Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS08970 and ABZR87_RS08975 are separated by 12 nucleotides ABZR87_RS08975 and ABZR87_RS08980 are separated by 37 nucleotides ABZR87_RS08980 and ABZR87_RS08985 are separated by 66 nucleotides
ABZR87_RS08970: ABZR87_RS08970 - protein TolQ, at 1,904,073 to 1,904,765
_RS08970
ABZR87_RS08975: ABZR87_RS08975 - protein TolR, at 1,904,778 to 1,905,212
_RS08975
ABZR87_RS08980: ABZR87_RS08980 - cell envelope integrity protein TolA, at 1,905,250 to 1,906,278
_RS08980
ABZR87_RS08985: ABZR87_RS08985 - Tol-Pal system beta propeller repeat protein TolB, at 1,906,345 to 1,907,649
_RS08985
Position (kb)
1905
1906
1907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1904.287 kb on - strand, within ABZR87_RS08970 at 1904.287 kb on - strand, within ABZR87_RS08970 at 1904.429 kb on + strand, within ABZR87_RS08970 at 1904.429 kb on + strand, within ABZR87_RS08970 at 1904.429 kb on + strand, within ABZR87_RS08970 at 1904.430 kb on - strand, within ABZR87_RS08970 at 1904.430 kb on - strand, within ABZR87_RS08970 at 1904.430 kb on - strand, within ABZR87_RS08970 at 1904.430 kb on - strand, within ABZR87_RS08970 at 1904.442 kb on + strand, within ABZR87_RS08970 at 1904.442 kb on + strand, within ABZR87_RS08970 at 1904.443 kb on - strand, within ABZR87_RS08970 at 1904.511 kb on + strand, within ABZR87_RS08970 at 1904.511 kb on + strand, within ABZR87_RS08970 at 1904.511 kb on + strand, within ABZR87_RS08970 at 1904.512 kb on - strand, within ABZR87_RS08970 at 1904.512 kb on - strand, within ABZR87_RS08970 at 1904.512 kb on - strand, within ABZR87_RS08970 at 1904.670 kb on + strand, within ABZR87_RS08970 at 1904.671 kb on - strand, within ABZR87_RS08970 at 1904.671 kb on - strand, within ABZR87_RS08970 at 1904.671 kb on - strand, within ABZR87_RS08970 at 1904.680 kb on - strand, within ABZR87_RS08970 at 1904.783 kb on + strand at 1905.133 kb on - strand, within ABZR87_RS08975 at 1905.256 kb on - strand at 1905.256 kb on - strand at 1905.274 kb on + strand at 1905.274 kb on + strand at 1905.275 kb on - strand at 1905.867 kb on + strand, within ABZR87_RS08980 at 1905.868 kb on - strand, within ABZR87_RS08980 at 1905.868 kb on - strand, within ABZR87_RS08980 at 1906.171 kb on + strand, within ABZR87_RS08980 at 1906.171 kb on + strand, within ABZR87_RS08980 at 1906.172 kb on - strand, within ABZR87_RS08980 at 1906.172 kb on - strand, within ABZR87_RS08980 at 1906.172 kb on - strand, within ABZR87_RS08980 at 1906.172 kb on - strand, within ABZR87_RS08980 at 1906.224 kb on + strand at 1906.276 kb on + strand at 1906.277 kb on - strand at 1906.314 kb on + strand at 1906.334 kb on + strand at 1906.337 kb on + strand at 1906.337 kb on + strand at 1906.337 kb on + strand at 1906.337 kb on + strand at 1906.338 kb on - strand at 1906.338 kb on - strand at 1906.338 kb on - strand at 1906.338 kb on - strand at 1906.478 kb on - strand, within ABZR87_RS08985 at 1906.714 kb on + strand, within ABZR87_RS08985 at 1906.714 kb on + strand, within ABZR87_RS08985 at 1906.715 kb on - strand, within ABZR87_RS08985 at 1906.715 kb on - strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.804 kb on + strand, within ABZR87_RS08985 at 1906.805 kb on - strand, within ABZR87_RS08985 at 1906.805 kb on - strand, within ABZR87_RS08985 at 1906.805 kb on - strand, within ABZR87_RS08985 at 1906.810 kb on + strand, within ABZR87_RS08985 at 1906.811 kb on - strand, within ABZR87_RS08985 at 1906.811 kb on - strand, within ABZR87_RS08985 at 1906.858 kb on + strand, within ABZR87_RS08985 at 1906.858 kb on + strand, within ABZR87_RS08985 at 1906.859 kb on - strand, within ABZR87_RS08985 at 1906.859 kb on - strand, within ABZR87_RS08985 at 1906.859 kb on - strand, within ABZR87_RS08985 at 1906.990 kb on + strand, within ABZR87_RS08985 at 1906.990 kb on + strand, within ABZR87_RS08985 at 1906.990 kb on + strand, within ABZR87_RS08985 at 1906.990 kb on + strand, within ABZR87_RS08985 at 1906.990 kb on + strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1906.991 kb on - strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.023 kb on + strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.024 kb on - strand, within ABZR87_RS08985 at 1907.145 kb on + strand, within ABZR87_RS08985 at 1907.146 kb on - strand, within ABZR87_RS08985 at 1907.146 kb on - strand, within ABZR87_RS08985 at 1907.146 kb on - strand, within ABZR87_RS08985 at 1907.155 kb on + strand, within ABZR87_RS08985 at 1907.155 kb on + strand, within ABZR87_RS08985 at 1907.155 kb on + strand, within ABZR87_RS08985 at 1907.156 kb on - strand, within ABZR87_RS08985 at 1907.245 kb on + strand, within ABZR87_RS08985 at 1907.245 kb on + strand, within ABZR87_RS08985 at 1907.245 kb on + strand, within ABZR87_RS08985 at 1907.245 kb on + strand, within ABZR87_RS08985 at 1907.246 kb on - strand, within ABZR87_RS08985 at 1907.246 kb on - strand, within ABZR87_RS08985 at 1907.246 kb on - strand, within ABZR87_RS08985 at 1907.247 kb on + strand, within ABZR87_RS08985 at 1907.247 kb on + strand, within ABZR87_RS08985 at 1907.247 kb on + strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.248 kb on - strand, within ABZR87_RS08985 at 1907.251 kb on + strand, within ABZR87_RS08985 at 1907.251 kb on + strand, within ABZR87_RS08985 at 1907.252 kb on - strand, within ABZR87_RS08985 at 1907.252 kb on - strand, within ABZR87_RS08985
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 1,904,287 - ABZR87_RS08970 0.31 -0.4 1,904,287 - ABZR87_RS08970 0.31 -1.4 1,904,429 + ABZR87_RS08970 0.51 +1.0 1,904,429 + ABZR87_RS08970 0.51 -2.2 1,904,429 + ABZR87_RS08970 0.51 -2.0 1,904,430 - ABZR87_RS08970 0.52 -2.2 1,904,430 - ABZR87_RS08970 0.52 -0.3 1,904,430 - ABZR87_RS08970 0.52 -3.1 1,904,430 - ABZR87_RS08970 0.52 -0.9 1,904,442 + ABZR87_RS08970 0.53 -2.4 1,904,442 + ABZR87_RS08970 0.53 -1.5 1,904,443 - ABZR87_RS08970 0.53 -0.3 1,904,511 + ABZR87_RS08970 0.63 +0.5 1,904,511 + ABZR87_RS08970 0.63 -1.4 1,904,511 + ABZR87_RS08970 0.63 -0.0 1,904,512 - ABZR87_RS08970 0.63 +0.1 1,904,512 - ABZR87_RS08970 0.63 -1.8 1,904,512 - ABZR87_RS08970 0.63 -1.2 1,904,670 + ABZR87_RS08970 0.86 -1.7 1,904,671 - ABZR87_RS08970 0.86 -1.8 1,904,671 - ABZR87_RS08970 0.86 +0.2 1,904,671 - ABZR87_RS08970 0.86 -0.9 1,904,680 - ABZR87_RS08970 0.88 -1.3 1,904,783 + -1.7 1,905,133 - ABZR87_RS08975 0.82 -1.4 1,905,256 - -0.2 1,905,256 - -1.2 1,905,274 + +1.2 1,905,274 + -2.0 1,905,275 - +0.3 1,905,867 + ABZR87_RS08980 0.60 -0.2 1,905,868 - ABZR87_RS08980 0.60 +0.3 1,905,868 - ABZR87_RS08980 0.60 +0.2 1,906,171 + ABZR87_RS08980 0.90 -1.4 1,906,171 + ABZR87_RS08980 0.90 -3.5 1,906,172 - ABZR87_RS08980 0.90 -0.5 1,906,172 - ABZR87_RS08980 0.90 -1.4 1,906,172 - ABZR87_RS08980 0.90 -0.0 1,906,172 - ABZR87_RS08980 0.90 -0.9 1,906,224 + -3.0 1,906,276 + +0.0 1,906,277 - -1.2 1,906,314 + -1.4 1,906,334 + +1.5 1,906,337 + -0.8 1,906,337 + +3.9 1,906,337 + -0.5 1,906,337 + -1.7 1,906,338 - +0.6 1,906,338 - +0.2 1,906,338 - -0.8 1,906,338 - -3.2 1,906,478 - ABZR87_RS08985 0.10 +1.2 1,906,714 + ABZR87_RS08985 0.28 -0.8 1,906,714 + ABZR87_RS08985 0.28 -0.3 1,906,715 - ABZR87_RS08985 0.28 +0.9 1,906,715 - ABZR87_RS08985 0.28 -2.3 1,906,804 + ABZR87_RS08985 0.35 -2.7 1,906,804 + ABZR87_RS08985 0.35 -1.7 1,906,804 + ABZR87_RS08985 0.35 -0.1 1,906,804 + ABZR87_RS08985 0.35 -0.5 1,906,804 + ABZR87_RS08985 0.35 -0.6 1,906,804 + ABZR87_RS08985 0.35 -0.8 1,906,805 - ABZR87_RS08985 0.35 -1.2 1,906,805 - ABZR87_RS08985 0.35 +0.9 1,906,805 - ABZR87_RS08985 0.35 -1.1 1,906,810 + ABZR87_RS08985 0.36 -2.4 1,906,811 - ABZR87_RS08985 0.36 -1.1 1,906,811 - ABZR87_RS08985 0.36 -0.1 1,906,858 + ABZR87_RS08985 0.39 +0.3 1,906,858 + ABZR87_RS08985 0.39 -1.5 1,906,859 - ABZR87_RS08985 0.39 +0.0 1,906,859 - ABZR87_RS08985 0.39 -2.5 1,906,859 - ABZR87_RS08985 0.39 -0.2 1,906,990 + ABZR87_RS08985 0.49 -0.8 1,906,990 + ABZR87_RS08985 0.49 -2.4 1,906,990 + ABZR87_RS08985 0.49 -0.7 1,906,990 + ABZR87_RS08985 0.49 -0.5 1,906,990 + ABZR87_RS08985 0.49 -1.5 1,906,991 - ABZR87_RS08985 0.50 -0.2 1,906,991 - ABZR87_RS08985 0.50 -0.3 1,906,991 - ABZR87_RS08985 0.50 -0.8 1,906,991 - ABZR87_RS08985 0.50 -1.9 1,906,991 - ABZR87_RS08985 0.50 +0.6 1,906,991 - ABZR87_RS08985 0.50 -1.7 1,906,991 - ABZR87_RS08985 0.50 -0.2 1,906,991 - ABZR87_RS08985 0.50 -0.8 1,907,023 + ABZR87_RS08985 0.52 -0.7 1,907,023 + ABZR87_RS08985 0.52 -1.0 1,907,023 + ABZR87_RS08985 0.52 -0.7 1,907,023 + ABZR87_RS08985 0.52 -0.4 1,907,023 + ABZR87_RS08985 0.52 -1.6 1,907,023 + ABZR87_RS08985 0.52 -1.7 1,907,023 + ABZR87_RS08985 0.52 -0.2 1,907,023 + ABZR87_RS08985 0.52 -1.6 1,907,024 - ABZR87_RS08985 0.52 -1.5 1,907,024 - ABZR87_RS08985 0.52 -0.6 1,907,024 - ABZR87_RS08985 0.52 -0.7 1,907,024 - ABZR87_RS08985 0.52 -0.4 1,907,024 - ABZR87_RS08985 0.52 -1.3 1,907,024 - ABZR87_RS08985 0.52 -2.6 1,907,145 + ABZR87_RS08985 0.61 -0.2 1,907,146 - ABZR87_RS08985 0.61 -2.5 1,907,146 - ABZR87_RS08985 0.61 +0.3 1,907,146 - ABZR87_RS08985 0.61 -1.5 1,907,155 + ABZR87_RS08985 0.62 -2.8 1,907,155 + ABZR87_RS08985 0.62 -0.7 1,907,155 + ABZR87_RS08985 0.62 -0.6 1,907,156 - ABZR87_RS08985 0.62 -1.9 1,907,245 + ABZR87_RS08985 0.69 -2.3 1,907,245 + ABZR87_RS08985 0.69 -0.5 1,907,245 + ABZR87_RS08985 0.69 -0.6 1,907,245 + ABZR87_RS08985 0.69 -0.4 1,907,246 - ABZR87_RS08985 0.69 -1.1 1,907,246 - ABZR87_RS08985 0.69 -0.8 1,907,246 - ABZR87_RS08985 0.69 -1.5 1,907,247 + ABZR87_RS08985 0.69 -0.9 1,907,247 + ABZR87_RS08985 0.69 -0.3 1,907,247 + ABZR87_RS08985 0.69 -0.3 1,907,248 - ABZR87_RS08985 0.69 +0.2 1,907,248 - ABZR87_RS08985 0.69 +0.0 1,907,248 - ABZR87_RS08985 0.69 +1.1 1,907,248 - ABZR87_RS08985 0.69 -0.4 1,907,248 - ABZR87_RS08985 0.69 -1.9 1,907,248 - ABZR87_RS08985 0.69 -1.6 1,907,248 - ABZR87_RS08985 0.69 -2.6 1,907,248 - ABZR87_RS08985 0.69 -1.2 1,907,248 - ABZR87_RS08985 0.69 -0.8 1,907,251 + ABZR87_RS08985 0.69 +0.3 1,907,251 + ABZR87_RS08985 0.69 -0.6 1,907,252 - ABZR87_RS08985 0.70 +0.6 1,907,252 - ABZR87_RS08985 0.70 +2.2
Or see this region's nucleotide sequence