Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS03420 and ABZR87_RS03425 overlap by 20 nucleotides ABZR87_RS03425 and ABZR87_RS03430 are separated by 1 nucleotides ABZR87_RS03430 and ABZR87_RS03435 are separated by 164 nucleotides
ABZR87_RS03420: ABZR87_RS03420 - type II secretion system minor pseudopilin GspI, at 713,754 to 714,164
_RS03420
ABZR87_RS03425: ABZR87_RS03425 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 714,145 to 714,888
_RS03425
ABZR87_RS03430: ABZR87_RS03430 - type II secretion system minor pseudopilin GspK, at 714,890 to 715,969
_RS03430
ABZR87_RS03435: ABZR87_RS03435 - type II secretion system protein GspL, at 716,134 to 717,570
_RS03435
Position (kb) 714
715
716 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 714.014 kb on + strand, within ABZR87_RS03420 at 714.162 kb on + strand at 714.162 kb on + strand at 714.162 kb on + strand at 714.162 kb on + strand at 714.162 kb on + strand at 714.162 kb on + strand at 714.163 kb on - strand at 714.163 kb on - strand at 714.163 kb on - strand at 714.334 kb on + strand, within ABZR87_RS03425 at 714.334 kb on + strand, within ABZR87_RS03425 at 714.334 kb on + strand, within ABZR87_RS03425 at 714.335 kb on - strand, within ABZR87_RS03425 at 714.335 kb on - strand, within ABZR87_RS03425 at 714.479 kb on - strand, within ABZR87_RS03425 at 714.479 kb on - strand, within ABZR87_RS03425 at 714.592 kb on + strand, within ABZR87_RS03425 at 714.592 kb on + strand, within ABZR87_RS03425 at 714.593 kb on - strand, within ABZR87_RS03425 at 714.658 kb on + strand, within ABZR87_RS03425 at 714.738 kb on + strand, within ABZR87_RS03425 at 714.738 kb on + strand, within ABZR87_RS03425 at 714.738 kb on + strand, within ABZR87_RS03425 at 714.738 kb on + strand, within ABZR87_RS03425 at 714.739 kb on - strand, within ABZR87_RS03425 at 714.739 kb on - strand, within ABZR87_RS03425 at 715.026 kb on + strand, within ABZR87_RS03430 at 715.026 kb on + strand, within ABZR87_RS03430 at 715.027 kb on - strand, within ABZR87_RS03430 at 715.244 kb on + strand, within ABZR87_RS03430 at 715.244 kb on + strand, within ABZR87_RS03430 at 715.244 kb on + strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.245 kb on - strand, within ABZR87_RS03430 at 715.364 kb on + strand, within ABZR87_RS03430 at 715.365 kb on - strand, within ABZR87_RS03430 at 715.365 kb on - strand, within ABZR87_RS03430 at 715.424 kb on + strand, within ABZR87_RS03430 at 715.424 kb on + strand, within ABZR87_RS03430 at 715.424 kb on + strand, within ABZR87_RS03430 at 715.424 kb on + strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.425 kb on - strand, within ABZR87_RS03430 at 715.574 kb on + strand, within ABZR87_RS03430 at 715.607 kb on + strand, within ABZR87_RS03430 at 715.607 kb on + strand, within ABZR87_RS03430 at 715.608 kb on - strand, within ABZR87_RS03430 at 715.608 kb on - strand, within ABZR87_RS03430 at 715.665 kb on + strand, within ABZR87_RS03430 at 715.665 kb on + strand, within ABZR87_RS03430 at 715.666 kb on - strand, within ABZR87_RS03430 at 715.736 kb on + strand, within ABZR87_RS03430 at 715.736 kb on + strand, within ABZR87_RS03430 at 715.737 kb on - strand, within ABZR87_RS03430 at 715.737 kb on - strand, within ABZR87_RS03430 at 715.737 kb on - strand, within ABZR87_RS03430 at 715.737 kb on - strand, within ABZR87_RS03430 at 715.747 kb on + strand, within ABZR87_RS03430 at 715.747 kb on + strand, within ABZR87_RS03430 at 715.835 kb on + strand, within ABZR87_RS03430 at 715.835 kb on + strand, within ABZR87_RS03430 at 715.847 kb on + strand, within ABZR87_RS03430 at 715.848 kb on - strand, within ABZR87_RS03430 at 715.848 kb on - strand, within ABZR87_RS03430 at 716.001 kb on - strand at 716.013 kb on + strand at 716.036 kb on + strand at 716.036 kb on + strand at 716.036 kb on + strand at 716.036 kb on + strand at 716.036 kb on + strand at 716.037 kb on - strand at 716.037 kb on - strand at 716.037 kb on - strand at 716.037 kb on - strand at 716.122 kb on + strand at 716.122 kb on + strand at 716.122 kb on + strand at 716.123 kb on - strand at 716.123 kb on - strand at 716.123 kb on - strand at 716.123 kb on - strand at 716.149 kb on + strand at 716.149 kb on + strand at 716.149 kb on + strand at 716.150 kb on - strand at 716.150 kb on - strand at 716.150 kb on - strand at 716.150 kb on - strand at 716.150 kb on - strand at 716.150 kb on - strand at 716.448 kb on + strand, within ABZR87_RS03435 at 716.565 kb on + strand, within ABZR87_RS03435 at 716.566 kb on - strand, within ABZR87_RS03435 at 716.566 kb on - strand, within ABZR87_RS03435 at 716.566 kb on - strand, within ABZR87_RS03435 at 716.566 kb on - strand, within ABZR87_RS03435 at 716.566 kb on - strand, within ABZR87_RS03435 at 716.837 kb on + strand, within ABZR87_RS03435 at 716.837 kb on + strand, within ABZR87_RS03435 at 716.838 kb on - strand, within ABZR87_RS03435 at 716.838 kb on - strand, within ABZR87_RS03435 at 716.838 kb on - strand, within ABZR87_RS03435
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 714,014 + ABZR87_RS03420 0.63 -2.4 714,162 + -1.0 714,162 + +0.8 714,162 + +0.9 714,162 + -0.9 714,162 + +0.5 714,162 + -0.1 714,163 - -1.2 714,163 - +0.0 714,163 - -0.6 714,334 + ABZR87_RS03425 0.25 -1.1 714,334 + ABZR87_RS03425 0.25 -2.4 714,334 + ABZR87_RS03425 0.25 -1.7 714,335 - ABZR87_RS03425 0.26 -0.6 714,335 - ABZR87_RS03425 0.26 -0.5 714,479 - ABZR87_RS03425 0.45 -0.7 714,479 - ABZR87_RS03425 0.45 -0.4 714,592 + ABZR87_RS03425 0.60 -1.9 714,592 + ABZR87_RS03425 0.60 -1.4 714,593 - ABZR87_RS03425 0.60 -1.4 714,658 + ABZR87_RS03425 0.69 +0.5 714,738 + ABZR87_RS03425 0.80 -1.6 714,738 + ABZR87_RS03425 0.80 -1.5 714,738 + ABZR87_RS03425 0.80 +0.2 714,738 + ABZR87_RS03425 0.80 -1.5 714,739 - ABZR87_RS03425 0.80 -0.1 714,739 - ABZR87_RS03425 0.80 -0.7 715,026 + ABZR87_RS03430 0.13 -1.4 715,026 + ABZR87_RS03430 0.13 +0.1 715,027 - ABZR87_RS03430 0.13 -0.4 715,244 + ABZR87_RS03430 0.33 -0.6 715,244 + ABZR87_RS03430 0.33 -0.4 715,244 + ABZR87_RS03430 0.33 -1.5 715,245 - ABZR87_RS03430 0.33 +1.5 715,245 - ABZR87_RS03430 0.33 -1.5 715,245 - ABZR87_RS03430 0.33 -1.4 715,245 - ABZR87_RS03430 0.33 +0.6 715,245 - ABZR87_RS03430 0.33 -1.8 715,245 - ABZR87_RS03430 0.33 -0.6 715,245 - ABZR87_RS03430 0.33 -1.1 715,245 - ABZR87_RS03430 0.33 +1.5 715,364 + ABZR87_RS03430 0.44 +0.4 715,365 - ABZR87_RS03430 0.44 -1.7 715,365 - ABZR87_RS03430 0.44 -1.3 715,424 + ABZR87_RS03430 0.49 -0.8 715,424 + ABZR87_RS03430 0.49 -1.0 715,424 + ABZR87_RS03430 0.49 +2.3 715,424 + ABZR87_RS03430 0.49 -0.5 715,425 - ABZR87_RS03430 0.50 -1.1 715,425 - ABZR87_RS03430 0.50 -0.6 715,425 - ABZR87_RS03430 0.50 -0.6 715,425 - ABZR87_RS03430 0.50 -1.9 715,425 - ABZR87_RS03430 0.50 +0.3 715,425 - ABZR87_RS03430 0.50 -0.1 715,574 + ABZR87_RS03430 0.63 -3.5 715,607 + ABZR87_RS03430 0.66 +0.2 715,607 + ABZR87_RS03430 0.66 -3.0 715,608 - ABZR87_RS03430 0.66 -3.6 715,608 - ABZR87_RS03430 0.66 +0.2 715,665 + ABZR87_RS03430 0.72 -0.6 715,665 + ABZR87_RS03430 0.72 -1.3 715,666 - ABZR87_RS03430 0.72 -0.1 715,736 + ABZR87_RS03430 0.78 -1.2 715,736 + ABZR87_RS03430 0.78 +0.4 715,737 - ABZR87_RS03430 0.78 +1.3 715,737 - ABZR87_RS03430 0.78 -0.1 715,737 - ABZR87_RS03430 0.78 -1.1 715,737 - ABZR87_RS03430 0.78 +0.6 715,747 + ABZR87_RS03430 0.79 -2.5 715,747 + ABZR87_RS03430 0.79 -1.4 715,835 + ABZR87_RS03430 0.88 -1.5 715,835 + ABZR87_RS03430 0.88 -1.0 715,847 + ABZR87_RS03430 0.89 +0.5 715,848 - ABZR87_RS03430 0.89 -1.6 715,848 - ABZR87_RS03430 0.89 -1.9 716,001 - -2.3 716,013 + -2.8 716,036 + -0.3 716,036 + +0.1 716,036 + +0.3 716,036 + +1.3 716,036 + +1.2 716,037 - -0.2 716,037 - +0.0 716,037 - -0.6 716,037 - -1.5 716,122 + -2.0 716,122 + +0.6 716,122 + +0.1 716,123 - -0.0 716,123 - -1.7 716,123 - -0.8 716,123 - -2.8 716,149 + -1.1 716,149 + +0.2 716,149 + -0.2 716,150 - -0.8 716,150 - -1.7 716,150 - -1.4 716,150 - -0.2 716,150 - +0.3 716,150 - +0.9 716,448 + ABZR87_RS03435 0.22 +0.7 716,565 + ABZR87_RS03435 0.30 -0.1 716,566 - ABZR87_RS03435 0.30 +0.6 716,566 - ABZR87_RS03435 0.30 -0.7 716,566 - ABZR87_RS03435 0.30 -2.8 716,566 - ABZR87_RS03435 0.30 -0.7 716,566 - ABZR87_RS03435 0.30 -0.4 716,837 + ABZR87_RS03435 0.49 +1.0 716,837 + ABZR87_RS03435 0.49 -0.9 716,838 - ABZR87_RS03435 0.49 -1.0 716,838 - ABZR87_RS03435 0.49 -1.4 716,838 - ABZR87_RS03435 0.49 -1.2
Or see this region's nucleotide sequence