Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10875 and ABZR87_RS10880 are separated by 37 nucleotides ABZR87_RS10880 and ABZR87_RS10885 are separated by 160 nucleotides
ABZR87_RS10875: ABZR87_RS10875 - RNA polymerase sigma factor RpoS, at 2,302,881 to 2,304,017
_RS10875
ABZR87_RS10880: ABZR87_RS10880 - endonuclease/exonuclease/phosphatase family protein, at 2,304,055 to 2,304,918
_RS10880
ABZR87_RS10885: ABZR87_RS10885 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 2,305,079 to 2,306,431
_RS10885
Position (kb)
2304
2305 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2303.205 kb on + strand, within ABZR87_RS10875 at 2303.206 kb on - strand, within ABZR87_RS10875 at 2303.206 kb on - strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.457 kb on + strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.458 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.514 kb on - strand, within ABZR87_RS10875 at 2303.699 kb on + strand, within ABZR87_RS10875 at 2303.699 kb on + strand, within ABZR87_RS10875 at 2303.844 kb on + strand, within ABZR87_RS10875 at 2303.844 kb on + strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.845 kb on - strand, within ABZR87_RS10875 at 2303.848 kb on + strand, within ABZR87_RS10875 at 2303.849 kb on - strand, within ABZR87_RS10875 at 2303.849 kb on - strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.874 kb on + strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2303.875 kb on - strand, within ABZR87_RS10875 at 2304.000 kb on - strand at 2304.016 kb on - strand at 2304.176 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.265 kb on + strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.266 kb on - strand, within ABZR87_RS10880 at 2304.491 kb on + strand, within ABZR87_RS10880 at 2304.492 kb on - strand, within ABZR87_RS10880 at 2304.494 kb on + strand, within ABZR87_RS10880 at 2304.495 kb on - strand, within ABZR87_RS10880 at 2304.495 kb on - strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.570 kb on + strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.571 kb on - strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.572 kb on + strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.573 kb on - strand, within ABZR87_RS10880 at 2304.598 kb on + strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.599 kb on - strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.710 kb on + strand, within ABZR87_RS10880 at 2304.711 kb on - strand, within ABZR87_RS10880 at 2304.711 kb on - strand, within ABZR87_RS10880 at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.937 kb on + strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.938 kb on - strand at 2304.994 kb on + strand at 2304.995 kb on - strand at 2304.995 kb on - strand at 2304.995 kb on - strand at 2304.995 kb on - strand at 2305.052 kb on - strand at 2305.070 kb on - strand at 2305.070 kb on - strand at 2305.070 kb on - strand at 2305.214 kb on + strand at 2305.214 kb on + strand at 2305.214 kb on + strand at 2305.215 kb on - strand, within ABZR87_RS10885 at 2305.215 kb on - strand, within ABZR87_RS10885 at 2305.215 kb on - strand, within ABZR87_RS10885 at 2305.242 kb on - strand, within ABZR87_RS10885 at 2305.265 kb on - strand, within ABZR87_RS10885 at 2305.265 kb on - strand, within ABZR87_RS10885 at 2305.265 kb on - strand, within ABZR87_RS10885 at 2305.265 kb on - strand, within ABZR87_RS10885 at 2305.460 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.544 kb on + strand, within ABZR87_RS10885 at 2305.545 kb on - strand, within ABZR87_RS10885 at 2305.545 kb on - strand, within ABZR87_RS10885 at 2305.545 kb on - strand, within ABZR87_RS10885 at 2305.545 kb on - strand, within ABZR87_RS10885 at 2305.587 kb on + strand, within ABZR87_RS10885 at 2305.587 kb on + strand, within ABZR87_RS10885 at 2305.587 kb on + strand, within ABZR87_RS10885 at 2305.588 kb on - strand, within ABZR87_RS10885 at 2305.588 kb on - strand, within ABZR87_RS10885 at 2305.645 kb on + strand, within ABZR87_RS10885 at 2305.645 kb on + strand, within ABZR87_RS10885 at 2305.645 kb on + strand, within ABZR87_RS10885 at 2305.645 kb on + strand, within ABZR87_RS10885 at 2305.645 kb on + strand, within ABZR87_RS10885 at 2305.646 kb on - strand, within ABZR87_RS10885 at 2305.646 kb on - strand, within ABZR87_RS10885 at 2305.693 kb on - strand, within ABZR87_RS10885 at 2305.693 kb on - strand, within ABZR87_RS10885 at 2305.803 kb on - strand, within ABZR87_RS10885 at 2305.803 kb on - strand, within ABZR87_RS10885 at 2305.835 kb on + strand, within ABZR87_RS10885 at 2305.835 kb on + strand, within ABZR87_RS10885 at 2305.836 kb on - strand, within ABZR87_RS10885 at 2305.836 kb on - strand, within ABZR87_RS10885 at 2305.836 kb on - strand, within ABZR87_RS10885
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 2,303,205 + ABZR87_RS10875 0.28 +1.9 2,303,206 - ABZR87_RS10875 0.29 +1.8 2,303,206 - ABZR87_RS10875 0.29 +1.0 2,303,457 + ABZR87_RS10875 0.51 +2.6 2,303,457 + ABZR87_RS10875 0.51 +1.7 2,303,457 + ABZR87_RS10875 0.51 +1.5 2,303,457 + ABZR87_RS10875 0.51 +1.8 2,303,457 + ABZR87_RS10875 0.51 +1.4 2,303,457 + ABZR87_RS10875 0.51 +1.8 2,303,457 + ABZR87_RS10875 0.51 +1.4 2,303,457 + ABZR87_RS10875 0.51 +2.8 2,303,458 - ABZR87_RS10875 0.51 +2.4 2,303,458 - ABZR87_RS10875 0.51 +1.6 2,303,458 - ABZR87_RS10875 0.51 +1.5 2,303,458 - ABZR87_RS10875 0.51 +3.8 2,303,458 - ABZR87_RS10875 0.51 +2.7 2,303,458 - ABZR87_RS10875 0.51 +1.9 2,303,458 - ABZR87_RS10875 0.51 +3.0 2,303,458 - ABZR87_RS10875 0.51 +1.4 2,303,458 - ABZR87_RS10875 0.51 +1.6 2,303,458 - ABZR87_RS10875 0.51 -0.8 2,303,514 - ABZR87_RS10875 0.56 +1.0 2,303,514 - ABZR87_RS10875 0.56 -0.3 2,303,514 - ABZR87_RS10875 0.56 +1.5 2,303,699 + ABZR87_RS10875 0.72 +3.7 2,303,699 + ABZR87_RS10875 0.72 +2.0 2,303,844 + ABZR87_RS10875 0.85 +1.8 2,303,844 + ABZR87_RS10875 0.85 +1.6 2,303,845 - ABZR87_RS10875 0.85 +1.6 2,303,845 - ABZR87_RS10875 0.85 +3.0 2,303,845 - ABZR87_RS10875 0.85 +1.1 2,303,845 - ABZR87_RS10875 0.85 +1.6 2,303,848 + ABZR87_RS10875 0.85 +1.4 2,303,849 - ABZR87_RS10875 0.85 +2.6 2,303,849 - ABZR87_RS10875 0.85 +2.5 2,303,874 + ABZR87_RS10875 0.87 +1.4 2,303,874 + ABZR87_RS10875 0.87 +1.5 2,303,874 + ABZR87_RS10875 0.87 +3.0 2,303,874 + ABZR87_RS10875 0.87 +1.4 2,303,875 - ABZR87_RS10875 0.87 +1.5 2,303,875 - ABZR87_RS10875 0.87 +1.1 2,303,875 - ABZR87_RS10875 0.87 +1.5 2,303,875 - ABZR87_RS10875 0.87 +2.3 2,303,875 - ABZR87_RS10875 0.87 +1.9 2,303,875 - ABZR87_RS10875 0.87 +3.5 2,303,875 - ABZR87_RS10875 0.87 +1.7 2,303,875 - ABZR87_RS10875 0.87 +1.8 2,304,000 - +1.2 2,304,016 - +0.7 2,304,176 + ABZR87_RS10880 0.14 +0.2 2,304,265 + ABZR87_RS10880 0.24 +0.4 2,304,265 + ABZR87_RS10880 0.24 -0.7 2,304,265 + ABZR87_RS10880 0.24 +2.5 2,304,265 + ABZR87_RS10880 0.24 -1.3 2,304,266 - ABZR87_RS10880 0.24 -0.8 2,304,266 - ABZR87_RS10880 0.24 +3.8 2,304,266 - ABZR87_RS10880 0.24 -1.4 2,304,491 + ABZR87_RS10880 0.50 -2.1 2,304,492 - ABZR87_RS10880 0.51 +0.2 2,304,494 + ABZR87_RS10880 0.51 -0.1 2,304,495 - ABZR87_RS10880 0.51 +0.7 2,304,495 - ABZR87_RS10880 0.51 +0.9 2,304,570 + ABZR87_RS10880 0.60 -1.0 2,304,570 + ABZR87_RS10880 0.60 +0.2 2,304,570 + ABZR87_RS10880 0.60 +0.1 2,304,570 + ABZR87_RS10880 0.60 -1.1 2,304,571 - ABZR87_RS10880 0.60 -0.3 2,304,571 - ABZR87_RS10880 0.60 -1.8 2,304,571 - ABZR87_RS10880 0.60 +1.3 2,304,572 + ABZR87_RS10880 0.60 +2.5 2,304,572 + ABZR87_RS10880 0.60 +0.5 2,304,572 + ABZR87_RS10880 0.60 +0.5 2,304,572 + ABZR87_RS10880 0.60 -1.4 2,304,572 + ABZR87_RS10880 0.60 -2.2 2,304,572 + ABZR87_RS10880 0.60 +0.2 2,304,572 + ABZR87_RS10880 0.60 -0.5 2,304,572 + ABZR87_RS10880 0.60 +1.1 2,304,572 + ABZR87_RS10880 0.60 -0.4 2,304,572 + ABZR87_RS10880 0.60 -0.7 2,304,572 + ABZR87_RS10880 0.60 -1.4 2,304,572 + ABZR87_RS10880 0.60 +0.3 2,304,572 + ABZR87_RS10880 0.60 -2.3 2,304,572 + ABZR87_RS10880 0.60 +0.6 2,304,573 - ABZR87_RS10880 0.60 -1.0 2,304,573 - ABZR87_RS10880 0.60 -1.0 2,304,573 - ABZR87_RS10880 0.60 -0.5 2,304,573 - ABZR87_RS10880 0.60 +0.5 2,304,573 - ABZR87_RS10880 0.60 -0.2 2,304,573 - ABZR87_RS10880 0.60 +0.0 2,304,573 - ABZR87_RS10880 0.60 -0.4 2,304,573 - ABZR87_RS10880 0.60 -1.0 2,304,573 - ABZR87_RS10880 0.60 +0.4 2,304,573 - ABZR87_RS10880 0.60 -0.1 2,304,573 - ABZR87_RS10880 0.60 +0.1 2,304,573 - ABZR87_RS10880 0.60 +0.9 2,304,573 - ABZR87_RS10880 0.60 -0.6 2,304,573 - ABZR87_RS10880 0.60 -0.1 2,304,573 - ABZR87_RS10880 0.60 -0.9 2,304,573 - ABZR87_RS10880 0.60 -0.2 2,304,573 - ABZR87_RS10880 0.60 +0.4 2,304,573 - ABZR87_RS10880 0.60 -0.3 2,304,598 + ABZR87_RS10880 0.63 -0.1 2,304,599 - ABZR87_RS10880 0.63 +0.7 2,304,599 - ABZR87_RS10880 0.63 -0.3 2,304,599 - ABZR87_RS10880 0.63 +0.2 2,304,599 - ABZR87_RS10880 0.63 -0.1 2,304,710 + ABZR87_RS10880 0.76 +0.2 2,304,710 + ABZR87_RS10880 0.76 -0.8 2,304,710 + ABZR87_RS10880 0.76 +0.2 2,304,711 - ABZR87_RS10880 0.76 -0.9 2,304,711 - ABZR87_RS10880 0.76 -2.6 2,304,937 + +0.5 2,304,937 + -0.2 2,304,937 + -0.4 2,304,937 + -1.7 2,304,937 + -0.0 2,304,937 + -2.7 2,304,937 + +0.6 2,304,937 + -0.5 2,304,937 + +1.2 2,304,938 - -0.2 2,304,938 - +0.3 2,304,938 - +0.5 2,304,938 - +1.3 2,304,938 - -0.9 2,304,938 - -1.1 2,304,994 + -2.0 2,304,995 - +0.0 2,304,995 - +2.3 2,304,995 - -1.0 2,304,995 - +0.2 2,305,052 - -0.7 2,305,070 - -0.3 2,305,070 - +1.3 2,305,070 - +1.6 2,305,214 + -1.6 2,305,214 + +0.6 2,305,214 + -0.7 2,305,215 - ABZR87_RS10885 0.10 -0.2 2,305,215 - ABZR87_RS10885 0.10 +0.2 2,305,215 - ABZR87_RS10885 0.10 -0.2 2,305,242 - ABZR87_RS10885 0.12 -0.4 2,305,265 - ABZR87_RS10885 0.14 +0.2 2,305,265 - ABZR87_RS10885 0.14 +4.0 2,305,265 - ABZR87_RS10885 0.14 -0.3 2,305,265 - ABZR87_RS10885 0.14 +0.4 2,305,460 + ABZR87_RS10885 0.28 -1.6 2,305,544 + ABZR87_RS10885 0.34 -1.3 2,305,544 + ABZR87_RS10885 0.34 -0.6 2,305,544 + ABZR87_RS10885 0.34 -0.9 2,305,544 + ABZR87_RS10885 0.34 -2.6 2,305,544 + ABZR87_RS10885 0.34 -0.6 2,305,544 + ABZR87_RS10885 0.34 -1.5 2,305,544 + ABZR87_RS10885 0.34 +1.2 2,305,544 + ABZR87_RS10885 0.34 +0.6 2,305,544 + ABZR87_RS10885 0.34 -2.0 2,305,545 - ABZR87_RS10885 0.34 -1.9 2,305,545 - ABZR87_RS10885 0.34 +1.1 2,305,545 - ABZR87_RS10885 0.34 -0.2 2,305,545 - ABZR87_RS10885 0.34 -0.6 2,305,587 + ABZR87_RS10885 0.38 +0.4 2,305,587 + ABZR87_RS10885 0.38 -0.7 2,305,587 + ABZR87_RS10885 0.38 -1.1 2,305,588 - ABZR87_RS10885 0.38 +0.4 2,305,588 - ABZR87_RS10885 0.38 -2.1 2,305,645 + ABZR87_RS10885 0.42 +0.2 2,305,645 + ABZR87_RS10885 0.42 -0.6 2,305,645 + ABZR87_RS10885 0.42 +0.3 2,305,645 + ABZR87_RS10885 0.42 +0.4 2,305,645 + ABZR87_RS10885 0.42 -1.6 2,305,646 - ABZR87_RS10885 0.42 +0.8 2,305,646 - ABZR87_RS10885 0.42 -0.1 2,305,693 - ABZR87_RS10885 0.45 -0.3 2,305,693 - ABZR87_RS10885 0.45 -0.4 2,305,803 - ABZR87_RS10885 0.54 -0.5 2,305,803 - ABZR87_RS10885 0.54 -2.2 2,305,835 + ABZR87_RS10885 0.56 -0.4 2,305,835 + ABZR87_RS10885 0.56 -1.1 2,305,836 - ABZR87_RS10885 0.56 +1.3 2,305,836 - ABZR87_RS10885 0.56 -1.6 2,305,836 - ABZR87_RS10885 0.56 +0.7
Or see this region's nucleotide sequence