Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2823 and rpsO are separated by 107 nucleotides rpsO and Shew_2825 are separated by 95 nucleotides Shew_2825 and rbfA overlap by 1 nucleotides rbfA and infB are separated by 124 nucleotides
Shew_2823: Shew_2823 - diguanylate cyclase/phosphodiesterase (RefSeq), at 3,346,124 to 3,348,157
_2823
Shew_2824: rpsO - 30S ribosomal protein S15 (RefSeq), at 3,348,265 to 3,348,534
rpsO
Shew_2825: Shew_2825 - tRNA pseudouridine synthase B (RefSeq), at 3,348,630 to 3,349,577
_2825
Shew_2826: rbfA - ribosome-binding factor A (RefSeq), at 3,349,577 to 3,349,999
rbfA
Shew_2827: infB - translation initiation factor IF-2 (RefSeq), at 3,350,124 to 3,352,781
infB
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-1
0
1 at 3347.735 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3348.775 kb on + strand, within Shew_2825 at 3349.000 kb on + strand, within Shew_2825 at 3349.095 kb on - strand, within Shew_2825 at 3349.489 kb on - strand at 3349.535 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,347,735 + Shew_2823 0.79 -0.0 3,347,815 + Shew_2823 0.83 -0.0 3,347,815 + Shew_2823 0.83 +0.1 3,347,815 + Shew_2823 0.83 -0.5 3,348,775 + Shew_2825 0.15 -0.6 3,349,000 + Shew_2825 0.39 -1.0 3,349,095 - Shew_2825 0.49 -1.6 3,349,489 - +0.1 3,349,535 + -0.5
Or see this region's nucleotide sequence