ABZR87_RS12835 at 2729.976 kb on + strand, within ABZR87_RS12835 at 2730.015 kb on + strand, within ABZR87_RS12835 at 2730.015 kb on + strand, within ABZR87_RS12835 at 2730.016 kb on - strand, within ABZR87_RS12835 at 2730.016 kb on - strand, within ABZR87_RS12835 at 2730.118 kb on - strand, within ABZR87_RS12835 at 2730.118 kb on - strand, within ABZR87_RS12835 at 2730.154 kb on - strand, within ABZR87_RS12835 at 2730.154 kb on - strand, within ABZR87_RS12835 at 2730.213 kb on + strand, within ABZR87_RS12835 at 2730.214 kb on - strand, within ABZR87_RS12835 at 2730.222 kb on + strand, within ABZR87_RS12835 at 2730.223 kb on - strand, within ABZR87_RS12835 at 2730.223 kb on - strand, within ABZR87_RS12835 at 2730.306 kb on + strand, within ABZR87_RS12835 at 2730.339 kb on + strand, within ABZR87_RS12835 at 2730.340 kb on - strand, within ABZR87_RS12835 at 2730.340 kb on - strand, within ABZR87_RS12835 at 2730.340 kb on - strand, within ABZR87_RS12835 at 2730.343 kb on - strand, within ABZR87_RS12835 at 2730.420 kb on + strand, within ABZR87_RS12835 at 2730.421 kb on - strand, within ABZR87_RS12835 at 2730.421 kb on - strand, within ABZR87_RS12835 at 2730.439 kb on - strand, within ABZR87_RS12835 at 2730.439 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835 at 2730.562 kb on - strand, within ABZR87_RS12835
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 2,727,333 + ABZR87_RS12820 0.80 -0.2 2,727,334 - ABZR87_RS12820 0.80 +1.6 2,727,334 - ABZR87_RS12820 0.80 +1.3 2,727,493 - ABZR87_RS12820 0.89 +0.6 2,727,493 - ABZR87_RS12820 0.89 +1.2 2,727,525 + +0.4 2,727,588 + +0.7 2,727,588 + +0.3 2,727,608 + -2.0 2,727,727 - -0.0 2,727,761 - +4.6 2,727,834 + ABZR87_RS12825 0.18 +1.3 2,727,835 - ABZR87_RS12825 0.18 +0.0 2,728,032 + ABZR87_RS12825 0.50 +0.8 2,728,033 - ABZR87_RS12825 0.50 -0.3 2,728,033 - ABZR87_RS12825 0.50 +1.8 2,728,065 + ABZR87_RS12825 0.55 -0.0 2,728,065 + ABZR87_RS12825 0.55 +0.2 2,728,065 + ABZR87_RS12825 0.55 +1.4 2,728,066 - ABZR87_RS12825 0.55 +0.9 2,728,066 - ABZR87_RS12825 0.55 +0.8 2,728,066 - ABZR87_RS12825 0.55 -1.6 2,728,066 - ABZR87_RS12825 0.55 +0.7 2,728,066 - ABZR87_RS12825 0.55 -0.0 2,728,066 - ABZR87_RS12825 0.55 -1.1 2,728,066 - ABZR87_RS12825 0.55 -1.0 2,728,066 - ABZR87_RS12825 0.55 -1.7 2,728,110 + ABZR87_RS12825 0.63 +1.8 2,728,146 + ABZR87_RS12825 0.68 -1.0 2,728,146 + ABZR87_RS12825 0.68 -0.7 2,728,146 + ABZR87_RS12825 0.68 +1.0 2,728,147 - ABZR87_RS12825 0.69 -0.5 2,728,147 - ABZR87_RS12825 0.69 -1.0 2,728,266 + ABZR87_RS12825 0.88 -0.2 2,728,561 + ABZR87_RS12830 0.19 +0.4 2,728,561 + ABZR87_RS12830 0.19 -1.7 2,728,562 - ABZR87_RS12830 0.19 +0.7 2,728,562 - ABZR87_RS12830 0.19 +0.4 2,728,562 - ABZR87_RS12830 0.19 -0.8 2,728,562 - ABZR87_RS12830 0.19 +0.5 2,728,574 - ABZR87_RS12830 0.20 -1.7 2,728,645 + ABZR87_RS12830 0.26 +0.2 2,728,646 - ABZR87_RS12830 0.26 -0.1 2,728,867 + ABZR87_RS12830 0.43 -1.8 2,728,867 + ABZR87_RS12830 0.43 -0.8 2,728,868 - ABZR87_RS12830 0.44 -0.9 2,728,868 - ABZR87_RS12830 0.44 -1.4 2,728,895 - ABZR87_RS12830 0.46 -0.7 2,729,042 - ABZR87_RS12830 0.58 +0.6 2,729,042 - ABZR87_RS12830 0.58 -3.0 2,729,225 - ABZR87_RS12830 0.72 +1.8 2,729,225 - ABZR87_RS12830 0.72 -0.8 2,729,225 - ABZR87_RS12830 0.72 -0.3 2,729,225 - ABZR87_RS12830 0.72 -0.0 2,729,225 - ABZR87_RS12830 0.72 -1.1 2,729,230 + ABZR87_RS12830 0.73 +0.1 2,729,231 - ABZR87_RS12830 0.73 -1.5 2,729,242 + ABZR87_RS12830 0.74 -0.9 2,729,242 + ABZR87_RS12830 0.74 +0.8 2,729,284 + ABZR87_RS12830 0.77 +0.7 2,729,347 + ABZR87_RS12830 0.82 -2.3 2,729,437 + ABZR87_RS12830 0.89 -0.3 2,729,437 + ABZR87_RS12830 0.89 -2.4 2,729,438 - ABZR87_RS12830 0.90 -0.2 2,729,438 - ABZR87_RS12830 0.90 +0.1 2,729,438 - ABZR87_RS12830 0.90 -0.7 2,729,470 + +0.8 2,729,495 - -0.3 2,729,495 - -0.1 2,729,551 + -2.4 2,729,551 + -0.5 2,729,552 - -0.6 2,729,552 - -0.3 2,729,552 - -0.9 2,729,552 - +0.2 2,729,552 - -1.1 2,729,552 - -0.8 2,729,713 - ABZR87_RS12835 0.10 -1.6 2,729,713 - ABZR87_RS12835 0.10 +1.1 2,729,713 - ABZR87_RS12835 0.10 -0.6 2,729,757 + ABZR87_RS12835 0.13 -0.2 2,729,820 + ABZR87_RS12835 0.17 +0.9 2,729,850 + ABZR87_RS12835 0.19 +1.5 2,729,850 + ABZR87_RS12835 0.19 -1.0 2,729,850 + ABZR87_RS12835 0.19 +0.3 2,729,851 - ABZR87_RS12835 0.19 +0.4 2,729,938 - ABZR87_RS12835 0.25 -1.2 2,729,938 - ABZR87_RS12835 0.25 -0.5 2,729,938 - ABZR87_RS12835 0.25 -0.0 2,729,976 + ABZR87_RS12835 0.28 -0.8 2,729,976 + ABZR87_RS12835 0.28 -1.4 2,729,976 + ABZR87_RS12835 0.28 +1.1 2,730,015 + ABZR87_RS12835 0.31 -2.8 2,730,015 + ABZR87_RS12835 0.31 -0.1 2,730,016 - ABZR87_RS12835 0.31 -0.9 2,730,016 - ABZR87_RS12835 0.31 +0.5 2,730,118 - ABZR87_RS12835 0.38 -1.2 2,730,118 - ABZR87_RS12835 0.38 +0.0 2,730,154 - ABZR87_RS12835 0.40 -1.5 2,730,154 - ABZR87_RS12835 0.40 +0.1 2,730,213 + ABZR87_RS12835 0.44 +0.4 2,730,214 - ABZR87_RS12835 0.44 -0.3 2,730,222 + ABZR87_RS12835 0.45 +0.9 2,730,223 - ABZR87_RS12835 0.45 -0.2 2,730,223 - ABZR87_RS12835 0.45 -0.4 2,730,306 + ABZR87_RS12835 0.50 +0.3 2,730,339 + ABZR87_RS12835 0.53 -0.5 2,730,340 - ABZR87_RS12835 0.53 +0.9 2,730,340 - ABZR87_RS12835 0.53 -0.0 2,730,340 - ABZR87_RS12835 0.53 +0.0 2,730,343 - ABZR87_RS12835 0.53 -0.0 2,730,420 + ABZR87_RS12835 0.58 +0.6 2,730,421 - ABZR87_RS12835 0.58 -2.4 2,730,421 - ABZR87_RS12835 0.58 -0.7 2,730,439 - ABZR87_RS12835 0.59 +0.1 2,730,439 - ABZR87_RS12835 0.59 -2.4 2,730,562 - ABZR87_RS12835 0.68 -1.0 2,730,562 - ABZR87_RS12835 0.68 -0.4 2,730,562 - ABZR87_RS12835 0.68 +0.2 2,730,562 - ABZR87_RS12835 0.68 -0.7 2,730,562 - ABZR87_RS12835 0.68 -0.2 2,730,562 - ABZR87_RS12835 0.68 -1.6
Or see this region's nucleotide sequence