Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS06495 and ABZR87_RS06500 are separated by 4 nucleotides ABZR87_RS06500 and ABZR87_RS06505 are separated by 101 nucleotides ABZR87_RS06505 and ABZR87_RS06510 overlap by 4 nucleotides
ABZR87_RS06495: ABZR87_RS06495 - D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase, at 1,383,082 to 1,383,579
_RS06495
ABZR87_RS06500: ABZR87_RS06500 - SPOR domain-containing protein, at 1,383,584 to 1,384,357
_RS06500
ABZR87_RS06505: ABZR87_RS06505 - type III pantothenate kinase, at 1,384,459 to 1,385,340
_RS06505
ABZR87_RS06510: ABZR87_RS06510 - biotin--[acetyl-CoA-carboxylase] ligase, at 1,385,337 to 1,386,218
_RS06510
Position (kb)
1384
1385
1386 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1383.492 kb on + strand, within ABZR87_RS06495 at 1383.493 kb on - strand, within ABZR87_RS06495 at 1383.493 kb on - strand, within ABZR87_RS06495 at 1383.493 kb on - strand, within ABZR87_RS06495 at 1383.586 kb on - strand at 1383.832 kb on + strand, within ABZR87_RS06500 at 1383.832 kb on + strand, within ABZR87_RS06500 at 1383.832 kb on + strand, within ABZR87_RS06500 at 1383.832 kb on + strand, within ABZR87_RS06500 at 1383.832 kb on + strand, within ABZR87_RS06500 at 1383.833 kb on - strand, within ABZR87_RS06500 at 1383.833 kb on - strand, within ABZR87_RS06500 at 1383.833 kb on - strand, within ABZR87_RS06500 at 1384.158 kb on - strand, within ABZR87_RS06500 at 1384.420 kb on + strand at 1384.421 kb on - strand at 1384.460 kb on + strand at 1384.461 kb on - strand at 1386.171 kb on - strand at 1386.218 kb on + strand at 1386.218 kb on + strand at 1386.218 kb on + strand at 1386.218 kb on + strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.219 kb on - strand at 1386.262 kb on + strand at 1386.262 kb on + strand at 1386.263 kb on - strand at 1386.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 1,383,492 + ABZR87_RS06495 0.82 +4.5 1,383,493 - ABZR87_RS06495 0.83 +2.7 1,383,493 - ABZR87_RS06495 0.83 +1.4 1,383,493 - ABZR87_RS06495 0.83 +2.2 1,383,586 - +0.3 1,383,832 + ABZR87_RS06500 0.32 -0.8 1,383,832 + ABZR87_RS06500 0.32 +1.0 1,383,832 + ABZR87_RS06500 0.32 +0.2 1,383,832 + ABZR87_RS06500 0.32 +1.2 1,383,832 + ABZR87_RS06500 0.32 -0.3 1,383,833 - ABZR87_RS06500 0.32 +0.1 1,383,833 - ABZR87_RS06500 0.32 +1.5 1,383,833 - ABZR87_RS06500 0.32 +0.1 1,384,158 - ABZR87_RS06500 0.74 -1.8 1,384,420 + +0.4 1,384,421 - -0.1 1,384,460 + -1.4 1,384,461 - +1.9 1,386,171 - -1.6 1,386,218 + +2.1 1,386,218 + -0.4 1,386,218 + +0.9 1,386,218 + +1.9 1,386,219 - -1.3 1,386,219 - -0.2 1,386,219 - -1.9 1,386,219 - -0.3 1,386,219 - +0.9 1,386,219 - +0.2 1,386,219 - -2.1 1,386,262 + -1.9 1,386,262 + -1.1 1,386,263 - +0.1 1,386,263 - -0.3
Or see this region's nucleotide sequence