Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10990 and ABZR87_RS10995 overlap by 1 nucleotides ABZR87_RS10995 and ABZR87_RS11000 are separated by 15 nucleotides ABZR87_RS11000 and ABZR87_RS11005 are separated by 149 nucleotides ABZR87_RS11005 and ABZR87_RS11010 are separated by 145 nucleotides
ABZR87_RS10990: ABZR87_RS10990 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 2,328,344 to 2,329,096
_RS10990
ABZR87_RS10995: ABZR87_RS10995 - NAD-dependent deacylase, at 2,329,096 to 2,329,845
_RS10995
ABZR87_RS11000: ABZR87_RS11000 - transaldolase, at 2,329,861 to 2,330,820
_RS11000
ABZR87_RS11005: ABZR87_RS11005 - ribose-5-phosphate isomerase RpiA, at 2,330,970 to 2,331,656
_RS11005
ABZR87_RS11010: ABZR87_RS11010 - oxidative damage protection protein, at 2,331,802 to 2,332,077
_RS11010
Position (kb)
2329
2330
2331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2328.893 kb on + strand, within ABZR87_RS10990 at 2329.044 kb on + strand at 2329.044 kb on + strand at 2329.044 kb on + strand at 2329.044 kb on + strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.045 kb on - strand at 2329.094 kb on + strand at 2329.095 kb on - strand at 2329.095 kb on - strand at 2329.095 kb on - strand at 2329.292 kb on - strand, within ABZR87_RS10995 at 2329.292 kb on - strand, within ABZR87_RS10995 at 2329.296 kb on + strand, within ABZR87_RS10995 at 2329.296 kb on + strand, within ABZR87_RS10995 at 2329.296 kb on + strand, within ABZR87_RS10995 at 2329.296 kb on + strand, within ABZR87_RS10995 at 2329.297 kb on - strand, within ABZR87_RS10995 at 2329.297 kb on - strand, within ABZR87_RS10995 at 2329.297 kb on - strand, within ABZR87_RS10995 at 2329.297 kb on - strand, within ABZR87_RS10995 at 2329.324 kb on + strand, within ABZR87_RS10995 at 2329.325 kb on - strand, within ABZR87_RS10995 at 2329.325 kb on - strand, within ABZR87_RS10995 at 2329.633 kb on + strand, within ABZR87_RS10995 at 2329.633 kb on + strand, within ABZR87_RS10995 at 2329.634 kb on - strand, within ABZR87_RS10995 at 2329.656 kb on + strand, within ABZR87_RS10995 at 2329.656 kb on + strand, within ABZR87_RS10995 at 2329.657 kb on - strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.697 kb on + strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.698 kb on - strand, within ABZR87_RS10995 at 2329.699 kb on + strand, within ABZR87_RS10995 at 2329.699 kb on + strand, within ABZR87_RS10995 at 2329.700 kb on - strand, within ABZR87_RS10995 at 2329.700 kb on - strand, within ABZR87_RS10995 at 2329.778 kb on + strand at 2329.779 kb on - strand at 2329.779 kb on - strand at 2329.863 kb on - strand at 2329.863 kb on - strand at 2330.013 kb on + strand, within ABZR87_RS11000 at 2330.192 kb on + strand, within ABZR87_RS11000 at 2330.192 kb on + strand, within ABZR87_RS11000 at 2330.192 kb on + strand, within ABZR87_RS11000 at 2330.193 kb on - strand, within ABZR87_RS11000 at 2330.195 kb on + strand, within ABZR87_RS11000 at 2330.196 kb on - strand, within ABZR87_RS11000 at 2330.196 kb on - strand, within ABZR87_RS11000 at 2330.196 kb on - strand, within ABZR87_RS11000 at 2330.196 kb on - strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.201 kb on + strand, within ABZR87_RS11000 at 2330.202 kb on - strand, within ABZR87_RS11000 at 2330.202 kb on - strand, within ABZR87_RS11000 at 2330.294 kb on + strand, within ABZR87_RS11000 at 2330.294 kb on + strand, within ABZR87_RS11000 at 2330.294 kb on + strand, within ABZR87_RS11000 at 2330.295 kb on - strand, within ABZR87_RS11000 at 2330.295 kb on - strand, within ABZR87_RS11000 at 2330.486 kb on + strand, within ABZR87_RS11000 at 2330.744 kb on + strand at 2330.833 kb on + strand at 2330.834 kb on - strand at 2330.879 kb on + strand at 2330.879 kb on + strand at 2330.921 kb on + strand at 2330.921 kb on + strand at 2330.932 kb on + strand at 2330.933 kb on - strand at 2330.933 kb on - strand at 2331.709 kb on + strand at 2331.710 kb on - strand at 2331.786 kb on + strand at 2331.787 kb on - strand at 2331.792 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 2,328,893 + ABZR87_RS10990 0.73 +0.4 2,329,044 + +0.1 2,329,044 + -1.8 2,329,044 + -1.0 2,329,044 + -2.9 2,329,045 - -0.2 2,329,045 - -1.3 2,329,045 - -0.1 2,329,045 - +1.0 2,329,045 - -0.4 2,329,045 - -1.8 2,329,045 - -2.1 2,329,094 + +0.0 2,329,095 - -0.2 2,329,095 - -1.0 2,329,095 - -1.1 2,329,292 - ABZR87_RS10995 0.26 -1.8 2,329,292 - ABZR87_RS10995 0.26 +1.1 2,329,296 + ABZR87_RS10995 0.27 -0.0 2,329,296 + ABZR87_RS10995 0.27 -0.7 2,329,296 + ABZR87_RS10995 0.27 +0.8 2,329,296 + ABZR87_RS10995 0.27 +2.4 2,329,297 - ABZR87_RS10995 0.27 +0.2 2,329,297 - ABZR87_RS10995 0.27 -1.7 2,329,297 - ABZR87_RS10995 0.27 -0.4 2,329,297 - ABZR87_RS10995 0.27 -1.2 2,329,324 + ABZR87_RS10995 0.30 -0.7 2,329,325 - ABZR87_RS10995 0.31 -1.8 2,329,325 - ABZR87_RS10995 0.31 -1.2 2,329,633 + ABZR87_RS10995 0.72 +1.0 2,329,633 + ABZR87_RS10995 0.72 +0.5 2,329,634 - ABZR87_RS10995 0.72 +0.3 2,329,656 + ABZR87_RS10995 0.75 +0.1 2,329,656 + ABZR87_RS10995 0.75 -1.3 2,329,657 - ABZR87_RS10995 0.75 +0.9 2,329,697 + ABZR87_RS10995 0.80 -0.4 2,329,697 + ABZR87_RS10995 0.80 +0.8 2,329,697 + ABZR87_RS10995 0.80 +0.5 2,329,697 + ABZR87_RS10995 0.80 +0.5 2,329,697 + ABZR87_RS10995 0.80 +0.7 2,329,697 + ABZR87_RS10995 0.80 +0.2 2,329,697 + ABZR87_RS10995 0.80 -0.6 2,329,697 + ABZR87_RS10995 0.80 -0.3 2,329,698 - ABZR87_RS10995 0.80 +1.2 2,329,698 - ABZR87_RS10995 0.80 +1.0 2,329,698 - ABZR87_RS10995 0.80 +0.3 2,329,698 - ABZR87_RS10995 0.80 -0.2 2,329,698 - ABZR87_RS10995 0.80 +0.2 2,329,698 - ABZR87_RS10995 0.80 +1.2 2,329,698 - ABZR87_RS10995 0.80 +0.3 2,329,698 - ABZR87_RS10995 0.80 -1.6 2,329,698 - ABZR87_RS10995 0.80 +0.7 2,329,698 - ABZR87_RS10995 0.80 +0.2 2,329,698 - ABZR87_RS10995 0.80 -0.2 2,329,699 + ABZR87_RS10995 0.80 -0.8 2,329,699 + ABZR87_RS10995 0.80 +0.5 2,329,700 - ABZR87_RS10995 0.81 -1.3 2,329,700 - ABZR87_RS10995 0.81 +1.4 2,329,778 + -1.0 2,329,779 - +0.3 2,329,779 - -1.4 2,329,863 - -2.2 2,329,863 - +1.1 2,330,013 + ABZR87_RS11000 0.16 -2.5 2,330,192 + ABZR87_RS11000 0.34 -0.1 2,330,192 + ABZR87_RS11000 0.34 -1.4 2,330,192 + ABZR87_RS11000 0.34 -2.2 2,330,193 - ABZR87_RS11000 0.35 +1.0 2,330,195 + ABZR87_RS11000 0.35 -0.4 2,330,196 - ABZR87_RS11000 0.35 -0.8 2,330,196 - ABZR87_RS11000 0.35 -0.8 2,330,196 - ABZR87_RS11000 0.35 -0.8 2,330,196 - ABZR87_RS11000 0.35 -2.3 2,330,201 + ABZR87_RS11000 0.35 +0.3 2,330,201 + ABZR87_RS11000 0.35 -1.2 2,330,201 + ABZR87_RS11000 0.35 -0.2 2,330,201 + ABZR87_RS11000 0.35 -2.1 2,330,201 + ABZR87_RS11000 0.35 -0.6 2,330,201 + ABZR87_RS11000 0.35 -0.1 2,330,202 - ABZR87_RS11000 0.36 -0.7 2,330,202 - ABZR87_RS11000 0.36 +1.2 2,330,294 + ABZR87_RS11000 0.45 -2.2 2,330,294 + ABZR87_RS11000 0.45 -3.2 2,330,294 + ABZR87_RS11000 0.45 +0.9 2,330,295 - ABZR87_RS11000 0.45 +0.1 2,330,295 - ABZR87_RS11000 0.45 -1.9 2,330,486 + ABZR87_RS11000 0.65 -0.6 2,330,744 + +1.0 2,330,833 + -0.8 2,330,834 - -1.2 2,330,879 + -0.4 2,330,879 + -0.1 2,330,921 + +0.3 2,330,921 + +2.4 2,330,932 + +1.0 2,330,933 - +0.6 2,330,933 - -0.9 2,331,709 + -0.4 2,331,710 - -0.1 2,331,786 + -2.4 2,331,787 - -2.1 2,331,792 + -0.6
Or see this region's nucleotide sequence