Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0130 and Shew_0131 overlap by 8 nucleotides Shew_0131 and Shew_0132 are separated by 107 nucleotides Shew_0132 and Shew_0133 overlap by 10 nucleotides
Shew_0130: Shew_0130 - 3'(2'),5'-bisphosphate nucleotidase (RefSeq), at 139,630 to 140,442
_0130
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Shew_0132: Shew_0132 - TetR family transcriptional regulator (RefSeq), at 140,998 to 141,654
_0132
Shew_0133: Shew_0133 - phospholipid/glycerol acyltransferase (RefSeq), at 141,645 to 143,507
_0133
Position (kb) 140
141
142 Strain fitness (log2 ratio) -1
0
1 at 140.097 kb on - strand, within Shew_0130 at 140.201 kb on - strand, within Shew_0130 at 140.442 kb on + strand at 140.476 kb on - strand at 140.609 kb on - strand, within Shew_0131 at 140.665 kb on + strand, within Shew_0131 at 140.756 kb on - strand, within Shew_0131 at 140.855 kb on + strand at 141.028 kb on - strand at 141.097 kb on - strand, within Shew_0132 at 141.099 kb on + strand, within Shew_0132 at 141.276 kb on - strand, within Shew_0132 at 141.342 kb on + strand, within Shew_0132 at 141.471 kb on + strand, within Shew_0132 at 141.536 kb on + strand, within Shew_0132 at 141.570 kb on + strand, within Shew_0132 at 141.583 kb on - strand, within Shew_0132 at 141.886 kb on + strand, within Shew_0133 at 141.997 kb on + strand, within Shew_0133 at 142.032 kb on - strand, within Shew_0133 at 142.069 kb on + strand, within Shew_0133 at 142.270 kb on - strand, within Shew_0133 at 142.528 kb on + strand, within Shew_0133 at 142.536 kb on - strand, within Shew_0133 at 142.548 kb on + strand, within Shew_0133
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 140,097 - Shew_0130 0.57 -0.4 140,201 - Shew_0130 0.70 +0.3 140,442 + -0.1 140,476 - +0.3 140,609 - Shew_0131 0.38 -0.2 140,665 + Shew_0131 0.50 +0.0 140,756 - Shew_0131 0.70 -0.4 140,855 + +0.6 141,028 - -0.1 141,097 - Shew_0132 0.15 -0.0 141,099 + Shew_0132 0.15 -0.4 141,276 - Shew_0132 0.42 +0.1 141,342 + Shew_0132 0.52 -0.7 141,471 + Shew_0132 0.72 +0.2 141,536 + Shew_0132 0.82 -0.2 141,570 + Shew_0132 0.87 -0.3 141,583 - Shew_0132 0.89 +0.1 141,886 + Shew_0133 0.13 +0.2 141,997 + Shew_0133 0.19 +0.2 142,032 - Shew_0133 0.21 +0.5 142,069 + Shew_0133 0.23 +0.4 142,270 - Shew_0133 0.34 -0.1 142,528 + Shew_0133 0.47 -0.2 142,536 - Shew_0133 0.48 +0.1 142,548 + Shew_0133 0.48 +0.1
Or see this region's nucleotide sequence