Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS06235 and ABZR87_RS06240 are separated by 12 nucleotides ABZR87_RS06240 and ABZR87_RS06245 are separated by 10 nucleotides ABZR87_RS06245 and ABZR87_RS06250 are separated by 22 nucleotides ABZR87_RS06250 and ABZR87_RS06255 are separated by 1 nucleotides
ABZR87_RS06235: ABZR87_RS06235 - MBL fold metallo-hydrolase, at 1,332,104 to 1,333,057
_RS06235
ABZR87_RS06240: ABZR87_RS06240 - DUF1289 domain-containing protein, at 1,333,070 to 1,333,336
_RS06240
ABZR87_RS06245: ABZR87_RS06245 - YbaK/EbsC family protein, at 1,333,347 to 1,333,847
_RS06245
ABZR87_RS06250: ABZR87_RS06250 - hydroxymethylglutaryl-CoA lyase, at 1,333,870 to 1,334,799
_RS06250
ABZR87_RS06255: ABZR87_RS06255 - glyoxylate/hydroxypyruvate reductase A, at 1,334,801 to 1,335,742
_RS06255
Position (kb)
1333
1334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1332.357 kb on + strand, within ABZR87_RS06235 at 1332.357 kb on + strand, within ABZR87_RS06235 at 1332.358 kb on - strand, within ABZR87_RS06235 at 1332.726 kb on + strand, within ABZR87_RS06235 at 1332.726 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.798 kb on + strand, within ABZR87_RS06235 at 1332.799 kb on - strand, within ABZR87_RS06235 at 1332.799 kb on - strand, within ABZR87_RS06235 at 1332.799 kb on - strand, within ABZR87_RS06235 at 1333.090 kb on + strand at 1333.459 kb on + strand, within ABZR87_RS06245 at 1333.459 kb on + strand, within ABZR87_RS06245 at 1333.459 kb on + strand, within ABZR87_RS06245 at 1333.535 kb on - strand, within ABZR87_RS06245 at 1333.833 kb on - strand at 1333.875 kb on + strand at 1333.876 kb on - strand at 1334.030 kb on + strand, within ABZR87_RS06250 at 1334.030 kb on + strand, within ABZR87_RS06250 at 1334.030 kb on + strand, within ABZR87_RS06250 at 1334.031 kb on - strand, within ABZR87_RS06250 at 1334.031 kb on - strand, within ABZR87_RS06250 at 1334.076 kb on - strand, within ABZR87_RS06250 at 1334.165 kb on + strand, within ABZR87_RS06250 at 1334.165 kb on + strand, within ABZR87_RS06250 at 1334.165 kb on + strand, within ABZR87_RS06250 at 1334.351 kb on + strand, within ABZR87_RS06250 at 1334.570 kb on + strand, within ABZR87_RS06250 at 1334.570 kb on + strand, within ABZR87_RS06250 at 1334.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 1,332,357 + ABZR87_RS06235 0.27 -0.5 1,332,357 + ABZR87_RS06235 0.27 -0.3 1,332,358 - ABZR87_RS06235 0.27 -0.9 1,332,726 + ABZR87_RS06235 0.65 -0.5 1,332,726 + ABZR87_RS06235 0.65 +0.6 1,332,798 + ABZR87_RS06235 0.73 +0.3 1,332,798 + ABZR87_RS06235 0.73 +1.6 1,332,798 + ABZR87_RS06235 0.73 -1.4 1,332,798 + ABZR87_RS06235 0.73 -0.0 1,332,798 + ABZR87_RS06235 0.73 -0.8 1,332,798 + ABZR87_RS06235 0.73 +1.7 1,332,798 + ABZR87_RS06235 0.73 -2.0 1,332,799 - ABZR87_RS06235 0.73 -1.1 1,332,799 - ABZR87_RS06235 0.73 -0.9 1,332,799 - ABZR87_RS06235 0.73 -2.2 1,333,090 + +0.5 1,333,459 + ABZR87_RS06245 0.22 +0.2 1,333,459 + ABZR87_RS06245 0.22 +0.5 1,333,459 + ABZR87_RS06245 0.22 +0.3 1,333,535 - ABZR87_RS06245 0.38 +1.8 1,333,833 - -1.2 1,333,875 + -1.4 1,333,876 - +1.1 1,334,030 + ABZR87_RS06250 0.17 -1.5 1,334,030 + ABZR87_RS06250 0.17 -1.1 1,334,030 + ABZR87_RS06250 0.17 -0.5 1,334,031 - ABZR87_RS06250 0.17 +0.1 1,334,031 - ABZR87_RS06250 0.17 -0.9 1,334,076 - ABZR87_RS06250 0.22 -0.8 1,334,165 + ABZR87_RS06250 0.32 -0.1 1,334,165 + ABZR87_RS06250 0.32 -0.3 1,334,165 + ABZR87_RS06250 0.32 -2.2 1,334,351 + ABZR87_RS06250 0.52 -2.8 1,334,570 + ABZR87_RS06250 0.75 -1.3 1,334,570 + ABZR87_RS06250 0.75 -0.3 1,334,805 + -0.0
Or see this region's nucleotide sequence