Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS12350

Experiment: root EcoFAB sample 2; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS12340 and ABZR87_RS12345 are separated by 1 nucleotidesABZR87_RS12345 and ABZR87_RS12350 are separated by 37 nucleotidesABZR87_RS12350 and ABZR87_RS12355 are separated by 165 nucleotidesABZR87_RS12355 and ABZR87_RS12360 overlap by 32 nucleotides ABZR87_RS12340: ABZR87_RS12340 - 8-amino-7-oxononanoate synthase, at 2,617,750 to 2,618,997 _RS12340 ABZR87_RS12345: ABZR87_RS12345 - dethiobiotin synthase, at 2,618,999 to 2,619,706 _RS12345 ABZR87_RS12350: ABZR87_RS12350 - hypothetical protein, at 2,619,744 to 2,620,262 _RS12350 ABZR87_RS12355: ABZR87_RS12355 - hypothetical protein, at 2,620,428 to 2,620,952 _RS12355 ABZR87_RS12360: ABZR87_RS12360 - DUF817 domain-containing protein, at 2,620,921 to 2,621,772 _RS12360 Position (kb) 2619 2620 2621Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 2618.919 kb on + strandat 2618.920 kb on - strandat 2618.920 kb on - strandat 2618.920 kb on - strandat 2619.395 kb on - strand, within ABZR87_RS12345at 2619.709 kb on + strandat 2619.710 kb on - strandat 2619.895 kb on - strand, within ABZR87_RS12350at 2619.895 kb on - strand, within ABZR87_RS12350at 2619.895 kb on - strand, within ABZR87_RS12350at 2619.895 kb on - strand, within ABZR87_RS12350at 2619.925 kb on - strand, within ABZR87_RS12350at 2619.939 kb on + strand, within ABZR87_RS12350at 2619.940 kb on - strand, within ABZR87_RS12350at 2619.940 kb on - strand, within ABZR87_RS12350at 2620.095 kb on + strand, within ABZR87_RS12350at 2620.095 kb on + strand, within ABZR87_RS12350at 2620.173 kb on + strand, within ABZR87_RS12350at 2620.227 kb on + strandat 2620.260 kb on + strandat 2620.260 kb on + strandat 2620.260 kb on + strandat 2620.340 kb on + strandat 2620.340 kb on + strandat 2620.340 kb on + strandat 2620.341 kb on - strandat 2620.341 kb on - strandat 2620.345 kb on + strandat 2620.345 kb on + strandat 2620.345 kb on + strandat 2620.346 kb on - strandat 2620.346 kb on - strandat 2620.346 kb on - strandat 2620.485 kb on + strand, within ABZR87_RS12355at 2620.485 kb on + strand, within ABZR87_RS12355at 2620.486 kb on - strand, within ABZR87_RS12355at 2620.650 kb on + strand, within ABZR87_RS12355at 2620.651 kb on - strand, within ABZR87_RS12355at 2620.651 kb on - strand, within ABZR87_RS12355at 2620.756 kb on + strand, within ABZR87_RS12355at 2620.756 kb on + strand, within ABZR87_RS12355at 2620.756 kb on + strand, within ABZR87_RS12355at 2620.757 kb on - strand, within ABZR87_RS12355at 2620.757 kb on - strand, within ABZR87_RS12355at 2620.757 kb on - strand, within ABZR87_RS12355at 2620.757 kb on - strand, within ABZR87_RS12355at 2620.888 kb on + strand, within ABZR87_RS12355at 2620.888 kb on + strand, within ABZR87_RS12355at 2620.889 kb on - strand, within ABZR87_RS12355at 2620.970 kb on + strandat 2620.970 kb on + strandat 2620.971 kb on - strandat 2620.971 kb on - strandat 2620.971 kb on - strandat 2620.971 kb on - strandat 2620.971 kb on - strandat 2620.971 kb on - strandat 2621.048 kb on + strand, within ABZR87_RS12360at 2621.112 kb on + strand, within ABZR87_RS12360at 2621.113 kb on - strand, within ABZR87_RS12360at 2621.113 kb on - strand, within ABZR87_RS12360at 2621.210 kb on + strand, within ABZR87_RS12360at 2621.210 kb on + strand, within ABZR87_RS12360at 2621.211 kb on - strand, within ABZR87_RS12360at 2621.228 kb on + strand, within ABZR87_RS12360at 2621.229 kb on - strand, within ABZR87_RS12360at 2621.229 kb on - strand, within ABZR87_RS12360at 2621.253 kb on - strand, within ABZR87_RS12360

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Per-strain Table

Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB
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2,618,919 + -2.8
2,618,920 - +0.0
2,618,920 - +1.8
2,618,920 - +1.6
2,619,395 - ABZR87_RS12345 0.56 -1.7
2,619,709 + +1.8
2,619,710 - +5.9
2,619,895 - ABZR87_RS12350 0.29 -2.3
2,619,895 - ABZR87_RS12350 0.29 -2.9
2,619,895 - ABZR87_RS12350 0.29 +1.4
2,619,895 - ABZR87_RS12350 0.29 +0.2
2,619,925 - ABZR87_RS12350 0.35 +0.5
2,619,939 + ABZR87_RS12350 0.38 +0.2
2,619,940 - ABZR87_RS12350 0.38 +0.3
2,619,940 - ABZR87_RS12350 0.38 +0.0
2,620,095 + ABZR87_RS12350 0.68 -0.1
2,620,095 + ABZR87_RS12350 0.68 -1.5
2,620,173 + ABZR87_RS12350 0.83 -1.4
2,620,227 + -2.1
2,620,260 + -0.1
2,620,260 + +0.5
2,620,260 + -0.2
2,620,340 + -1.3
2,620,340 + +2.1
2,620,340 + +0.6
2,620,341 - +1.7
2,620,341 - -0.1
2,620,345 + -1.6
2,620,345 + -0.0
2,620,345 + +1.1
2,620,346 - -3.0
2,620,346 - -0.4
2,620,346 - +0.7
2,620,485 + ABZR87_RS12355 0.11 -0.1
2,620,485 + ABZR87_RS12355 0.11 -1.1
2,620,486 - ABZR87_RS12355 0.11 +1.2
2,620,650 + ABZR87_RS12355 0.42 +1.3
2,620,651 - ABZR87_RS12355 0.42 -3.2
2,620,651 - ABZR87_RS12355 0.42 -2.7
2,620,756 + ABZR87_RS12355 0.62 -0.8
2,620,756 + ABZR87_RS12355 0.62 -0.4
2,620,756 + ABZR87_RS12355 0.62 -0.7
2,620,757 - ABZR87_RS12355 0.63 -1.7
2,620,757 - ABZR87_RS12355 0.63 -1.5
2,620,757 - ABZR87_RS12355 0.63 +3.3
2,620,757 - ABZR87_RS12355 0.63 +1.1
2,620,888 + ABZR87_RS12355 0.88 -0.7
2,620,888 + ABZR87_RS12355 0.88 +0.4
2,620,889 - ABZR87_RS12355 0.88 -1.3
2,620,970 + -0.1
2,620,970 + +0.7
2,620,971 - -0.1
2,620,971 - -0.5
2,620,971 - +0.5
2,620,971 - -1.0
2,620,971 - -1.2
2,620,971 - +0.2
2,621,048 + ABZR87_RS12360 0.15 -0.4
2,621,112 + ABZR87_RS12360 0.22 -1.5
2,621,113 - ABZR87_RS12360 0.23 +1.0
2,621,113 - ABZR87_RS12360 0.23 -0.2
2,621,210 + ABZR87_RS12360 0.34 -0.3
2,621,210 + ABZR87_RS12360 0.34 +1.6
2,621,211 - ABZR87_RS12360 0.34 +0.9
2,621,228 + ABZR87_RS12360 0.36 +0.7
2,621,229 - ABZR87_RS12360 0.36 -0.1
2,621,229 - ABZR87_RS12360 0.36 +0.1
2,621,253 - ABZR87_RS12360 0.39 -0.1

Or see this region's nucleotide sequence