Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2724 and Shew_2725 overlap by 7 nucleotides Shew_2725 and Shew_2726 are separated by 330 nucleotides Shew_2726 and Shew_2727 are separated by 167 nucleotides
Shew_2724: Shew_2724 - indigoidine synthase A family protein (RefSeq), at 3,223,621 to 3,224,535
_2724
Shew_2725: Shew_2725 - ribokinase-like domain-containing protein (RefSeq), at 3,224,529 to 3,225,620
_2725
Shew_2726: Shew_2726 - hypothetical protein (RefSeq), at 3,225,951 to 3,226,403
_2726
Shew_2727: Shew_2727 - hypothetical protein (RefSeq), at 3,226,571 to 3,227,269
_2727
Position (kb)
3224
3225
3226 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3223.911 kb on + strand, within Shew_2724 at 3223.911 kb on + strand, within Shew_2724 at 3223.919 kb on - strand, within Shew_2724 at 3224.291 kb on + strand, within Shew_2724 at 3224.299 kb on - strand, within Shew_2724 at 3224.402 kb on - strand, within Shew_2724 at 3224.458 kb on + strand at 3224.875 kb on + strand, within Shew_2725 at 3225.205 kb on - strand, within Shew_2725 at 3225.347 kb on + strand, within Shew_2725 at 3225.469 kb on - strand, within Shew_2725 at 3225.646 kb on - strand at 3225.910 kb on + strand at 3226.131 kb on - strand, within Shew_2726 at 3226.168 kb on - strand, within Shew_2726 at 3226.168 kb on - strand, within Shew_2726 at 3226.168 kb on - strand, within Shew_2726 at 3226.168 kb on - strand, within Shew_2726
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,223,911 + Shew_2724 0.32 +0.3 3,223,911 + Shew_2724 0.32 -0.1 3,223,919 - Shew_2724 0.33 -0.7 3,224,291 + Shew_2724 0.73 +0.6 3,224,299 - Shew_2724 0.74 -0.4 3,224,402 - Shew_2724 0.85 -0.1 3,224,458 + -0.0 3,224,875 + Shew_2725 0.32 +1.7 3,225,205 - Shew_2725 0.62 +0.1 3,225,347 + Shew_2725 0.75 +0.9 3,225,469 - Shew_2725 0.86 -0.2 3,225,646 - -0.0 3,225,910 + +3.7 3,226,131 - Shew_2726 0.40 +0.2 3,226,168 - Shew_2726 0.48 -0.2 3,226,168 - Shew_2726 0.48 -0.1 3,226,168 - Shew_2726 0.48 -0.5 3,226,168 - Shew_2726 0.48 -0.1
Or see this region's nucleotide sequence