Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10210 and ABZR87_RS10215 are separated by 32 nucleotides ABZR87_RS10215 and ABZR87_RS10220 overlap by 4 nucleotides ABZR87_RS10220 and ABZR87_RS10225 overlap by 4 nucleotides ABZR87_RS10225 and ABZR87_RS10230 overlap by 4 nucleotides
ABZR87_RS10210: ABZR87_RS10210 - GTPase Era, at 2,165,882 to 2,166,820
_RS10210
ABZR87_RS10215: ABZR87_RS10215 - DNA repair protein RecO, at 2,166,853 to 2,167,674
_RS10215
ABZR87_RS10220: ABZR87_RS10220 - pyridoxine 5'-phosphate synthase, at 2,167,671 to 2,168,462
_RS10220
ABZR87_RS10225: ABZR87_RS10225 - holo-ACP synthase, at 2,168,459 to 2,168,902
_RS10225
ABZR87_RS10230: ABZR87_RS10230 - beta-N-acetylhexosaminidase, at 2,168,899 to 2,169,948
_RS10230
Position (kb)
2167
2168
2169 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2166.806 kb on + strand at 2166.818 kb on + strand at 2166.819 kb on - strand at 2166.827 kb on - strand at 2166.841 kb on + strand at 2167.000 kb on + strand, within ABZR87_RS10215 at 2167.001 kb on - strand, within ABZR87_RS10215 at 2167.049 kb on - strand, within ABZR87_RS10215 at 2167.056 kb on + strand, within ABZR87_RS10215 at 2167.057 kb on - strand, within ABZR87_RS10215 at 2167.234 kb on + strand, within ABZR87_RS10215 at 2167.300 kb on + strand, within ABZR87_RS10215 at 2167.301 kb on - strand, within ABZR87_RS10215 at 2167.317 kb on + strand, within ABZR87_RS10215 at 2167.317 kb on + strand, within ABZR87_RS10215 at 2167.318 kb on - strand, within ABZR87_RS10215 at 2167.447 kb on + strand, within ABZR87_RS10215 at 2167.447 kb on + strand, within ABZR87_RS10215 at 2167.448 kb on - strand, within ABZR87_RS10215 at 2167.448 kb on - strand, within ABZR87_RS10215 at 2167.454 kb on - strand, within ABZR87_RS10215 at 2167.454 kb on - strand, within ABZR87_RS10215 at 2167.511 kb on + strand, within ABZR87_RS10215 at 2167.511 kb on + strand, within ABZR87_RS10215 at 2167.511 kb on + strand, within ABZR87_RS10215 at 2167.512 kb on - strand, within ABZR87_RS10215 at 2167.512 kb on - strand, within ABZR87_RS10215 at 2167.512 kb on - strand, within ABZR87_RS10215 at 2167.553 kb on - strand, within ABZR87_RS10215 at 2167.612 kb on + strand at 2167.612 kb on + strand at 2167.612 kb on + strand at 2167.613 kb on - strand at 2167.613 kb on - strand at 2167.670 kb on + strand at 2167.670 kb on + strand at 2167.670 kb on + strand at 2167.670 kb on + strand at 2167.670 kb on + strand at 2167.670 kb on + strand at 2168.458 kb on + strand at 2169.150 kb on + strand, within ABZR87_RS10230 at 2169.150 kb on + strand, within ABZR87_RS10230 at 2169.151 kb on - strand, within ABZR87_RS10230 at 2169.236 kb on - strand, within ABZR87_RS10230 at 2169.443 kb on - strand, within ABZR87_RS10230 at 2169.443 kb on - strand, within ABZR87_RS10230
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 2,166,806 + +1.8 2,166,818 + +0.8 2,166,819 - +0.8 2,166,827 - -0.5 2,166,841 + -0.6 2,167,000 + ABZR87_RS10215 0.18 -0.3 2,167,001 - ABZR87_RS10215 0.18 +0.0 2,167,049 - ABZR87_RS10215 0.24 -0.2 2,167,056 + ABZR87_RS10215 0.25 -0.8 2,167,057 - ABZR87_RS10215 0.25 -2.3 2,167,234 + ABZR87_RS10215 0.46 +0.4 2,167,300 + ABZR87_RS10215 0.54 +0.2 2,167,301 - ABZR87_RS10215 0.55 +0.4 2,167,317 + ABZR87_RS10215 0.56 -1.0 2,167,317 + ABZR87_RS10215 0.56 -1.0 2,167,318 - ABZR87_RS10215 0.57 +0.5 2,167,447 + ABZR87_RS10215 0.72 +0.1 2,167,447 + ABZR87_RS10215 0.72 -1.4 2,167,448 - ABZR87_RS10215 0.72 -0.5 2,167,448 - ABZR87_RS10215 0.72 +2.2 2,167,454 - ABZR87_RS10215 0.73 -1.7 2,167,454 - ABZR87_RS10215 0.73 +0.1 2,167,511 + ABZR87_RS10215 0.80 +2.6 2,167,511 + ABZR87_RS10215 0.80 -1.0 2,167,511 + ABZR87_RS10215 0.80 +1.1 2,167,512 - ABZR87_RS10215 0.80 -0.4 2,167,512 - ABZR87_RS10215 0.80 +0.1 2,167,512 - ABZR87_RS10215 0.80 -0.1 2,167,553 - ABZR87_RS10215 0.85 +0.5 2,167,612 + +0.6 2,167,612 + +0.0 2,167,612 + -1.1 2,167,613 - -1.9 2,167,613 - -0.1 2,167,670 + +0.6 2,167,670 + -0.6 2,167,670 + +0.1 2,167,670 + -1.4 2,167,670 + +0.3 2,167,670 + -0.4 2,168,458 + -0.2 2,169,150 + ABZR87_RS10230 0.24 +0.0 2,169,150 + ABZR87_RS10230 0.24 -0.1 2,169,151 - ABZR87_RS10230 0.24 -1.9 2,169,236 - ABZR87_RS10230 0.32 +0.9 2,169,443 - ABZR87_RS10230 0.52 -1.4 2,169,443 - ABZR87_RS10230 0.52 -1.2
Or see this region's nucleotide sequence