Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS12500 and ABZR87_RS12505 are separated by 100 nucleotides ABZR87_RS12505 and ABZR87_RS12510 overlap by 11 nucleotides ABZR87_RS12510 and ABZR87_RS12515 are separated by 9 nucleotides ABZR87_RS12515 and ABZR87_RS12520 are separated by 23 nucleotides
ABZR87_RS12500: ABZR87_RS12500 - sterol desaturase family protein, at 2,647,041 to 2,647,943
_RS12500
ABZR87_RS12505: ABZR87_RS12505 - LysR family transcriptional regulator, at 2,648,044 to 2,648,949
_RS12505
ABZR87_RS12510: ABZR87_RS12510 - GNAT family N-acetyltransferase, at 2,648,939 to 2,649,391
_RS12510
ABZR87_RS12515: ABZR87_RS12515 - thioesterase family protein, at 2,649,401 to 2,649,838
_RS12515
ABZR87_RS12520: ABZR87_RS12520 - alpha/beta hydrolase, at 2,649,862 to 2,650,842
_RS12520
Position (kb)
2648
2649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2647.151 kb on - strand, within ABZR87_RS12500 at 2647.151 kb on - strand, within ABZR87_RS12500 at 2647.219 kb on + strand, within ABZR87_RS12500 at 2647.232 kb on + strand, within ABZR87_RS12500 at 2647.232 kb on + strand, within ABZR87_RS12500 at 2647.233 kb on - strand, within ABZR87_RS12500 at 2647.291 kb on + strand, within ABZR87_RS12500 at 2647.291 kb on + strand, within ABZR87_RS12500 at 2647.292 kb on - strand, within ABZR87_RS12500 at 2647.292 kb on - strand, within ABZR87_RS12500 at 2647.292 kb on - strand, within ABZR87_RS12500 at 2647.389 kb on + strand, within ABZR87_RS12500 at 2647.390 kb on - strand, within ABZR87_RS12500 at 2647.403 kb on - strand, within ABZR87_RS12500 at 2647.403 kb on - strand, within ABZR87_RS12500 at 2647.404 kb on + strand, within ABZR87_RS12500 at 2647.404 kb on + strand, within ABZR87_RS12500 at 2647.405 kb on - strand, within ABZR87_RS12500 at 2647.469 kb on - strand, within ABZR87_RS12500 at 2647.469 kb on - strand, within ABZR87_RS12500 at 2647.469 kb on - strand, within ABZR87_RS12500 at 2647.469 kb on - strand, within ABZR87_RS12500 at 2647.477 kb on + strand, within ABZR87_RS12500 at 2647.478 kb on - strand, within ABZR87_RS12500 at 2647.478 kb on - strand, within ABZR87_RS12500 at 2647.498 kb on + strand, within ABZR87_RS12500 at 2647.499 kb on - strand, within ABZR87_RS12500 at 2647.540 kb on + strand, within ABZR87_RS12500 at 2647.541 kb on - strand, within ABZR87_RS12500 at 2647.543 kb on + strand, within ABZR87_RS12500 at 2647.544 kb on - strand, within ABZR87_RS12500 at 2647.544 kb on - strand, within ABZR87_RS12500 at 2647.544 kb on - strand, within ABZR87_RS12500 at 2647.582 kb on + strand, within ABZR87_RS12500 at 2647.582 kb on + strand, within ABZR87_RS12500 at 2647.583 kb on - strand, within ABZR87_RS12500 at 2647.651 kb on + strand, within ABZR87_RS12500 at 2647.652 kb on - strand, within ABZR87_RS12500 at 2647.652 kb on - strand, within ABZR87_RS12500 at 2647.652 kb on - strand, within ABZR87_RS12500 at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.978 kb on + strand at 2647.979 kb on - strand at 2647.979 kb on - strand at 2648.030 kb on + strand at 2648.031 kb on - strand at 2648.296 kb on + strand, within ABZR87_RS12505 at 2648.416 kb on + strand, within ABZR87_RS12505 at 2648.516 kb on - strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.551 kb on + strand, within ABZR87_RS12505 at 2648.552 kb on - strand, within ABZR87_RS12505 at 2648.552 kb on - strand, within ABZR87_RS12505 at 2648.611 kb on + strand, within ABZR87_RS12505 at 2648.612 kb on - strand, within ABZR87_RS12505 at 2648.616 kb on + strand, within ABZR87_RS12505 at 2648.720 kb on - strand, within ABZR87_RS12505 at 2648.720 kb on - strand, within ABZR87_RS12505 at 2648.726 kb on + strand, within ABZR87_RS12505 at 2648.947 kb on + strand at 2648.947 kb on + strand at 2648.948 kb on - strand at 2649.011 kb on - strand, within ABZR87_RS12510 at 2649.011 kb on - strand, within ABZR87_RS12510 at 2649.027 kb on + strand, within ABZR87_RS12510 at 2649.027 kb on + strand, within ABZR87_RS12510 at 2649.028 kb on - strand, within ABZR87_RS12510 at 2649.028 kb on - strand, within ABZR87_RS12510 at 2649.028 kb on - strand, within ABZR87_RS12510 at 2649.303 kb on + strand, within ABZR87_RS12510 at 2649.304 kb on - strand, within ABZR87_RS12510 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.498 kb on + strand, within ABZR87_RS12515 at 2649.499 kb on - strand, within ABZR87_RS12515 at 2649.499 kb on - strand, within ABZR87_RS12515 at 2649.499 kb on - strand, within ABZR87_RS12515 at 2649.537 kb on + strand, within ABZR87_RS12515 at 2649.537 kb on + strand, within ABZR87_RS12515 at 2649.537 kb on + strand, within ABZR87_RS12515 at 2649.537 kb on + strand, within ABZR87_RS12515 at 2649.538 kb on - strand, within ABZR87_RS12515 at 2649.538 kb on - strand, within ABZR87_RS12515 at 2649.538 kb on - strand, within ABZR87_RS12515 at 2649.538 kb on - strand, within ABZR87_RS12515 at 2649.538 kb on - strand, within ABZR87_RS12515 at 2649.550 kb on + strand, within ABZR87_RS12515 at 2649.551 kb on - strand, within ABZR87_RS12515 at 2649.630 kb on + strand, within ABZR87_RS12515 at 2649.717 kb on + strand, within ABZR87_RS12515 at 2649.718 kb on - strand, within ABZR87_RS12515 at 2649.718 kb on - strand, within ABZR87_RS12515 at 2649.750 kb on + strand, within ABZR87_RS12515 at 2649.751 kb on - strand, within ABZR87_RS12515 at 2649.798 kb on - strand at 2649.798 kb on - strand at 2649.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 2,647,151 - ABZR87_RS12500 0.12 -0.9 2,647,151 - ABZR87_RS12500 0.12 +2.4 2,647,219 + ABZR87_RS12500 0.20 +0.3 2,647,232 + ABZR87_RS12500 0.21 +0.0 2,647,232 + ABZR87_RS12500 0.21 +0.7 2,647,233 - ABZR87_RS12500 0.21 -0.1 2,647,291 + ABZR87_RS12500 0.28 -0.2 2,647,291 + ABZR87_RS12500 0.28 +0.9 2,647,292 - ABZR87_RS12500 0.28 +1.1 2,647,292 - ABZR87_RS12500 0.28 +0.0 2,647,292 - ABZR87_RS12500 0.28 +0.5 2,647,389 + ABZR87_RS12500 0.39 -0.1 2,647,390 - ABZR87_RS12500 0.39 -2.8 2,647,403 - ABZR87_RS12500 0.40 -1.3 2,647,403 - ABZR87_RS12500 0.40 +0.2 2,647,404 + ABZR87_RS12500 0.40 +1.0 2,647,404 + ABZR87_RS12500 0.40 -1.8 2,647,405 - ABZR87_RS12500 0.40 +0.1 2,647,469 - ABZR87_RS12500 0.47 -0.1 2,647,469 - ABZR87_RS12500 0.47 +0.8 2,647,469 - ABZR87_RS12500 0.47 +0.1 2,647,469 - ABZR87_RS12500 0.47 -0.3 2,647,477 + ABZR87_RS12500 0.48 -2.1 2,647,478 - ABZR87_RS12500 0.48 -0.9 2,647,478 - ABZR87_RS12500 0.48 +0.5 2,647,498 + ABZR87_RS12500 0.51 +0.5 2,647,499 - ABZR87_RS12500 0.51 -0.2 2,647,540 + ABZR87_RS12500 0.55 +1.2 2,647,541 - ABZR87_RS12500 0.55 -0.6 2,647,543 + ABZR87_RS12500 0.56 +0.1 2,647,544 - ABZR87_RS12500 0.56 -0.4 2,647,544 - ABZR87_RS12500 0.56 +0.3 2,647,544 - ABZR87_RS12500 0.56 -0.3 2,647,582 + ABZR87_RS12500 0.60 -2.3 2,647,582 + ABZR87_RS12500 0.60 -1.1 2,647,583 - ABZR87_RS12500 0.60 -0.8 2,647,651 + ABZR87_RS12500 0.68 -0.7 2,647,652 - ABZR87_RS12500 0.68 -0.8 2,647,652 - ABZR87_RS12500 0.68 -0.4 2,647,652 - ABZR87_RS12500 0.68 -1.7 2,647,978 + -2.7 2,647,978 + -0.8 2,647,978 + -0.2 2,647,978 + -0.1 2,647,978 + -2.0 2,647,978 + -0.3 2,647,978 + +1.5 2,647,978 + +0.3 2,647,979 - +0.9 2,647,979 - +1.8 2,648,030 + -0.2 2,648,031 - -0.8 2,648,296 + ABZR87_RS12505 0.28 +2.6 2,648,416 + ABZR87_RS12505 0.41 +1.3 2,648,516 - ABZR87_RS12505 0.52 +0.1 2,648,551 + ABZR87_RS12505 0.56 -0.9 2,648,551 + ABZR87_RS12505 0.56 -0.8 2,648,551 + ABZR87_RS12505 0.56 +2.0 2,648,551 + ABZR87_RS12505 0.56 +0.9 2,648,551 + ABZR87_RS12505 0.56 +0.1 2,648,551 + ABZR87_RS12505 0.56 -1.7 2,648,552 - ABZR87_RS12505 0.56 +0.9 2,648,552 - ABZR87_RS12505 0.56 -2.0 2,648,611 + ABZR87_RS12505 0.63 -0.1 2,648,612 - ABZR87_RS12505 0.63 +1.5 2,648,616 + ABZR87_RS12505 0.63 -0.2 2,648,720 - ABZR87_RS12505 0.75 +0.9 2,648,720 - ABZR87_RS12505 0.75 -1.2 2,648,726 + ABZR87_RS12505 0.75 +1.4 2,648,947 + -2.2 2,648,947 + +1.1 2,648,948 - +1.1 2,649,011 - ABZR87_RS12510 0.16 -0.6 2,649,011 - ABZR87_RS12510 0.16 +0.5 2,649,027 + ABZR87_RS12510 0.19 -0.1 2,649,027 + ABZR87_RS12510 0.19 +0.1 2,649,028 - ABZR87_RS12510 0.20 -0.1 2,649,028 - ABZR87_RS12510 0.20 +0.3 2,649,028 - ABZR87_RS12510 0.20 -0.1 2,649,303 + ABZR87_RS12510 0.80 +3.1 2,649,304 - ABZR87_RS12510 0.81 -0.4 2,649,498 + ABZR87_RS12515 0.22 -1.4 2,649,498 + ABZR87_RS12515 0.22 +0.9 2,649,498 + ABZR87_RS12515 0.22 +0.5 2,649,498 + ABZR87_RS12515 0.22 -0.8 2,649,498 + ABZR87_RS12515 0.22 -1.1 2,649,498 + ABZR87_RS12515 0.22 +0.2 2,649,498 + ABZR87_RS12515 0.22 +0.0 2,649,498 + ABZR87_RS12515 0.22 -0.5 2,649,498 + ABZR87_RS12515 0.22 -0.0 2,649,499 - ABZR87_RS12515 0.22 +0.2 2,649,499 - ABZR87_RS12515 0.22 -0.0 2,649,499 - ABZR87_RS12515 0.22 +1.1 2,649,537 + ABZR87_RS12515 0.31 -0.9 2,649,537 + ABZR87_RS12515 0.31 -0.4 2,649,537 + ABZR87_RS12515 0.31 +1.2 2,649,537 + ABZR87_RS12515 0.31 +1.9 2,649,538 - ABZR87_RS12515 0.31 -0.2 2,649,538 - ABZR87_RS12515 0.31 +0.1 2,649,538 - ABZR87_RS12515 0.31 -3.2 2,649,538 - ABZR87_RS12515 0.31 -0.0 2,649,538 - ABZR87_RS12515 0.31 -1.3 2,649,550 + ABZR87_RS12515 0.34 +0.5 2,649,551 - ABZR87_RS12515 0.34 -0.7 2,649,630 + ABZR87_RS12515 0.52 +1.4 2,649,717 + ABZR87_RS12515 0.72 +0.8 2,649,718 - ABZR87_RS12515 0.72 -2.5 2,649,718 - ABZR87_RS12515 0.72 +1.4 2,649,750 + ABZR87_RS12515 0.80 +0.8 2,649,751 - ABZR87_RS12515 0.80 -0.6 2,649,798 - -0.3 2,649,798 - +1.9 2,649,798 - -0.4
Or see this region's nucleotide sequence