Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00855 and ABZR87_RS00860 overlap by 1 nucleotides ABZR87_RS00860 and ABZR87_RS00865 are separated by 3 nucleotides ABZR87_RS00865 and ABZR87_RS00870 are separated by 125 nucleotides
ABZR87_RS00855: ABZR87_RS00855 - murein biosynthesis integral membrane protein MurJ, at 171,328 to 172,881
_RS00855
ABZR87_RS00860: ABZR87_RS00860 - SirB1 family protein, at 172,881 to 173,726
_RS00860
ABZR87_RS00865: ABZR87_RS00865 - VTT domain-containing protein, at 173,730 to 174,374
_RS00865
ABZR87_RS00870: ABZR87_RS00870 - DNA mismatch repair endonuclease MutL, at 174,500 to 176,407
_RS00870
Position (kb) 172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 172.889 kb on + strand at 172.889 kb on + strand at 172.889 kb on + strand at 172.889 kb on + strand at 172.889 kb on + strand at 173.100 kb on + strand, within ABZR87_RS00860 at 173.136 kb on + strand, within ABZR87_RS00860 at 173.137 kb on - strand, within ABZR87_RS00860 at 173.139 kb on + strand, within ABZR87_RS00860 at 173.139 kb on + strand, within ABZR87_RS00860 at 173.139 kb on + strand, within ABZR87_RS00860 at 173.139 kb on + strand, within ABZR87_RS00860 at 173.139 kb on + strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.140 kb on - strand, within ABZR87_RS00860 at 173.157 kb on + strand, within ABZR87_RS00860 at 173.157 kb on + strand, within ABZR87_RS00860 at 173.158 kb on - strand, within ABZR87_RS00860 at 173.209 kb on + strand, within ABZR87_RS00860 at 173.373 kb on + strand, within ABZR87_RS00860 at 173.374 kb on - strand, within ABZR87_RS00860 at 173.487 kb on + strand, within ABZR87_RS00860 at 173.487 kb on + strand, within ABZR87_RS00860 at 173.487 kb on + strand, within ABZR87_RS00860 at 173.488 kb on - strand, within ABZR87_RS00860 at 173.488 kb on - strand, within ABZR87_RS00860 at 173.488 kb on - strand, within ABZR87_RS00860 at 173.589 kb on + strand, within ABZR87_RS00860 at 173.637 kb on + strand, within ABZR87_RS00860 at 173.638 kb on - strand, within ABZR87_RS00860 at 173.638 kb on - strand, within ABZR87_RS00860 at 173.638 kb on - strand, within ABZR87_RS00860 at 173.732 kb on - strand at 173.732 kb on - strand at 173.858 kb on + strand, within ABZR87_RS00865 at 174.429 kb on + strand at 174.429 kb on + strand at 174.430 kb on - strand at 174.430 kb on - strand at 174.488 kb on + strand at 174.488 kb on + strand at 174.488 kb on + strand at 174.488 kb on + strand at 174.488 kb on + strand at 174.488 kb on + strand at 174.489 kb on - strand at 174.489 kb on - strand at 174.489 kb on - strand at 174.489 kb on - strand at 174.489 kb on - strand at 174.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 172,889 + +0.2 172,889 + +1.0 172,889 + +0.1 172,889 + -0.4 172,889 + +0.5 173,100 + ABZR87_RS00860 0.26 -0.4 173,136 + ABZR87_RS00860 0.30 -0.6 173,137 - ABZR87_RS00860 0.30 +1.0 173,139 + ABZR87_RS00860 0.30 +0.2 173,139 + ABZR87_RS00860 0.30 -0.1 173,139 + ABZR87_RS00860 0.30 -0.1 173,139 + ABZR87_RS00860 0.30 +0.8 173,139 + ABZR87_RS00860 0.30 -0.7 173,140 - ABZR87_RS00860 0.31 -0.1 173,140 - ABZR87_RS00860 0.31 -1.4 173,140 - ABZR87_RS00860 0.31 +1.0 173,140 - ABZR87_RS00860 0.31 -2.8 173,140 - ABZR87_RS00860 0.31 -0.9 173,140 - ABZR87_RS00860 0.31 -0.6 173,157 + ABZR87_RS00860 0.33 -0.8 173,157 + ABZR87_RS00860 0.33 -1.3 173,158 - ABZR87_RS00860 0.33 -1.4 173,209 + ABZR87_RS00860 0.39 +0.3 173,373 + ABZR87_RS00860 0.58 -1.4 173,374 - ABZR87_RS00860 0.58 -0.9 173,487 + ABZR87_RS00860 0.72 +0.0 173,487 + ABZR87_RS00860 0.72 -0.1 173,487 + ABZR87_RS00860 0.72 -0.4 173,488 - ABZR87_RS00860 0.72 -0.2 173,488 - ABZR87_RS00860 0.72 -1.4 173,488 - ABZR87_RS00860 0.72 -1.5 173,589 + ABZR87_RS00860 0.84 +0.3 173,637 + ABZR87_RS00860 0.89 -0.9 173,638 - ABZR87_RS00860 0.89 -0.1 173,638 - ABZR87_RS00860 0.89 +1.0 173,638 - ABZR87_RS00860 0.89 -2.5 173,732 - -0.2 173,732 - -3.3 173,858 + ABZR87_RS00865 0.20 -2.1 174,429 + +2.3 174,429 + +1.5 174,430 - +2.0 174,430 - -1.5 174,488 + -2.2 174,488 + +0.7 174,488 + -2.3 174,488 + +0.5 174,488 + -0.5 174,488 + -1.4 174,489 - +1.5 174,489 - -0.2 174,489 - -2.5 174,489 - -0.8 174,489 - +1.1 174,489 - +0.7
Or see this region's nucleotide sequence