Experiment: root EcoFAB sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS00395 and ABZR87_RS00400 are separated by 109 nucleotides ABZR87_RS00400 and ABZR87_RS00405 overlap by 1 nucleotides ABZR87_RS00405 and ABZR87_RS00410 are separated by 7 nucleotides ABZR87_RS00410 and ABZR87_RS00415 are separated by 3 nucleotides
ABZR87_RS00395: ABZR87_RS00395 - bifunctional riboflavin kinase/FAD synthetase, at 81,322 to 82,320
_RS00395
ABZR87_RS00400: ABZR87_RS00400 - isoleucine--tRNA ligase, at 82,430 to 85,309
_RS00400
ABZR87_RS00405: ABZR87_RS00405 - signal peptidase II, at 85,309 to 85,833
_RS00405
ABZR87_RS00410: ABZR87_RS00410 - DUF4180 domain-containing protein, at 85,841 to 86,218
_RS00410
ABZR87_RS00415: ABZR87_RS00415 - YdcF family protein, at 86,222 to 86,800
_RS00415
Position (kb) 82
83
84
85
86 Strain fitness (log2 ratio) -2
-1
0
1
2 at 82.318 kb on + strand at 86.057 kb on + strand, within ABZR87_RS00410 at 86.058 kb on - strand, within ABZR87_RS00410 at 86.058 kb on - strand, within ABZR87_RS00410 at 86.058 kb on - strand, within ABZR87_RS00410 at 86.233 kb on - strand at 86.244 kb on + strand at 86.244 kb on + strand at 86.245 kb on - strand at 86.262 kb on + strand at 86.263 kb on - strand at 86.263 kb on - strand at 86.263 kb on - strand at 86.263 kb on - strand at 86.289 kb on + strand, within ABZR87_RS00415 at 86.289 kb on + strand, within ABZR87_RS00415 at 86.289 kb on + strand, within ABZR87_RS00415 at 86.289 kb on + strand, within ABZR87_RS00415 at 86.290 kb on - strand, within ABZR87_RS00415
Per-strain Table
Position Strand Gene LocusTag Fraction root EcoFAB sample 2; outgrowth in LB remove 82,318 + -1.5 86,057 + ABZR87_RS00410 0.57 -0.6 86,058 - ABZR87_RS00410 0.57 +0.3 86,058 - ABZR87_RS00410 0.57 -0.7 86,058 - ABZR87_RS00410 0.57 +0.0 86,233 - -2.7 86,244 + +2.5 86,244 + -0.2 86,245 - -0.1 86,262 + -1.4 86,263 - +0.5 86,263 - +0.7 86,263 - -0.7 86,263 - +0.6 86,289 + ABZR87_RS00415 0.12 +0.1 86,289 + ABZR87_RS00415 0.12 -0.6 86,289 + ABZR87_RS00415 0.12 -1.2 86,289 + ABZR87_RS00415 0.12 +0.2 86,290 - ABZR87_RS00415 0.12 -1.3
Or see this region's nucleotide sequence