Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3222 and Shew_3223 are separated by 175 nucleotides Shew_3223 and Shew_3224 are separated by 3 nucleotides Shew_3224 and Shew_3225 are separated by 38 nucleotides Shew_3225 and Shew_3226 are separated by 12 nucleotides
Shew_3222: Shew_3222 - protein of unknown function DUF335, SprT (RefSeq), at 3,845,775 to 3,846,386
_3222
Shew_3223: Shew_3223 - deoxyribonuclease I (RefSeq), at 3,846,562 to 3,847,317
_3223
Shew_3224: Shew_3224 - hypothetical protein (RefSeq), at 3,847,321 to 3,848,052
_3224
Shew_3225: Shew_3225 - glutathione synthetase (RefSeq), at 3,848,091 to 3,849,038
_3225
Shew_3226: Shew_3226 - alkaline phosphatase (RefSeq), at 3,849,051 to 3,850,682
_3226
Position (kb)
3847
3848
3849 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3846.670 kb on - strand, within Shew_3223 at 3847.082 kb on - strand, within Shew_3223 at 3847.254 kb on + strand at 3847.275 kb on - strand at 3847.352 kb on + strand at 3847.733 kb on + strand, within Shew_3224 at 3847.836 kb on + strand, within Shew_3224 at 3847.836 kb on + strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.844 kb on - strand, within Shew_3224 at 3847.934 kb on + strand, within Shew_3224 at 3847.997 kb on - strand at 3848.197 kb on + strand, within Shew_3225 at 3848.753 kb on - strand, within Shew_3225 at 3848.866 kb on + strand, within Shew_3225 at 3848.956 kb on + strand at 3848.956 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,846,670 - Shew_3223 0.14 -1.4 3,847,082 - Shew_3223 0.69 -1.4 3,847,254 + -0.1 3,847,275 - -2.2 3,847,352 + -0.4 3,847,733 + Shew_3224 0.56 -0.3 3,847,836 + Shew_3224 0.70 -0.5 3,847,836 + Shew_3224 0.70 -0.2 3,847,844 - Shew_3224 0.71 -1.7 3,847,844 - Shew_3224 0.71 -3.3 3,847,844 - Shew_3224 0.71 -2.1 3,847,934 + Shew_3224 0.84 -0.1 3,847,997 - -2.5 3,848,197 + Shew_3225 0.11 -3.5 3,848,753 - Shew_3225 0.70 -4.6 3,848,866 + Shew_3225 0.82 -3.0 3,848,956 + -2.8 3,848,956 + -2.9
Or see this region's nucleotide sequence